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To establish a chromatogram-effect correlated quality evaluation system for the antibacterial activity of Flos Lonicerae extract by data analysis of components.
The best liquid chromatographic conditions were optimized by investigating the conditions of mobile phase, wavelength and flow rate, and the quantitative fingerprint of 50 batches of Flos Lonicerae extract was established. The best antibacterial concentration of the extract was obtained by microdilution method, and the antibacterial rates of 50 batches of samples were determined. Similarity analysis, cluster analysis, principal component analysis, grey correlation analysis and mathematical model establishment by support vector machine were used to analyze the quantitative fingerprints and antibacterial rates of 50 batches of samples.
Eighteen common peaks were selected from the 50 quantitative fingerprints of Flos Lonicerae extract, and the similarities were between 0.608-1. Six chemical components were identified (peak 4: neochlorogenic acid, peak 8: chlorogenic acid, peak 9: cryptochlorogenic acid, peak 16: isochlorogenic acid B, peak 17: isochlorogenic acid A and peak 18: isochlorogenic acid C). The average antibacterial rates of 50 batches of extracts were between 3.93%-70.50%. The results of principal component analysis and cluster analysis were highly consistent, and the results of grey correlation analysis showed that all components were positively correlated with antibacterial effect. The relative deviations between the predicted data of the mathematical model and the experimental data were all below 2%.
The HPLC conditions of Flos Lonicerae extract are stable and reliable, and the quality evaluation system of antibacterial activity of Flos Lonicerae extract established can evaluate its quality based on antibacterial rate.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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