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To establish the fingerprint of Panax quinquefolius L. from Shandong by UPLC-PDA，and to simultaneously determine the contents of 16 ginsenosides.
The chromatographic column was an Acquity UPLC BEH C18 column(50 mm×2.1 mm，1.7 μm)，which was eluted with water-acetonitrile by gradient at a flow rate of 0.4 mL·min-1. The detection wavelength was 203 nm，the column temperature was 30 °C，and the injection volume was 2 μL. The Chinese Pharmacopoeia “Chinese Medicine Chromatography Fingerprint Similarity Evaluation System (2012 Edition)” was used for evaluation，and 42 batches of Panax quinquefolius L. from different habitats were compared with cluster analysis and principal component analysis.
The total content 16 ginsenosides in Panax quinquefolius L. from in Shandong were 19.73-58.07 mg·g-1，and the average value was (34.72±8.22) mg·g-1. The fingerprints of the ginsenosides in Panax quinquefolius L. from Shandong were established，and the similarities were above 0.90. And 10 common peaks constituted the characteristic peaks of Panax quinquefolius L.. Cluster analysis and principal component analysis showed that the contents of ginsenosides in Panax quinquefolius L. from Shandong were stable.
The fingerprint of Panax quinquefolius L. established is highly characteristic，and the method issimple，which provides data support for the identification and quality control of Panax quinquefolius L..
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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