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To establish an UPLC quantitative analysis method for the simultaneous determination of 13 components in Yankening tablets, including phellodendrine, coptisine, baicalin, palmatine, berberine, wogonoside, baicalein, aloe-emodin, rhein, wogonin, emodin, chrysophanol, physcion.
Agilent Eclipse Plus C18(100 mm×2.1 mm, 1.8 μm) column was used with acetonitrile -0.1% phosphoric acid as mobile phase, and gradient elution at a flow rate of 0.3 mL·min-1. The detection wavelengths were 210 nm and 254 nm. The column temperature was 40 ℃.
Phellodendrine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, berberine hydrochloride, baicalin, wogonoside, baicalein, aloe-emodin, rhein, wogonin, emodin, chrysophanol, physcion showed good linear relationships within their concentration range of 0.97-48.63 μg·mL-1, 0.95-47.52 μg·mL-1, 0.86-43.15 μg·mL-1, 0.86-43.19 μg·mL-1, 0.89-44.37 μg·mL-1, 1.00-49.84 μg·mL-1, 1.02-51.01 μg·mL-1, 0.97-48.31 μg·mL-1, 0.99-49.50 μg·mL-1, 1.04-51.80 μg·mL-1, 1.00-50.04 μg·mL-1, 1.00-49.80 μg·mL-1, 1.01-50.64 μg·mL-1. The average recoveries(n=6) were 95.2%, 96.7%, 95.9%, 98.3%, 94.1%，97.6%, 99.2%, 96.6%, 95.5%, 97.2%, 97.0%, 97.8%, 98.7%, RSD values were all less than 2.0%. The contents of the 13 chemical components in 3 batches of samples were 1.367-1.488 mg·g-1(calculated as phellodendrine hydrochloride), 0.378-0.412 mg·g-1(calculated as coptisine hydrochloride), 4.611-5.505 mg·g-1, 0.324-0.407 mg·g-1(calculated as palmatine hydrochloride), 3.665-3.878 mg·g-1(calculated as berberine hydrochloride), 1.107-1.682 mg·g-1, 0.392-0.941 mg·g-1, 0.076-0.105 mg·g-1, 0.097-0.116 mg·g-1, 1.059-1.213 mg·g-1, 0.149-0.167 mg·g-1, 0.213-0.239 mg·g-1, 0.047-0.059 mg·g-1.
The method is accurate, high analysis efficiency, good repeatability, it can be used to control the quality of Yankening tablets.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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