首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1240722568303398955, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1240722566957027366, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202505059, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1746374400000, receivedDateStr=2025-05-05, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1773740911793, onlineDateStr=2026-03-17, pubDate=1760025600000, pubDateStr=2025-10-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1773740911793, onlineIssueDateStr=2026-03-17, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1773740911793, creator=13701087609, updateTime=1773740911793, updator=13701087609, issue=Issue{id=1240722566957027366, tenantId=1146029695717560320, journalId=1227665162245664772, year='2025', volume='52', issue='19', pageStart='3457', pageEnd='3648', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=0, createTime=1773740911472, creator=13701087609, updateTime=1773740981732, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1240722861736906836, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1240722566957027366, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1240722861736906837, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1240722566957027366, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=3620, endPage=3626, ext={EN=ArticleExt(id=1240722568710246445, articleId=1240722568303398955, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Determination of 15 N-nitrosamines in bacon by QuEChERS purification and gas chromatography-tandem mass spectrometry, columnId=1228016572065837304, journalTitle=Modern Preventive Medicine, columnName=Experimental Technology and Applications, runingTitle=null, highlight=null, articleAbstract=

Objective To establish a method for the rapid detection of 15 N-nitrosamines in bacon by enhanced matrix removal dispersive solid phase extraction combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The samples were extracted with acetonitrile oscillation, frozen for 30 minutes, and then centrifuged at low temperature. The extract was further purified using an enhanced lipid removal purification tube (QuEChERS EMR-lipid) and an EMR-lipid stripping tube. The N-nitrosamines were separated on a polar capillary column, detected in the multiple reaction monitoring (MRM) mode and quantified by the matrix-matched external standard method. Results The linear correlation coefficients of the method were greater than 0.990 in the range of 0.2-40.0 μg/L, with the detection and quantification limits of 0.03-0.30 and 0.1-1.0 μg/kg, respectively. The recoveries were 64.1%-106.1% at three spiked levels (2.0, 5.0, 10.0 μg/kg)，with the relative standard deviations (RSDs) ranging from 3.48% to 14.55%. Conclusion The method does not require activation, equilibration, or elution steps, consumes less solvent, is simple and rapid, and is suitable for the rapid detection of 15 N-nitrosamines in bacon.

, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Quan ZHANG, Lei PENG, Yu-tian WU, Shan BI, Yi-bing ZHOU, Ye LIN, Ting LONG, Qing-yuan CHEN, Hua GUO, Li-ya LIU), CN=ArticleExt(id=1240722569867874378, articleId=1240722568303398955, tenantId=1146029695717560320, journalId=1227665162245664772, language=CN, title=QuEChERS净化-气相色谱-串联质谱法快速测定腊肉中15种N-亚硝胺, columnId=1228016572288135432, journalTitle=现代预防医学, columnName=实验技术及其应用, runingTitle=null, highlight=null, articleAbstract=

目的 建立腊肉中15种N-亚硝胺类化合物的增强型基质去除分散固相萃取结合气相色谱串联质谱（GC-MS/MS）快速测定方法。方法 样品经乙腈振荡提取，冷冻30 min后低温离心，提取液经增强型脂质去除净化管(QuEChERS EMR-Lipid)和EMR-Lipid反萃管进一步净化，目标物经极性毛细管柱分离后以多反应监测模式(MRM)检测和基质匹配外标法定量。结果 在0.2～40.0 μg/L范围内线性相关系数均大于0.990，方法检出限及定量限分别为0.03～0.30 μg/kg和0. 1～1.0 μg/kg。三个水平（2.0、5.0和10.0 μg/kg）的加标回收率范围为64.1%～106.1%，相对标准偏差为3.48%～14.55%。结论 本法无需活化、平衡、洗脱步骤，消耗溶剂较少，操作简单，适用于腊肉中N-亚硝胺类化合物的快速检测。

, correspAuthors=null, authorNote=null, correspAuthorsNote=
刘利亚，E-mail:
, copyrightStatement=本刊刊出的所有文章不代表中华预防医学会和本刊编委会的观点，除非特别声明。, copyrightOwner=中华预防医学会和四川大学华西公共卫生学院, extLink=null, articleAbsUrl=null, sourceXml=DFLx+L8O4djPLlnM1yTDCw==, magXml=ZrN1RgCXIZX05lEnKvsNIA==, pdfUrl=null, pdf=8AIXhCTd4qcMINChIwZ8pQ==, pdfFileSize=1189196, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=2c3X55nrGd/zm1Zs/I0aQw==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=BqubUzorI05CwYtafvXWsg==, mapNumber=null, authorCompany=null, fund=null, authors=

张权（1988—），男，硕士，主管技师，研究方向：食品及环境中污染物分析

, authorsList=张权, 彭蕾, 吴玉田, 毕珊, 周贻兵, 林野, 龙婷, 陈庆园, 郭华, 刘利亚)}, authors=[Author(id=1240933508416327797, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933508533768315, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933508416327797, language=EN, stringName=Quan ZHANG, firstName=Quan, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933508647014528, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933508416327797, language=CN, stringName=张权, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio={"content":"

