Full
Outline
With the development of single-cell sequencing technology, life science research has gradually moved from population average measurement to cell resolution, which provides a new research method for understanding the heterogeneity and cell state changes within tissues. The continuous progress of this technology system is not only due to the improvement of sequencing chemistry, but also closely related to the continuous optimization of instrument platforms such as microfluidic droplets, molecular barcoding, long-read sequencing and spatially resolved detection. Different technical routes have differences in sequencing throughput, read length characteristics and noise structure, which largely affect the subsequent data processing methods and promote the continuous adjustment and development of bioinformatics analysis methods. Combined with the main engineering paths of the single-cell sequencing instrument platform, this paper combs the representative solutions such as physical sorting, microfluidic system, long-read sequencing and space technology. At the same time, we discuss the characteristics of data structure shaped by barcodes and unique molecular identifiers (UMI), the statistical modeling problem caused by high sparsity, and the requirements of computational framework for large-scale data analysis. In addition, the development trend of single-cell sequencing and bioinformatics platforms in automation, multimodal integration and computing power improvement are summarized, in order to provide reference for the optimization of related technical systems.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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