首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1241025209508098937, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241025201983508979, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202501303, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1737129600000, receivedDateStr=2025-01-18, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1773813067080, onlineDateStr=2026-03-18, pubDate=1744214400000, pubDateStr=2025-04-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1773813067080, onlineIssueDateStr=2026-03-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1773813067080, creator=13701087609, updateTime=1773813067080, updator=13701087609, issue=Issue{id=1241025201983508979, tenantId=1146029695717560320, journalId=1227665162245664772, year='2025', volume='52', issue='7', pageStart='1153', pageEnd='1344', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1773813065285, creator=13701087609, updateTime=1773815493878, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1241035388320543403, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241025201983508979, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1241035388320543404, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1241025201983508979, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1311, endPage=1318, ext={EN=ArticleExt(id=1241025210179187611, articleId=1241025209508098937, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Study on the bacteriostatic effect and mechanism of methanol extract of Rosa sertata × Rosa rugosa, columnId=1228016572065837304, journalTitle=Modern Preventive Medicine, columnName=Experimental Technology and Applications, runingTitle=null, highlight=null, articleAbstract=
Objective

To elucidate antibacterial effects and mechanism of Rosa sertata×Rose rugosa methanol extraction (RME) against extended-spectrum β-lactamases producing Klebsiella pneumoniae (ESBL-KP).

Methods

Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of RME against various ESBL-KP strains were determined by micro broth dilution method. Impact of RME on the growth, cell membrane and extended-spectrum β-lactamases (ESBLs) activity of ESBL-KP was evaluated by growth and time-kill curves, scanning electron microscopy and nitrocefin hydrolysis test, seperately. Components of the extracts were analyzed using Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-Q-TOF MS).

Results

The MIC of RME was 1 mg/ml for standard strains and 8-32 mg/ml for ESBL-producing strains. Concentrations of RME at 1/4 MIC, 1/2 MIC, and MIC could inhibit bacterial growth, with higher concentrations showing stronger effects. For D141 strain, treatment with RME at 4MIC concentration for 4 hours resulted in cell membrane collapse, bacterial agglutination, and a 77.49% inhibition rate of ESBLs activity; no bacterial growth was observed after 24 hours of this treatment. Totally, 1 550 compounds were identified in RME, with quinic acid, tricoumaroyl spermidine, thioetheramide-PC, and kaempferol-3-O-rutinoside being the predominant components.

Conclusion

RME can inhibit and kill ESBL-KP through disrupting its cell membrane and inhibiting the enzymatic activity of ESBLs, which provided a scientific basis for further research on Rosa sertata × Rosa rugosa.

, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Qiu-hong XU, Lin-xuan LIAO, Qiu-rong HE, Xiao ZHANG, Li-ying YI, Jian WANG, Bao-chao ZHANG, Xin XU, Xiao-fang PEI), CN=ArticleExt(id=1241025212624466081, articleId=1241025209508098937, tenantId=1146029695717560320, journalId=1227665162245664772, language=CN, title=苦水玫瑰甲醇提取物对产超广谱β-内酰胺酶肺炎克雷伯菌的抑菌效果研究及机制初探, columnId=1228016572288135432, journalTitle=现代预防医学, columnName=实验技术及其应用, runingTitle=null, highlight=null, articleAbstract=
目的

探讨苦水玫瑰甲醇提取物（RME）对产超广谱β-内酰胺酶（ESBL）肺炎克雷伯菌（ESBL-KP）的抑菌效果及机制。

方法

采用微量肉汤稀释法、生长曲线和时间-杀菌曲线评估RME对不同来源ESBL-KP的体外抗菌活性；采用扫描电镜和头孢硝噻吩水解法测定RME对实验菌株细胞形态和ESBLs活性的影响；采用超高效液相色谱-四级杆-飞行时间质谱（UHPLC-Q-TOF MS）测定RME的化学成分。

结果

RME对标准菌株MIC为1 mg/ml，对产ESBL菌株MIC为8～32 mg/ml；1/4 MIC、1/2 MIC、MIC浓度的RME均能抑制受试菌生长，且浓度越高抑制作用越强；4 MIC浓度的RME作用受试菌株4 h，可见细胞膜塌陷、菌体黏连等改变，ESBLs活性抑制率达77.49%，作用24 h后未见细菌生长。RME中共检出1 550种化合物，其中奎宁酸、三香豆酰亚精胺和硫醚酰胺-PC、山奈酚-3-O-芸香糖苷是主要成分。

结论

RME对ESBL-KP具有良好的抑制和杀灭作用，可破坏其细胞膜、抑制ESBLs活性，RME对ESBL-KP的抑菌作用为苦水玫瑰的进一步研究和开发利用提供了重要的科学依据。

, correspAuthors=null, authorNote=null, correspAuthorsNote=
裴晓方，E-mail:
, copyrightStatement=本刊刊出的所有文章不代表中华预防医学会和本刊编委会的观点，除非特别声明。, copyrightOwner=中华预防医学会和四川大学华西公共卫生学院, extLink=null, articleAbsUrl=null, sourceXml=Hs397QLndGPayPBfU4HJLg==, magXml=TuAG7q04VWgk5yfT05gyAA==, pdfUrl=null, pdf=iPEbH7WqbwYysILY/h48iA==, pdfFileSize=1045818, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=x1C+6o6B+xtqsQF33OJ7SQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=7pSdt9Uu0RgzHQbSn2yjmw==, mapNumber=null, authorCompany=null, fund=null, authors=

徐秋红（1998—），女，硕士在读，研究方向：微生物，公众健康与检验

, authorsList=徐秋红, 廖琳萱, 何秋蓉, 张潇, 易丽颖, 王健, 张保超, 许欣, 裴晓方)}, authors=[Author(id=1241025214356713675, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025214486737109, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214356713675, language=EN, stringName=Qiu-hong XU, firstName=Qiu-hong, middleName=null, lastName=XU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025214579011805, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214356713675, language=CN, stringName=徐秋红, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio={"content":"

