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Polarization control and applications in structured illumination microscopy
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Wenyi Wang1, Peng Xi1, 2, Meiqi Li3, Liang Qiao1, *
Modern Instruments & Medical Treatment | 2025, 31(4) : 58 - 66
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Modern Instruments & Medical Treatment | 2025, 31(4): 58-66
Polarization control and applications in structured illumination microscopy
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Wenyi Wang1, Peng Xi1, 2, Meiqi Li3, Liang Qiao1, *
Affiliations
  • 1.Airy Technologies Co., Ltd., Beijing 100086, China
  • 2.College of Future Technology, Peking University, Beijing 100871, China
  • 3.School of Life Sciences, Peking University, Beijing 100871, China
Published: 2025-08-10 doi: 10.11876/mimt202504006
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Fluorescence polarization analysis requires rotating the excitation beam's polarization direction, while conventional structured illumination microscopy (SIM) relies on three illumination orientations to achieve super-resolution. Polarized structured illumination microscopy (Polar-SIM), as an integrated approach combining these two techniques, enables high-precision investigation of the spatial orientation and structure of various subcellular organelles. This study theoretically investigates the relationship between the contrast of interferometric fringes and polarization modulation, compares the advantages and disadvantages of employing a pizza waveplate, circular polarization, and a variable liquid crystal phase retarder for polarization modulation, and further simulates the accuracy of polarization analysis under different modulation conditions. Finally, our experimental results demonstrate Polar-SIM's unique capability for super-resolution directional analysis of molecular dipoles, highlighting its broad potential for addressing diverse biological questions in future research.

Structured illumination microscopy  /  Fluorescence polarization microscopy  /  Dipole orientation analysis  /  Super-resolution fluorescence imaging
王文熠, 席鹏, 李美琪, 乔良. 结构光照明显微成像系统中的偏振控制及成像应用. 现代仪器与医疗, 2025 , 31 (4) : 58 -66 . DOI: 10.11876/mimt202504006
Wenyi Wang, Peng Xi, Meiqi Li, Liang Qiao. Polarization control and applications in structured illumination microscopy[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 58 -66 . DOI: 10.11876/mimt202504006
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Year 2025 volume 31 Issue 4
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doi: 10.11876/mimt202504006
  • Online Date：2026-10-23
  • Published：2025-08-10
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    1.Airy Technologies Co., Ltd., Beijing 100086, China
    2.College of Future Technology, Peking University, Beijing 100871, China
    3.School of Life Sciences, Peking University, Beijing 100871, China
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