Full
Outline
To transfer and verify the reference interval for serum lithium determination by inductively coupled plasma mass spectrometry (ICP-MS), and to evaluate the consistency of this method with the ion-selective electrode (ISE).
A total of 450 serum samples from patients treated with lithium carbonate were collected and detected in parallel by ICP-MS and ISE methods. Among them, 150 samples were used for reference interval transfer of ICP-MS, 100 samples for reference interval verification, and 200 samples for evaluating the consistency between ICP-MS and ISE methods in serum lithium detection.
Reference interval transfer showed a high correlation between ICP-MS and ISE methods for serum lithium detection (r=0.980, P<0.01), with the regression equation ŶICP-MS=0.060+0.964XISE and R2=0.976. The ratio of the absolute value of the intercept to the width of the ISE reference interval was 6%, and the transferred reference interval of ICP-MS was 0.25~1.22 mmol/L. The verification pass rate was 98%. Consistency analysis indicated that the detection values of ICP-MS were generally lower than those of ISE (P<0.05); in the low concentration group, the detection values of ICP-MS were significantly higher (P<0.05), while in the therapeutic concentration Ⅱ group and high concentration group, the detection values of ICP-MS were significantly lower (P<0.05). The two methods showed an overall positive correlation (r=0.961, P<0.01) with R2=0.924, and the proportion of points outside the 95% limits of agreement (LoA) was 2.5%. The linear bias was 0.000 4±0.190 0, and the absolute bias met the criterion (≤±0.3 mmol/L) for total allowable error (TEa). In subgroup analysis, the correlation coefficients of each group ranged from 0.737 to 0.870, R2 from 0.543 to 0.927, and the proportion of points outside the 95% LoA ranged from 2.9% to 8.3%. The linear bias of each group ranged from -0.000 4±0.069 0 to 0.001 0±0.099 0, and the absolute bias in all groups met the TEa requirement.
The ICP-MS method is reliable for serum lithium detection and shows good consistency with the ISE method, which can serve as an effective alternative for clinical serum lithium determination.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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