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To establish the ultra high performance liquid chromatography (UPLC) fingerprint of Qiye Shen’an dropping pills，and to determine the contents of 8 index components by quantitative analysis of multi-components by single marker at the same time.
Waters CORTECS® T3 (150 mm×2.1 mm，1.6 μm) chromatography column was used for gradient elution with acetonitrile -0.1% phosphoric acid as mobile phase，flow rate was 0.40 mL·min-1，column temperature was 35 ℃，detection wavelength was 203 nm.
The UPLC fingerprint of Qiye Shen’an dropping pills was established，8 common peaks were established，and the similarity of 15 batches of samples was greater than 0.99. Ginsenoside Rb1，ginsenoside RC，panax notoginseng saponin FC，ginsenoside Rb2，ginsenoside Rb3，ginsenoside Rd，panax notoginseng saponin Fe and panax notoginseng saponin Fd were determined by quantitative analysis of multi-components by single marker method. The contents of 8 components in 15 batches were between 7.6-10.0 mg per pill. The average recoveries were 99.8%，100.6%，99.8%，101.2%，102.1%，101.9%，99.7% and 100.2% respectively，and the RSDs were 2.3%，2.2%，2.0%，1.5%，1.7%，1.2%，2.4% and 2.6% respectively.
The UPLC fingerprint of Qiye Shen’an dropping pills and the content determination method of quantitative analysis of multi-components by single marker method established in this study are simple to operation，and have good repeatability，stability and reliability，and can provide a more comprehensive basis for the quality control of Qiye Shen’an dropping pills.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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