PDF
Full
Outline
To establish an UPLC-MS/MS method for simultaneous determination of 11 components(caffeic acid, afzelin, gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, salidroside, eleutheroside B, ginsenoside Re, ginsenoside Rg1, ginsenoside Rd) in Hongwushen capsules.
A Shim-pack GIST C18 chromatographic column(100 mm×2.1 mm, 2 μm) was used. The mobile phase was composed of acetonitrile -0.1% acetic acid -1 mmol·L-1 ammonium acetate aqueous solution and the gradient elution was applied. The flow rate was 0.3 mL·min-1, the column temperature was 30 ℃, and the injection volume was 1 μL. Electrospray ionization (ESI) source and multiple reaction monitoring (MRM) mode in both positive and negative were used for ion detections.
The linear relationship of 11 components was good in the concentration range, and the linear correlation coefficients were all above 0.999 0. The RSD values of precision were less than 3%. The repeatability and stability were good, and the RSD values were less than 5%. The average recoveries were in the range of 97.1%-101.5% with RSDs≤3.7%. The contents of caffeic acid, afzelin, gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, salidroside, eleutheroside B, ginsenoside Re, ginsenoside Rg1 and ginsenoside Rd in 10 batches of Hongwushen capsules were 0.010-0.013 mg·g-1, 0.000 7-0.001 4 mg·g-1, 0.035-0.038 mg·g-1, 0.312-0.315 mg·g-1, 0.413-0.417 mg·g-1, 0.411-0.416 mg·g-1, 4.355-4.358 mg·g-1, 0.030-0.032 mg·g-1, 0.993-0.999 mg·g-1, 1.120-1.124 mg·g-1 and 2.536-2.538 mg·g-1, respectively.
The method is accurate, sensitive, stable and reproducible, and can be used for the quality control of Hongwushen capsules.
PDF
97
47
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
