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Full-chain solution for single-molecule localization super-resolution microscopy
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Fen Hu1, 2, Bo Wang1, Zhao Xie1, Jiqiang Li1, Mingxin Chen1, Shuai Liu1, Jianyu Yang1, Imshik Lee3, Leiting Pan1, 2, 4, *
Modern Instruments & Medical Treatment | 2025, 31(4) : 45 - 57
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Modern Instruments & Medical Treatment | 2025, 31(4): 45-57
Full-chain solution for single-molecule localization super-resolution microscopy
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Fen Hu1, 2, Bo Wang1, Zhao Xie1, Jiqiang Li1, Mingxin Chen1, Shuai Liu1, Jianyu Yang1, Imshik Lee3, Leiting Pan1, 2, 4, *
Affiliations
  • 1.Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China
  • 2.Tianjin Nano-Microimaging Biotechnology Co., Ltd., Tianjin 300192, China
  • 3.Medical School, Central Asian University, Tashkent 111221, Uzbekistan
  • 4.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
Published: 2025-08-10 doi: 10.11876/mimt202504005
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Single-molecule localization microscopy (SMLM), as a groundbreaking technology surpassing the optical diffraction limit, achieves nanoscale spatial resolution (～20 nm) and has revolutionized biological imaging. However, its widespread application faces critical challenges including reliance on imported hardware systems, complex sample preparation protocols, lack of dual-color imaging fluorescent probes, and insufficient data analysis and mining, leading to underutilization of existing equipment. This review proposes a comprehensive full-chain solution addressing nine critical elements of SMLM, which include three “hard skills” — hardware systems, software control, and image reconstruction; and six “soft skills” — precise sample preparation, blinking probes, data analysis and mining, specialized reagents and consumables, specific antibodies, and practical applications. The “hard skills” can be rapidly deployed through the procurement and integration of mature commercial components, whereas the “soft skills” rely on long-term technological accumulation and deep domain knowledge. This solution aims to promote the transformation of SMLM from a “high-end instrument” into an “efficient tool”, facilitating the broader application of super-resolution imaging technologies and offering new perspectives for the use of advanced microscopy systems.

Super-resolution microscopy  /  Single-molecule localization  /  Full-chain solution  /  Automated sample preparation
胡芬, 王波, 解钊, 黎继强, 陈明鑫, 刘帅, 杨建宇, 李任植, 潘雷霆. 单分子定位超分辨成像全链条解决方案. 现代仪器与医疗, 2025 , 31 (4) : 45 -57 . DOI: 10.11876/mimt202504005
Fen Hu, Bo Wang, Zhao Xie, Jiqiang Li, Mingxin Chen, Shuai Liu, Jianyu Yang, Imshik Lee, Leiting Pan. Full-chain solution for single-molecule localization super-resolution microscopy[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 45 -57 . DOI: 10.11876/mimt202504005
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Year 2025 volume 31 Issue 4
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doi: 10.11876/mimt202504005
  • Online Date：2026-10-23
  • Published：2025-08-10
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Affiliations
    1.Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China
    2.Tianjin Nano-Microimaging Biotechnology Co., Ltd., Tianjin 300192, China
    3.Medical School, Central Asian University, Tashkent 111221, Uzbekistan
    4.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
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