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Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province based on UPLC-Q-Orbitrap HRMS
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Yi-jun SONG1, 2, Tao GUO3, Yu-ying LIANG1, Geng-chen GUAN1, Yang-li GAO1
Chinese Journal of Pharmaceutical Analysis | 2024, 44(8) : 1293 - 1302
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Chinese Journal of Pharmaceutical Analysis | 2024, 44(8): 1293-1302
• Ingredient Analys •
Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province based on UPLC-Q-Orbitrap HRMS
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Yi-jun SONG1, 2, Tao GUO3, Yu-ying LIANG1, Geng-chen GUAN1, Yang-li GAO1
Affiliations
  • 1.College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，Chinia
  • 2.Shaanxi Engineering Technology Research Center of Prepared Slices，Xianyang 712046，China
  • 3.No.986 Hospital of PLAAF，Xi’an 710054，China
Published: 2024-08-31 doi: 10.16155/j.0254-1793.2024-0015
Outline
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Objective:

To analyze the difference of chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province.

Methods:

Ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS) was developed to determine the chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province. The data were analyzed by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The structures of chemical markers in rhizomes and leaves of Polygonatum sibiricum were identified based on accurate primary mass spectrometry and secondary mass spectrometry fragment ion，combined with the reference map，software database searching and related literature.

Results:

A total of 45 compounds were identified，there were 26 chemical ingredients with significant differences distinguished by the method of OPLS-DA，including 10 amino acids，6 flavonoids，3 organic acids，2 saccharides，1coumarin and 4 alkaloids.

Conclusion:

The chemical markers of amino acids，organic acids and alkaloids are mainly distributed in rhizomes，and the chemical markers of flavonoids are mainly concentrated in leaves part of the plant. It is suggested that the potential multiple utilization of rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province.

Polygonati Rhizoma  /  rhizomes  /  leaves  /  plant metabolomics  /  potential marker  /  principal component analysis (PCA)  /  orthogonal partial least squares discriminant analysis (OPLS-DA)  /  UPLC-Q-Orbitrap HRMS
Yi-jun SONG, Tao GUO, Yu-ying LIANG, Geng-chen GUAN, Yang-li GAO. Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province based on UPLC-Q-Orbitrap HRMS[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44 (8) : 1293 -1302 . DOI: 10.16155/j.0254-1793.2024-0015
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Year 2024 volume 44 Issue 8
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Article Info
doi: 10.16155/j.0254-1793.2024-0015
  • Receive Date：2024-01-09
  • Online Date：2026-03-13
  • Published：2024-08-31
Article Data
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History
  • Received：2024-01-09
Funding
Affiliations
    1.College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，Chinia
    2.Shaanxi Engineering Technology Research Center of Prepared Slices，Xianyang 712046，China
    3.No.986 Hospital of PLAAF，Xi’an 710054，China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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