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To establish the RP-UPLC-PDA method for simultaneous determination of triptolide，triptonide, triptophenolide, wilforine, wilforlide A and celastrol in Tripterygii Radix.
Tripterygii Radix were extracted with ethyl acetate and the extracts were dissolved and separated by methanol. The six components were determined by RP-UPLC-PDA method. The chromatographic column was AcquityTM UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm), the column temperature was 30 ℃, the injection volumn was 2 μL, the flow rate was 0.4 mL·min-1 and the mobile phase was acetonitrile (A)-0.1% formic acid (B).
The linear relationship of six components was good (0.999 2≤r≤0.999 7) in the concentration ranges. The average recoveries were 99.2%-103.1% and RSDs were 1.2%-2.9%. The contents in 10 batches of Tripterygii Radix from different habitat were determined. The results showed that the contents of Tripterygii Radix in prepared pieces from different producing areas were different. The highest content of triptolide was 140.2 μg·g-1, and the lowest content was 103.2 μg·g-1. The highest content of triptonide was 224.7 μg·g-1 and the lowest content was 112.2 μg·g-1. The highest content of triptophenolide was 306.7 μg·g-1 and the lowest content was 189.6 μg·g-1. The highest and lowest contents of wilforine were 283.2 μg·g-1 and 211.2 μg·g-1. The highest content of wilfhabitat was 31.2 μg·g-1 and the lowest content was 16.8 μg·g-1. The highest content of celastrol was 87.6 μg·g-1, and the lowest content was 52.1 μg·g-1.
The RP-UPLC-PDA method can simultaneously determine six components in Tripterygii Radix. The method is reliable and stable, which is suitable for quantitative analysis and determination of Tripterygii Radix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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