徐秋红（1998—），女，硕士在读，研究方向：微生物，公众健康与检验

"}, bioImg=null, bioContent=

徐秋红（1998—），女，硕士在读，研究方向：微生物，公众健康与检验

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025214667092194, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025214750978282, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214667092194, language=EN, stringName=Lin-xuan LIAO, firstName=Lin-xuan, middleName=null, lastName=LIAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025214839058676, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214667092194, language=CN, stringName=廖琳萱, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025214948110589, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025215048773895, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214948110589, language=EN, stringName=Qiu-rong HE, firstName=Qiu-rong, middleName=null, lastName=HE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025215153631504, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025214948110589, language=CN, stringName=何秋蓉, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025215304626458, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025215401095458, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025215304626458, language=EN, stringName=Xiao ZHANG, firstName=Xiao, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025215493370152, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025215304626458, language=CN, stringName=张潇, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025215615004980, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025215803748668, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025215615004980, language=EN, stringName=Li-ying YI, firstName=Li-ying, middleName=null, lastName=YI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025215921189184, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025215615004980, language=CN, stringName=易丽颖, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025216026046797, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025216122515800, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216026046797, language=EN, stringName=Jian WANG, firstName=Jian, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025216210596191, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216026046797, language=CN, stringName=王健, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025216286093669, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025216390951279, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216286093669, language=EN, stringName=Bao-chao ZHANG, firstName=Bao-chao, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025216487420279, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216286093669, language=CN, stringName=张保超, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025216583889281, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=7, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025216709718410, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216583889281, language=EN, stringName=Xin XU, firstName=Xin, middleName=null, lastName=XU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025216856519059, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025216583889281, language=CN, stringName=许欣, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])]), Author(id=1241025217024291235, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, orderNo=8, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=xxpeiscu@163.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1241025217158508970, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025217024291235, language=EN, stringName=Xiao-fang PEI, firstName=Xiao-fang, middleName=null, lastName=PEI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1241025217242395054, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, authorId=1241025217024291235, language=CN, stringName=裴晓方, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])])], keywords=[Keyword(id=1241025217380807097, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, orderNo=1, keyword=Extended-spectrum beta-lactamases), Keyword(id=1241025218865590720, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, orderNo=2, keyword=Klebsiella pneumoniae), Keyword(id=1241025218974642627, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, orderNo=3, keyword=Rosa sertata × Rosa rugosa), Keyword(id=1241025219121443273, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, orderNo=4, keyword=Antimicrobial activity), Keyword(id=1241025219234689487, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, orderNo=1, keyword=超广谱β-内酰胺酶), Keyword(id=1241025219356324310, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, orderNo=2, keyword=肺炎克雷伯菌), Keyword(id=1241025219465376215, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, orderNo=3, keyword=苦水玫瑰), Keyword(id=1241025219582816733, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, orderNo=4, keyword=抑菌)], refs=[Reference(id=1241025221545751096, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2024, volume=38, issue=10, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Ejaz H, Qamar MU, Farhana A, journalName=J Cli Lab Anal, refType=null, unstructuredReference=Ejaz H, Qamar MU, Farhana A, et al. The rising tide of antibiotic resistance: a study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant escherichia coli and klebsiella pneumoniae[J]. J Cli Lab Anal, 2024, 38(10): e25081., articleTitle=The rising tide of antibiotic resistance: a study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant escherichia coli and klebsiella pneumoniae, refAbstract=null), Reference(id=1241025221629637180, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2024, volume=14, issue=17, pageStart=2490, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=Mandujano-Hernández A, Martínez-Vázquez AV, Paz-González AD, journalName=Animals, refType=null, unstructuredReference=Mandujano-Hernández A, Martínez-Vázquez AV, Paz-González AD, et al. The global rise of ESBL-Producing escherichia coli in the livestock sector: a Five-Year overview[J]. Animals, 2024, 14(17):2490., articleTitle=The global rise of ESBL-Producing escherichia coli in the livestock sector: a Five-Year overview, refAbstract=null), Reference(id=1241025221734494786, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=11, issue=11, pageStart=2937, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=Husna A, Rahman MM, Badruzzaman ATM, journalName=Biomedicines, refType=null, unstructuredReference=Husna A, Rahman MM, Badruzzaman ATM, et al.Extended- Spectrum β-Lactamases (ESBL): challenges and opportunities[J]. Biomedicines, 2023, 11(11): 2937., articleTitle=Extended- Spectrum β-Lactamases (ESBL): challenges and opportunities, refAbstract=null), Reference(id=1241025221805797958, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=45, issue=2, pageStart=120, pageEnd=128, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=颜子曦, 李永慧, 孟德豪, journalName=北京林业大学学报, refType=null, unstructuredReference=颜子曦，李永慧，孟德豪，等.苦水玫瑰中49项营养及活性成分指标分析与评价[J].北京林业大学学报，2023，45（2）：120-128., articleTitle=苦水玫瑰中49项营养及活性成分指标分析与评价, refAbstract=null), Reference(id=1241025223273804361, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=45, issue=2, pageStart=120, pageEnd=128, url=null, language=null, rfNumber=[4], rfOrder=4, authorNames=Yan ZX, Li YH, Meng DH, journalName=Journal of Beijing Forestry University, refType=null, unstructuredReference=Yan ZX，Li YH，Meng DH， et al. Analysis and evaluation of 49 nutritional and active component indexes of Kushui rose (Rosa setate × R. rugosa)[J].Journal of Beijing Forestry University， 2023， 45(2): 120-128.(In Chinese), articleTitle=Analysis and evaluation of 49 nutritional and active component indexes of Kushui rose (Rosa setate × R. rugosa), refAbstract=null), Reference(id=1241025223361884748, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=6, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=5, authorNames=Wu MQ, Xu JY, Li W, journalName=Carbohydrate Polymer Technologies and Applications, refType=null, unstructuredReference=Wu MQ, Xu JY, Li W, et al. Surface morphology and action mechanism of macrophage immunostimulating activity of Rosa Setate x Rosa Rugosa polysaccharide WSRP-1b[J]. Carbohydrate Polymer Technologies and Applications, 2023, 6: 100339., articleTitle=Surface morphology and action mechanism of macrophage immunostimulating activity of Rosa Setate x Rosa Rugosa polysaccharide WSRP-1b, refAbstract=null), Reference(id=1241025223433187920, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=38, issue=12, pageStart=151, pageEnd=157, url=null, language=null, rfNumber=[6], rfOrder=6, authorNames=马金璞, 施生玲, 张钰璇, journalName=中国粮油学报, refType=null, unstructuredReference=马金璞，施生玲，张钰璇，等.超声微波协同萃取法提取兰州苦水玫瑰精油及抑菌活性的研究[J].中国粮油学报，2023，38（12）：151-157., articleTitle=超声微波协同萃取法提取兰州苦水玫瑰精油及抑菌活性的研究, refAbstract=null), Reference(id=1241025223521268308, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=38, issue=12, pageStart=151, pageEnd=157, url=null, language=null, rfNumber=[6], rfOrder=7, authorNames=Ma JP, Shi SL, Zhang YX, journalName=Journal of the Chinese Cereals and Oils Association, refType=null, unstructuredReference=Ma JP，Shi SL，Zhang YX， et al. Optimized extraction parameters of essential oil from Lanzhou kushui rose by ultrasonic microwave synergistic extraction method using RSM and its antibacterial activities[J]. Journal of the Chinese Cereals and Oils Association， 2023， 38(12): 151-157.(In Chinese), articleTitle=Optimized extraction parameters of essential oil from Lanzhou kushui rose by ultrasonic microwave synergistic extraction method using RSM and its antibacterial activities, refAbstract=null), Reference(id=1241025223626125911, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2022, volume=27, issue=15, pageStart=4971, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=8, authorNames=Mehta J, Utkarsh K, Fuloria S, journalName=Molecules, refType=null, unstructuredReference=Mehta J, Utkarsh K, Fuloria S, et al. Antibacterial potential of bacopa monnieri (L.) wettst. and its bioactive molecules against Uropathogens-An in silico study to identify potential Lead molecule(s) for the development of new drugs to treat urinary tract infections[J]. Molecules, 2022, 27(15): 4971., articleTitle=Antibacterial potential of bacopa monnieri (L.) wettst. and its bioactive molecules against Uropathogens-An in silico study to identify potential Lead molecule(s) for the development of new drugs to treat urinary tract infections, refAbstract=null), Reference(id=1241025223714206297, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=9, authorNames=CLSI, journalName=null, refType=null, unstructuredReference=CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically[EB/OL]. [2025-03-15]. https://www.semanticscholar.org/paper/Methods-for-dilution-antimicrobial-susceptibility-%3A-Wikler-Clinical/e418f7892e51569e2357e30ae0cd9a43454b127a., articleTitle=Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, refAbstract=null), Reference(id=1241025223823258204, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2025, volume=65, issue=2, pageStart=683, pageEnd=697, url=null, language=null, rfNumber=[9], rfOrder=10, authorNames=褚宽, 黄雷, 郑阿钦, journalName=微生物学报, refType=null, unstructuredReference=褚宽，黄雷，郑阿钦，等.