首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1241675631495278635, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241675628051755031, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202404454, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1714060800000, receivedDateStr=2024-04-26, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1773968139765, onlineDateStr=2026-03-20, pubDate=1732464000000, pubDateStr=2024-11-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1773968139765, onlineIssueDateStr=2026-03-20, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1773968139765, creator=13701087609, updateTime=1773968139765, updator=13701087609, issue=Issue{id=1241675628051755031, tenantId=1146029695717560320, journalId=1227665162245664772, year='2024', volume='51', issue='22', pageStart='4033', pageEnd='4224', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1773968138945, creator=13701087609, updateTime=1773968595676, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1241677543783322543, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241675628051755031, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1241677543783322544, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241675628051755031, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=4080, endPage=4085, ext={EN=ArticleExt(id=1241675631830822968, articleId=1241675631495278635, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Antimicrobial resistance, virulence profile, and molecular characterization of Listeriamonocytogenes isolated from ready-to-eat food, Guangxi, 2011-2020, columnId=1228016572783063333, journalTitle=Modern Preventive Medicine, columnName=Nutrition and Food Hygiene, runingTitle=null, highlight=null, articleAbstract=
Objective

To comprehensively analyze the occurrence, virulence characteristics, and molecular typing of Listeria monocytogenes (L.monocytogenes) isolated from ready-to-eat (RTE) food in Guangxi. By investigating the genetic attributes, antibiotic resistance and potential health risks of L.monocytogenes, this research contributes to our understanding of this pathogen.

Methods

We analyzed 158 isolates of L. monocytogenes from RTE foods collected in Guangxi between 2011 and 2020. Whole genome sequencing (WGS) was performed on all isolates and antimicrobial resistance were determined by broth microdilution.The study included the determination of sequence types (STs), serotype, serogroup, lineage, clonal complexes (CC), acquired resistance genes and the presence of L. monocytogenes pathogenicity islands (LIPIs).

Results

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi. The predominant serotypes were 1/2a, 3a.ST8/CC8 and ST87/CC87 were the dominant sequence types (STs) in RTE foods.All isolates were found to be susceptible to ampicillin(AMP), penicillin(PEN), tetracycline(TET), meropenem(MEM), and trimethoprim-sulfamethoxazole(SXT). Four isolates were resistant to ciprofloxacin. Two isolates wereresistant to erythromycin and vancomycin respectively. Acquired antimicrobial phenotypes were tet (M), msr (D) and mef (A) of an isolate resistant to erythromycin. Most of the isolates carried actA (79.74%), inlF (48.73%), inlK (55.69%)and all isolates carried other virulence genes.46.20%(73/158)of the isolatesharboured 12 virulence genes.It can be a potential public health risk due to its high harboring rate of 17 or 18 virulence genes.

Conclusion

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi.Acquired resistance genes and hypervirulence genes existed in this bacterium from RTE foods origins, and can be a major risk for consumers and public health.It was suggested that effective food safety measures should be implemented in RTE foods processing and service environments.

, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Xian-ying ZENG, Su-ling LV, Dong-mei TAN, Cong QU, Xiu-gui LI), CN=ArticleExt(id=1241675641662272186, articleId=1241675631495278635, tenantId=1146029695717560320, journalId=1227665162245664772, language=CN, title=2011—2020年广西即食食品中单增李斯特菌耐药性、毒力和分子特征研究, columnId=1228016573034721597, journalTitle=现代预防医学, columnName=营养与食品卫生, runingTitle=null, highlight=null, articleAbstract=
目的

分析广西即食食品中单核细胞增生李斯特菌（以下简称单增李斯特菌）的耐药性、分子特征和毒力特征，更好地了解即食食品中单增李斯特菌的遗传特点、耐药情况及其潜在的健康风险。

方法

采用肉汤稀释法对158株即食食品中单增李斯特进行药敏测定，同时对其进行全基因组测序（WGS），分析其序列ST型、血清群、血清型、CC型、谱系、耐药基因及毒力基因。

结果

2011—2020年广西即食食品中单增李斯特菌以1/2a,3a血清型为主，优势ST型为ST8/CC8和ST87/CC87。研究菌株均对AMP、PEN、TET、MEM、SXT敏感，4株分离株耐受CIP，2株单增李斯特菌分别耐受ERY和VAN。仅对红霉素耐药的1株分离株携带tet(M)、msr(D)、mef(A)耐药基因。actA、inlF、inlK的携带率分别为79.74%、48.73%、55.69%，其余毒力基因携带率为100%。46.20%（73/158）的菌株携带12个毒力基因，24株高毒力单增李斯特菌产生潜在高致病菌株。

结论

广西即食食品中单增李斯特菌存在的获得性耐药基因和高毒力基因对公众健康构成严重风险，应该对即食食品加工和服务环境采取有效的食品安全措施。

, correspAuthors=null, authorNote=null, correspAuthorsNote=
吕素玲，E-mail:
, copyrightStatement=本刊刊出的所有文章不代表中华预防医学会和本刊编委会的观点，除非特别声明。, copyrightOwner=中华预防医学会和四川大学华西公共卫生学院, extLink=null, articleAbsUrl=null, sourceXml=R5d6xSdklNQGFeNYk7Yvuw==, magXml=OUpX/O+iKQAUdJ9CWVnSOA==, pdfUrl=null, pdf=CR8KjXkRftOFOCcAW34Uug==, pdfFileSize=815879, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=R6T8el3zsb90kmw+be8bag==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=scuPec79H8jg8RP/507tAg==, mapNumber=null, authorCompany=null, fund=null, authors=

曾献莹（1989—），女，硕士，副主任技师，研究方向：食源性致病菌相关研究

, authorsList=曾献莹, 吕素玲, 谭冬梅, 瞿聪, 李秀桂)}, authors=[Author(id=1241826546856563518, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241826546978198342, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826546856563518, language=EN, stringName=Xian-ying ZENG, firstName=Xian-ying, middleName=null, lastName=ZENG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241826547078861644, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826546856563518, language=CN, stringName=曾献莹, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=广西壮族自治区疾病预防控制中心，广西　南宁　530028, bio={"content":"