张权（1988—），男，硕士，主管技师，研究方向：食品及环境中污染物分析

"}, bioImg=null, bioContent=

张权（1988—），男，硕士，主管技师，研究方向：食品及环境中污染物分析

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933508751872135, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933508844146833, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933508751872135, language=EN, stringName=Lei PENG, firstName=Lei, middleName=null, lastName=PENG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933508953198743, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933508751872135, language=CN, stringName=彭蕾, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933509074833567, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933509183885479, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509074833567, language=EN, stringName=Yu-tian WU, firstName=Yu-tian, middleName=null, lastName=WU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933509318103214, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509074833567, language=CN, stringName=吴玉田, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933509410377907, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933509532012731, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509410377907, language=EN, stringName=Shan BI, firstName=Shan, middleName=null, lastName=BI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933509657841861, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509410377907, language=CN, stringName=毕珊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933509750116558, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933509867557079, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509750116558, language=EN, stringName=Yi-bing ZHOU, firstName=Yi-bing, middleName=null, lastName=ZHOU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933509984997596, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933509750116558, language=CN, stringName=周贻兵, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933510098243813, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933510224072942, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510098243813, language=EN, stringName=Ye LIN, firstName=Ye, middleName=null, lastName=LIN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933510345707763, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510098243813, language=CN, stringName=林野, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933510437982459, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933510538645761, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510437982459, language=EN, stringName=Ting LONG, firstName=Ting, middleName=null, lastName=LONG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933510635114756, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510437982459, language=CN, stringName=龙婷, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.铜仁市疾病预防控制中心, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508319858797, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=2., ext=[AuthorCompanyExt(id=1240933508324053102, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508319858797, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.铜仁市疾病预防控制中心)])]), Author(id=1240933510731583755, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=7, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933510844829969, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510731583755, language=EN, stringName=Qing-yuan CHEN, firstName=Qing-yuan, middleName=null, lastName=CHEN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933510953881879, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933510731583755, language=CN, stringName=陈庆园, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933511058739486, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=8, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933511142625570, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933511058739486, language=EN, stringName=Hua GUO, firstName=Hua, middleName=null, lastName=GUO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933511255871781, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933511058739486, language=CN, stringName=郭华, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])]), Author(id=1240933511364923690, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, orderNo=9, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=373604946@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1240933511473975599, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933511364923690, language=EN, stringName=Li-ya LIU, firstName=Li-ya, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1240933511603999029, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, authorId=1240933511364923690, language=CN, stringName=刘利亚, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.贵州省疾病预防控制中心，贵州 贵阳 550004, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)])])], keywords=[Keyword(id=1240933511838880063, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, orderNo=1, keyword=Bacon), Keyword(id=1240933511935349060, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, orderNo=2, keyword=N-nitrosamines), Keyword(id=1240933512052789581, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, orderNo=3, keyword=Gas chromatography-tandem mass spectrometry), Keyword(id=1240933512149258576, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, orderNo=4, keyword=Enhanced matrix removal dispersive solid phase extraction), Keyword(id=1240933512241533267, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, orderNo=1, keyword=腊肉), Keyword(id=1240933512338002266, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, orderNo=2, keyword=N-亚硝胺), Keyword(id=1240933512455442780, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, orderNo=3, keyword=气相色谱-串联质谱法), Keyword(id=1240933512551911779, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, orderNo=4, keyword=增强型基质去除分散固相萃取法)], refs=[Reference(id=1240933514238022058, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=45, issue=21, pageStart=244, pageEnd=249, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=王洪伟, 索化夷, 张玉, journalName=食品与发酵工业, refType=null, unstructuredReference=王洪伟，索化夷，张玉，等.感官评价和GC-MS结合偏最小二乘回归法分析酚类化合物对腊肉烟熏风味的贡献[J].食品与发酵工业，2019，45（21）：244-249., articleTitle=感官评价和GC-MS结合偏最小二乘回归法分析酚类化合物对腊肉烟熏风味的贡献, refAbstract=null), Reference(id=1240933514334491053, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=45, issue=21, pageStart=244, pageEnd=249, url=null, language=null, rfNumber=[1], rfOrder=1, authorNames=Wang HW, Suo HY, Zhang Y, journalName=Food and Fermentation Industries, refType=null, unstructuredReference=Wang HW, Suo HY, Zhang Y, et al. PLSR analysis and sensory evaluation of GC-MS result of phenolic compounds contribution to smoked flavor in Chinese bacon[J]. Food and Fermentation Industries, 2019, 45(21): 244-249.(In Chinese), articleTitle=PLSR analysis and sensory evaluation of GC-MS result of phenolic compounds contribution to smoked flavor in Chinese bacon, refAbstract=null), Reference(id=1240933514426765745, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=38, issue=3, pageStart=30, pageEnd=38, url=null, language=null, rfNumber=[2], rfOrder=2, authorNames=董亚云, 张迎阳, 杨天志, journalName=肉类研究, refType=null, unstructuredReference=董亚云，张迎阳，杨天志，等.基于HS-SPME-GC×GC-TOFMS技术分析传统干腌腊肉加工过程中的挥发性有机物[J].肉类研究，2024，38（3）：30-38., articleTitle=基于HS-SPME-GC×GC-TOFMS技术分析传统干腌腊肉加工过程中的挥发性有机物, refAbstract=null), Reference(id=1240933514493874612, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=38, issue=3, pageStart=30, pageEnd=38, url=null, language=null, rfNumber=[2], rfOrder=3, authorNames=Dong YY, Zhang YY, Yang TZ, journalName=Meat Research, refType=null, unstructuredReference=Dong YY, Zhang YY, Yang TZ, et al. HS-SPME-GC×GC-TOFMS analysis of volatile flavor compounds in traditional Dry-Cured bacon during processing[J]. Meat Research, 2024, 38(3): 30-38.(In Chinese), articleTitle=HS-SPME-GC×GC-TOFMS analysis of volatile flavor compounds in traditional Dry-Cured bacon during processing, refAbstract=null), Reference(id=1240933514581954999, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2020, volume=11, issue=19, pageStart=6829, pageEnd=6834, url=null, language=null, rfNumber=[3], rfOrder=4, authorNames=朱新鹏, 梁如, 张伟艺, journalName=食品安全质量检测学报, refType=null, unstructuredReference=朱新鹏，梁如，张伟艺，等.腊肉的食用安全性及其有害物质控制[J].食品安全质量检测学报，2020，11（19）：6829-6834., articleTitle=腊肉的食用安全性及其有害物质控制, refAbstract=null), Reference(id=1240933514657452474, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2020, volume=11, issue=19, pageStart=6829, pageEnd=6834, url=null, language=null, rfNumber=[3], rfOrder=5, authorNames=Zhu XP, Liang R, Zhang WY, journalName=Journal of Food Safety & Quality, refType=null, unstructuredReference=Zhu XP, Liang R, Zhang WY, et al. Food safety of cured meat and control of its harmful components[J]. Journal of Food Safety & Quality, 2020, 11(19): 6829-6834.(In Chinese), articleTitle=Food safety of cured meat and control of its harmful components, refAbstract=null), Reference(id=1240933514737144255, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=6, authorNames=中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局, journalName=null, refType=null, unstructuredReference=中华人民共和国国家卫生和计划生育委员会， 国家食品药品监督管理总局.GB 5009.26-2023 食品安全国家标准食品中N-亚硝胺类化合物的测定[S].北京：中国标准出版社，2023., articleTitle=null, refAbstract=null), Reference(id=1240933514808447427, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=7, authorNames=The National Health and Family Planning Commission of the People’s Republic of China, the China Food and Drug Administration, journalName=null, refType=null, unstructuredReference=The National Health and Family Planning Commission of the People’s Republic of China, the China Food and Drug Administration. GB 5009.26-2023 National Food Safety Standard Determination of N-nitrosamines in food[S]. Beijing: Standards Press of China, 2023.