甘胆口服液对副溶血弧菌的抑菌作用及转录组分析[J].微生物学报，2025，65（2）：683-697., articleTitle=甘胆口服液对副溶血弧菌的抑菌作用及转录组分析, refAbstract=null), Reference(id=1241025223911338591, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2025, volume=65, issue=2, pageStart=683, pageEnd=697, url=null, language=null, rfNumber=[9], rfOrder=11, authorNames=Chu K, Huang L, Zheng AQ, journalName=Acta Microbiologica Sinica, refType=null, unstructuredReference=Chu K，Huang L，Zheng AQ， et al. Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrio parahaemolyticus[J]. Acta Microbiologica Sinica， 2025， 65(2): 683-697.(In Chinese), articleTitle=Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrio parahaemolyticus, refAbstract=null), Reference(id=1241025223999418979, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=12, issue=1, pageStart=60, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=12, authorNames=Antoniadou M, Rozos G, Vaiou N, journalName=Microorganisms, refType=null, unstructuredReference=Antoniadou M, Rozos G, Vaiou N, et al. The in vitro assessment of antibacterial and antioxidant efficacy in Rosa damascena and hypericum perforatum extracts against pathogenic strains in the interplay of dental caries, oral health, and food microbiota[J].Microorganisms, 2023, 12(1): 60., articleTitle=The in vitro assessment of antibacterial and antioxidant efficacy in Rosa damascena and hypericum perforatum extracts against pathogenic strains in the interplay of dental caries, oral health, and food microbiota, refAbstract=null), Reference(id=1241025224087499369, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=54, issue=8, pageStart=2480, pageEnd=2487, url=null, language=null, rfNumber=[11], rfOrder=13, authorNames=蓝诗华, 吴小诗, 刘嘉鑫, journalName=中草药, refType=null, unstructuredReference=蓝诗华，吴小诗，刘嘉鑫，等.诃子水提物对球形马拉色菌及其生物被膜的抑制作用[J].中草药，2023，54（8）：2480-2487., articleTitle=诃子水提物对球形马拉色菌及其生物被膜的抑制作用, refAbstract=null), Reference(id=1241025224179774057, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=54, issue=8, pageStart=2480, pageEnd=2487, url=null, language=null, rfNumber=[11], rfOrder=14, authorNames=Lan SH, Wu XS, Liu JX, journalName=Chinese Traditional and Herbal Drugs, refType=null, unstructuredReference=Lan SH，Wu XS，Liu JX， et al. Inhibitory effect of water extract of Terminalia chebula on Malassezia globosa and biofilm[J]. Chinese Traditional and Herbal Drugs， 2023， 54(8): 2480-2487.(In Chinese), articleTitle=Inhibitory effect of water extract of Terminalia chebula on Malassezia globosa and biofilm, refAbstract=null), Reference(id=1241025224272048750, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=15, authorNames=苟玉虹, journalName=null, refType=null, unstructuredReference=苟玉虹.肉桂醛联合头孢曲松治疗耐药性沙门氏菌感染的药效学研究[D].雅安：四川农业大学，2022., articleTitle=肉桂醛联合头孢曲松治疗耐药性沙门氏菌感染的药效学研究, refAbstract=null), Reference(id=1241025224431432305, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=16, authorNames=Gou YH, journalName=null, refType=null, unstructuredReference=Gou YH. Pharmacodynamic study of cinnamaldehyde combined with ceftriaxone in the treatment of drug-resistant Salmonella infection[D].Yaan: Sichuan Agriculture University， 2022.(In Chinese), articleTitle=Pharmacodynamic study of cinnamaldehyde combined with ceftriaxone in the treatment of drug-resistant Salmonella infection, refAbstract=null), Reference(id=1241025224565650038, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2022, volume=34, issue=7, pageStart=1129, pageEnd=1142, url=null, language=null, rfNumber=[13], rfOrder=17, authorNames=杜少波, 陈晓文, 董志强, journalName=天然产物研究与开发, refType=null, unstructuredReference=杜少波，陈晓文，董志强，等.基于UPLC-Q-TOF-MS的苦水玫瑰醇提物化学成分分析[J].天然产物研究与开发，2022，34（7）：1129-1142., articleTitle=基于UPLC-Q-TOF-MS的苦水玫瑰醇提物化学成分分析, refAbstract=null), Reference(id=1241025224678896249, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2022, volume=34, issue=7, pageStart=1129, pageEnd=1142, url=null, language=null, rfNumber=[13], rfOrder=18, authorNames=Du SB, Chen XW, Dong ZQ, journalName=Natural Product Research and Development, refType=null, unstructuredReference=Du SB，Chen XW，Dong ZQ， et al. Analysis of chemical components in alcohol extract of Rosa sertata×Rosa rugosa based on UPLC-Q-TOF-MS[J]. Natural Product Research and Development， 2022， 34(7): 1129-1142.(In Chinese), articleTitle=Analysis of chemical components in alcohol extract of Rosa sertata×Rosa rugosa based on UPLC-Q-TOF-MS, refAbstract=null), Reference(id=1241025224771170938, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2020, volume=25, issue=6, pageStart=1365, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=19, authorNames=Cendrowski A, Kraśniewska K, Przybył JL, journalName=Molecules, refType=null, unstructuredReference=Cendrowski A, Kraśniewska K, Przybył JL, et al. Antibacterial and antioxidant activity of extracts from rose fruits(Rosa rugosa)[J].Molecules, 2020, 25(6): 1365., articleTitle=Antibacterial and antioxidant activity of extracts from rose fruits(Rosa rugosa), refAbstract=null), Reference(id=1241025224863445628, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=28, issue=8, pageStart=3613, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=20, authorNames=Hassan MM, Albogami B, Mwabvu T, journalName=Molecules, refType=null, unstructuredReference=Hassan MM, Albogami B, Mwabvu T, et al. The antibacterial activity of Rhazya stricta extracts against klebsiella pneumoniae isolated from Some Soil invertebrates at high altitudes[J]. Molecules,2023, 28(8): 3613., articleTitle=The antibacterial activity of Rhazya stricta extracts against klebsiella pneumoniae isolated from Some Soil invertebrates at high altitudes, refAbstract=null), Reference(id=1241025224964108927, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2021, volume=2021, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=21, authorNames=Golbarg H, Mehdipour moghaddam MJ, journalName=BioMed Research International, refType=null, unstructuredReference=Golbarg H, Mehdipour moghaddam MJ. Antibacterial potency of medicinal plants including artemisia annua and oxalis corniculata against Multi-Drug resistance E. coil[J]. BioMed Research International, 2021, 2021: 9981915., articleTitle=Antibacterial potency of medicinal plants including artemisia annua and oxalis corniculata against Multi-Drug resistance E. coil, refAbstract=null), Reference(id=1241025225047995007, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=196, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=22, authorNames=Yuan Y, Liu L, Guo L, journalName=Industrial Crops and Products, refType=null, unstructuredReference=Yuan Y, Liu L, Guo L, et al. Antibacterial mechanism of rose essential oil against Pseudomonas putida isolated from white Hypsizygus marmoreus at cellular and metabolic levels[J]. Industrial Crops and Products, 2023, 196: 116523., articleTitle=Antibacterial mechanism of rose essential oil against Pseudomonas putida isolated from white Hypsizygus marmoreus at cellular and metabolic levels, refAbstract=null), Reference(id=1241025225127686785, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2017, volume=8, issue=3, pageStart=83, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=23, authorNames=Islam MS, Aryasomayajula A, Selvaganapathy PR, journalName=Micromachines, refType=null, unstructuredReference=Islam MS, Aryasomayajula A, Selvaganapathy PR. A review on macroscale and microscale cell lysis methods[J]. Micromachines,2017, 8(3): 83., articleTitle=A review on macroscale and microscale cell lysis methods, refAbstract=null), Reference(id=1241025225232544385, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=16, issue=1, pageStart=159, pageEnd=162, url=null, language=null, rfNumber=[19], rfOrder=24, authorNames=Jassim SA, Hassan MH, journalName=Res J Phar Tech, refType=null, unstructuredReference=Jassim SA, Hassan MH. Neem leaf extract as a Potential antibiofilm and anti ESBLS agent for K.pneumoniae[J]. Res J Phar Tech, 2023,16(1): 159-162., articleTitle=Neem leaf extract as a Potential antibiofilm and anti ESBLS agent for K.pneumoniae, refAbstract=null), Reference(id=1241025225366762116, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2023, volume=17, issue=2, pageStart=247, pageEnd=252, url=null, language=null, rfNumber=[20], rfOrder=25, authorNames=Peng Z, Ma YY, Peng JZ, journalName=Neurochemical Journal, refType=null, unstructuredReference=Peng Z, Ma YY, Peng JZ, et al. Kaempferol-3-O-Rutinoside:A natural flavonoid glycosides with multifaceted therapeutic potential[J]. Neurochemical Journal, 2023, 17(2): 247-252., articleTitle=Kaempferol-3-O-Rutinoside:A natural flavonoid glycosides with multifaceted therapeutic potential, refAbstract=null), Reference(id=1241025225454842500, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, doi=null, pmid=null, pmcid=null, year=2017, volume=110, issue=null, pageStart=390, pageEnd=398, url=null, language=null, rfNumber=[21], rfOrder=26, authorNames=Kordbacheh H, Eftekhar F, Ebrahimi SN, journalName=Microbial Pathogenesis, refType=null, unstructuredReference=Kordbacheh H, Eftekhar F, Ebrahimi SN. Anti-quorum sensing activity of Pistacia atlantica against Pseudomonas aeruginosa PAO1 and identification of its bioactive compounds[J]. Microbial Pathogenesis, 2017, 110: 390-398., articleTitle=Anti-quorum sensing activity of Pistacia atlantica against Pseudomonas aeruginosa PAO1 and identification of its bioactive compounds, refAbstract=null)], funds=[Fund(id=1241025221445087796, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, awardId=2022ZDZX0017, language=CN, fundingSource=四川省“十四五”生命健康重大科技项目“重大传染病监测预警与应对(2022ZDZX0017), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1241025212842569917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, xref=null, ext=[AuthorCompanyExt(id=1241025212850958526, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China), AuthorCompanyExt(id=1241025212871930050, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, companyId=1241025212842569917, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=四川大学华西公共卫生学院/华西第四医院，四川 成都 610041)])], figs=[ArticleFig(id=1241025219700257253, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Figure 1, caption=Growth curves of RME against ATCC 13883, H533, and D141, figureFileSmall=Xv7SLFCTP6RIR/DZDhfVMQ==, figureFileBig=x1C+6o6B+xtqsQF33OJ7SQ==, tableContent=null), ArticleFig(id=1241025219771560428, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=图1, caption=RME对ATCC 13883、H533和D141的生长曲线, figureFileSmall=Xv7SLFCTP6RIR/DZDhfVMQ==, figureFileBig=x1C+6o6B+xtqsQF33OJ7SQ==, tableContent=null), ArticleFig(id=1241025220081938936, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Figure 2, caption=The time-kill curves of RME against ATCC 13883,H533, and D141

