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Research progress on multiscale, high-speed, and low-phototoxicity light sheet microscopy
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Yifan Ma1, Yuxuan Zhao1, Fang Zhao1, Meiling Chu2, Peng Fei1, *
Modern Instruments & Medical Treatment | 2025, 31(4) : 16 - 21
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Modern Instruments & Medical Treatment | 2025, 31(4): 16-21
Research progress on multiscale, high-speed, and low-phototoxicity light sheet microscopy
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Yifan Ma1, Yuxuan Zhao1, Fang Zhao1, Meiling Chu2, Peng Fei1, *
Affiliations
  • 1.School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, China
  • 2.Wuhan Smartview Biotechnology Co., Ltd., Wuhan 430000, China
Published: 2025-08-10 doi: 10.11876/mimt202504002
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Fluorescence microscopy has become an indispensable tool for visualizing subcellular structures and dynamic biological processes. Light sheet fluorescence microscopy (LSFM), characterized by selective plane illumination and detection, has attracted increasing attention in recent years owing to its low phototoxicity, high spatiotemporal resolution, and large field of view. The emergence of single-objective light sheet systems has further enhanced sample compatibility and system flexibility, opening new avenues for multiscale, long-term, and three-dimensional dynamic imaging. Here, we review the development and diverse configurations of LSFM, with a particular focus on the technical principles, representative applications, and performance advantages of single-objective systems. We also discuss their current bottlenecks in terms of field of view, optical path complexity, and signal transmission efficiency, and explore the potential of zoom-free multiscale single-objective platforms that integrate structural innovations, multimodal imaging, and intelligent control, aiming to provide a reference for the creation of high-performance, bio-friendly, and broadly applicable imaging systems.

Light sheet fluorescence microscopy  /  Single-objective light sheet systems  /  Low phototoxicity  /  Multi-scale imaging  /  3D imaging
马异凡, 赵宇轩, 赵方, 储美玲, 费鹏. 多尺度、高速、低光毒性光片荧光显微技术研究进展. 现代仪器与医疗, 2025 , 31 (4) : 16 -21 . DOI: 10.11876/mimt202504002
Yifan Ma, Yuxuan Zhao, Fang Zhao, Meiling Chu, Peng Fei. Research progress on multiscale, high-speed, and low-phototoxicity light sheet microscopy[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 16 -21 . DOI: 10.11876/mimt202504002
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Year 2025 volume 31 Issue 4
81
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doi: 10.11876/mimt202504002
  • Online Date：2026-10-23
  • Published：2025-08-10
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    1.School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, China
    2.Wuhan Smartview Biotechnology Co., Ltd., Wuhan 430000, China
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https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/10.11876/mimt202504002
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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