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Outline
Fluorescence polarization analysis requires rotating the excitation beam's polarization direction, while conventional structured illumination microscopy (SIM) relies on three illumination orientations to achieve super-resolution. Polarized structured illumination microscopy (Polar-SIM), as an integrated approach combining these two techniques, enables high-precision investigation of the spatial orientation and structure of various subcellular organelles. This study theoretically investigates the relationship between the contrast of interferometric fringes and polarization modulation, compares the advantages and disadvantages of employing a pizza waveplate, circular polarization, and a variable liquid crystal phase retarder for polarization modulation, and further simulates the accuracy of polarization analysis under different modulation conditions. Finally, our experimental results demonstrate Polar-SIM's unique capability for super-resolution directional analysis of molecular dipoles, highlighting its broad potential for addressing diverse biological questions in future research.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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