Note: The dotted line indicates a 99.99% reduction in the number of bacteria from the initial culture.

, figureFileSmall=nwj2SEpu08CcQZmML0lb7w==, figureFileBig=LoC6az51MuH0fXibJG+ibQ==, tableContent=null), ArticleFig(id=1241025220241322492, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=图2, caption=RME对ATCC 13883、H533和D141的时间杀菌曲线

注：虚线表示细菌数量比初始培养减少了99.99%。

, figureFileSmall=nwj2SEpu08CcQZmML0lb7w==, figureFileBig=LoC6az51MuH0fXibJG+ibQ==, tableContent=null), ArticleFig(id=1241025220362957312, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Figure 3, caption=Scanning electron microscopy results of RME-treated D141 strain

Note: A and C are RME-treated groups; B and D are negative control groups. Red arrows indicate bacterial crumpling; yellow arrows indicate bacterial adhesion with blurred cell membrane boundaries; red circles indicate cell membrane collapse.

, figureFileSmall=BRWNDc+Prl/Xg/xrka1W4Q==, figureFileBig=Uzj0/kgfDuxn/Fp0oMF8Mw==, tableContent=null), ArticleFig(id=1241025220446843398, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=图3, caption=RME处理D141菌株的扫描电微镜结果

注：图A、C为RME处理组；图B、D为阴性对照；红色箭头表示细菌皱缩；黄色箭头表示细菌粘连，细胞膜边界模糊；红色圆圈表示细胞膜塌陷。

, figureFileSmall=BRWNDc+Prl/Xg/xrka1W4Q==, figureFileBig=Uzj0/kgfDuxn/Fp0oMF8Mw==, tableContent=null), ArticleFig(id=1241025220547506697, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Figure 4, caption=Inhibitory effect of RME on the activity of ESBLs in D141

Note: * indicates P<0.05 for the 32 mg/ml group compared to the 0 mg/ml group.

, figureFileSmall=0tmIWpmPzK1+7jAxHxPJpg==, figureFileBig=jlMN/rfCMD35yGouqKSVLQ==, tableContent=null), ArticleFig(id=1241025220631392781, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=图4, caption=RME对D141 ESBLs活性的影响

注：与对照组相比，*P<0.05。

, figureFileSmall=0tmIWpmPzK1+7jAxHxPJpg==, figureFileBig=jlMN/rfCMD35yGouqKSVLQ==, tableContent=null), ArticleFig(id=1241025220706890258, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Figure 5, caption=Total ion flow chromatogram of RME

Note: A represent positive ion mode, B represent negative ion mode; red, blue and pink lines represent the three parallel extracts of RME, respectively.

, figureFileSmall=m3iMsazmILcUTHlWqQ/Ofg==, figureFileBig=8lORl3/Dj+yULyoQhwkk4A==, tableContent=null), ArticleFig(id=1241025220782387734, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=图5, caption=RME的总离子流图

注：图A为正离子模式；图B为负离子模式；红色、蓝色、粉色线条分别代表RME的三次平行提取物。

, figureFileSmall=m3iMsazmILcUTHlWqQ/Ofg==, figureFileBig=8lORl3/Dj+yULyoQhwkk4A==, tableContent=null), ArticleFig(id=1241025220857885211, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Table 1, caption=

Number, origin and resistant profiles of the strains used in the study

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株来源耐药性多重耐药ESBL阳性
D141犬GEN，AMK，CIP，EN，MEM，CL，CTX，FEP，AMP，ATM，AMX/CA，SXT，TE是是
D100犬GEN，AMK，CIP，EN，CL，CTX，CAZ，AMP，ATM，AMX/CA，SXT，TE，C是是
D90-1犬GEN，AMK，CIP，CTX，AMP，AMX/CA，SXT，TE，C是是
D90-3犬GEN，AMK，CIP，CL，CTX，AMP，AMX/CA，SXT，TE，C是是
D142犬GEN，CIP，CL，CTX，AMP，ATM，SXT是是
H533人CIP，EN，CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H519人CIP，EN，CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H285人CL，CTX，CAZ，AMP否是
H554人CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT否是
H542人CIP，EN，CL，CTX，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H831人CL，CTX，FEP，AMP，AMX/CA，SXT否是
), ArticleFig(id=1241025220937576991, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=表1, caption=

实验所用菌株编号、来源及耐药谱

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株来源耐药性多重耐药ESBL阳性
D141犬GEN，AMK，CIP，EN，MEM，CL，CTX，FEP，AMP，ATM，AMX/CA，SXT，TE是是
D100犬GEN，AMK，CIP，EN，CL，CTX，CAZ，AMP，ATM，AMX/CA，SXT，TE，C是是
D90-1犬GEN，AMK，CIP，CTX，AMP，AMX/CA，SXT，TE，C是是
D90-3犬GEN，AMK，CIP，CL，CTX，AMP，AMX/CA，SXT，TE，C是是
D142犬GEN，CIP，CL，CTX，AMP，ATM，SXT是是
H533人CIP，EN，CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H519人CIP，EN，CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H285人CL，CTX，CAZ，AMP否是
H554人CL，CTX，CAZ，FEP，AMP，ATM，AMX/CA，SXT否是
H542人CIP，EN，CL，CTX，FEP，AMP，ATM，AMX/CA，SXT，TE，C是是
H831人CL，CTX，FEP，AMP，AMX/CA，SXT否是
), ArticleFig(id=1241025221038240291, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Table 2, caption=

MIC nad MBC of RME against ESBL-KP (mg/mL)

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株MICMBC
ATCC 1388314
D141816
D10016>32
D90-31632
D90-116>32
D1428>32
H533816
H519832
H28588
H554832
H5423232
H83116>32
), ArticleFig(id=1241025221130514983, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=表2, caption=

RME对ESBL-KP的MIC和MBC（mg/mL）

, figureFileSmall=null, figureFileBig=null, tableContent=
菌株MICMBC
ATCC 1388314
D141816
D10016>32
D90-31632
D90-116>32
D1428>32
H533816
H519832
H28588
H554832
H5423232
H83116>32
), ArticleFig(id=1241025221231178281, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=EN, label=Table 3, caption=

