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Research progress of intravital imaging
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Fangfei Yu1, 2, Zhi Lu1, 3, Shaowei Jiang2, Yi Yang1, 4, 5, 6, *
Modern Instruments & Medical Treatment | 2025, 31(4) : 73 - 80
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Modern Instruments & Medical Treatment | 2025, 31(4): 73-80
Research progress of intravital imaging
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Fangfei Yu1, 2, Zhi Lu1, 3, Shaowei Jiang2, Yi Yang1, 4, 5, 6, *
Affiliations
  • 1.Zhejiang Hehu Technology Co., Ltd., Hangzhou 311113, China
  • 2.School of Communication Engineering, Hangzhou Dianzi University, Hangzhou 310018, China
  • 3.Department of Automation, Tsinghua University, Beijing 100084, China
  • 4.School of Reliability and Systems Engineering, Beihang University, Beijing 100191, China
  • 5.Peng Cheng Laboratory, Shenzhen 518000, China
  • 6.Hangzhou Innovation Institute of Beihang University, Hangzhou 310052, China
Published: 2025-08-10 doi: 10.11876/mimt202504008
Outline
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As a pivotal tool in life science research, intravital microscopy facilitates real-time monitoring of cellular and molecular dynamics within their native physiological milieu. This review delves into the imaging mechanisms, strengths, and constraints of confocal microscopy, multi-photon microscopy, light-sheet microscopy, and scanning light-field microscopy. By analyzing representative application cases across disciplines such as oncology, immunology, neuroscience, and developmental biology, it offers a comparative overview of the ideal usage scenarios for these technologies. Furthermore, it addresses current technical hurdles and speculates on future advancements in the field. To provide technical reference for related research.

Intravital imaging  /  Confocal microscopy  /  Multi-photon microscopy  /  Light-sheet microscopy  /  Scanning light-field microscopy
俞方菲, 卢志, 江劭玮, 杨懿. 活体显微成像技术研究进展. 现代仪器与医疗, 2025 , 31 (4) : 73 -80 . DOI: 10.11876/mimt202504008
Fangfei Yu, Zhi Lu, Shaowei Jiang, Yi Yang. Research progress of intravital imaging[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 73 -80 . DOI: 10.11876/mimt202504008
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Year 2025 volume 31 Issue 4
68
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doi: 10.11876/mimt202504008
  • Online Date：2026-10-23
  • Published：2025-08-10
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Affiliations
    1.Zhejiang Hehu Technology Co., Ltd., Hangzhou 311113, China
    2.School of Communication Engineering, Hangzhou Dianzi University, Hangzhou 310018, China
    3.Department of Automation, Tsinghua University, Beijing 100084, China
    4.School of Reliability and Systems Engineering, Beihang University, Beijing 100191, China
    5.Peng Cheng Laboratory, Shenzhen 518000, China
    6.Hangzhou Innovation Institute of Beihang University, Hangzhou 310052, China
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https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/10.11876/mimt202504008
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多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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