(In Chinese), articleTitle=null, refAbstract=null), Reference(id=1240933514883944902, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=14, issue=4, pageStart=137, pageEnd=144, url=null, language=null, rfNumber=[5], rfOrder=8, authorNames=李芳芳, 梁秀清, 王艳丽, journalName=食品安全质量检测学报, refType=null, unstructuredReference=李芳芳，梁秀清，王艳丽，等.氢氧化钡处理-分散液液微萃取结合气相色谱-串联质谱法测定肉制品中9种N-亚硝胺[J].食品安全质量检测学报，2023，14（4）：137-144., articleTitle=氢氧化钡处理-分散液液微萃取结合气相色谱-串联质谱法测定肉制品中9种N-亚硝胺, refAbstract=null), Reference(id=1240933514946859465, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=14, issue=4, pageStart=137, pageEnd=144, url=null, language=null, rfNumber=[5], rfOrder=9, authorNames=Li FF, Liang XQ, Wang YL, journalName=Journal of Food Safety and Quality, refType=null, unstructuredReference=Li FF, Liang XQ, Wang YL, et al. Determination of 9 kinds of N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry with Barium hydroxide treatment followed dispersive liquid-liquid microextraction[J]. Journal of Food Safety and Quality, 2023, 14(4):137-144.(In Chinese), articleTitle=Determination of 9 kinds of N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry with Barium hydroxide treatment followed dispersive liquid-liquid microextraction, refAbstract=null), Reference(id=1240933515034939852, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=37, issue=3, pageStart=33, pageEnd=39, url=null, language=null, rfNumber=[6], rfOrder=10, authorNames=王艳丽, 梁秀清, 陈倩倩, journalName=肉类研究, refType=null, unstructuredReference=王艳丽，梁秀清，陈倩倩，等.通过式固相萃取-气相色谱-串联质谱法测定动物源性食品中11种N-亚硝胺类化合物[J].肉类研究，2023，37（3）：33-39., articleTitle=通过式固相萃取-气相色谱-串联质谱法测定动物源性食品中11种N-亚硝胺类化合物, refAbstract=null), Reference(id=1240933515135603151, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=37, issue=3, pageStart=33, pageEnd=39, url=null, language=null, rfNumber=[6], rfOrder=11, authorNames=Wang YL, Liang XQ, Chen QQ, journalName=Meat Research, refType=null, unstructuredReference=Wang YL, Liang XQ, Chen QQ, et al. Determination of eleven N-Nitrosamines in animal derived foods by gas Chromatography-Tandem mass spectrometry after Pass-Through solid phase extraction[J]. Meat Research, 2023, 37(3): 33-39.(In Chinese), articleTitle=Determination of eleven N-Nitrosamines in animal derived foods by gas Chromatography-Tandem mass spectrometry after Pass-Through solid phase extraction, refAbstract=null), Reference(id=1240933515227877843, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=46, issue=1, pageStart=126, pageEnd=131, url=null, language=null, rfNumber=[7], rfOrder=12, authorNames=何健, 赵舰, 唐晓琴, journalName=现代预防医学, refType=null, unstructuredReference=何健，赵舰，唐晓琴，等.加速溶剂萃取/凝胶渗透色谱净化-气质联用法测定烟熏腊肉中24种多环芳烃[J].现代预防医学，2019，46（1）：126-131., articleTitle=加速溶剂萃取/凝胶渗透色谱净化-气质联用法测定烟熏腊肉中24种多环芳烃, refAbstract=null), Reference(id=1240933515294986712, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=46, issue=1, pageStart=126, pageEnd=131, url=null, language=null, rfNumber=[7], rfOrder=13, authorNames=He J, Zhao J, Tang XQ, journalName=Modern Preventive Medicine, refType=null, unstructuredReference=He J, Zhao J, Tang XQ, et al. Determination of 24 Polycyclic Aromatic Hydrocarbons in Smoked meat by ASE extraction-GPC purification combined with gas chromatography-mass spectrometry[J]. Modern Preventive Medicine, 2019, 46(1): 126-131.(In Chinese), articleTitle=Determination of 24 Polycyclic Aromatic Hydrocarbons in Smoked meat by ASE extraction-GPC purification combined with gas chromatography-mass spectrometry, refAbstract=null), Reference(id=1240933515366289881, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=39, issue=1, pageStart=96, pageEnd=103, url=null, language=null, rfNumber=[8], rfOrder=14, authorNames=孔祥一, 庄丽丽, 方恩华, journalName=色谱, refType=null, unstructuredReference=孔祥一，庄丽丽，方恩华，等.QuEChERS-同位素稀释-气相色谱-串联质谱法测定动物源性食品中9种N-亚硝胺类化合物[J].色谱，2021，39（1）：96-103., articleTitle=QuEChERS-同位素稀释-气相色谱-串联质谱法测定动物源性食品中9种N-亚硝胺类化合物, refAbstract=null), Reference(id=1240933515462758876, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=39, issue=1, pageStart=96, pageEnd=103, url=null, language=null, rfNumber=[8], rfOrder=15, authorNames=Kong XY, Zhuang LL, Fang EH, journalName=Chinese Journal of Chromatography, refType=null, unstructuredReference=Kong XY, Zhuang LL, Fang EH, et al. Determination of nine N-nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2021, 39(1): 96-103.(In Chinese), articleTitle=Determination of nine N-nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry, refAbstract=null), Reference(id=1240933515542450654, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=37, issue=2, pageStart=207, pageEnd=215, url=null, language=null, rfNumber=[9], rfOrder=16, authorNames=朱萌萌, 叶群, 周婷婷, journalName=色谱, refType=null, unstructuredReference=朱萌萌，叶群，周婷婷，等. 气相色谱-串联质谱法测定肉制品中10种挥发性N-亚硝胺类化合物[J].色谱，2019，37（2）：207-215., articleTitle=气相色谱-串联质谱法测定肉制品中10种挥发性N-亚硝胺类化合物, refAbstract=null), Reference(id=1240933515609559522, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2019, volume=37, issue=2, pageStart=207, pageEnd=215, url=null, language=null, rfNumber=[9], rfOrder=17, authorNames=Zhu MM, Ye Q, Zhou TT, journalName=Chinese Journal of Chromatography, refType=null, unstructuredReference=Zhu MM, Ye Q, Zhou TT, et al. Determination of 10 volatile N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(2): 207-215.(In Chinese), articleTitle=Determination of 10 volatile N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry, refAbstract=null), Reference(id=1240933515689251302, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=42, issue=5, pageStart=465, pageEnd=473, url=null, language=null, rfNumber=[10], rfOrder=18, authorNames=夏寒, 仝凯旋, 朱浙辉, journalName=色谱, refType=null, unstructuredReference=夏寒，仝凯旋，朱浙辉，等.一步式QuEChERS-气相色谱-三重四极杆质谱法快速测定风干牦牛肉中15种N-亚硝胺[J].色谱，2024，42（5）：465-473., articleTitle=一步式QuEChERS-气相色谱-三重四极杆质谱法快速测定风干牦牛肉中15种N-亚硝胺, refAbstract=null), Reference(id=1240933515768943081, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=42, issue=5, pageStart=465, pageEnd=473, url=null, language=null, rfNumber=[10], rfOrder=19, authorNames=Xia H, Tong KX, Zhu ZH, journalName=Chinese Journal of Chromatography, refType=null, unstructuredReference=Xia H, Tong KX, Zhu ZH, et al. Rapid determination of 15 N-nitrosamines in air-dried yak meat using one-step QuEChERS-gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2024, 42(5): 465-473.(In Chinese), articleTitle=Rapid determination of 15 N-nitrosamines in air-dried yak meat using one-step QuEChERS-gas chromatography-triple quadrupole mass spectrometry, refAbstract=null), Reference(id=1240933515848634861, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=44, issue=7, pageStart=291, pageEnd=294, url=null, language=null, rfNumber=[11], rfOrder=20, authorNames=沈昌莹, 苏骏敏, 莫淑梅, journalName=食品工业, refType=null, unstructuredReference=沈昌莹，苏骏敏，莫淑梅，等.QuEChERS结合GC-MS/MS测定腌腊肉制品中N-二甲基亚硝胺[J].食品工业，2023，44（7）：291-294., articleTitle=QuEChERS结合GC-MS/MS测定腌腊肉制品中N-二甲基亚硝胺, refAbstract=null), Reference(id=1240933515987046896, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=44, issue=7, pageStart=291, pageEnd=294, url=null, language=null, rfNumber=[11], rfOrder=21, authorNames=Shen CY, Su JM, Mo SM, journalName=The Food Industry, refType=null, unstructuredReference=Shen CY, Su JM, Mo SM, et al. Determination of N-Dimethylnitrosamine in preserved meat products by QuEChERS combined with GC-MS/MS[J]. The Food Industry, 2023, 44(7): 291-294.(In Chinese), articleTitle=Determination of N-Dimethylnitrosamine in preserved meat products by QuEChERS combined with GC-MS/MS, refAbstract=null), Reference(id=1240933516083515891, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=48, issue=9, pageStart=1667, pageEnd=1671, url=null, language=null, rfNumber=[12], rfOrder=22, authorNames=袁娅, 周鸿, 宋迎春, journalName=现代预防医学, refType=null, unstructuredReference=袁娅，周鸿，宋迎春，等.同位素稀释-气相色谱串联质谱法测定啤酒中4种亚硝胺类化合物[J].现代预防医学，2021，48（9）：1667-1671., articleTitle=同位素稀释-气相色谱串联质谱法测定啤酒中4种亚硝胺类化合物, refAbstract=null), Reference(id=1240933516179984885, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=48, issue=9, pageStart=1667, pageEnd=1671, url=null, language=null, rfNumber=[12], rfOrder=23, authorNames=Yuan Y, Zhou H, Song YC, journalName=Modern Preventive Medicine, refType=null, unstructuredReference=Yuan Y, Zhou H, Song YC, et al. Determination of four N - nitrosamines in beer by isotope dilutiongas Chromatography-tandem mass spectrometry[J].Modern Preventive Medicine, 2021, 48(9): 1667-1671.(In Chinese), articleTitle=Determination of four N - nitrosamines in beer by isotope dilutiongas Chromatography-tandem mass spectrometry, refAbstract=null), Reference(id=1240933516255482359, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2020, volume=41, issue=20, pageStart=198, pageEnd=203, url=null, language=null, rfNumber=[13], rfOrder=24, authorNames=马兴, 肖亚兵, 陈颖, journalName=食品研究与开发, refType=null, unstructuredReference=马兴，肖亚兵，陈颖，等.快速水蒸气蒸馏-气相色谱热能分析仪法测定肉制品和水产制品中13种N-亚硝胺[J].食品研究与开发，2020，41（20）：198-203., articleTitle=快速水蒸气蒸馏-气相色谱热能分析仪法测定肉制品和水产制品中13种N-亚硝胺, refAbstract=null), Reference(id=1240933516335174137, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2020, volume=41, issue=20, pageStart=198, pageEnd=203, url=null, language=null, rfNumber=[13], rfOrder=25, authorNames=Ma X, Xiao YB, Chen Y, journalName=Food Research and Development, refType=null, unstructuredReference=Ma X, Xiao YB, Chen Y, et al. Determination of 13 N-Nitrosamines in meat and aquatic products by gas Chromatography-Thermal energy analyzer with rapid steam distillation[J]. Food Research and Development,2020, 41(20): 198-203.