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To establish a UHPLC method for simultaneously determining sinomenine，neochlorogenic acid，magnolflorine，cryptochlorogenic acid，chlorogenic acid，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C，harpagoside，ligustilide and ammonium glycyrrhizate in Jinteng Qingbi granules，and evaluate the quality of Jinteng Qingbi granules combined with principal component analysis.
UHPLC wavelength switching method was employed in the study. The chromatographic separation was performed on a Waters XSelect® CSH C18 column (150 mm×4.6 mm，2.5 μm) using methanol-acetonitrile (1:1) as mobile phase A and 0.2% phosphoric acid aqueous solution as mobile phase B in a gradient mode at 25 ℃. The flow rate was 1.0 mL·min-1，and the UV detection wavelength was chosen at 218 nm for sinomenine during 0-17 min，326 nm for neochlorogenic acid，cryptochlorogenic acid，chlorogenic acid，isochlorogenic acid B，isochlorogenic acid A and isochlorogenic acid C during 17-25 min and 34-98nm，263 nm for magnolflorine，harpagoside，ligustilide and ammonium glycyrrhizate during 25-34 min and 98-125nm，respectively. Furthermore，multiple statistical analysis was conducted on the contents of 11 components in 20 batches of Jinteng Qingbi granules using SPSS27.0 software.
Satisfactory linearities of sinomenine，neochlorogenic acid，magnolflorine，cryptochlorogenic acid，chlorogenic acid，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C，harpagoside，ligustilide and ammonium glycyrrhizate were in the ranges of 14.36-143.61 μg·mL-1 (r=0.999 9)，7.71-77.15 μg·mL-1 (r=0.999 8)，9.18-91.83 μg·mL-1 (r=0.999 7)，10.71-107.07 μg·mL-1 (r=0.999 8)，12.88-128.80 μg·mL-1 (r=0.999 8)，5.20-51.95 μg·mL-1 (r=0.999 7)，5.18-51.84 μg·mL-1 (r=0.999 8)，5.40-53.95 μg·mL-1 (r=0.999 8)，2.62-26.16 μg·mL-1 (r=0.999 9)，6.31-63.06 μg·mL-1 (r=0.999 9) and 11.13-111.26 μg·mL-1 (r=0.997 6)，respectively. The average recoveries (n=6) were 98.3%，98.3%，98.5%，98.9%，99.2%，101.0%，98.1%，97.1%，96.8%，98.0% and 98.7%，respectively，with RSDs less than 3.0%. The contents ranges of sinomenine and other 10 components of 20 batches of Jinteng Qingbi granules samples were 2.206-2.704，1.071-1.403，2.096-2.487，1.321-1.724，2.241-2.612，0.605-0.749，0.363-0.412，0.835-1.020，0.151-0.191，0.791-1.188 and 1.008-1.363 mg·g-1，respectively. The results of principal component analysis showed that the quality differences between batches of continuously produced Jinteng Qingbi granules were relatively smaller，and the comprehensive quality of samples S1，S5 and S7 was relatively better.
The established UHPLC method of multi-index component determination is simple，accurate and stable. Combined with principal component analysis，it can be used for quality evaluation of Jinteng Qingbi granules comprehensively.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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