曾献莹（1989—），女，硕士，副主任技师，研究方向：食源性致病菌相关研究

"}, bioImg=null, bioContent=

曾献莹（1989—），女，硕士，副主任技师，研究方向：食源性致病菌相关研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])]), Author(id=1241826547234050896, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=38662631@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241826547347297109, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826547234050896, language=EN, stringName=Su-ling LV, firstName=Su-ling, middleName=null, lastName=LV, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241826547460543324, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826547234050896, language=CN, stringName=吕素玲, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=广西壮族自治区疾病预防控制中心，广西　南宁　530028, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])]), Author(id=1241826547586372450, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241826547733173094, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826547586372450, language=EN, stringName=Dong-mei TAN, firstName=Dong-mei, middleName=null, lastName=TAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241826547896750957, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826547586372450, language=CN, stringName=谭冬梅, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=广西壮族自治区疾病预防控制中心，广西　南宁　530028, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])]), Author(id=1241826548001608563, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241826548135826297, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826548001608563, language=EN, stringName=Cong QU, firstName=Cong, middleName=null, lastName=QU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241826548253266815, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826548001608563, language=CN, stringName=瞿聪, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=广西壮族自治区疾病预防控制中心，广西　南宁　530028, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])]), Author(id=1241826548345541509, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241826548534285197, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826548345541509, language=EN, stringName=Xiu-gui LI, firstName=Xiu-gui, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241826548660114320, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, authorId=1241826548345541509, language=CN, stringName=李秀桂, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=广西壮族自治区疾病预防控制中心，广西　南宁　530028, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])])], keywords=[Keyword(id=1241826548899189655, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, orderNo=1, keyword=Listeria monocytogenes), Keyword(id=1241826549029213084, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, orderNo=2, keyword=Whole genome sequencing (WGS)), Keyword(id=1241826549146653597, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, orderNo=3, keyword=Antibiotic resistance), Keyword(id=1241826549251511203, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, orderNo=4, keyword=Virulence genes), Keyword(id=1241826549360563108, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, orderNo=1, keyword=单增李斯特菌), Keyword(id=1241826549478003625, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, orderNo=2, keyword=全基因组测序), Keyword(id=1241826549599638445, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, orderNo=3, keyword=抗生素耐药性), Keyword(id=1241826549691913136, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, orderNo=4, keyword=毒力基因)], refs=[Reference(id=1241826552921527258, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=21, issue=12, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［1］, rfOrder=0, authorNames=European Food Safety Authority, European Centre for Disease Prevention and Control, journalName=EFSA Journal. European Food Safety Authority, refType=null, unstructuredReference=European Food Safety Authority, European Centre for Disease Prevention and Control. The European Union one health 2022 zoonoses report［J］. EFSA Journal. European Food Safety Authority, 2023, 21(12): e8442., articleTitle=The European Union one health 2022 zoonoses report, refAbstract=null), Reference(id=1241826553001219035, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［2］, rfOrder=1, authorNames=World Health Organization, journalName=null, refType=null, unstructuredReference=World Health Organization. Listeriosis［EB/OL］. ［2024-09-19］. https://www.who.int/news-room/fact-sheets/detail/listeriosis., articleTitle=Listeriosis, refAbstract=null), Reference(id=1241826553189962718, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2024, volume=13, issue=1, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［3］, rfOrder=2, authorNames=Niu YL, Li WW, Xu BY, journalName=Emerging Microbes & Infections, refType=null, unstructuredReference=Niu YL, Li WW, Xu BY, et al. Risk factors associated with food consumption and food-handling habits for sporadic listeriosis: a case-control study in China from 2013 to 2022［J］. Emerging Microbes & Infections, 2024, 13(1): 2307520., articleTitle=Risk factors associated with food consumption and food-handling habits for sporadic listeriosis: a case-control study in China from 2013 to 2022, refAbstract=null), Reference(id=1241826553282237406, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2024, volume=13, issue=1, pageStart=57, pageEnd=null, url=null, language=null, rfNumber=［4］, rfOrder=3, authorNames=Tricoli MR, Massaro C, Arrigo I, journalName=Antibiotics, refType=null, unstructuredReference=Tricoli MR, Massaro C, Arrigo I, et al. Characterization of listeria monocytogenes strains isolated in palermo (Sicily and Italy) during the years 2018-2020 from severe cases of listeriosis［J］. Antibiotics, 2024, 13(1): 57., articleTitle=Characterization of listeria monocytogenes strains isolated in palermo (Sicily and Italy) during the years 2018-2020 from severe cases of listeriosis, refAbstract=null), Reference(id=1241826553412260831, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2021, volume=53, issue=1, pageStart=127, pageEnd=null, url=null, language=null, rfNumber=［5］, rfOrder=4, authorNames=Heidarlo MN, Lotfollahi L, Yousefi S, journalName=Tropical Animal Health and Production, refType=null, unstructuredReference=Heidarlo MN, Lotfollahi L, Yousefi S, et al. Analysis of virulence genes and molecular typing of Listeriamonocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran［J］. Tropical Animal Health and Production, 2021, 53(1): 127., articleTitle=Analysis of virulence genes and molecular typing of Listeriamonocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran, refAbstract=null), Reference(id=1241826553504535521, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［6］, rfOrder=5, authorNames=World Health Organization, journalName=null, refType=null, unstructuredReference=World Health Organization. Listeria monocytogenes in ready-to-eat (RTE) foods : Attribution, characterization and monitoring: Meeting report［EB/OL］. ［2024-09-19］. https://www.who.int/publications/i/item/9789240034969., articleTitle=Listeria monocytogenes in ready-to-eat (RTE) foods : Attribution, characterization and monitoring: Meeting report, refAbstract=null), Reference(id=1241826553613587427, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2018, volume=15, issue=8, pageStart=459, pageEnd=466, url=null, language=null, rfNumber=［7］, rfOrder=6, authorNames=Li WW, Bai L, Fu P, journalName=Foodborne Pathogens and Disease, refType=null, unstructuredReference=Li WW, Bai L, Fu P, et al. The epidemiology of listeria monocytogenes in China［J］. Foodborne Pathogens and Disease, 2018, 15(8): 459-466., articleTitle=The epidemiology of listeria monocytogenes in China, refAbstract=null), Reference(id=1241826553722639334, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2022, volume=12, issue=1, pageStart=3878, pageEnd=null, url=null, language=null, rfNumber=［8］, rfOrder=7, authorNames=Hassani S, Moosavy MH, Gharajalar SN, journalName=Scientific Reports, refType=null, unstructuredReference=Hassani S, Moosavy MH, Gharajalar SN, et al. High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk［J］. Scientific Reports, 2022, 12(1): 3878., articleTitle=High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk, refAbstract=null), Reference(id=1241826553844274152, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2024, volume=37, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［9］, rfOrder=8, authorNames=Moura A, Leclercq A, Vales G, journalName=The Lancet Regional Health. Europe, refType=null, unstructuredReference=Moura A, Leclercq A, Vales G, et al. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France［J］. The Lancet Regional Health. Europe, 2024, 37: 100800., articleTitle=Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France, refAbstract=null), Reference(id=1241826553957520361, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［10］, rfOrder=9, authorNames=Clinical and Laboratory Standards Institute, journalName=null, refType=null, unstructuredReference=Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd edition［EB/OL］. ［2024-09-19］. https://clsi.org/standards/products/microbiology/documents/m45/., articleTitle=Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd edition, refAbstract=null), Reference(id=1241826554053989355, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［11］, rfOrder=10, authorNames=European Committee on Antimicrobial Susceptibility Testing, journalName=null, refType=null, unstructuredReference=European Committee on Antimicrobial Susceptibility Testing. EUCAST: clinical breakpoints and dosing of antibiotics［EB/OL］. ［2024-09-19］. https://www.eucast.org/clinical_breakpoints., articleTitle=EUCAST: clinical breakpoints and dosing of antibiotics, refAbstract=null), Reference(id=1241826554129486828, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2020, volume=54, issue=2, pageStart=175, pageEnd=180, url=null, language=null, rfNumber=［12］, rfOrder=11, authorNames=李薇薇, 郭云昌, 占利, journalName=中华预防医学杂志, refType=null, unstructuredReference=李薇薇，郭云昌，占利，等．2017年中国即食食品中单核细胞增生李斯特菌的分子流行病学特征［J］．