Home Journals Articles Updates About
CN
image
收藏切换
Full-chain solution for single-molecule localization super-resolution microscopy
收藏切换
Fen Hu1, 2, Bo Wang1, Zhao Xie1, Jiqiang Li1, Mingxin Chen1, Shuai Liu1, Jianyu Yang1, Imshik Lee3, Leiting Pan1, 2, 4, *
Modern Instruments & Medical Treatment | 2025, 31(4) : 45 - 57
Less
收藏切换
Modern Instruments & Medical Treatment | 2025, 31(4): 45-57
Full-chain solution for single-molecule localization super-resolution microscopy
Full
Fen Hu1, 2, Bo Wang1, Zhao Xie1, Jiqiang Li1, Mingxin Chen1, Shuai Liu1, Jianyu Yang1, Imshik Lee3, Leiting Pan1, 2, 4, *
Affiliations
  • 1.Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China
  • 2.Tianjin Nano-Microimaging Biotechnology Co., Ltd., Tianjin 300192, China
  • 3.Medical School, Central Asian University, Tashkent 111221, Uzbekistan
  • 4.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
Published: 2025-08-10 doi: 10.11876/mimt202504005
Outline
收藏切换

Single-molecule localization microscopy (SMLM), as a groundbreaking technology surpassing the optical diffraction limit, achieves nanoscale spatial resolution (～20 nm) and has revolutionized biological imaging. However, its widespread application faces critical challenges including reliance on imported hardware systems, complex sample preparation protocols, lack of dual-color imaging fluorescent probes, and insufficient data analysis and mining, leading to underutilization of existing equipment. This review proposes a comprehensive full-chain solution addressing nine critical elements of SMLM, which include three “hard skills” — hardware systems, software control, and image reconstruction; and six “soft skills” — precise sample preparation, blinking probes, data analysis and mining, specialized reagents and consumables, specific antibodies, and practical applications. The “hard skills” can be rapidly deployed through the procurement and integration of mature commercial components, whereas the “soft skills” rely on long-term technological accumulation and deep domain knowledge. This solution aims to promote the transformation of SMLM from a “high-end instrument” into an “efficient tool”, facilitating the broader application of super-resolution imaging technologies and offering new perspectives for the use of advanced microscopy systems.

Super-resolution microscopy  /  Single-molecule localization  /  Full-chain solution  /  Automated sample preparation
胡芬, 王波, 解钊, 黎继强, 陈明鑫, 刘帅, 杨建宇, 李任植, 潘雷霆. 单分子定位超分辨成像全链条解决方案. 现代仪器与医疗, 2025 , 31 (4) : 45 -57 . DOI: 10.11876/mimt202504005
Fen Hu, Bo Wang, Zhao Xie, Jiqiang Li, Mingxin Chen, Shuai Liu, Jianyu Yang, Imshik Lee, Leiting Pan. Full-chain solution for single-molecule localization super-resolution microscopy[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 45 -57 . DOI: 10.11876/mimt202504005
Appendix
Less
Year 2025 volume 31 Issue 4
59
12
Cite this Article
BibTeX
Article Info
doi: 10.11876/mimt202504005
  • Online Date：2026-10-23
  • Published：2025-08-10
Article Data
Affiliations
History
Funding
Affiliations
    1.Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, China
    2.Tianjin Nano-Microimaging Biotechnology Co., Ltd., Tianjin 300192, China
    3.Medical School, Central Asian University, Tashkent 111221, Uzbekistan
    4.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
References
Share
https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/10.11876/mimt202504005
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House