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To establish a fast, accurate and sensitive method by UPLC-DAD-Q-TOF-MS/MS for the detection of 20 kinds of illegally added chemicals such as azilsartan, nifedipin, lacidipine、nebivolo in anti-hypertensive health foods.
Waters HSS T3 C18（100 mm×2.1 mm, 1.8 μm ） column was used with mobile phase of 0.01 mol·L-1 ammonium acetate aqueous solution-methanol and gradient elution（0 min, 5% A; 0-8 min, 5% A→95% A; 8-11 min, 95% A; 11-11.2 min, 95% A→5% A; 11.2-12 min, 5%). The flow rate was 0.3 mL·min-1. The injection volume was 1 μL. ESI+, ESI- scan and MS/MS mode were applied. Qualitative and quantitative analyses were carried out by UPLC-DAD-Q-TOF-MS/MS.
The method was validated. A good linear relationship was showed in the concentration of 1-50 μg·mL-1, （RSD<3%）and the recovery was obtained in range of 80.5%-98.7%. Illegally added chemical drugs were found in 15 batches of samples tested by this method. Two or more types of drugs were detected in 14 batches of samples. Torsemide, candesartan cilexetil, lacidipine were detected in 9 samples. Lacidipine and nebivolol were found in one of the sample.
This method is fast, accurate and sensitive with a high degree of separation. It can be used as a powerful tool for the fast detection of illegally added chemicals in anti-hypertensive health foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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