中华预防医学杂志, 2020, 54(2): 175-180., articleTitle=2017年中国即食食品中单核细胞增生李斯特菌的分子流行病学特征, refAbstract=null), Reference(id=1241826554200789997, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2020, volume=54, issue=2, pageStart=175, pageEnd=180, url=null, language=null, rfNumber=［12］, rfOrder=12, authorNames=Li WW, Guo YC, Zhan L, journalName=Chinese Journal of Preventive Medicine, refType=null, unstructuredReference=Li WW, Guo YC, Zhan L, et al. Molecular epidemiology of Listeria monocytogenes isolated from ready-to-eat food in 2017 in China［J］. Chinese Journal of Preventive Medicine, 2020, 54(2): 175-180. (In Chinese), articleTitle=Molecular epidemiology of Listeria monocytogenes isolated from ready-to-eat food in 2017 in China, refAbstract=null), Reference(id=1241826555698156526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2021, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［13］, rfOrder=13, authorNames=国家市场监督管理总局, 国家卫生健康委员会, journalName=null, refType=null, unstructuredReference=国家市场监督管理总局，国家卫生健康委员会．GB 31607-2021 食品安全国家标准 散装即食食品中致病菌限量［S］．北京：中国标准出版社，2021．, articleTitle=null, refAbstract=null), Reference(id=1241826555786236911, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2021, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［13］, rfOrder=14, authorNames=The State Administration for Market Regulation, the National Health Commission, journalName=null, refType=null, unstructuredReference=The State Administration for Market Regulation, the National Health Commission. GB 31607-2021 National standards for food safety-Pathogenic bacteria limit in bulk ready-to-eat food［S］. Beijing: Standards Press of China, 2021. (In Chinese), articleTitle=null, refAbstract=null), Reference(id=1241826555861734384, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=null, issue=32, pageStart=13, pageEnd=15, 19, url=null, language=null, rfNumber=［14］, rfOrder=15, authorNames=汪忆梦, 张凤娟, 于明明, journalName=食品安全导刊, refType=null, unstructuredReference=汪忆梦，张凤娟，于明明，等．2022年潍坊市肉与肉制品中单增李斯特菌污染状况分析［J］．食品安全导刊, 2023, (32): 13-15, 19., articleTitle=2022年潍坊市肉与肉制品中单增李斯特菌污染状况分析, refAbstract=null), Reference(id=1241826555941426161, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=null, issue=32, pageStart=13, pageEnd=15, 19, url=null, language=null, rfNumber=［14］, rfOrder=16, authorNames=Wang YM, Zhang FJ, Yu MM, journalName=China Food Safety Magazine, refType=null, unstructuredReference=Wang YM, Zhang FJ, Yu MM, et al. Analysis on contamination of Listeria monocytogenes in meat and meat products sold in Weifang in 2022［J］. China Food Safety Magazine, 2023, (32): 13-15, 19. (In Chinese), articleTitle=Analysis on contamination of Listeria monocytogenes in meat and meat products sold in Weifang in 2022, refAbstract=null), Reference(id=1241826556033700850, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=39, issue=10, pageStart=941, pageEnd=950, url=null, language=null, rfNumber=［15］, rfOrder=17, authorNames=林慧琳, 叶玲清, 叶海梅, journalName=中国人兽共患病学报, refType=null, unstructuredReference=林慧琳，叶玲清，叶海梅，等．2011—2021年福建省即食食品中单增李斯特菌的监测及菌株特征分析［J］．中国人兽共患病学报, 2023, 39(10): 941-950., articleTitle=2011—2021年福建省即食食品中单增李斯特菌的监测及菌株特征分析, refAbstract=null), Reference(id=1241826556113392627, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=39, issue=10, pageStart=941, pageEnd=950, url=null, language=null, rfNumber=［15］, rfOrder=18, authorNames=Lin HL, Ye LQ, Ye HM, journalName=Chinese Journal of Zoonoses, refType=null, unstructuredReference=Lin HL, Ye LQ, Ye HM, et al. Monitoring of listeria monocytogenes in ready-to-eat foods and characterization of isolates in Fujian province from 2011 to 2021［J］. Chinese Journal of Zoonoses, 2023, 39(10): 941-950. (In Chinese), articleTitle=Monitoring of listeria monocytogenes in ready-to-eat foods and characterization of isolates in Fujian province from 2011 to 2021, refAbstract=null), Reference(id=1241826556193084404, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2020, volume=312, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［16］, rfOrder=19, authorNames=Liu YT, Sun WX, Sun TM, journalName=International Journal of Food Microbiology, refType=null, unstructuredReference=Liu YT, Sun WX, Sun TM, et al. The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach［J］. International Journal of Food Microbiology, 2020, 312: 108358., articleTitle=The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach, refAbstract=null), Reference(id=1241826556331496437, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2021, volume=7, issue=2, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［17］, rfOrder=20, authorNames=Bouymajane A, Rhazi filali F, Oulghazi S, journalName=Heliyon, refType=null, unstructuredReference=Bouymajane A, Rhazi filali F, Oulghazi S, et al. Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods［J］. Heliyon, 2021, 7(2): e06169., articleTitle=Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods, refAbstract=null), Reference(id=1241826556398605302, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2019, volume=10, issue=null, pageStart=1710, pageEnd=null, url=null, language=null, rfNumber=［18］, rfOrder=21, authorNames=Zhang YY, Dong SL, Chen HH, journalName=Frontiers in Microbiology, refType=null, unstructuredReference=Zhang YY, Dong SL, Chen HH, et al. Prevalence, genotypic characteristics and antibiotic resistance of listeria monocytogenes from retail foods in bulk in Zhejiang province, China［J］. Frontiers in Microbiology, 2019, 10: 1710., articleTitle=Prevalence, genotypic characteristics and antibiotic resistance of listeria monocytogenes from retail foods in bulk in Zhejiang province, China, refAbstract=null), Reference(id=1241826556478297079, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2024, volume=36, issue=4, pageStart=426, pageEnd=432, url=null, language=null, rfNumber=［19］, rfOrder=22, authorNames=蔡炯, 陈潇, 朱虹霖, journalName=中国食品卫生杂志, refType=null, unstructuredReference=蔡炯，陈潇，朱虹霖，等．成都市冷藏即食预包装熟肉制品中单核细胞增生李斯特菌定量检测及污染风险研究［J］．中国食品卫生杂志, 2024, 36(4): 426-432., articleTitle=成都市冷藏即食预包装熟肉制品中单核细胞增生李斯特菌定量检测及污染风险研究, refAbstract=null), Reference(id=1241826556549600248, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2024, volume=36, issue=4, pageStart=426, pageEnd=432, url=null, language=null, rfNumber=［19］, rfOrder=23, authorNames=Cai J, Chen X, Zhu HL, journalName=Chinese Journal of Food Hygiene, refType=null, unstructuredReference=Cai J, Chen X, Zhu HL, et al. Research on quantitative and contamination level of Listeria monocytogenes from pre-packaged refrigerated ready-to-eat meat in Chengdu city［J］. Chinese Journal of Food Hygiene, 2024, 36(4): 426-432. (In Chinese), articleTitle=Research on quantitative and contamination level of Listeria monocytogenes from pre-packaged refrigerated ready-to-eat meat in Chengdu city, refAbstract=null), Reference(id=1241826556637680633, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=13, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［20］, rfOrder=24, authorNames=Zhu L, Ji X, Wu Y, Huang X, journalName=Frontiers in Cellular and Infection Microbiology, refType=null, unstructuredReference=Zhu L, Ji X, Wu Y, et al, Huang X. Molecular characterization of Listeria monocytogenes strains isolated from imported food in China from 14 countries/regions, 2003-2018［J］. Frontiers in Cellular and Infection Microbiology, 2023, 13: 1287564., articleTitle=Molecular characterization of Listeria monocytogenes strains isolated from imported food in China from 14 countries/regions, 2003-2018, refAbstract=null), Reference(id=1241826556717372410, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2019, volume=8, issue=1, pageStart=1195, pageEnd=1204, url=null, language=null, rfNumber=［21］, rfOrder=25, authorNames=Gan L, Cao XL, Wang Y, journalName=Emerging Microbes & Infections, refType=null, unstructuredReference=Gan L, Cao XL, Wang Y, et al. Carriage and potential long distance transmission of Listeria monocytogenes by migratory black-headed gulls in Dianchi Lake, Kunming［J］. Emerging Microbes & Infections, 2019, 8(1): 1195-1204., articleTitle=Carriage and potential long distance transmission of Listeria monocytogenes by migratory black-headed gulls in Dianchi Lake, Kunming, refAbstract=null), Reference(id=1241826556792869883, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, doi=null, pmid=null, pmcid=null, year=2023, volume=37, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［22］, rfOrder=26, authorNames=Moura A, Leclercq A, Vales G, journalName=The Lancet Regional Health. Europe, refType=null, unstructuredReference=Moura A, Leclercq A, Vales G, et al. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France［J］. The Lancet Regional Health. Europe, 2023, 37: 100800., articleTitle=Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France, refAbstract=null)], funds=[Fund(id=1241826552703423444, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, awardId=2018GXNSFAA050039, language=CN, fundingSource=广西自然科学基金项目(2018GXNSFAA050039), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1241826546697179954, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, xref=null, ext=[AuthorCompanyExt(id=1241826546713957176, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China), AuthorCompanyExt(id=1241826546726540088, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, companyId=1241826546697179954, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=广西壮族自治区疾病预防控制中心，广西　南宁　530028)])], figs=[ArticleFig(id=1241826551340274614, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Fig.1, caption=The minimum spanning tree of the 23 ST’s from Listeria monocytogenes in Guangxi, figureFileSmall=UiI2nWtc2hRJrOQiWb4faw==, figureFileBig=VTf39rrZuzckkS1r1U58Ng==, tableContent=null), ArticleFig(id=1241826551428355000, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=图1, caption=广西即食食品单增李斯特菌23个STs最小生成树, figureFileSmall=UiI2nWtc2hRJrOQiWb4faw==, figureFileBig=VTf39rrZuzckkS1r1U58Ng==, tableContent=null), ArticleFig(id=1241826551533212602, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Fig.2, caption=The minimum spanning tree of the STs and CC from 158 Listeria monocytogenes in Guangxi, figureFileSmall=Qzh8uK66VrFTurJzHJwIFA==, figureFileBig=qysCkjUUYdbo3348YX6saA==, tableContent=null), ArticleFig(id=1241826551675818942, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=图2, caption=广西158株单增李斯特菌CC型和ST型的最小生成树, figureFileSmall=Qzh8uK66VrFTurJzHJwIFA==, figureFileBig=qysCkjUUYdbo3348YX6saA==, tableContent=null), ArticleFig(id=1241826551772287935, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Table 1, caption=

