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To establish an integrated model of biosensing technology for the study of zebrafish，and to study and verify the interaction between cetirizine hydrochloride，fexofenadine hydrochloride，mizolastine，and ebastine，with the key target of allergic rhinitis，macrophage migration inhibitory factor（MIF）.
A MIF-high electron mobility transistor(HEMT) biosensor was constructed using AlGaAs/GaAs HEMT as the sensing element and MIF as the biological element. The interaction strength between cetirizine hydrochloride，fexofenadine hydrochloride，mizolastine，and ebastine and MIF was characterized. Furthermore，a zebrafish immune inflammation model was established. The number of neutrophil migration in the blank control group，model group，positive drug group，and low，medium，and high-dose drug groups were recorded. And the drug inflammation inhibition rate were calculated.
Cetirizine hydrochloride exhibited the strongest binding ability to MIF，with a dissociation constant of 7.05×10-13 mol·L-1. The KD for the interactions between fexofenadine hydrochloride，mizolastine，and ebastine with MIF were 2.34×10-8 mol·L-1，1.94×10-7 mol·L-1 and 2.44×10-8 mol·L-1，respectively. All of them had binding effects with MIF. Further the validation using the zebrafish immune anti-inflammatory model revealed that all four antihistamines significantly reduced the number of neutrophil migrations，among which 100 μmol·L-1 of cetirizine hydrochloride achieved a neutrophil migration inhibition rate of 68.5%.
This study explores the interaction between antihistamines and the key protein target of AR through the integration of biosensing technology and zebrafish experiments. It was found that MIF is a potential target for antihistamine drugs to exert their pharmacological effects. It provides an important reference for research on drug efficacy and its association with targets.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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