(In Chinese), articleTitle=Determination of 13 N-Nitrosamines in meat and aquatic products by gas Chromatography-Thermal energy analyzer with rapid steam distillation, refAbstract=null), Reference(id=1240933516406477307, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=42, issue=20, pageStart=266, pageEnd=273, url=null, language=null, rfNumber=[14], rfOrder=26, authorNames=戴裕杰, 关荣发, 黄海智, journalName=食品科学, refType=null, unstructuredReference=戴裕杰，关荣发，黄海智，等.改进的QuEChERS-气相色谱-质谱法测定中式腊肉中8种挥发性N-亚硝胺[J].食品科学，2021，42（20）：266-273., articleTitle=改进的QuEChERS-气相色谱-质谱法测定中式腊肉中8种挥发性N-亚硝胺, refAbstract=null), Reference(id=1240933516498751998, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=42, issue=20, pageStart=266, pageEnd=273, url=null, language=null, rfNumber=[14], rfOrder=27, authorNames=Dai YJ, Guan RF, Huang HZ, journalName=Food Science, refType=null, unstructuredReference=Dai YJ, Guan RF, Huang HZ, et al. Determination of eight volatile N-Nitrosamines in Chinese bacon by modified QuEChERS and gas Chromatography-Mass spectrometry[J]. Food Science, 2021, 42(20): 266-273.(In Chinese), articleTitle=Determination of eight volatile N-Nitrosamines in Chinese bacon by modified QuEChERS and gas Chromatography-Mass spectrometry, refAbstract=null), Reference(id=1240933516586832382, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=53, issue=11, pageStart=1334, pageEnd=1339, url=null, language=null, rfNumber=[15], rfOrder=28, authorNames=张静, 赖俊敏, 李思源, journalName=日用化学工业, refType=null, unstructuredReference=张静，赖俊敏，李思源，等.气相色谱-三重四极杆质谱法测定染发剂中14种N-亚硝胺类化合物[J].日用化学工业，2023，53（11）：1334-1339., articleTitle=气相色谱-三重四极杆质谱法测定染发剂中14种N-亚硝胺类化合物, refAbstract=null), Reference(id=1240933516649746944, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2023, volume=53, issue=11, pageStart=1334, pageEnd=1339, url=null, language=null, rfNumber=[15], rfOrder=29, authorNames=Zhang J, Lai JM, Li SY, journalName=China Surfactant Detergent & Cosmetics, refType=null, unstructuredReference=Zhang J, Lai JM, Li SY, et al. Determination of 14 N-nitrosamines in hair dyes using gas chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1334-1339.(In Chinese), articleTitle=Determination of 14 N-nitrosamines in hair dyes using gas chromatography-tandem mass spectrometry, refAbstract=null), Reference(id=1240933516712661506, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=47, issue=16, pageStart=247, pageEnd=252, url=null, language=null, rfNumber=[16], rfOrder=30, authorNames=李林竹, 马凯, 杨昌彪, journalName=食品与发酵工业, refType=null, unstructuredReference=李林竹，马凯，杨昌彪，等.QuEChERS-超高效液相色谱-串联质谱法检测腌腊肉中四种常用香料[J].食品与发酵工业，2021，47（16）：247-252., articleTitle=QuEChERS-超高效液相色谱-串联质谱法检测腌腊肉中四种常用香料, refAbstract=null), Reference(id=1240933516783964676, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2021, volume=47, issue=16, pageStart=247, pageEnd=252, url=null, language=null, rfNumber=[16], rfOrder=31, authorNames=Li LZ, Ma K, Yang CB, journalName=Food and Fermentation Industries, refType=null, unstructuredReference=Li LZ, Ma K, Yang CB, et al. Determination of four spices in cured meat samples by QuEChERS coupled with ultra-performance liquid chomatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021, 47(16): 247-252.(In Chinese), articleTitle=Determination of four spices in cured meat samples by QuEChERS coupled with ultra-performance liquid chomatography-tandem mass spectrometry, refAbstract=null), Reference(id=1240933516863656454, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=3, pageStart=523, pageEnd=528, url=null, language=null, rfNumber=[17], rfOrder=32, authorNames=夏仕青, 吴桃丽, 薛云, journalName=现代预防医学, refType=null, unstructuredReference=夏仕青，吴桃丽，薛云，等.QuEChERs结合气相色谱-串联质谱法测定不同果蔬基质中33种有机磷类农药残留量[J].现代预防医学，2024，51（3）：523-528., articleTitle=QuEChERs结合气相色谱-串联质谱法测定不同果蔬基质中33种有机磷类农药残留量, refAbstract=null), Reference(id=1240933516947542536, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=3, pageStart=523, pageEnd=528, url=null, language=null, rfNumber=[17], rfOrder=33, authorNames=Xia SQ, Wu TL, Xue Y, journalName=Modern Preventive Medicine, refType=null, unstructuredReference=Xia SQ, Wu TL, Xue Y, et al. Determination of 33 organophosphorus pesticide residues in different fruit and vegetable substrates by QuEChERs combined with gas chromatogra-phy-tandem mass spectrometry[J].Modern Preventive Medicine, 2024, 51(3): 523-528.(In Chinese), articleTitle=Determination of 33 organophosphorus pesticide residues in different fruit and vegetable substrates by QuEChERs combined with gas chromatogra-phy-tandem mass spectrometry, refAbstract=null), Reference(id=1240933517006262794, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=4, pageStart=722, pageEnd=727, url=null, language=null, rfNumber=[18], rfOrder=34, authorNames=张艳, 曲良娇, 凌莉, journalName=现代预防医学, refType=null, unstructuredReference=张艳，曲良娇，凌莉，等.UPLC-MS/MS法同时测定蔬菜中11种有机磷和10种新烟碱类杀虫剂[J].现代预防医学，2024，51（4）：722-727., articleTitle=UPLC-MS/MS法同时测定蔬菜中11种有机磷和10种新烟碱类杀虫剂, refAbstract=null), Reference(id=1240933517085954572, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=4, pageStart=722, pageEnd=727, url=null, language=null, rfNumber=[18], rfOrder=35, authorNames=Zhang Y, Qu LJ, Ling L, journalName=Modern Preventive Medicine, refType=null, unstructuredReference=Zhang Y, Qu LJ, Ling L, et al. Simultaneous determination of eleven organophosphorus and ten neonicotinoid pesticide in vegetables by UPLC-MS/MS[J]. Modern Preventive Medicine, 2024, 51(4): 722-727.(In Chinese), articleTitle=Simultaneous determination of eleven organophosphorus and ten neonicotinoid pesticide in vegetables by UPLC-MS/MS, refAbstract=null), Reference(id=1240933517153063438, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=4, pageStart=713, pageEnd=721, url=null, language=null, rfNumber=[19], rfOrder=36, authorNames=李四生, 张维, 章剑, journalName=现代预防医学, refType=null, unstructuredReference=李四生，张维，章剑，等.QuEChERS-超高效液相色谱-三重四级杆质谱法同时测定尿液和血液中22种植物毒素[J].现代预防医学，2024，51（4）：713-721., articleTitle=QuEChERS-超高效液相色谱-三重四级杆质谱法同时测定尿液和血液中22种植物毒素, refAbstract=null), Reference(id=1240933517245338128, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=51, issue=4, pageStart=713, pageEnd=721, url=null, language=null, rfNumber=[19], rfOrder=37, authorNames=Li SS, Zhang W, Zhang J, journalName=Modern Preventive Medicine, refType=null, unstructuredReference=Li SS, Zhang W, Zhang J, et al. Simultaneous determination of 22 phytotoxins in urine/blood by QuEChERS-Ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. Modern Preventive Medicine, 2024, 51(4): 713-721.(In Chinese), articleTitle=Simultaneous determination of 22 phytotoxins in urine/blood by QuEChERS-Ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry, refAbstract=null), Reference(id=1240933517337612818, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=50, issue=5, pageStart=226, pageEnd=233, url=null, language=null, rfNumber=[20], rfOrder=38, authorNames=范小宁, 师希雄, 包晓明, journalName=食品与发酵工业, refType=null, unstructuredReference=范小宁，师希雄，包晓明，等.陇西腊肉加工过程中亚硝胺与理化指标的相关性分析[J].食品与发酵工业，2024，50（5）：226-233., articleTitle=陇西腊肉加工过程中亚硝胺与理化指标的相关性分析, refAbstract=null), Reference(id=1240933517408915988, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, doi=null, pmid=null, pmcid=null, year=2024, volume=50, issue=5, pageStart=226, pageEnd=233, url=null, language=null, rfNumber=[20], rfOrder=39, authorNames=Fan XN, Shi XX, Bao XM, journalName=Food and Fermentation Industries, refType=null, unstructuredReference=Fan XN, Shi XX, Bao XM, et al. Correlation analysis of nitrosamines and physical and chemical indexes during Longxi bacon processing[J]. Food and Fermentation Industries, 2024, 50(5): 226-233.(In Chinese), articleTitle=Correlation analysis of nitrosamines and physical and chemical indexes during Longxi bacon processing, refAbstract=null)], funds=[Fund(id=1240933513810203032, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, awardId=gzwkj2024-224, language=CN, fundingSource=贵州省卫生健康委科学技术基金项目(gzwkj2024-224), fundOrder=null, country=null), Fund(id=1240933513919254942, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, awardId=bsgzs2025-01, language=CN, fundingSource=贵州疾控博士工作室(bsgzs2025-01), fundOrder=null, country=null), Fund(id=1240933514049278369, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, awardId=null, language=CN, fundingSource=贵州省卫生健康委省级重点建设学科项目, fundOrder=null, country=null)], companyList=[AuthorCompany(id=1240933508244361316, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=1., ext=[AuthorCompanyExt(id=1240933508252749925, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China), AuthorCompanyExt(id=1240933508261138534, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508244361316, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.贵州省疾病预防控制中心，贵州 贵阳 550004)]), AuthorCompany(id=1240933508319858797, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, xref=2., ext=[AuthorCompanyExt(id=1240933508324053102, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, companyId=1240933508319858797, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.铜仁市疾病预防控制中心)])], figs=[ArticleFig(id=1240933512887456110, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, label=Figure 1, caption=The recovery comparisom of different extraction solvents, figureFileSmall=YxVKIWy3UpIgzNxRZnqllQ==, figureFileBig=fLL6jFiN9wglMbnZT9gLJg==, tableContent=null), ArticleFig(id=1240933512971342196, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, label=图1, caption=不同提取剂的回收率比较, figureFileSmall=YxVKIWy3UpIgzNxRZnqllQ==, figureFileBig=fLL6jFiN9wglMbnZT9gLJg==, tableContent=null), ArticleFig(id=1240933513067811192, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, label=Figure 2, caption=The full scan spectrogram of the sample solutions by different purification methods, figureFileSmall=oN7S/a5oOWQzmIDx1Dw2Rg==, figureFileBig=9AL8Wvul2tCmpFnFcaY3UA==, tableContent=null), ArticleFig(id=1240933513151697275, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, label=图2, caption=不同净化方式的样品液全扫谱图