Distribution of serotypes of L.monocytogenes isolates from RTE foods in Guangxi 2011-2020

, figureFileSmall=null, figureFileBig=null, tableContent=
血清型熟肉制品焙烤及油炸类食品中式凉拌菜寿司冷冻饮品水产品混合食品蔬菜及沙拉总计
1/2a,3a2512811721167
1/2b,3b,727581252151
4b,4d,4e9630202022
1/2c,3c9430020018
总计7027221211952158
), ArticleFig(id=1241826551860368321, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=表1, caption=

2011—2020年广西即食食品中单增李斯特菌血清型分布情况

, figureFileSmall=null, figureFileBig=null, tableContent=
血清型熟肉制品焙烤及油炸类食品中式凉拌菜寿司冷冻饮品水产品混合食品蔬菜及沙拉总计
1/2a,3a2512811721167
1/2b,3b,727581252151
4b,4d,4e9630202022
1/2c,3c9430020018
总计7027221211952158
), ArticleFig(id=1241826551998780357, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Table 2, caption=

Distribution of ST of L.monocytogenes isolates from RTE foods (Top 10)

, figureFileSmall=null, figureFileBig=null, tableContent=
STsCC谱系血清型菌株数/(%)
ST8CC8II1/2a,3a37（23.42）
ST87CC87I1/2b,3b,723（14.56）
ST145CC2I4b,4d,4e15（9.49）
ST9CC9II1/2c,3c12（7.59）
ST101CC101II1/2a,3a10（6.33）
ST3CC3I1/2b,3b,79（5.70）
ST121CC121II1/2a,3a8（5.06）
ST122CC122II1/2c,3c6（3.80）
ST31CC31II1/2a,3a4（2.53）
ST5CC5I1/2b,3b,74（2.53）
ST330CC288I1/2b,3b,74（2.53）
ST1CC1I4b,4d,4e4（2.53）
), ArticleFig(id=1241826552095249350, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=表2, caption=

即食食品中单增李斯特菌ST型分布情况（前10）

, figureFileSmall=null, figureFileBig=null, tableContent=
STsCC谱系血清型菌株数/(%)
ST8CC8II1/2a,3a37（23.42）
ST87CC87I1/2b,3b,723（14.56）
ST145CC2I4b,4d,4e15（9.49）
ST9CC9II1/2c,3c12（7.59）
ST101CC101II1/2a,3a10（6.33）
ST3CC3I1/2b,3b,79（5.70）
ST121CC121II1/2a,3a8（5.06）
ST122CC122II1/2c,3c6（3.80）
ST31CC31II1/2a,3a4（2.53）
ST5CC5I1/2b,3b,74（2.53）
ST330CC288I1/2b,3b,74（2.53）
ST1CC1I4b,4d,4e4（2.53）
), ArticleFig(id=1241826552212689866, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Table 3, caption=

MIC Distribution of 158 L.monocytogenes lsolates

, figureFileSmall=null, figureFileBig=null, tableContent=
抗生素最小抑菌浓度MIC/(μg/mL)菌株数量μg/mL耐药率(%)
321684210.50.250.1250.060.030.0150.007MIC50MIC90
AMP6539720.2500.5000.00
PEN1651801010.5001.0000.00
TET30982641.0002.0000.00
MEM4118360.1250.1250.00
SXT4122096260.0150.0300.00
ERY32112940.1250.2500.63
VAN2013431.0002.0000.63
CIP1113111.0001.0002.53
), ArticleFig(id=1241826552309158859, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=表3, caption=

158株单增李斯特菌药敏结果

, figureFileSmall=null, figureFileBig=null, tableContent=
抗生素最小抑菌浓度MIC/(μg/mL)菌株数量μg/mL耐药率(%)
321684210.50.250.1250.060.030.0150.007MIC50MIC90
AMP6539720.2500.5000.00
PEN1651801010.5001.0000.00
TET30982641.0002.0000.00
MEM4118360.1250.1250.00
SXT4122096260.0150.0300.00
ERY32112940.1250.2500.63
VAN2013431.0002.0000.63
CIP1113111.0001.0002.53
), ArticleFig(id=1241826552393044941, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=EN, label=Table 4, caption=

Acquired resistance among 158 isolates of Guangxi RTE foodL. movocytogenes

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株血清型年度来源最小抑菌浓度MIC/(μg/mL)获得性耐药基因
ERYVANCIP
DZ20170801/2b,3b,72017中式凉拌菜8tet(M)、msr(D)、mef(A)
DZ20160281/2a,3a2016冷冻饮品8/
DZ20160144b,4d,4e2016混合食品（盒饭）4/
DZ20160261/2b,3b,72016水产品4/
DZ20180301/2a,3a2018寿司8/
DZ20180311/2a,3a2018寿司8/
), ArticleFig(id=1241826552485319631, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241675631495278635, language=CN, label=表4, caption=

