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Live-cell panoramic super-resolution microscopy: techniques and applications
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Jiahui Liu1, 2, Yanqing Yang3, 4, Fei Chen3, Yanli Li3, Yijiang Wang3, Qiukai Feng3, Yuling Qin1, *, kebin Shi3, 5
Modern Instruments & Medical Treatment | 2025, 31(4) : 67 - 72
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Modern Instruments & Medical Treatment | 2025, 31(4): 67-72
Live-cell panoramic super-resolution microscopy: techniques and applications
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Jiahui Liu1, 2, Yanqing Yang3, 4, Fei Chen3, Yanli Li3, Yijiang Wang3, Qiukai Feng3, Yuling Qin1, *, kebin Shi3, 5
Affiliations
  • 1.School of Public Health, Nantong University, Nantong 226000, China
  • 2.Laboratory Department, Nantong Integrated Traditional Chinese and Western Medicine Hospital, Nantong 226000, China
  • 3.Peking University Yangtze Delta Institute of Optoelectronics, Nantong 226000, China
  • 4.Pellucid Optics Technology (Nantong) Co., Ltd., Nantong 226000, China
  • 5.State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China
Published: 2025-08-10 doi: 10.11876/mimt202504007
Outline
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Objective

Based on live-cell panoramic super-resolution microscopy, complemented by AI-based image segmentation algorithms, we are developing methods for dynamically observing the entire life cycle of living cells.

Methods

Using COS-7 as a model, the mitotic process, subcellular organelle interactions, and intercellular communication were dynamically observed in panorama through live-cell panoramic super-resolution microscopy. Optical diffraction tomography (ODT) was employed to enable dynamic tracking of subcellular organelles in living cells, while spinning disk confocal microscopy provided molecular-level specificity validation. Furthermore, AI-based segmentation algorithms were implemented to accomplish qualitative segmentation and quantitative analysis of subcellular organelles.

Results

The system avoids or mitigates phototoxicity, photobleaching, and other artificial interference associated with traditional fluorescence imaging, enabling native-state dynamic observation of living cells.

Conclusion

The dual-modality joint imaging establishes a “structure-function complementarity” research paradigm. This innovative fusion strategy not only overcomes the limitations of traditional single-modality imaging techniques but also provides a novel multidimensional dynamic observation approach for investigating cell-cell interactions and organelle-organelle interaction mechanisms.

Label-free imaging  /  Super-resolution imaging  /  Spinning-disk confocal microscopy  /  Data segmentation  /  Artificial intelligence
刘嘉慧, 杨燕青, 陈飞, 李艳莉, 王艺江, 冯邱锴, 秦玉岭, 施可彬. 活细胞全景超分辨显微成像技术与应用. 现代仪器与医疗, 2025 , 31 (4) : 67 -72 . DOI: 10.11876/mimt202504007
Jiahui Liu, Yanqing Yang, Fei Chen, Yanli Li, Yijiang Wang, Qiukai Feng, Yuling Qin, kebin Shi. Live-cell panoramic super-resolution microscopy: techniques and applications[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 67 -72 . DOI: 10.11876/mimt202504007
Appendix
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Year 2025 volume 31 Issue 4
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doi: 10.11876/mimt202504007
  • Online Date：2026-10-23
  • Published：2025-08-10
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Affiliations
    1.School of Public Health, Nantong University, Nantong 226000, China
    2.Laboratory Department, Nantong Integrated Traditional Chinese and Western Medicine Hospital, Nantong 226000, China
    3.Peking University Yangtze Delta Institute of Optoelectronics, Nantong 226000, China
    4.Pellucid Optics Technology (Nantong) Co., Ltd., Nantong 226000, China
    5.State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China
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小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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