注：图A为Dima QuEChERS；图B为Agela QuEChERS；图C为EMR-Lipid dSPE；图D为LPAS-SPE；图E为HLB-SPE；图F为Captiva EMR-Lipid HF-SPE；图G为CNW QuEChERS。

, figureFileSmall=oN7S/a5oOWQzmIDx1Dw2Rg==, figureFileBig=9AL8Wvul2tCmpFnFcaY3UA==, tableContent=null), ArticleFig(id=1240933513235583360, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, label=Figure 3, caption=Total ion chromatogram of a spiked bacon sample, figureFileSmall=1Ts61Qt+srLdlKT0gD/dWA==, figureFileBig=d/iCla1FbozmIiwszm3Igw==, tableContent=null), ArticleFig(id=1240933513319469444, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, label=图3, caption=腊肉加标样品的总离子流色谱图, figureFileSmall=1Ts61Qt+srLdlKT0gD/dWA==, figureFileBig=d/iCla1FbozmIiwszm3Igw==, tableContent=null), ArticleFig(id=1240933513390772615, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, label=Table 1, caption=

The mass spectrometric parameters of the method

, figureFileSmall=null, figureFileBig=null, tableContent=
序号化合物化学式CAS保留时间（min）离子对(m/z)碰撞能量（eV）
1N-亚硝基二甲胺(NDMA)C2H6N2O62-75-95.8174.0>44.0*,74.0>42.13, 19
2N-亚硝基甲乙胺(NMEA)C3H8N2O10595-95-66.4088.0>71.1*,88.0>42.02, 17
3N-亚硝胺二乙胺(NDEA)C4H10N2O55-18-56.75102.0>85.0*,102.0>56.02, 15
4N-亚硝胺吡咯啶(NPYR)C4H8N2O930-55-211.69100.0>55.1*,100.0>43.15, 9
5N-亚硝胺甲基苯胺(NMPhA)C7H5N2O614-00-610.94107.0>106.0*,107.0>77.010, 15
6N-亚硝胺吗啉(NMorPh)C4H8N2O259-89-212.66116.0>56.0*,116.2>86.111, 5
7N-亚硝胺二丙胺(NDPA)C6H14N2O621-64-78.43130.2>43.0*,130.2>113.19, 7
8N-亚硝胺哌啶(NPIP)C5H10N2O100-75-411.16114.0>84.0*,114.0>41.05, 13
9N-亚硝胺乙基苯胺(NEPhA)C8H10N2O612-64-610.99121.0>106.0*,121.0>77.05, 20
1
0		N-亚硝基二正丁胺(NDBA)C8H18N2O924-16-310.78116.0>99.0*,116.0>74.12, 8
11N-亚硝胺二苯胺(NDPhA)C12H10N2O86-30-618.91169.0>168.1*,169.0>167.117, 30
12N-亚硝胺二苄胺(NDBzA)C14H14N2O5336-53-821.95226.0>91.0*,91.0>65.05, 5
13N-亚硝胺乙基异丙基胺(NEIPA)C5H12N2O16339-04-17.17116.0>99.0*,116.0>44.05, 15
14N-亚硝胺二异丙胺(NDiPA)C6H14N2O601-77-47.49130.0>88.0*,130.0>42.05, 10
15N-亚硝胺二异丁胺(NDIBA)C8H18N2O997-95-58.6984.0>57.0*,115.0>84.05, 3
), ArticleFig(id=1240933513470464393, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, label=表1, caption=

方法的质谱参数

, figureFileSmall=null, figureFileBig=null, tableContent=
序号化合物化学式CAS保留时间（min）离子对(m/z)碰撞能量（eV）
1N-亚硝基二甲胺(NDMA)C2H6N2O62-75-95.8174.0>44.0*,74.0>42.13, 19
2N-亚硝基甲乙胺(NMEA)C3H8N2O10595-95-66.4088.0>71.1*,88.0>42.02, 17
3N-亚硝胺二乙胺(NDEA)C4H10N2O55-18-56.75102.0>85.0*,102.0>56.02, 15
4N-亚硝胺吡咯啶(NPYR)C4H8N2O930-55-211.69100.0>55.1*,100.0>43.15, 9
5N-亚硝胺甲基苯胺(NMPhA)C7H5N2O614-00-610.94107.0>106.0*,107.0>77.010, 15
6N-亚硝胺吗啉(NMorPh)C4H8N2O259-89-212.66116.0>56.0*,116.2>86.111, 5
7N-亚硝胺二丙胺(NDPA)C6H14N2O621-64-78.43130.2>43.0*,130.2>113.19, 7
8N-亚硝胺哌啶(NPIP)C5H10N2O100-75-411.16114.0>84.0*,114.0>41.05, 13
9N-亚硝胺乙基苯胺(NEPhA)C8H10N2O612-64-610.99121.0>106.0*,121.0>77.05, 20
1
0		N-亚硝基二正丁胺(NDBA)C8H18N2O924-16-310.78116.0>99.0*,116.0>74.12, 8
11N-亚硝胺二苯胺(NDPhA)C12H10N2O86-30-618.91169.0>168.1*,169.0>167.117, 30
12N-亚硝胺二苄胺(NDBzA)C14H14N2O5336-53-821.95226.0>91.0*,91.0>65.05, 5
13N-亚硝胺乙基异丙基胺(NEIPA)C5H12N2O16339-04-17.17116.0>99.0*,116.0>44.05, 15
14N-亚硝胺二异丙胺(NDiPA)C6H14N2O601-77-47.49130.0>88.0*,130.0>42.05, 10
15N-亚硝胺二异丁胺(NDIBA)C8H18N2O997-95-58.6984.0>57.0*,115.0>84.05, 3
), ArticleFig(id=1240933513541767566, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=EN, label=Table 2, caption=

The performance parameters of the method（n=6）

, figureFileSmall=null, figureFileBig=null, tableContent=
被测化合物线性范围
(μg/L)		回归方程相关系数
（r2）		检出限
(μg/kg)		定量限
(μg/kg)		2.00 μg/kg 5.00 μg/kg 10.0 μg/kg
回收率
（%）		RSD
（%）		回收率
（%）		RSD
（%）		回收率
（%）		RSD
（%）
NDMA0.2～40.0Y=3.311×103X+7.830×1050.996 40.150.587.29.3694.413.8687.910.71
NMEA0.2～40.0Y=3.027×103X-4.002×1030.998 80.150.593.47.5595.26.0283.38.92
NDEA0.2～40.0Y=2.313×103X-1.195×1030.999 20.150.585.39.4884.511.3776.710.25
NPYR0.2～40.0Y=1.889×103X-2.395×1030.998 40.150.584.514.3388.114.3881.53.48
NMPhA0.2～40.0Y=1.193×104X-1.990×1040.996 80.030.199.17.7985.43.5786.314.28
NMorPh0.2～40.0Y=9.347×102X-6.099×1030.999 20.150.584.310.3094.64.6981.59.39
NDPA0.2～40.0Y=5.091×102X-5.373×1020.999 40.301.088.813.5693.112.0583.57.88
NPIP0.2～40.0Y=1.876×103X-4.781×1030.997 70.150.596.112.5895.711.3986.110.54
NEPhA0.2～40.0Y=1.140×104X+4.497×1030.999 00.030.177.18.6787.98.5483.84.77
NDBA0.2～40.0Y=2.090×103X-8.528×1020.998 60.150.585.610.3693.09.3682.810.81
NDPhA0.2～40.0Y=1.088×105X+4.258×1050.999 60.030.1106.15.36101.17.5886.88.36
NDBzA0.2～40.0Y=1.038×103X-9.962×1020.999 00.150.591.57.4880.98.9580.712.38
NEIPA0.2～40.0Y=2.772×103X-1.152×1030.998 80.150.564.112.3973.76.5281.814.55
NDiPA0.2～40.0Y=1.213×103X-6.851×1020.997 80.150.578.410.3699.07.3687.27.96
NDIBA0.2～40.0Y=5.972×102X-2.420×1010.996 90.100.382.314.2183.811.375.04.56
), ArticleFig(id=1240933513646625168, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240722568303398955, language=CN, label=表2, caption=