158株广西即食食品中单增李斯特菌获得耐药性菌株情况

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株血清型年度来源最小抑菌浓度MIC/(μg/mL)获得性耐药基因
ERYVANCIP
DZ20170801/2b,3b,72017中式凉拌菜8tet(M)、msr(D)、mef(A)
DZ20160281/2a,3a2016冷冻饮品8/
DZ20160144b,4d,4e2016混合食品（盒饭）4/
DZ20160261/2b,3b,72016水产品4/
DZ20180301/2a,3a2018寿司8/
DZ20180311/2a,3a2018寿司8/
)], attaches=null, journal=Journal(id=1227664546253402114, delFlag=0, nameCn=现代预防医学, nameEn=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, issn=1003-8507, eissn=null, cn=51-1365/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=IeiuPXEZi6AA+k0VfvoiOQ==, journalPrice=null, startedYear=null, abbrevIsoEn=Modern Preventive Medicine, journalRemark=null, publicationField=null, createdTime=1770627636734, updatedTime=1770628902248, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=M, firstLetterEn=M, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=IeiuPXEZi6AA+k0VfvoiOQ==, picEn=/9iTl8/ndms4tBz1fL28Pg==, jcr=null, cjcr=null, exts=[JournalExt(id=1227669854342280188, language=CN, name=现代预防医学, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902278, updatedTime=1770628902278, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1227669854396806141, language=EN, name=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902291, updatedTime=1770628902291, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1227665162245664772, websiteList=[Website(id=1227687234141352800, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/CN, language=CN, createTime=1770633045945, createBy=18614031015, updateTime=1770633090526, updateBy=18614031015, name=现代预防医学-中文, tplId=1146099689490845704, title=现代预防医学, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687735088051072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=articleTextType, value=kx, createTime=1770633165380, updateTime=1770633165380, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735071273853, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=banner, value=null, createTime=1770633165376, updateTime=1770633165376, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735113216899, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=grayFlag, value=0, createTime=1770633165386, updateTime=1770633165386, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735062885244, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633165374, updateTime=1770633165374, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735125799813, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=minRunFlag, value=0, createTime=1770633165389, updateTime=1770633165389, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735083856767, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic, createTime=1770633165379, updateTime=1770633165379, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735121605508, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=silenceFlag, value=0, createTime=1770633165388, updateTime=1770633165388, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735079662462, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1770633165378, updateTime=1770633165378, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735096439681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeColor, value=null, createTime=1770633165382, updateTime=1770633165382, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735104828290, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeStyle, value=null, createTime=1770633165384, updateTime=1770633165384, creator=18614031015, updator=18614031015)]), Website(id=1227687234338485094, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/EN, language=EN, createTime=1770633045992, createBy=18614031015, updateTime=1770633115374, updateBy=18614031015, name=现代预防医学-英文, tplId=1146101810881728533, title=Modern Preventive Medicine, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687709129507332, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=articleTextType, value=kx, createTime=1770633159191, updateTime=1770633159191, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709108535809, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=banner, value=null, createTime=1770633159186, updateTime=1770633159186, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709167256071, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=grayFlag, value=0, createTime=1770633159200, updateTime=1770633159200, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709095952896, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633159183, updateTime=1770633159183, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709179838985, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=minRunFlag, value=0, createTime=1770633159203, updateTime=1770633159203, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709121118723, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic, createTime=1770633159189, updateTime=1770633159189, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709171450376, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=silenceFlag, value=0, createTime=1770633159201, updateTime=1770633159201, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709112730114, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1770633159187, updateTime=1770633159187, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709133701637, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeColor, value=null, createTime=1770633159192, updateTime=1770633159192, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709154673158, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeStyle, value=null, createTime=1770633159197, updateTime=1770633159197, creator=18614031015, updator=18614031015)])], journalTitle=现代预防医学, weixinUrl=null, journalUrl=http://xdyfyxzz.paperopen.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Modern Preventive Medicine, journalPhotoCn=IeiuPXEZi6AA+k0VfvoiOQ==, journalPhotoEn=/9iTl8/ndms4tBz1fL28Pg==, journalFirstLetter=M, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202404454, detailUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/10.20043/j.cnki.MPM.202404454, pdfUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/PDF/10.20043/j.cnki.MPM.202404454, pdfUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/PDF/10.20043/j.cnki.MPM.202404454, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
2011—2020年广西即食食品中单增李斯特菌耐药性、毒力和分子特征研究
收藏切换
PDF下载
曾献莹 , 吕素玲 , 谭冬梅 , 瞿聪 , 李秀桂
现代预防医学 | 营养与食品卫生 2024,51(22): 4080-4085
收起
收藏切换
现代预防医学 | 营养与食品卫生 2024, 51(22): 4080-4085
2011—2020年广西即食食品中单增李斯特菌耐药性、毒力和分子特征研究
全屏
曾献莹, 吕素玲 , 谭冬梅, 瞿聪, 李秀桂
作者信息
  • 广西壮族自治区疾病预防控制中心，广西　南宁　530028

    • 曾献莹（1989—），女，硕士，副主任技师，研究方向：食源性致病菌相关研究

通讯作者:

吕素玲，E-mail:
Antimicrobial resistance, virulence profile, and molecular characterization of Listeriamonocytogenes isolated from ready-to-eat food, Guangxi, 2011-2020
Xian-ying ZENG, Su-ling LV , Dong-mei TAN, Cong QU, Xiu-gui LI
Affiliations
  • Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi 530028, China
出版时间: 2024-11-25 doi: 10.20043/j.cnki.MPM.202404454
文章导航
收藏切换
摘要
收起
目的

分析广西即食食品中单核细胞增生李斯特菌（以下简称单增李斯特菌）的耐药性、分子特征和毒力特征，更好地了解即食食品中单增李斯特菌的遗传特点、耐药情况及其潜在的健康风险。

方法

采用肉汤稀释法对158株即食食品中单增李斯特进行药敏测定，同时对其进行全基因组测序（WGS），分析其序列ST型、血清群、血清型、CC型、谱系、耐药基因及毒力基因。

结果

2011—2020年广西即食食品中单增李斯特菌以1/2a,3a血清型为主，优势ST型为ST8/CC8和ST87/CC87。研究菌株均对AMP、PEN、TET、MEM、SXT敏感，4株分离株耐受CIP，2株单增李斯特菌分别耐受ERY和VAN。仅对红霉素耐药的1株分离株携带tet(M)、msr(D)、mef(A)耐药基因。actA、inlF、inlK的携带率分别为79.74%、48.73%、55.69%，其余毒力基因携带率为100%。46.20%（73/158）的菌株携带12个毒力基因，24株高毒力单增李斯特菌产生潜在高致病菌株。

结论

广西即食食品中单增李斯特菌存在的获得性耐药基因和高毒力基因对公众健康构成严重风险，应该对即食食品加工和服务环境采取有效的食品安全措施。

单增李斯特菌  /  全基因组测序  /  抗生素耐药性  /  毒力基因
Objective

To comprehensively analyze the occurrence, virulence characteristics, and molecular typing of Listeria monocytogenes (L.monocytogenes) isolated from ready-to-eat (RTE) food in Guangxi. By investigating the genetic attributes, antibiotic resistance and potential health risks of L.monocytogenes, this research contributes to our understanding of this pathogen.

Methods

We analyzed 158 isolates of L. monocytogenes from RTE foods collected in Guangxi between 2011 and 2020. Whole genome sequencing (WGS) was performed on all isolates and antimicrobial resistance were determined by broth microdilution.The study included the determination of sequence types (STs), serotype, serogroup, lineage, clonal complexes (CC), acquired resistance genes and the presence of L. monocytogenes pathogenicity islands (LIPIs).

Results

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi. The predominant serotypes were 1/2a, 3a.ST8/CC8 and ST87/CC87 were the dominant sequence types (STs) in RTE foods.All isolates were found to be susceptible to ampicillin(AMP), penicillin(PEN), tetracycline(TET), meropenem(MEM), and trimethoprim-sulfamethoxazole(SXT). Four isolates were resistant to ciprofloxacin. Two isolates wereresistant to erythromycin and vancomycin respectively. Acquired antimicrobial phenotypes were tet (M), msr (D) and mef (A) of an isolate resistant to erythromycin. Most of the isolates carried actA (79.74%), inlF (48.73%), inlK (55.69%)and all isolates carried other virulence genes.46.20%(73/158)of the isolatesharboured 12 virulence genes.It can be a potential public health risk due to its high harboring rate of 17 or 18 virulence genes.

