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Identification of novel anti-depressant compound WJ-14 metabolites in rats and human liver microsomes by HPLC-MS/MS*
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Wei-yin HUANG1, Li-shou YANG2, 3, Yi-wen ZHANG4, Xiao-sheng YANG2, 3, **, Lin-hu YE1, 5, **
Chinese Journal of Pharmaceutical Analysis | 2024, 44(12) : 2023 - 2032
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Chinese Journal of Pharmaceutical Analysis | 2024, 44(12): 2023-2032
• Metabolism Analysis •
Identification of novel anti-depressant compound WJ-14 metabolites in rats and human liver microsomes by HPLC-MS/MS*
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Wei-yin HUANG1, Li-shou YANG2, 3, Yi-wen ZHANG4, Xiao-sheng YANG2, 3, **, Lin-hu YE1, 5, **
Affiliations
  • 1.Department of Pharmacy, Zhejiang Provincial People’s Hospital Bijie Hospital, Bijie 551700, China
  • 2.Natural Products Research Center of Guizhou Province, Guiyang 550014, China
  • 3.State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550025, China
  • 4.Department of Pharmacy, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou 310014, China
  • 5.The Sixth Affiliated Hospital of South China University of Technology, Foshan 528000, China
Published: 2024-12-31 doi: 10.16155/j.0254-1793.2024-0276
Outline
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Objective:

To investigate the metabolites and metabolic pathways of WJ-14, a novel anti-depressant compound, in rats and human liver microsomes by HPLC-MS/MS.

Methods:

Samples of rat plasma and urine, as well as in vitro incubation system of human liver microsomes were analyzed by HPLC-MS/MS. Chromatographic conditions: the chromatographic conditions were as follows: Hypersil BDS C18 (100 mm×4.6 mm, 3 μm) column, with 0.1% formic acid aqueous solution (A)-methanol (B) as the mobile phase, gradient elution flow rate 0.4 mL·min-1, column temperature 40 ℃, and injection volume 5 μL. The identification of metabolites and speculation on the metabolic pathways of WJ-14 were conducted by comparing the fragmentation mode and characteristic fragment ions of WJ-14.

Results:

A total of 12 metabolites of WJ-14 were identified, including 6 in rat and 6 in human liver microsomes, with two metabolites were detected in both rat and human liver microsomes. The major metabolic pathways of WJ-14 were identified as hydroxylation, demethylation, hydration, glucuronidation and methylation.

Conclusion:

In this work, we investigated the metabolites and metabolic pathways of WJ-14 using HPLC-MS/MS for the first time. The metabolism of WJ-14 occurred in phase Ⅰ and phase Ⅱ metabolic reactions, in which hydroxylation in phase Ⅰ is dominant. The results of the current study may provide valuable information for the formulation development and application of WJ-14.

WJ-14  /  HPLC-MS/MS  /  metabolites  /  human liver microsomes  /  metabolic pathways
Wei-yin HUANG, Li-shou YANG, Yi-wen ZHANG, Xiao-sheng YANG, Lin-hu YE. Identification of novel anti-depressant compound WJ-14 metabolites in rats and human liver microsomes by HPLC-MS/MS*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44 (12) : 2023 -2032 . DOI: 10.16155/j.0254-1793.2024-0276
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Year 2024 volume 44 Issue 12
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Article Info
doi: 10.16155/j.0254-1793.2024-0276
  • Receive Date：2024-04-24
  • Online Date：2026-03-13
  • Published：2024-12-31
Article Data
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History
  • Received：2024-04-24
Funding
Affiliations
    1.Department of Pharmacy, Zhejiang Provincial People’s Hospital Bijie Hospital, Bijie 551700, China
    2.Natural Products Research Center of Guizhou Province, Guiyang 550014, China
    3.State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550025, China
    4.Department of Pharmacy, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou 310014, China
    5.The Sixth Affiliated Hospital of South China University of Technology, Foshan 528000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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