The top ten components in RME

, figureFileSmall=null, figureFileBig=null, tableContent=
序号名称大类子类rt（s）m/zPeak area（106）
1Quinic acid
（奎宁酸）		Organooxygen compounds
（有机含氧化合物）		Alcohols and polyols
（醇和多元醇）		342.155 5191.057 6339.18
2Tricoumaroyl spermidine
（三香豆酰亚精胺）		Cinnamic acids and derivatives
（肉桂酸及其衍生物）		Hydroxycinnamic acids and derivatives
（羟基肉桂酸及其衍生物）		99.842 0582.261 3635.86
3Thioetheramide-PC
（硫醚酰胺-PC）		——142.207 0780.551 6522.22
4Kaempferol 3-O-rutinoside
（山奈酚-3-O-芸香糖苷）		Flavonoids（黄酮类）Flavonoid glycosides
（黄酮苷）		127.735 5593.129 2418.52
5Pheophorbide a
（脱镁叶绿酸盐A）		Tetrapyrroles and derivatives
（四吡咯类化合物及其衍生物）		Chlorins（氯叶绿素）64.033 0593.273 6015.74
6Testosterone
（睾酮）		Steroids and steroid derivatives
（类固醇及其衍生物）		Androstane steroids
（雄甾烷类固醇）		117.262 5287.222 5013.02
7PC(16:0/16:0)Glycerophospholipids
（甘油磷酸脂质）		Glycerophosphocholines
（甘油磷酸胆碱）		144.606 5756.551 1012.38
8Ginsenoside f3
（人参皂苷F3）		Prenol lipids
（萜烯类脂质）		Triterpenoids
（三萜类）		64.769 0815.499 3712.25
9Acetylvalerenolic acid
（乙酰氧基缬草烯酸 ）		——103.640 5329.123 0310.71
102h-pyrrole,2-(5,5-dimethyl-2-
oxido-1,3,2-dioxaphosphorinan-2-
yl)-3,4-dihydro-2-methyl-, 1-oxide		Organic phosphonic acids and
derivatives
（有机膦酸及其衍生物）		—416.185 0248.112 129.61
), ArticleFig(id=1241025221336035888, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1241025209508098937, language=CN, label=表3, caption=

RME前十种成分

, figureFileSmall=null, figureFileBig=null, tableContent=
序号名称大类子类rt（s）m/zPeak area（106）
1Quinic acid
（奎宁酸）		Organooxygen compounds
（有机含氧化合物）		Alcohols and polyols
（醇和多元醇）		342.155 5191.057 6339.18
2Tricoumaroyl spermidine
（三香豆酰亚精胺）		Cinnamic acids and derivatives
（肉桂酸及其衍生物）		Hydroxycinnamic acids and derivatives
（羟基肉桂酸及其衍生物）		99.842 0582.261 3635.86
3Thioetheramide-PC
（硫醚酰胺-PC）		——142.207 0780.551 6522.22
4Kaempferol 3-O-rutinoside
（山奈酚-3-O-芸香糖苷）		Flavonoids（黄酮类）Flavonoid glycosides
（黄酮苷）		127.735 5593.129 2418.52
5Pheophorbide a
（脱镁叶绿酸盐A）		Tetrapyrroles and derivatives
（四吡咯类化合物及其衍生物）		Chlorins（氯叶绿素）64.033 0593.273 6015.74
6Testosterone
（睾酮）		Steroids and steroid derivatives
（类固醇及其衍生物）		Androstane steroids
（雄甾烷类固醇）		117.262 5287.222 5013.02
7PC(16:0/16:0)Glycerophospholipids
（甘油磷酸脂质）		Glycerophosphocholines
（甘油磷酸胆碱）		144.606 5756.551 1012.38
8Ginsenoside f3
（人参皂苷F3）		Prenol lipids
（萜烯类脂质）		Triterpenoids
（三萜类）		64.769 0815.499 3712.25
9Acetylvalerenolic acid
（乙酰氧基缬草烯酸 ）		——103.640 5329.123 0310.71
102h-pyrrole,2-(5,5-dimethyl-2-
oxido-1,3,2-dioxaphosphorinan-2-
yl)-3,4-dihydro-2-methyl-, 1-oxide		Organic phosphonic acids and
derivatives
（有机膦酸及其衍生物）		—416.185 0248.112 129.61
)], attaches=null, journal=Journal(id=1227664546253402114, delFlag=0, nameCn=现代预防医学, nameEn=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, issn=1003-8507, eissn=null, cn=51-1365/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=IeiuPXEZi6AA+k0VfvoiOQ==, journalPrice=null, startedYear=null, abbrevIsoEn=Modern Preventive Medicine, journalRemark=null, publicationField=null, createdTime=1770627636734, updatedTime=1770628902248, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=M, firstLetterEn=M, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=IeiuPXEZi6AA+k0VfvoiOQ==, picEn=/9iTl8/ndms4tBz1fL28Pg==, jcr=null, cjcr=null, exts=[JournalExt(id=1227669854342280188, language=CN, name=现代预防医学, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902278, updatedTime=1770628902278, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1227669854396806141, language=EN, name=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902291, updatedTime=1770628902291, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1227665162245664772, websiteList=[Website(id=1227687234141352800, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/CN, language=CN, createTime=1770633045945, createBy=18614031015, updateTime=1770633090526, updateBy=18614031015, name=现代预防医学-中文, tplId=1146099689490845704, title=现代预防医学, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687735088051072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=articleTextType, value=kx, createTime=1770633165380, updateTime=1770633165380, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735071273853, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=banner, value=null, createTime=1770633165376, updateTime=1770633165376, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735113216899, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=grayFlag, value=0, createTime=1770633165386, updateTime=1770633165386, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735062885244, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633165374, updateTime=1770633165374, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735125799813, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=minRunFlag, value=0, createTime=1770633165389, updateTime=1770633165389, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735083856767, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic, createTime=1770633165379, updateTime=1770633165379, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735121605508, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=silenceFlag, value=0, createTime=1770633165388, updateTime=1770633165388, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735079662462, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1770633165378, updateTime=1770633165378, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735096439681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeColor, value=null, createTime=1770633165382, updateTime=1770633165382, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735104828290, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeStyle, value=null, createTime=1770633165384, updateTime=1770633165384, creator=18614031015, updator=18614031015)]), Website(id=1227687234338485094, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/EN, language=EN, createTime=1770633045992, createBy=18614031015, updateTime=1770633115374, updateBy=18614031015, name=现代预防医学-英文, tplId=1146101810881728533, title=Modern Preventive Medicine, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687709129507332, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=articleTextType, value=kx, createTime=1770633159191, updateTime=1770633159191, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709108535809, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=banner, value=null, createTime=1770633159186, updateTime=1770633159186, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709167256071, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=grayFlag, value=0, createTime=1770633159200, updateTime=1770633159200, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709095952896, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633159183, updateTime=1770633159183, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709179838985, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=minRunFlag, value=0, createTime=1770633159203, updateTime=1770633159203, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709121118723, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic, createTime=1770633159189, updateTime=1770633159189, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709171450376, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=silenceFlag, value=0, createTime=1770633159201, updateTime=1770633159201, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709112730114, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1770633159187, updateTime=1770633159187, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709133701637, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeColor, value=null, createTime=1770633159192, updateTime=1770633159192, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709154673158, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeStyle, value=null, createTime=1770633159197, updateTime=1770633159197, creator=18614031015, updator=18614031015)])], journalTitle=现代预防医学, weixinUrl=null, journalUrl=http://xdyfyxzz.paperopen.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Modern Preventive Medicine, journalPhotoCn=IeiuPXEZi6AA+k0VfvoiOQ==, journalPhotoEn=/9iTl8/ndms4tBz1fL28Pg==, journalFirstLetter=M, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202501303, detailUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/10.20043/j.cnki.MPM.202501303, pdfUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/PDF/10.20043/j.cnki.MPM.202501303, pdfUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/PDF/10.20043/j.cnki.MPM.202501303, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
苦水玫瑰甲醇提取物对产超广谱β-内酰胺酶肺炎克雷伯菌的抑菌效果研究及机制初探
收藏切换
PDF下载
徐秋红 , 廖琳萱 , 何秋蓉 , 张潇 , 易丽颖 , 王健 , 张保超 , 许欣 , 裴晓方
现代预防医学 | 实验技术及其应用 2025,52(7): 1311-1318
收起
收藏切换
现代预防医学 | 实验技术及其应用 2025, 52(7): 1311-1318
苦水玫瑰甲醇提取物对产超广谱β-内酰胺酶肺炎克雷伯菌的抑菌效果研究及机制初探
全屏
徐秋红, 廖琳萱, 何秋蓉, 张潇, 易丽颖, 王健, 张保超, 许欣, 裴晓方
作者信息
  • 四川大学华西公共卫生学院/华西第四医院，四川 成都 610041

    • 徐秋红（1998—），女，硕士在读，研究方向：微生物，公众健康与检验

通讯作者:

裴晓方，E-mail:
Study on the bacteriostatic effect and mechanism of methanol extract of Rosa sertata × Rosa rugosa
Qiu-hong XU, Lin-xuan LIAO, Qiu-rong HE, Xiao ZHANG, Li-ying YI, Jian WANG, Bao-chao ZHANG, Xin XU, Xiao-fang PEI
Affiliations
  • West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China
出版时间: 2025-04-10 doi: 10.20043/j.cnki.MPM.202501303
文章导航
收藏切换
摘要
收起
目的

探讨苦水玫瑰甲醇提取物（RME）对产超广谱β-内酰胺酶（ESBL）肺炎克雷伯菌（ESBL-KP）的抑菌效果及机制。

方法

采用微量肉汤稀释法、生长曲线和时间-杀菌曲线评估RME对不同来源ESBL-KP的体外抗菌活性；采用扫描电镜和头孢硝噻吩水解法测定RME对实验菌株细胞形态和ESBLs活性的影响；采用超高效液相色谱-四级杆-飞行时间质谱（UHPLC-Q-TOF MS）测定RME的化学成分。

结果

RME对标准菌株MIC为1 mg/ml，对产ESBL菌株MIC为8～32 mg/ml；1/4 MIC、1/2 MIC、MIC浓度的RME均能抑制受试菌生长，且浓度越高抑制作用越强；4 MIC浓度的RME作用受试菌株4 h，可见细胞膜塌陷、菌体黏连等改变，ESBLs活性抑制率达77.49%，作用24 h后未见细菌生长。RME中共检出1 550种化合物，其中奎宁酸、三香豆酰亚精胺和硫醚酰胺-PC、山奈酚-3-O-芸香糖苷是主要成分。

结论

RME对ESBL-KP具有良好的抑制和杀灭作用，可破坏其细胞膜、抑制ESBLs活性，RME对ESBL-KP的抑菌作用为苦水玫瑰的进一步研究和开发利用提供了重要的科学依据。

超广谱β-内酰胺酶  /  肺炎克雷伯菌  /  苦水玫瑰  /  抑菌
Objective

To elucidate antibacterial effects and mechanism of Rosa sertata×Rose rugosa methanol extraction (RME) against extended-spectrum β-lactamases producing Klebsiella pneumoniae (ESBL-KP).

Methods

Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of RME against various ESBL-KP strains were determined by micro broth dilution method. Impact of RME on the growth, cell membrane and extended-spectrum β-lactamases (ESBLs) activity of ESBL-KP was evaluated by growth and time-kill curves, scanning electron microscopy and nitrocefin hydrolysis test, seperately. Components of the extracts were analyzed using Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-Q-TOF MS).

Results

The MIC of RME was 1 mg/ml for standard strains and 8-32 mg/ml for ESBL-producing strains. Concentrations of RME at 1/4 MIC, 1/2 MIC, and MIC could inhibit bacterial growth, with higher concentrations showing stronger effects. For D141 strain, treatment with RME at 4MIC concentration for 4 hours resulted in cell membrane collapse, bacterial agglutination, and a 77.49% inhibition rate of ESBLs activity; no bacterial growth was observed after 24 hours of this treatment. Totally, 1 550 compounds were identified in RME, with quinic acid, tricoumaroyl spermidine, thioetheramide-PC, and kaempferol-3-O-rutinoside being the predominant components.

Conclusion

RME can inhibit and kill ESBL-KP through disrupting its cell membrane and inhibiting the enzymatic activity of ESBLs, which provided a scientific basis for further research on Rosa sertata × Rosa rugosa.