Conclusion

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi.Acquired resistance genes and hypervirulence genes existed in this bacterium from RTE foods origins, and can be a major risk for consumers and public health.It was suggested that effective food safety measures should be implemented in RTE foods processing and service environments.

Listeria monocytogenes  /  Whole genome sequencing (WGS)  /  Antibiotic resistance  /  Virulence genes
曾献莹, 吕素玲, 谭冬梅, 瞿聪, 李秀桂. 2011—2020年广西即食食品中单增李斯特菌耐药性、毒力和分子特征研究. 现代预防医学, 2024 , 51 (22) : 4080 -4085 . DOI: 10.20043/j.cnki.MPM.202404454
Xian-ying ZENG, Su-ling LV, Dong-mei TAN, Cong QU, Xiu-gui LI. Antimicrobial resistance, virulence profile, and molecular characterization of Listeriamonocytogenes isolated from ready-to-eat food, Guangxi, 2011-2020[J]. Modern Preventive Medicine, 2024 , 51 (22) : 4080 -4085 . DOI: 10.20043/j.cnki.MPM.202404454
正文
收起
由于具有高死亡率，侵袭性李斯特氏菌病是最严重的食源性疾病之一[1]。单核细胞增生李斯特氏菌（简称单增李斯特菌）是导致侵袭性李斯特氏菌病的重要原因。侵袭性李斯特菌病的高危人群是孕妇，婴儿，老年人或免疫功能低下人群，会出现败血症、脑膜炎、早产、流产和死胎等症状[2]。全球范围内李斯特菌病死亡率高达20% 至40%[3]。目前，至少已鉴定出13种单增李斯特菌血清型（1/2a,1/2b,1/2c,3a,3b,3c,4a,4b,4c,4e,7)和四个遗传多样性谱系（Ⅰ-Ⅳ），谱系Ⅰ和Ⅱ是引起人类疾病的主要谱系[4-6]。血清型1/2a、1/2b 、1/2c和4b是中国食品来源单增李斯特菌优势血清型[7]。
随着抗生素的广泛使用，食品中的单增李斯特菌也出现多重耐药的情况[8]。另外，通过基因水平转移或突变获得耐药基因使单增李斯特菌获得耐药性[9]。这些耐药菌株可能通过食物链的形式传播给人类，因此监测单增李斯特菌的耐药性及耐药基因是十分必要的。
即食食品(Ready-to-eat, RTE)被认为是感染李斯特菌病的主要来源。即食食品是不经处理(清洗或烹煮)进行食用且长时间存放在冰箱或冷藏温度条件下的食品。由于单增李斯特菌能够形成生物膜，在冷藏温度(2℃-4℃)下生长并能耐受高浓度的NaCl[7]，因此单增李斯特菌易于在即食食品中生长。而即食食品种类繁多，目前没有相关报道广西即食食品中单增李斯特菌的污染情况。
全基因组测序（whole genome sequencing, WGS）是深入分析细菌分离株的有力工具。本研究对广西即食食品中单增李斯特菌进行全基因组测序，分析其分子特征、耐药基因和毒力基因，揭示其耐药特点和分子遗传多样性，了解广西即食食品中单增李斯特菌的污染情况，确定潜在的高风险即食食品种类，为其风险防控提供参考数据。
1　材料与方法
收起
1.1　材料
1.1.1　菌株来源
158株即食食品来源单增李斯特菌来自2011—2020年广西壮族自治区各县市疾控中心分离上报，经广西壮族自治区疾病预防控制中心微生物检验所鉴定复核。根据样品类别特性，158株单增李斯特菌分属8种即食食品种类：熟肉制品来源70株、中式凉拌菜来源22株、焙烤及油炸类食品来源27株、寿司来源12株、冷冻饮品来源11株、水产品来源9株、混合食品来源5株和蔬菜沙拉来源2株。
药敏试验质控菌株为金黄色葡萄球菌ATCC29213 (Staphylococcus aureus)和肺炎链球菌ATCC49619(Streptococcus pneumonia)。
1.1.2　主要试剂和仪器
脑心浸液肉汤(BHI)、脑心浸液琼脂(BHA)、DNA 提取试剂盒(Qiagen , 德国)、李斯特菌定制药敏检测板(上海星佰生物技术有限公司)，均在有效期内使用。Qubit Fluorometer 2.0(Invitrogen, 美国)、Hiseq X Ten(Illumina, 美国)、小型高速离心机(Eppendorf , 德国) 、恒温培养箱(3M,美国)。
1.2　实验方法
1.2.1　药敏实验
按照药敏试剂盒使用说明，采用微量肉汤法定量测定抗生素MIC，包含七类8种抗生素：青霉素类：氨苄西林(Ampicillin，AMP)、青霉素（Penicillin，PEN）；大环内酯类：红霉素（Erythromycin，ERY）；四环素类：四环素(Tetracycline，TET)； 碳青霉烯类：美罗培南（Meropenem，MEM）；氟喹诺酮类：环丙沙星(Ciprofloxazole，CIP)；复方磺胺类：复方新诺明（Trimethoprim/Sulfamethoxazole，SXT）；氨基糖苷类：万古霉素（Vancomycin，VAN）。按照CLSI和EUCAST标准判读结果并确定耐药表型[10-11]。
1.2.2　全基因组测序
选取BHA上的单菌落接种BHI过夜培养，使用全基因组DNA提取试剂盒对待测菌株进行DNA提取，质控检测合格的DNA经超声波破碎仪打断成长度约为350 bp的片段，经末端修复，加A尾碱基，将测序接头连接到DNA片段，纯化、PCR扩增后完成整个文库的制备。文库构建完成后，使用Illumina公司的Hiseq X Ten测序平台应用 PE150上机试剂盒进行测序，测序数据质量要求为：基因组覆盖度≥95%，基因区覆盖度≥98%，整体覆盖深度≥100×，碱基数据质量值Q30≥85%。将低质量reads过滤后，将raw data导入国家食源性疾病分子溯源网络（TraNet）进行组装和分析。是基于BioNumerics 7.6（BN）软件建立的，分析后的测序数据将下载至该软件数据库里。
1.2.3　谱系、血清和MLST分析
根据WGS的结果进行试验菌株的序列分型(Sequence typing, ST) 、血清和谱系分析。组装和拼接好的denovo 数据从BioNumerics 7.6软件数据库里导出，利用 TraNet 数据交付平台中的MLST 分型模块、李斯特菌模块进行分析，即可获得待测菌株的血清群和ST型。多位点序列分析中7个等位基因中仅 1个位点不同定义为同一克隆复合体(Clonal complex, CC)。依据巴斯德网站上公布的ST-CC-Lineage 表确定谱系。
1.2.4　毒力基因和耐药基因的分析
使用抗性基因数据库CARD和BN 7.6软件查找待测菌株所携带的耐药基因。毒力基因分布主要通过毒力基因数据库VFDB和BIGSdb-Lm 数据库获得，选择毒力基因包括LIPI-1（prfA、hly、plcB、plcA、mpl、actA）、LIPI-2（inlA、inlB、inlF、inlK、inlC、inlJ）和 LIPI-3（llsA、llsD、llsP、llsH、llsY、llsB、llsX、llsG），比对设定核苷酸最小一致性为 80%。
1.2.5　统计学分析
熟肉制品与非熟肉制品血清型数据差异运用SPSS Statistics 25.0统计软件进行分析，使用卡方检验，检验水准α=0.05。
2　结果
收起
2.1　2011—2020年广西即食食品中单增李斯特菌血清型和ST分型分布特点
所有分离株分为4个不同的血清型1/2a,3a、1/2b,3b,7、1/2c,3c、4b,4d,4e (表1)。主要血清型为1/2a,3a (67,42.41%) ，其次为1/2b,3b,7 (51,32.28%) ，4b,4d,4e (22,13.92%)和1/2c,3c (18,11.39%) 。熟肉制品中检出的优势血清型为1/2b,3b,7，占比38.57%(27/70)，熟肉制品与非熟肉制品血清型分布无统计学意义（χ2=3.208，P=0.361>0.05）。
158株单增李斯特菌中73株（46.20%）分布于谱系I和85株（53.79%）分布于谱系II。4个血清群分布在两个谱系之间， II b（32.28%）和IV b（13.92%）属于谱系I， II a（42.41%）和II c（11.39%）属于谱系II（图1）。
158株单增李斯特菌分为23个不同的ST型，进一步分析为18个CC型和1个单体（表2，图2）。ST8/CC8占比23.42%，是本研究的优势ST型。其他ST型分别是ST87/CC87（14.56%）、ST145/CC2（9.49%）、ST9/CC9（7.59%）和ST101/CC101（6.33%）。
2.2　2011—2020年广西即食食品中单增李斯特菌抗生素耐药特点
