Full
Outline
This paper first compares point scanning confocal microscopy with spinning disk confocal microscopy in fluorescence confocal microscopy. The former is a serial imaging with single point excitation-detection and has a slower speed; the latter is a parallel imaging with multi-point excitation-detection, which is fast. Furthermore, by comparing the technical principles of single-disk and dual-disk confocal focusing, the limited pinhole size, spacing, and distance between the two disks caused by the micro lens disk in the dual-disk setup were revealed, resulting in limited field-of-view, imaging quality, rotation speed, and the need for precise registration of the disk system. The technical characteristics of ceiling-less single disk confocal system were explained, including multi-color, fast (fast rotation speed, large field-of-view), easy-to-use (high optical quality, customizable pinhole size and spacing), stabilization (high stability, low cost), and its performance was demonstrated through resolution testing (xy direction resolution 230 nm, z direction resolution 470 nm). Finally, the characteristics of the three technologies in terms of resolution and penetration depth, photobleaching and phototoxicity, signal-to-noise ratio, imaging rate, system complexity and imaging field of view were compared. The comparison showed that the single-disk technology outperforms the point scanning and dual-disk technologies in terms of imaging field of view, imaging rate, phototoxicity and system complexity, thus demonstrating its outstanding application value in biomedical research.
80
13
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