方法的性能参数（n=6）

, figureFileSmall=null, figureFileBig=null, tableContent=
被测化合物线性范围
(μg/L)		回归方程相关系数
（r2）		检出限
(μg/kg)		定量限
(μg/kg)		2.00 μg/kg 5.00 μg/kg 10.0 μg/kg
回收率
（%）		RSD
（%）		回收率
（%）		RSD
（%）		回收率
（%）		RSD
（%）
NDMA0.2～40.0Y=3.311×103X+7.830×1050.996 40.150.587.29.3694.413.8687.910.71
NMEA0.2～40.0Y=3.027×103X-4.002×1030.998 80.150.593.47.5595.26.0283.38.92
NDEA0.2～40.0Y=2.313×103X-1.195×1030.999 20.150.585.39.4884.511.3776.710.25
NPYR0.2～40.0Y=1.889×103X-2.395×1030.998 40.150.584.514.3388.114.3881.53.48
NMPhA0.2～40.0Y=1.193×104X-1.990×1040.996 80.030.199.17.7985.43.5786.314.28
NMorPh0.2～40.0Y=9.347×102X-6.099×1030.999 20.150.584.310.3094.64.6981.59.39
NDPA0.2～40.0Y=5.091×102X-5.373×1020.999 40.301.088.813.5693.112.0583.57.88
NPIP0.2～40.0Y=1.876×103X-4.781×1030.997 70.150.596.112.5895.711.3986.110.54
NEPhA0.2～40.0Y=1.140×104X+4.497×1030.999 00.030.177.18.6787.98.5483.84.77
NDBA0.2～40.0Y=2.090×103X-8.528×1020.998 60.150.585.610.3693.09.3682.810.81
NDPhA0.2～40.0Y=1.088×105X+4.258×1050.999 60.030.1106.15.36101.17.5886.88.36
NDBzA0.2～40.0Y=1.038×103X-9.962×1020.999 00.150.591.57.4880.98.9580.712.38
NEIPA0.2～40.0Y=2.772×103X-1.152×1030.998 80.150.564.112.3973.76.5281.814.55
NDiPA0.2～40.0Y=1.213×103X-6.851×1020.997 80.150.578.410.3699.07.3687.27.96
NDIBA0.2～40.0Y=5.972×102X-2.420×1010.996 90.100.382.314.2183.811.375.04.56
)], attaches=null, journal=Journal(id=1227664546253402114, delFlag=0, nameCn=现代预防医学, nameEn=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, issn=1003-8507, eissn=null, cn=51-1365/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=IeiuPXEZi6AA+k0VfvoiOQ==, journalPrice=null, startedYear=null, abbrevIsoEn=Modern Preventive Medicine, journalRemark=null, publicationField=null, createdTime=1770627636734, updatedTime=1770628902248, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=M, firstLetterEn=M, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=IeiuPXEZi6AA+k0VfvoiOQ==, picEn=/9iTl8/ndms4tBz1fL28Pg==, jcr=null, cjcr=null, exts=[JournalExt(id=1227669854342280188, language=CN, name=现代预防医学, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902278, updatedTime=1770628902278, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1227669854396806141, language=EN, name=Modern Preventive Medicine, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1770628902291, updatedTime=1770628902291, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://xdyfyxzz.paperopen.com/#/regist, submissionEditorUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionReviewUrl=http://xdyfyxzz.paperopen.com/#/Login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1227665162245664772, websiteList=[Website(id=1227687234141352800, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/CN, language=CN, createTime=1770633045945, createBy=18614031015, updateTime=1770633090526, updateBy=18614031015, name=现代预防医学-中文, tplId=1146099689490845704, title=现代预防医学, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687735088051072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=articleTextType, value=kx, createTime=1770633165380, updateTime=1770633165380, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735071273853, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=banner, value=null, createTime=1770633165376, updateTime=1770633165376, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735113216899, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=grayFlag, value=0, createTime=1770633165386, updateTime=1770633165386, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735062885244, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633165374, updateTime=1770633165374, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735125799813, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=minRunFlag, value=0, createTime=1770633165389, updateTime=1770633165389, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735083856767, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/file/pic, createTime=1770633165379, updateTime=1770633165379, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735121605508, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=silenceFlag, value=0, createTime=1770633165388, updateTime=1770633165388, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735079662462, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1770633165378, updateTime=1770633165378, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735096439681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeColor, value=null, createTime=1770633165382, updateTime=1770633165382, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687735104828290, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234141352800, code=themeStyle, value=null, createTime=1770633165384, updateTime=1770633165384, creator=18614031015, updator=18614031015)]), Website(id=1227687234338485094, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1227665162245664772, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyfyx/EN, language=EN, createTime=1770633045992, createBy=18614031015, updateTime=1770633115374, updateBy=18614031015, name=现代预防医学-英文, tplId=1146101810881728533, title=Modern Preventive Medicine, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1227687709129507332, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=articleTextType, value=kx, createTime=1770633159191, updateTime=1770633159191, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709108535809, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=banner, value=null, createTime=1770633159186, updateTime=1770633159186, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709167256071, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=grayFlag, value=0, createTime=1770633159200, updateTime=1770633159200, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709095952896, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic?fileId=/XB5plC0xuykmQnycvtyrw==, createTime=1770633159183, updateTime=1770633159183, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709179838985, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=minRunFlag, value=0, createTime=1770633159203, updateTime=1770633159203, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709121118723, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/file/pic, createTime=1770633159189, updateTime=1770633159189, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709171450376, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=silenceFlag, value=0, createTime=1770633159201, updateTime=1770633159201, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709112730114, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1770633159187, updateTime=1770633159187, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709133701637, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeColor, value=null, createTime=1770633159192, updateTime=1770633159192, creator=18614031015, updator=18614031015), WebsiteProps(id=1227687709154673158, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1227687234338485094, code=themeStyle, value=null, createTime=1770633159197, updateTime=1770633159197, creator=18614031015, updator=18614031015)])], journalTitle=现代预防医学, weixinUrl=null, journalUrl=http://xdyfyxzz.paperopen.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Modern Preventive Medicine, journalPhotoCn=IeiuPXEZi6AA+k0VfvoiOQ==, journalPhotoEn=/9iTl8/ndms4tBz1fL28Pg==, journalFirstLetter=M, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202505059, detailUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/10.20043/j.cnki.MPM.202505059, pdfUrlCn=https://castjournals.cast.org.cn/joweb/xdyfyx/CN/PDF/10.20043/j.cnki.MPM.202505059, pdfUrlEn=https://castjournals.cast.org.cn/joweb/xdyfyx/EN/PDF/10.20043/j.cnki.MPM.202505059, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
QuEChERS净化-气相色谱-串联质谱法快速测定腊肉中15种N-亚硝胺
收藏切换
PDF下载
张权 1 , 彭蕾 1 , 吴玉田 1 , 毕珊 1 , 周贻兵 1 , 林野 1 , 龙婷 2 , 陈庆园 1 , 郭华 1 , 刘利亚 1
现代预防医学 | 实验技术及其应用 2025,52(19): 3620-3626
收起
收藏切换
现代预防医学 | 实验技术及其应用 2025, 52(19): 3620-3626
QuEChERS净化-气相色谱-串联质谱法快速测定腊肉中15种N-亚硝胺
全屏
张权1, 彭蕾1, 吴玉田1, 毕珊1, 周贻兵1, 林野1, 龙婷2, 陈庆园1, 郭华1, 刘利亚1
作者信息
  • 1.贵州省疾病预防控制中心，贵州 贵阳 550004
  • 2.铜仁市疾病预防控制中心

    • 张权（1988—），男，硕士，主管技师，研究方向：食品及环境中污染物分析

通讯作者:

刘利亚，E-mail:
Determination of 15 N-nitrosamines in bacon by QuEChERS purification and gas chromatography-tandem mass spectrometry
Quan ZHANG1, Lei PENG1, Yu-tian WU1, Shan BI1, Yi-bing ZHOU1, Ye LIN1, Ting LONG2, Qing-yuan CHEN1, Hua GUO1, Li-ya LIU1
Affiliations
  • Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China
出版时间: 2025-10-10 doi: 10.20043/j.cnki.MPM.202505059
文章导航
收藏切换
摘要
收起

目的 建立腊肉中15种N-亚硝胺类化合物的增强型基质去除分散固相萃取结合气相色谱串联质谱（GC-MS/MS）快速测定方法。方法 样品经乙腈振荡提取，冷冻30 min后低温离心，提取液经增强型脂质去除净化管(QuEChERS EMR-Lipid)和EMR-Lipid反萃管进一步净化，目标物经极性毛细管柱分离后以多反应监测模式(MRM)检测和基质匹配外标法定量。结果 在0.2～40.0 μg/L范围内线性相关系数均大于0.990，方法检出限及定量限分别为0.03～0.30 μg/kg和0. 1～1.0 μg/kg。三个水平（2.0、5.0和10.0 μg/kg）的加标回收率范围为64.1%～106.1%，相对标准偏差为3.48%～14.55%。结论 本法无需活化、平衡、洗脱步骤，消耗溶剂较少，操作简单，适用于腊肉中N-亚硝胺类化合物的快速检测。

腊肉  /  N-亚硝胺  /  气相色谱-串联质谱法  /  增强型基质去除分散固相萃取法

Objective To establish a method for the rapid detection of 15 N-nitrosamines in bacon by enhanced matrix removal dispersive solid phase extraction combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The samples were extracted with acetonitrile oscillation, frozen for 30 minutes, and then centrifuged at low temperature. The extract was further purified using an enhanced lipid removal purification tube (QuEChERS EMR-lipid) and an EMR-lipid stripping tube. The N-nitrosamines were separated on a polar capillary column, detected in the multiple reaction monitoring (MRM) mode and quantified by the matrix-matched external standard method. Results The linear correlation coefficients of the method were greater than 0.990 in the range of 0.2-40.0 μg/L, with the detection and quantification limits of 0.03-0.30 and 0.1-1.0 μg/kg, respectively. The recoveries were 64.1%-106.1% at three spiked levels (2.0, 5.0, 10.0 μg/kg)，with the relative standard deviations (RSDs) ranging from 3.48% to 14.55%. Conclusion The method does not require activation, equilibration, or elution steps, consumes less solvent, is simple and rapid, and is suitable for the rapid detection of 15 N-nitrosamines in bacon.