158株单增李斯特菌8种不同类别抗生素的最小抑菌浓度MIC分布值（其中50%（MIC50）或90%（MIC90）的生长受到抑制）见表3。158株单增李斯特菌对AMP、PEN、TET、MEM、SXT敏感，2.53%分离株对CIP有耐药性，0.63%分离株分别对ERY、VAN有耐药性。如表4所示，6株分离株（158株测试菌株的3.8%）具有耐药性：1株对ERY、1株对VAN、4株对CIP。
对158株单增李斯特菌进行抗生素抗性基因的预测，根据基因匹配识别度90%以上的原则，所有单增李斯特菌均携带FosX、lin、mprF基因，42株（26.58%）携带norB基因，所有菌株均未携带blaA基因。对ERY耐药的1株单增李斯特菌携带在tet(M)、msr(D) 、mef(A)等耐药基因。5株对VAN和CIP耐药的分离株未携带其他耐药基因。
2.3　2011—2020年广西即食食品中单增李斯特菌毒力基因分布特点
158株单增李斯特菌对LIPI 1中的 prfA、hly、plcB、plcA、mpl基因的携带率是100%，actA基因携带率79.74%（126/158）。LIPI 2中毒力基因inlA、inlB、inlC和inlJ的携带率均为100%，毒力基因inlF、inlK的携带率分别为48.73%、55.69%。24株（15.18%）分离株携带LIPI 3的8个毒力基因。携带9种、10种、11种、12种、17种、18种毒力基因的分离株比例为2.53%（4/158）、33.54%（53/158）、2.53%（4/158）、46.20%（73/158）、8.23% （13/158）、6.96%（11/158）。46.20%的菌株携带12个毒力基因，血清型主要分布在1/2a,3a（55）和1/2c, 3c（18），主要来源于熟肉制品、焙烤及油炸类食品和中式凉拌菜。高毒力菌株为携带17或18个毒力基因的菌株，以缺失actA或（和）inlF、inlK为主要特征，血清型以1/2b,3b,7为主，分布在熟肉制品等六个样品类别。
3　讨论
收起
李斯特菌病是一种主要通过即食食品传播的高死亡率的食源性疾病。现代快节奏生活方式的改变使人们用于准备食物的时间减少，对即食食品和外卖食品的需求增加，因此通过即食食品感染单增李斯特菌的风险也增加。本研究即食食品中单增李斯特菌主要来源于熟肉制品，其次是焙烤及油炸类食品和中式凉拌菜，与国家监测的结果是基本一致的[12]。国家标准GB31607-2021《散装即食食品中致病菌限量》对部分或未经热处理的散装即食食品中规定不得检出单核细胞增生李斯特菌[13]。这表明熟肉制品来源的单增李斯特菌是污染即食食品的高风险菌株。焙烤及油炸类食品经高温制作后依然存在感染单增李斯特菌的风险，有可能是制作过程中存在生熟交叉污染的可能。单增李斯特菌在低温条件下能大量繁殖，中式凉拌菜常于低温储存易于污染，因此应该对冷藏地点进行适当清洗并定期消毒，建议于低温存放的食品应加热后再食用。
对即食食品中单增李斯特菌进行血清分型，有助于初步识别其与食物类别潜在健康风险。本研究中8类即食食品分属于谱系I（46.20%）和谱系II（53.79%），优势血清型为1/2a,3a，这与国内其他省市研究的结果一致[14-16]。21世纪初，谱系II血清型1/2a的单增李斯特菌已经取代血清型4b，成为引起人李斯特菌病的主要血清型，这可能是由于这种血清型在日益流行的即食食品中频繁检出有关[17]。血清型1/2b,3b,7主要分布在病人来源菌株中，且基因多态性最低，重组水平最低。而本研究中熟肉制品中单增李斯特菌血清型1/2b,3b,7超过1/2a,3a成为优势血清型，下一步我们应该进一步探究熟肉制品中血清型变化的原因。
WGS分析显示广西即食食品中单增李斯特菌分为23个ST型，ST8/CC8（23.42%）、ST87/CC87（14.56%）为优势ST型，与其他研究报道的我国优势型别（ST8、ST9、ST87、ST101）相近[8,18-20]。值得注意的是，本研究中ST145/CC2占比9.49%，谱系I，血清型为4b,4d,4e，该型别在国内外鲜有报道。国内有关研究在昆明滇池湖中的红嘴鸥中检出ST145，认为该型别可能沿着鸟类迁徙路线传播[21]。本研究中该型别分布于焙烤及油炸类食品，超过ST9/CC9成为广西即食食品中单增李斯特菌主要的ST型别，这表明以该型别为主的焙烤及油炸类食品对消费者构成潜在的健康风险，提醒我们持续监测和关注ST145型别的单增李斯特菌暴发情况。
单增李斯特菌对大多数抗生素敏感，治疗李斯特菌病一线抗生素是PEN和AMP。SXT，ERY，VAN和氟喹诺酮类药物被用作治疗李斯特菌病的二线药物，特别用于青霉素过敏的患者。本研究中我们观察到所有菌株对AMP、PEN、TET、MEM和SXT敏感，3.8%的分离株对ERY、VAN、CIP有不同程度的耐药。本研究待测菌株均携带FosX、lin、mprF基因，这与文献报道的一致[21]。26.58%的分离株携带norB（编码喹诺酮类）基因但未表现出环丙沙星的耐药性，1株对万古霉素、4株对环丙沙星耐药的测序分离株未携带其他耐药基因，这种表型和基因型的不一致性可能是因为喹诺酮类药物存在多种耐药机制以及耐药基因存在复杂的调控机制[22]。对红霉素耐药的1株分离株携带tet(M)、msr(D)、mef(A)等耐药基因，但未显示出四环素耐药性。该分离株来源于中式凉拌菜，血清型为1/2b,3b,7，由于即食食品即食入口，一旦感染单增李斯特菌会产生严重的疾病症状，这要求我们应定期监测单增李斯特菌的耐药情况，以便及早发现即食食品中抗生素耐药情况的变化。
单增李斯特菌毒力基因的存在可能与其致病程度有关联。本研究中有32株分离株缺失actA基因，其中的13株携带LIPI-3的毒力基因，血清型以1/2b, 3b,7为主，以熟肉制品来源为主，均为谱系I，这与LIPI-3只存在于谱系I的研究结论是一致的[7]。有研究表明，血清型1/2b的菌株不需要完整功能的ActA 蛋白也可以引起小鼠死亡，提示可能存在其他机制使毒力因子与细胞互相作用。因此，广西即食食品中缺失actA基因的单增李斯特菌依然存在使人致病的风险。所有的分离株至少携带9个以上的毒力基因，46.20%的分离株携带完整的LIPI-1和LIPI-2毒力基因，这与单增李斯特菌中毒力基因相对保守有关，提示我们广西即食食品中的单增李斯特菌具有一定程度的致病性，应该加强食品安全风险监测。高毒力菌株（含17/18个毒力基因）主要是缺失actA或（和）inlF、inlK基因，分布于熟肉制品、焙烤及油炸食品、水产品、中式凉拌菜、蔬菜沙拉及混合食品，高毒力的单增李斯特菌所污染的即食食品可能导致人类面临严重的食源性疾病甚至死亡。
本研究旨在通过抗生素耐药测定和全基因组测序分析广西即食食品中单增李斯特菌耐药趋势、分子特征及毒力特性，以识别其潜在的致病性和耐药性。结果显示，广西即食食品中熟肉制品污染率较高，以血清型1/2b,3b,7，ST87/CC87为主，这提示熟肉制品是单增李斯特菌污染高风险食物，建议食用前充分加热，高危人群应尽量避免食用。所检测的分离株均携带毒力基因，存在潜在的致病风险，分离株耐药水平不高，少数菌株存在获得性耐药基因。因此，我们应加强即食食品特别是熟肉制品中单增李斯特菌的监测，揭示其致病机制以助于有效预防，降低其对消费者和公共卫生健康构成重大风险。
基金
收起
  • 广西自然科学基金项目(2018GXNSFAA050039)
文献
收起
参考文献 引证文献
排序方式：
［1］
European Food Safety Authority, European Centre for Disease Prevention and Control. The European Union one health 2022 zoonoses report［J］. EFSA Journal. European Food Safety Authority, 2023, 21(12): e8442.
［2］
World Health Organization. Listeriosis［EB/OL］. ［2024-09-19］. https://www.who.int/news-room/fact-sheets/detail/listeriosis.
［3］
Niu YL, Li WW, Xu BY, et al. Risk factors associated with food consumption and food-handling habits for sporadic listeriosis: a case-control study in China from 2013 to 2022［J］. Emerging Microbes & Infections, 2024, 13(1): 2307520.
［4］
Tricoli MR, Massaro C, Arrigo I, et al. Characterization of listeria monocytogenes strains isolated in palermo (Sicily and Italy) during the years 2018-2020 from severe cases of listeriosis［J］. Antibiotics, 2024, 13(1): 57.
［5］
Heidarlo MN, Lotfollahi L, Yousefi S, et al. Analysis of virulence genes and molecular typing of Listeriamonocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran［J］. Tropical Animal Health and Production, 2021, 53(1): 127.
［6］