Extended-spectrum beta-lactamases  /  Klebsiella pneumoniae  /  Rosa sertata × Rosa rugosa  /  Antimicrobial activity
徐秋红, 廖琳萱, 何秋蓉, 张潇, 易丽颖, 王健, 张保超, 许欣, 裴晓方. 苦水玫瑰甲醇提取物对产超广谱β-内酰胺酶肺炎克雷伯菌的抑菌效果研究及机制初探. 现代预防医学, 2025 , 52 (7) : 1311 -1318 . DOI: 10.20043/j.cnki.MPM.202501303
Qiu-hong XU, Lin-xuan LIAO, Qiu-rong HE, Xiao ZHANG, Li-ying YI, Jian WANG, Bao-chao ZHANG, Xin XU, Xiao-fang PEI. Study on the bacteriostatic effect and mechanism of methanol extract of Rosa sertata × Rosa rugosa[J]. Modern Preventive Medicine, 2025 , 52 (7) : 1311 -1318 . DOI: 10.20043/j.cnki.MPM.202501303
正文
收起
产超广谱β-内酰胺酶肺炎克雷伯菌（extendedspectrum β-lactamases producing Klebsiella pneumoniae，ESBL-KP）由于耐多药，如对哌拉西林和头孢吡肟耐药率均为100%，对诺氟沙星、环丙沙星、妥布霉素的耐药率分别为83.9%、66.9%、50.9%[1]，常导致患者严重感染和多种并发症，极大增加了医疗负担，甚至危及生命。超广谱β-内酰胺酶（ESBLs）是ESBL-KP乃至革兰阴性菌的常见耐药机制之一，能够水解氧亚氨基-头孢菌素（第3代和第4代头孢菌素）和单环酰胺类药物，主要有CTX-M、TEM和SHV三类，每类酶的变体均已超过220种[2]。ESBLs由质粒基因编码，通过细菌接合等方式可在细菌间传播[3]，是抗生素耐药性的重要来源，世界卫生组织已将ESBL-KP纳入开发新抗菌剂的优先病原体。
苦水玫瑰（Rosa sertata × Rosa rugosa）为中国传统的药食同源植物，拥有超200年的栽培历史，被广泛应用于食品、茶、药等领域，其功效已引起关注。研究者发现苦水玫瑰富含人体必须营养物质和具有抗氧化作用的黄酮类、多酚类化合物，并且分离出的多糖WSRP-1b具有免疫调节作用[4-5]。此外，苦水玫瑰还具有抗菌活性，马金璞等[6]发现苦水玫瑰精油对大肠杆菌和金黄色葡萄球菌具有抑制作用。然而，有关苦水玫瑰针对ESBL-KP的抑菌作用尚无报道。因此，本研究以苦水玫瑰为研究对象，选择常用的甲醇作为溶剂，测定其甲醇提取物（Rosa sertata × Rosa rugosa methanol extract，RME）对产ESBL-KP的抗菌活性，并从细胞形态和ESBLs活性两方面初步探讨作用机制，并检测其化学成分，为苦水玫瑰健康效应研究和开发利用提供科学依据。
1　材料与方法
收起
1.1　材料
本实验所用苦水玫瑰干花蕾购自淘宝淮仁堂旗舰店。
ATCC 13883为肺炎克雷伯菌标准菌株，由四川大学华西公共卫生学院实验中心保存。其余菌株由本课题组从成都某三甲医院临床标本或宠物医院犬肛拭子等标本分离获得，均为耐药菌株，菌株信息见表1。
Mueller-Hinton（MH）培养基、阳离子调节肉汤（cationadjusted mueller-hinton broth， CAMHB）、Nutrient Agar（NA）培养基（青岛海博生物技术有限公司），刃天青（上海麦克林生化科技股份有限公司），甲醇（成都市科隆化学品有限公司），二甲基亚砜（dimethyl sulfoxide，DMSO，美国AbMole Bioscience公司），头孢硝噻吩（上海阿拉丁生化科技股份有限公司）。
1.2　方法
1.2.1　RME的制备
称取粉碎后的植物25 g置于锥形瓶，加入250 ml甲醇，25℃振荡提取72 h，提取液抽滤去除残渣后减压旋蒸，获得的结晶用25 ml DMSO溶解制得1 g/ml（以生药计）的RME，平行提取三次，分装后-80℃避光保存，用于后续实验[7]。
1.2.2　MIC和MBC测定
参照美国临床实验室标准化协会（CLSI）方案[8]采用微量肉汤稀释法测定RME对实验菌株的最小抑菌浓度（minimum inhibition concentrations，MIC），RME终浓度为0.25～32 mg/ml，菌悬液终浓度约为5×105 CFU/ml，CAMHB为阴性对照，含菌液的CAMHB为阳性对照，含等浓度DMSO的CAMHB为溶剂对照，每组3个复孔。37℃培养18～20 h后，每孔加50 μl 0.03%刃天青，孵育后基于颜色变化确定MIC；将MIC、2 MIC、4 MIC对应孔内100 μl液体涂布接种于MH平板，37℃培养18～20h后计数，以杀死99.99%细菌的最低浓度为最小杀菌浓度（minimum bactericidal concentration，MBC），实验重复三次[8]。
1.2.3　生长曲线测定
实验组RME设置MIC、1/2 MIC、1/4 MIC三个浓度，用MH肉汤稀释RME后，各浓度取4.9 ml分别与100 μl 0.5麦氏浊度实验菌株悬液在六孔板中混匀，细菌终浓度约106～107 CFU/ml，含等浓度DMSO的MH肉汤加菌液作阴性对照，37℃孵育24 h，每2 h测OD600值，绘制生长曲线，实验重复三次[9]。
1.2.4　时间-杀菌曲线实验
实验采用4 MIC浓度的RME，用MH肉汤稀释RME后，各浓度取4.9 ml分别与100 μl 0.5麦氏浊度实验菌株悬液混匀，细菌终浓度约106～107 CFU/ml，含等浓度DMSO的MH肉汤加菌液作阴性对照，37℃孵育24 h，于0、2、4、6、8、10、12和24 h吸取六孔板中每孔100 μl液体，用生理盐水10倍系列稀释后分别滴加并涂布于MH平板表面，次日计数菌落数，绘制时间-杀菌曲线，实验重复三次[10]。
1.2.5　扫描电镜观察细菌形态
64 mg/ml的RME与OD600约0.45的对数生长期D141菌悬液各1 ml加入六孔板（RME终浓度为4MIC），37℃培养4 h后收集所有液体于4℃、5 000 r/min离心5 min，收集菌体沉淀，加入1 ml 2.5%戊二醛固定，同时设阴性对照（与处理组等浓度DMSO的MH肉汤加菌液），样品于4℃条件下送至成都里来生物科技有限公司进行扫描电镜观察[11]。
1.2.6　头孢硝噻吩水解法测定ESBLs活性
64 mg/ml的RME和OD600约0.45的对数生长期D141菌液各1 ml加入六孔板（RME终浓度为4MIC），37℃振荡培养4 h后离心收集菌体沉淀，用PBS缓冲液洗涤后于冰水浴超声破碎15 min（超声时间和间隔各5 s，振幅35%），离心收集上清，获得ESBLs粗提液，测定其蛋白浓度备用。于96孔板各孔分别加90 μl PBS和100 μl ESBLs酶粗提取液，再加10 μl头孢硝噻吩溶液（0.5 mg/ml），37℃孵育25 min后测定OD490，同时设阴性对照（含与处理组等浓度DMSO的MH肉汤加菌液）和空白对照（MH肉汤）[12]。酶活性抑制率计算公式如下：
1.2.7　UHPLC-Q-TOF MS测定RME成分
采用Agilent 1290 Infinity LC超高效液相色谱系统（UHPLC）HILIC对RME进行分离和检测。色谱条件：水+25 mM乙酸铵+25mM氨水（A）和乙腈（B）作为流动相，梯度洗脱，体积流量0.5 ml/min，进样量2 μl，柱温25℃。采用AB Triple TOF 6600质谱仪（AB SCIEX）进行质谱分析，分别采用电喷雾电离（ESI）正离子和负离子模式进行检测。数据分析通过XCMS软件进行，包括峰识别、定量分析和结构鉴定[13]。
1.2.8　统计分析方法
使用Graphpad Prism 9.5.1软件进行统计分析与绘图。当数据符合正态分布且方差齐性时，运用单因素方差分析，并用Tukey多重比较法进行组间两两比较；数据仅满足正态分布时，使用Welch ANOVA进行比较，并采用Games-Howell test进行两两比较；两个条件均不满足时，使用Kruskal-Wallis test进行比较。检验水准α=0.05。
2　结果
收起
2.1　RME的MIC和MBC结果
表2为RME对各菌株的MIC和MBC。RME对ATCC 13883的MIC及MBC值均最小，分别为1和4 mg/ml；RME对各ESBL-KP菌株的MIC范围在8～32 mg/ml，提示RME对ESBL-KP具有广泛的抑制作用。
2.2　RME对实验菌株生长趋势的影响
不同浓度RME处理ATCC 13883、H533、D141的生长曲线如图1所示，各菌株实验组OD600值均低于对照组，其中MIC组OD600始终保持在初始水平，表明1/4MIC、1/2MIC、MIC浓度的RME均能抑制受试菌株生长。
2.3　RME对实验菌株的时间-杀菌曲线
使用RME处理ATCC 13883、H533、D141的时间-杀菌曲线见图2，4MIC浓度的RME作用10 h可使ATCC 13883和D141活菌数量减少4 Log10 CFU/ml，作用24 h可使H533和D141活菌数量下降>7 Log10 CFU/ml。
2.4　RME对D141菌株形态的影响
扫描电镜下可见4MIC浓度的RME处理D141菌株4 h后大量细菌表面皱缩，菌体黏连、边界模糊，细胞膜塌陷；对照组细菌呈杆状，形态饱满，菌体边界清晰。见图3。
2.5　RME对D141菌株ESBLs活性的影响
RME浓度越高，对ESBLs活性的影响越大，32 mg/ml（4MIC）浓度的RME对ESBLs活性的抑制率达77.49%（P<0.05）。见图4。
2.6　RME成分分析
RME正离子模式检出891种化合物，负离子模式检出659种化合物，共检出1 550种，其中脂质和类脂质分子最多（425种），其次为有机酸及其衍生物（198种）、苯丙素和聚酮化合物（190种），总离子流图见图5。检出化合物中含量前十的成分见表3，其中奎尼酸、三香豆酰亚精胺、硫醚酰胺-PC和山奈酚-3-O-芸香糖苷为含量前四的化合物。
3　讨论
收起
ESBL-KP的耐药性给全球公共卫生带来巨大挑战，迫切需要寻找新的抗菌剂来应对，而天然植物因富含多种生物活性物质成为解决此问题的潜在策略。本研究基于课题组多年对植物提取物研究的积累，选择苦水玫瑰研究RME对ESBL-KP的抑制作用。结果显示，RME对不同耐药表型的ESBL-KP菌株具有广泛的抑制作用。RME对标准菌株的抑制能力（MIC=1 mg/ml）强于玫瑰（Rosa rugosa）果实提取物（4 mg/ml）；对ESBL-KP的MIC（8～16 mg/ml）虽然高于夹竹桃叶（0.224～1.9 mg/ml），但可能是由于本研究采取植物初始重量计算导致浓度偏高[14-15]。此外，RME对部分ESBL-KP菌株的MBC/MIC≤4，表明RME具有一定的杀菌作用[16]。生长曲线和时间-杀菌曲线的结果也进一步证实了RME对ESBL-KP的抑菌和杀菌作用。
本研究使用的D141菌株对多种抗生素耐药，且该菌株同时携带CTX-M、TEM和SHV三种ESBLs，耐药最严重，因此选择其用于研究RME的作用机制，见图2。扫描电镜可准确显示细胞膜形态和超微结构变化，Yuan等[17]采用此技术揭示了玫瑰精油对恶臭假单胞菌细胞膜的破坏。本研究也观察到RME对ESBL-KP菌体细胞膜形态结构的损伤，提示RME破坏细胞膜，进而可导致细胞壁的裂解及细胞内物质丢失，最终导致菌体死亡[18]。
ESBL细菌对β-内酰胺药物的耐药性可以通过添加使用β-内酰胺酶抑制剂得到减轻。植物富含多种生物活性成分，被认为是天然β-内酰胺酶抑制剂的宝库，如印楝叶提取物能够显著降低ESBLs活性[19]。本研究中RME不仅具有抗菌活性，而且对ESBLs也具有抑制作用，提示RME与抗生素联用可能增强药物组合的抑菌或杀菌能力。
本研究在RME中共检出1 550种化合物，然而颜子曦[4]仅检测了粗蛋白、矿质元素、氨基酸等营养物质，杜少波等[13]仅检出63种化合物，本研究提供了更全面的苦水玫瑰成分数据。此外，课题组前期还比较了苦水玫瑰甲醇、乙醇、水提取物的抑菌活性并测定了成分（结果未附），发现甲醇提取物的抗菌活性最好且RME中山奈酚-3-O-芸香糖苷含量远高于乙醇提取物和水提物。该化合物属于黄酮类化合物，具有多种药理学作用，包括抗动脉粥样硬化活性、抗糖尿病活性、抗癌作用、抗病毒活性[20]。Kordbacheh等[21]采用分子对接验证了山奈酚-3-O-芸香糖苷与铜绿假单胞菌群体感应信号受体LasR蛋白的高亲和力，提示其具有潜在的抗群体感应活性。综上，推测RME抑菌活性高于水提物和醇提物，可能与RME中高山奈酚-3-O-芸香糖苷含量相关，值得进一步研究。