Bacon  /  N-nitrosamines  /  Gas chromatography-tandem mass spectrometry  /  Enhanced matrix removal dispersive solid phase extraction
张权, 彭蕾, 吴玉田, 毕珊, 周贻兵, 林野, 龙婷, 陈庆园, 郭华, 刘利亚. QuEChERS净化-气相色谱-串联质谱法快速测定腊肉中15种N-亚硝胺. 现代预防医学, 2025 , 52 (19) : 3620 -3626 . DOI: 10.20043/j.cnki.MPM.202505059
Quan ZHANG, Lei PENG, Yu-tian WU, Shan BI, Yi-bing ZHOU, Ye LIN, Ting LONG, Qing-yuan CHEN, Hua GUO, Li-ya LIU. Determination of 15 N-nitrosamines in bacon by QuEChERS purification and gas chromatography-tandem mass spectrometry[J]. Modern Preventive Medicine, 2025 , 52 (19) : 3620 -3626 . DOI: 10.20043/j.cnki.MPM.202505059
正文
收起
腊肉是我国传统且具有独特风味的肉制品之一,贵州腊肉的制作过程也非常独特，一般选用当地的木材和柴火进行长时间的烟熏，是当地的特色美食之一[1-2]。硝酸盐因具有护色、抑菌、改良肉质等作用，在腊肉的生产加工过程中硝酸盐常被作为主要原材料[3]，由于高盐腌制,以及烟熏、发酵等过程,如果控制不当,亚硝酸盐在酸性条件下会和胺类物质形成致癌物亚硝胺，食用后存在健康隐患。
目前，腊肉中亚硝胺检测主要参照《食品中N-亚硝胺类化合物的测定》（GB 5009.26—2023），但标准中只针对N-二甲基亚硝胺（NDMA）[4]。同时该标准中规定四种检测方法，其中气相色谱-热能分析法虽具有检测快速、灵敏度高等优点，但其价格昂贵，应用范围窄[5]。水蒸气蒸馏法虽净化效果好，缺点是需要的样品量较大，分析周期较长，溶剂消耗量较大，操作繁琐，关键步骤较多[6]。而QuEChERS-气相色谱-质谱法中虽然应用较广，但需要配备大体积程序升温进样口，一般实验室并未配备，因此国家标准检测方法还有待进一步优化。
样品前处理是腊肉检测中的关键和难点[7]，由于其基质复杂，脂肪和蛋白质的含量较高，以及N-亚硝胺化合物都易溶于水、易挥发，导致在检测过程中，经常出现油脂难以净化完全，目标物容易损失，难以准确定量[8-9]。目前，商品化的适用于肉制品的净化材料众多，在肉制品净化方面都有一定的应用[10-11]，本研究通过比较肉制品中目前常用的净化方法，确定最优净化方式并结合GC-MS/MS快速检测腊肉中15种N-亚硝胺化合物，同时对贵州省2024年食品安全风险监测地方专项所收集的130份代表性腊肉样品中N-亚硝胺类化合物进行检测，发现我省腊肉中N-亚硝胺化合物种类，为其他肉制品中N-亚硝胺的测定和法规完善提供参考。
1　材料与方法
收起
1.1　仪器与试剂
Scion-TQ气相色谱质谱联用仪（美国BRUKER公司）；FW100型高速万能粉碎机（天津市泰斯特仪器有限公司）；DMV-16型涡旋混合器（广东省科寅实验室设备有限公司）；Sigma3-18K型台式高速冷冻离心机（德国西格玛公司）。
12种亚硝胺类混合标准溶液（100 mg/L，上海安谱实验科技股份有限公司）；N-亚硝基乙基异丙胺（100 mg/L，纯度>99.8%，曼哈格生物科技有限公司）；N-亚硝基二异丙基胺（10 mg，纯度>99.5%，北方伟业计量技术研究院）；N-亚硝基二异丁胺（100 mg/L，纯度>99.8%）；标准品试剂：乙腈、甲醇、二氯甲烷、乙酸乙酯（色谱纯，纯度均>99.8%，美国TEDIA公司）。Cleanert亚硝胺专用检测管（含分散固相萃取管及Nitro亚硝胺专用净化管，博纳艾杰尔科技有限公司），dSPE亚硝胺检测萃取净化管（含dSPE分散固相萃取管，吡咯烷酮-聚苯乙烯二乙烯基苯净化管，dSPE基质分散固相萃取管，上海安谱实验科技股份有限公司）；PLS-A QuEChERS净化管（150 mg，迪马科技有限公司）；EMR-Lipid dSPE增强型基质去除净化管及反萃管(安捷伦科技有限公司)；Clean-up LPAS多功能净化柱（500 mg/5 ml，北京科德诺斯技术有限公司）；HLB固相萃取柱（200 mg/6 ml，安捷伦科技有限公司）；Captiva EMR-Lipid HF固相萃取柱（600 mg/6 ml，安捷伦科技有限公司）。
1.2　标准溶液配制
将N-亚硝基二异丙基胺标准品用乙腈溶解后通过稀释配制成浓度为100.0 mg/L标准储备液，然后分别吸取3种亚硝胺单个标准溶液及12种亚硝胺类混合标准溶液各1.00 ml，稀释配制成浓度为1.00 μg/ml的15种亚硝胺混合标准储备液，于-18℃保存，用乙腈稀释成0.20 μg/L～40.0 μg/L的标准系列溶液。空白基质溶液经氮气吹干后分别加入1.00 ml上述混合标准工作液复溶后配制成基质匹配混合标准工作溶液系列。
1.3　样品
来自于贵州省2024年食品安全风险监测地方专项，由贵阳市、遵义市、毕节市、黔东南州、铜仁市、黔西南州、黔南州、安顺市、六盘水市9个行政区域大型超市采集的130份腊肉样品。选取250 g以上代表性样品切块后置于粉碎机，捣碎后均匀制样置于玻璃样品瓶中，密封，做好标识，于-18 ℃冷冻保存备用。
1.4　分析条件
HP-INNO Wax毛细管色谱柱(30 m×0.25 mm，0.25 μm)，载气为高纯氦气，碰撞气为高纯氩气；程序升温条件：起始温度为60 ℃，保持1 min，以10 ℃/min速率升高至140 ℃，以5 ℃/min速率升高至150 ℃保持2.0 min，以20 ℃/min升至250 ℃保持7 min，共25.0 min，溶剂延迟时间为4.5 min；进样口温度为250 ℃，流速为1.0 ml/min，不分流进样，进样量为1.0 μl。
质谱离子源温度为200 ℃，传输线温度为250 ℃；电离模式为电子轰击离子化(EI)，电离能量为70 eV；多反应监测模式(MRM)；方法的质谱参数见表1。
1.5　样品前处理
1.5.1　QuEChERS法
称取5.00 g腊肉样品置于50 ml离心管中，加入10 ml水和10 ml乙腈，涡旋1min，加入6颗玻璃珠均质子，再加入提取包（含4.0 g无水硫酸镁和1.0 g氯化钠），高速振荡1 min，以10 000 r/min条件（离心半径9.5 cm）离心5 min，上清液待净化。取不同体积的上述净化液分别加入到对应的净化管中（Agela取1 ml加入Cleanert Nitro净化管，CNW取5ml加入吡咯烷酮-聚苯乙烯二乙烯基苯净化管、净化前需加5 ml水活化，Dima取5ml加入PLS-A净化管），涡旋混匀后8 000 r/min离心5 min，移取全部净化液于基质分散固相萃取管中（Agela可跳过此步骤直接过滤后上机测定，CNW 和Dima萃取管内为1.6 g硫酸镁和0.4 g氯化钠），振荡提取2 min后放入冷冻离心机中，以8 000 r/min（离心半径9.5 cm）条件于4 ℃离心5 min，取上清液约1.0 ml过0.22 μm针式过滤器后上机测定。
1.5.2　EMR-Lipid dSPE法
称取5.00 g腊肉样品于50 ml离心管中，加入10 ml乙腈及6颗玻璃珠，振荡提取3 min后放入-20 ℃冰箱冷冻30 min，振荡提取2 min，以8 000 r/min于0 ℃离心5 min，向增强型基质去除净化管中加入5 ml水，高速振荡1 min，取5 ml提取液加入净化管中，振荡提取2 min，以8 000 r/min（离心半径9.5 cm）条件于0 ℃离心5 min，将离心所得全部上清液倒入15 ml反萃管中，高速振荡2 min，以8 000 r/min（离心半径9.5 cm）条件于0 ℃离心5 min，取上清液约1.0 ml过0.22 μm针式过滤器后上机测定。
1.5.3　SPE法
称取5.00 g腊肉样品于50 ml离心管中，加入10 ml水后高速振荡2 min，加入10 ml乙腈，振荡提取2 min，加入4.0 g无水硫酸镁和1.0g氯化钠，涡旋1 min，以10 000 r/min（离心半径9.5 cm）条件离心5 min，取不同体积的上清液于对应的固相萃取柱上（HLB固相萃取柱上样2 ml，LPAS多功能净化柱上样5 ml后推动柱塞杆以1滴/s的速度收集净化液，Captiva EMR-Lipid HF上样5 ml后依靠重力自流），收集净化液过0.22 μm针式过滤器后上机测定。
2　结果
收起
2.1　提取溶剂的选择
提取溶剂是影响回收率的关键因素，15种N-亚硝胺类化合物的极性较强，多易溶于水、醇、醚等[12]，本研究利用空白腊肉样品加标方式分别比较二氯甲烷、乙腈、乙酸乙酯、甲醇四种提取溶剂的提取效率，结果见图1。
结果表明，当甲醇为提取剂时，盐析后甲醇与水无法分层，无法进行后续操作；当二氯甲烷、乙酸乙酯为提取剂时，N-亚硝基二甲胺、N-亚硝胺吗啉和N-亚硝胺哌啶三种化合物提取回收率较低，其次由于这两种溶剂的极性较弱，易提取出更多油脂，出现严重乳化现象，不利于后续净化过程；当乙腈为提取剂时，目标物回收率较好，且乙腈可沉淀蛋白。因此，选择乙腈为提取溶剂。
2.2　净化方法优化
腊肉样品中存在脂肪、色素、甾醇等杂质，N-亚硝胺化合物易溶于水、易挥发，导致在检测过程中，经常出现油脂难以净化完全[13]。目前，腊肉基质常用的净化方式有QuEChERS、dSPE和SPE法，本研究分别选取7份腊肉空白样品，分别根据“1.5”所述进行样品前处理，在全扫描模式（扫描范围：50～500 m/z）下分别测定，不同净化方式的总离子流色谱图见图2。
由图2可见，CNW QuEChERS、EMR-Lipid dSPE净化法都可有效降低杂质干扰，净化效果较好，最终确定以EMR-Lipid dSPE为净化柱。腊肉中脂肪含量较高，通常可利用冷冻和低温离心的方法使脂肪从提取液中析出并分离，一般冷冻时间30 min后除脂效果会更加彻底[14]，本研究利用腊肉空白样品，按照“1.5.2”节进行操作，在全扫描模式（扫描范围：50～500 m/z）测定，分别对冷冻/低温离心（-20 ℃冰箱冷冻30 min，0 ℃下离心）与常温下操作的除脂效果进行比较，结果表明，腊肉样品经冷冻过后的除脂效果较好，样品全扫谱图基线较平，杂质干扰较小，所以后续实验中样品提取后在放入-20 ℃冰箱冷冻30 min，并采用低温离心。