World Health Organization. Listeria monocytogenes in ready-to-eat (RTE) foods : Attribution, characterization and monitoring: Meeting report［EB/OL］. ［2024-09-19］. https://www.who.int/publications/i/item/9789240034969.
［7］
Li WW, Bai L, Fu P, et al. The epidemiology of listeria monocytogenes in China［J］. Foodborne Pathogens and Disease, 2018, 15(8): 459-466.
［8］
Hassani S, Moosavy MH, Gharajalar SN, et al. High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk［J］. Scientific Reports, 2022, 12(1): 3878.
［9］
Moura A, Leclercq A, Vales G, et al. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France［J］. The Lancet Regional Health. Europe, 2024, 37: 100800.
［10］
Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd edition［EB/OL］. ［2024-09-19］. https://clsi.org/standards/products/microbiology/documents/m45/.
［11］
European Committee on Antimicrobial Susceptibility Testing. EUCAST: clinical breakpoints and dosing of antibiotics［EB/OL］. ［2024-09-19］. https://www.eucast.org/clinical_breakpoints.
［12］
李薇薇，郭云昌，占利，等．2017年中国即食食品中单核细胞增生李斯特菌的分子流行病学特征［J］．中华预防医学杂志, 2020, 54(2): 175-180.
Li WW, Guo YC, Zhan L, et al. Molecular epidemiology of Listeria monocytogenes isolated from ready-to-eat food in 2017 in China［J］. Chinese Journal of Preventive Medicine, 2020, 54(2): 175-180. (In Chinese)
［13］
国家市场监督管理总局，国家卫生健康委员会．GB 31607-2021 食品安全国家标准 散装即食食品中致病菌限量［S］．北京：中国标准出版社，2021．
The State Administration for Market Regulation, the National Health Commission. GB 31607-2021 National standards for food safety-Pathogenic bacteria limit in bulk ready-to-eat food［S］. Beijing: Standards Press of China, 2021. (In Chinese)
［14］
汪忆梦，张凤娟，于明明，等．2022年潍坊市肉与肉制品中单增李斯特菌污染状况分析［J］．食品安全导刊, 2023, (32): 13-15, 19.
Wang YM, Zhang FJ, Yu MM, et al. Analysis on contamination of Listeria monocytogenes in meat and meat products sold in Weifang in 2022［J］. China Food Safety Magazine, 2023, (32): 13-15, 19. (In Chinese)
［15］
林慧琳，叶玲清，叶海梅，等．2011—2021年福建省即食食品中单增李斯特菌的监测及菌株特征分析［J］．中国人兽共患病学报, 2023, 39(10): 941-950.
Lin HL, Ye LQ, Ye HM, et al. Monitoring of listeria monocytogenes in ready-to-eat foods and characterization of isolates in Fujian province from 2011 to 2021［J］. Chinese Journal of Zoonoses, 2023, 39(10): 941-950. (In Chinese)
［16］
Liu YT, Sun WX, Sun TM, et al. The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach［J］. International Journal of Food Microbiology, 2020, 312: 108358.
［17］
Bouymajane A, Rhazi filali F, Oulghazi S, et al. Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods［J］. Heliyon, 2021, 7(2): e06169.
［18］
Zhang YY, Dong SL, Chen HH, et al. Prevalence, genotypic characteristics and antibiotic resistance of listeria monocytogenes from retail foods in bulk in Zhejiang province, China［J］. Frontiers in Microbiology, 2019, 10: 1710.
［19］
蔡炯，陈潇，朱虹霖，等．成都市冷藏即食预包装熟肉制品中单核细胞增生李斯特菌定量检测及污染风险研究［J］．中国食品卫生杂志, 2024, 36(4): 426-432.
Cai J, Chen X, Zhu HL, et al. Research on quantitative and contamination level of Listeria monocytogenes from pre-packaged refrigerated ready-to-eat meat in Chengdu city［J］. Chinese Journal of Food Hygiene, 2024, 36(4): 426-432. (In Chinese)
［20］
Zhu L, Ji X, Wu Y, et al, Huang X. Molecular characterization of Listeria monocytogenes strains isolated from imported food in China from 14 countries/regions, 2003-2018［J］. Frontiers in Cellular and Infection Microbiology, 2023, 13: 1287564.
［21］
Gan L, Cao XL, Wang Y, et al. Carriage and potential long distance transmission of Listeria monocytogenes by migratory black-headed gulls in Dianchi Lake, Kunming［J］. Emerging Microbes & Infections, 2019, 8(1): 1195-1204.
［22］
Moura A, Leclercq A, Vales G, et al. Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France［J］. The Lancet Regional Health. Europe, 2023, 37: 100800.
2024年第51卷第22期
PDF下载
77
35
引用本文
BibTeX
文章信息
doi: 10.20043/j.cnki.MPM.202404454
  • 接收时间：2024-04-26
  • 首发时间：2026-03-20
  • 出版时间：2024-11-25
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2024-04-26
基金
广西自然科学基金项目(2018GXNSFAA050039)
作者信息
    广西壮族自治区疾病预防控制中心，广西　南宁　530028

通讯作者:

吕素玲，E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202404454
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持