综上，本研究首次通过系列实验，筛选并证实了RME对ESBL-KP的广泛抑制活性，其可能通过破坏细胞膜及抑制ESBLs活性发挥抑菌作用，提取物的主要活性成分可能是山奈酚-3-O-芸香糖苷，研究结果对进一步探索苦水玫瑰的抗菌活性积累了宝贵资料，为我国苦水玫瑰抗菌资源的开发利用提供了方向和重要证据。
基金
收起
  • 四川省“十四五”生命健康重大科技项目“重大传染病监测预警与应对(2022ZDZX0017)
文献
收起
参考文献 引证文献
排序方式：
[1]
Ejaz H, Qamar MU, Farhana A, et al. The rising tide of antibiotic resistance: a study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant escherichia coli and klebsiella pneumoniae[J]. J Cli Lab Anal, 2024, 38(10): e25081.
[2]
Mandujano-Hernández A, Martínez-Vázquez AV, Paz-González AD, et al. The global rise of ESBL-Producing escherichia coli in the livestock sector: a Five-Year overview[J]. Animals, 2024, 14(17):2490.
[3]
Husna A, Rahman MM, Badruzzaman ATM, et al.Extended- Spectrum β-Lactamases (ESBL): challenges and opportunities[J]. Biomedicines, 2023, 11(11): 2937.
[4]
颜子曦，李永慧，孟德豪，等.苦水玫瑰中49项营养及活性成分指标分析与评价[J].北京林业大学学报，2023，45（2）：120-128.
Yan ZX，Li YH，Meng DH， et al. Analysis and evaluation of 49 nutritional and active component indexes of Kushui rose (Rosa setate × R. rugosa)[J].Journal of Beijing Forestry University， 2023， 45(2): 120-128.(In Chinese)
[5]
Wu MQ, Xu JY, Li W, et al. Surface morphology and action mechanism of macrophage immunostimulating activity of Rosa Setate x Rosa Rugosa polysaccharide WSRP-1b[J]. Carbohydrate Polymer Technologies and Applications, 2023, 6: 100339.
[6]
马金璞，施生玲，张钰璇，等.超声微波协同萃取法提取兰州苦水玫瑰精油及抑菌活性的研究[J].中国粮油学报，2023，38（12）：151-157.
Ma JP，Shi SL，Zhang YX， et al. Optimized extraction parameters of essential oil from Lanzhou kushui rose by ultrasonic microwave synergistic extraction method using RSM and its antibacterial activities[J]. Journal of the Chinese Cereals and Oils Association， 2023， 38(12): 151-157.(In Chinese)
[7]
Mehta J, Utkarsh K, Fuloria S, et al. Antibacterial potential of bacopa monnieri (L.) wettst. and its bioactive molecules against Uropathogens-An in silico study to identify potential Lead molecule(s) for the development of new drugs to treat urinary tract infections[J]. Molecules, 2022, 27(15): 4971.
[8]
CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically[EB/OL]. [2025-03-15]. https://www.semanticscholar.org/paper/Methods-for-dilution-antimicrobial-susceptibility-%3A-Wikler-Clinical/e418f7892e51569e2357e30ae0cd9a43454b127a.
[9]
褚宽，黄雷，郑阿钦，等.甘胆口服液对副溶血弧菌的抑菌作用及转录组分析[J].微生物学报，2025，65（2）：683-697.
Chu K，Huang L，Zheng AQ， et al. Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrio parahaemolyticus[J]. Acta Microbiologica Sinica， 2025， 65(2): 683-697.(In Chinese)
[10]
Antoniadou M, Rozos G, Vaiou N, et al. The in vitro assessment of antibacterial and antioxidant efficacy in Rosa damascena and hypericum perforatum extracts against pathogenic strains in the interplay of dental caries, oral health, and food microbiota[J].Microorganisms, 2023, 12(1): 60.
[11]
蓝诗华，吴小诗，刘嘉鑫，等.诃子水提物对球形马拉色菌及其生物被膜的抑制作用[J].中草药，2023，54（8）：2480-2487.
Lan SH，Wu XS，Liu JX， et al. Inhibitory effect of water extract of Terminalia chebula on Malassezia globosa and biofilm[J]. Chinese Traditional and Herbal Drugs， 2023， 54(8): 2480-2487.(In Chinese)
[12]
苟玉虹.肉桂醛联合头孢曲松治疗耐药性沙门氏菌感染的药效学研究[D].雅安：四川农业大学，2022.
Gou YH. Pharmacodynamic study of cinnamaldehyde combined with ceftriaxone in the treatment of drug-resistant Salmonella infection[D].Yaan: Sichuan Agriculture University， 2022.(In Chinese)
[13]
杜少波，陈晓文，董志强，等.基于UPLC-Q-TOF-MS的苦水玫瑰醇提物化学成分分析[J].天然产物研究与开发，2022，34（7）：1129-1142.
Du SB，Chen XW，Dong ZQ， et al. Analysis of chemical components in alcohol extract of Rosa sertata×Rosa rugosa based on UPLC-Q-TOF-MS[J]. Natural Product Research and Development， 2022， 34(7): 1129-1142.(In Chinese)
[14]
Cendrowski A, Kraśniewska K, Przybył JL, et al. Antibacterial and antioxidant activity of extracts from rose fruits(Rosa rugosa)[J].Molecules, 2020, 25(6): 1365.
[15]
Hassan MM, Albogami B, Mwabvu T, et al. The antibacterial activity of Rhazya stricta extracts against klebsiella pneumoniae isolated from Some Soil invertebrates at high altitudes[J]. Molecules,2023, 28(8): 3613.
[16]
Golbarg H, Mehdipour moghaddam MJ. Antibacterial potency of medicinal plants including artemisia annua and oxalis corniculata against Multi-Drug resistance E. coil[J]. BioMed Research International, 2021, 2021: 9981915.
[17]
Yuan Y, Liu L, Guo L, et al. Antibacterial mechanism of rose essential oil against Pseudomonas putida isolated from white Hypsizygus marmoreus at cellular and metabolic levels[J]. Industrial Crops and Products, 2023, 196: 116523.
[18]
Islam MS, Aryasomayajula A, Selvaganapathy PR. A review on macroscale and microscale cell lysis methods[J]. Micromachines,2017, 8(3): 83.
[19]
Jassim SA, Hassan MH. Neem leaf extract as a Potential antibiofilm and anti ESBLS agent for K.pneumoniae[J]. Res J Phar Tech, 2023,16(1): 159-162.
[20]
Peng Z, Ma YY, Peng JZ, et al. Kaempferol-3-O-Rutinoside:A natural flavonoid glycosides with multifaceted therapeutic potential[J]. Neurochemical Journal, 2023, 17(2): 247-252.
[21]
Kordbacheh H, Eftekhar F, Ebrahimi SN. Anti-quorum sensing activity of Pistacia atlantica against Pseudomonas aeruginosa PAO1 and identification of its bioactive compounds[J]. Microbial Pathogenesis, 2017, 110: 390-398.
2025年第52卷第7期
PDF下载
67
31
引用本文
BibTeX
文章信息
doi: 10.20043/j.cnki.MPM.202501303
  • 接收时间：2025-01-18
  • 首发时间：2026-03-18
  • 出版时间：2025-04-10
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2025-01-18
基金
四川省“十四五”生命健康重大科技项目“重大传染病监测预警与应对(2022ZDZX0017)
作者信息
    四川大学华西公共卫生学院/华西第四医院，四川 成都 610041

通讯作者:

裴晓方，E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202501303
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持