2.3　色谱柱的选择
15种N-亚硝胺化合物分子量和极性差异较大[15]，分别比较了DB-5MS、DB-1701MS和HP-INNOWAX色谱柱的分离效果。结果表明，DB-5MS柱和DB-1701MS柱的分离效果较差，特别是NDMA的保留时间短，与溶剂峰近，有拖尾现象且易受杂质峰干扰；15种目标物在HP-INNOWAX柱峰形较好且在25 min内都能有很好的分离，腊肉加标样品的总离子流色谱图见图3。
2.4　定量方法
N-亚硝胺类化合物检测通常是按参考文献4中以N-二甲基亚硝胺-D6为内标，其N-二甲基亚硝胺-D6的定性及定量离子对参数（74.0>44.0，碰撞能量3 V；74.0>42.1，碰撞能量19 V），但实验中发现，如按该参数设置，N-二甲基亚硝胺和N-二甲基亚硝胺-D6中存在离子串扰现象，严重影响定量的准确性，且N-二甲基亚硝胺分子量较小，特异性的离子对参数较少，所以采用外标法定量。
2.5　基质效应
基质效应一直影响质谱分析定量的准确性[16-17]，通常基质效应以相对响应值法来评价，即基质标准溶液的峰面积与溶剂标准溶液的峰面积的比值乘以100%。当基质效应<80%时，表现为基质抑制效应；当基质效应在 80%～120%范围时，基质效应可忽略不计；当基质效应>120%时，表现为基质增强效应[18-19]。本研究分别取5个批次不同厂家腊肉样品进行混匀制备成混合样品基质，在其中添加混合标准（5 μg/kg）后分别按照“1.5”中的7种方式进行操作，每种方式平行测定5次后计算基质效应。结果表明，腊肉基质效应干扰比较严重，未经净化操作很难进行定量分析。当采用QuEChERS（Agela、Dima）、SPE（LPAS、HLB）净化时15种目标物中基质效应在0～80.0%分别有2、2、2和1个，只有经EMR-Lipid dSPE净化后的15种目标物的基质效应均在80.1%～120.0%，能更有效地减小基质效应。
2.6　线性范围、检出限、定量限及加标回收率
取空白样品分别加入6个不同浓度的混合标准溶液，按本法测定。以3倍和10倍信噪比（S/N）对应的含量确定方法检出限和定量限。为验证方法的准确度与精密度，取数份腊肉空白样品进行添加回收实验，添加水平为2.00、5.00和10.0 μg/kg，放置隔夜后平行测定6次。结果表明，15种目标物线性关系良好(相关系数r2>0. 99)。方法检出限及定量限分别为0.03～0.3 μg/kg和0.1～1.0 μg/kg；加标回收率为64.1%～106.1%，相对标准偏差（relative standard deviation，RSD）为3.48%～14.55%。见表2。
2.7　实际样品的检测
采用本法测定了130份腊肉样品，结果表明，在6份腊肉样品中各检出至少1种亚硝胺化合物残留，检出率最高的分别是NDMA、NDIBA、NMPHA及NDPHA，其中检出NDMA的残留量在0～10.0 μg/kg，超过GB 2762—2022《食品安全国家标准 食品中污染物限量》中的限量值（NDMA<3.0 μg/kg），这可能与腊肉独特的加工工艺有关[20]。
3　结论
收起
本研究建立了增强型基质去除技术结合GC-MS/MS快速筛查贵州产腊肉中15种挥发性N-亚硝胺化合物残留的方法。该方法前处理操作便捷，基质净化效果较好，易于推广，适合腊肉中挥发性N-亚硝胺化合物的批量检测。
基金
收起
  • 贵州省卫生健康委科学技术基金项目(gzwkj2024-224)
  • 贵州疾控博士工作室(bsgzs2025-01)
  • 贵州省卫生健康委省级重点建设学科项目
文献
收起
参考文献 引证文献
排序方式：
[1]
王洪伟，索化夷，张玉，等.感官评价和GC-MS结合偏最小二乘回归法分析酚类化合物对腊肉烟熏风味的贡献[J].食品与发酵工业，2019，45（21）：244-249.
Wang HW, Suo HY, Zhang Y, et al. PLSR analysis and sensory evaluation of GC-MS result of phenolic compounds contribution to smoked flavor in Chinese bacon[J]. Food and Fermentation Industries, 2019, 45(21): 244-249.(In Chinese)
[2]
董亚云，张迎阳，杨天志，等.基于HS-SPME-GC×GC-TOFMS技术分析传统干腌腊肉加工过程中的挥发性有机物[J].肉类研究，2024，38（3）：30-38.
Dong YY, Zhang YY, Yang TZ, et al. HS-SPME-GC×GC-TOFMS analysis of volatile flavor compounds in traditional Dry-Cured bacon during processing[J]. Meat Research, 2024, 38(3): 30-38.(In Chinese)
[3]
朱新鹏，梁如，张伟艺，等.腊肉的食用安全性及其有害物质控制[J].食品安全质量检测学报，2020，11（19）：6829-6834.
Zhu XP, Liang R, Zhang WY, et al. Food safety of cured meat and control of its harmful components[J]. Journal of Food Safety & Quality, 2020, 11(19): 6829-6834.(In Chinese)
[4]
中华人民共和国国家卫生和计划生育委员会， 国家食品药品监督管理总局.GB 5009.26-2023 食品安全国家标准食品中N-亚硝胺类化合物的测定[S].北京：中国标准出版社，2023.
The National Health and Family Planning Commission of the People’s Republic of China, the China Food and Drug Administration. GB 5009.26-2023 National Food Safety Standard Determination of N-nitrosamines in food[S]. Beijing: Standards Press of China, 2023.(In Chinese)
[5]
李芳芳，梁秀清，王艳丽，等.氢氧化钡处理-分散液液微萃取结合气相色谱-串联质谱法测定肉制品中9种N-亚硝胺[J].食品安全质量检测学报，2023，14（4）：137-144.
Li FF, Liang XQ, Wang YL, et al. Determination of 9 kinds of N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry with Barium hydroxide treatment followed dispersive liquid-liquid microextraction[J]. Journal of Food Safety and Quality, 2023, 14(4):137-144.(In Chinese)
[6]
王艳丽，梁秀清，陈倩倩，等.通过式固相萃取-气相色谱-串联质谱法测定动物源性食品中11种N-亚硝胺类化合物[J].肉类研究，2023，37（3）：33-39.
Wang YL, Liang XQ, Chen QQ, et al. Determination of eleven N-Nitrosamines in animal derived foods by gas Chromatography-Tandem mass spectrometry after Pass-Through solid phase extraction[J]. Meat Research, 2023, 37(3): 33-39.(In Chinese)
[7]
何健，赵舰，唐晓琴，等.加速溶剂萃取/凝胶渗透色谱净化-气质联用法测定烟熏腊肉中24种多环芳烃[J].现代预防医学，2019，46（1）：126-131.
He J, Zhao J, Tang XQ, et al. Determination of 24 Polycyclic Aromatic Hydrocarbons in Smoked meat by ASE extraction-GPC purification combined with gas chromatography-mass spectrometry[J]. Modern Preventive Medicine, 2019, 46(1): 126-131.(In Chinese)
[8]
孔祥一，庄丽丽，方恩华，等.QuEChERS-同位素稀释-气相色谱-串联质谱法测定动物源性食品中9种N-亚硝胺类化合物[J].色谱，2021，39（1）：96-103.
Kong XY, Zhuang LL, Fang EH, et al. Determination of nine N-nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2021, 39(1): 96-103.(In Chinese)
[9]
朱萌萌，叶群，周婷婷，等. 气相色谱-串联质谱法测定肉制品中10种挥发性N-亚硝胺类化合物[J].色谱，2019，37（2）：207-215.
Zhu MM, Ye Q, Zhou TT, et al. Determination of 10 volatile N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(2): 207-215.(In Chinese)
[10]
夏寒，仝凯旋，朱浙辉，等.一步式QuEChERS-气相色谱-三重四极杆质谱法快速测定风干牦牛肉中15种N-亚硝胺[J].色谱，2024，42（5）：465-473.
Xia H, Tong KX, Zhu ZH, et al. Rapid determination of 15 N-nitrosamines in air-dried yak meat using one-step QuEChERS-gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2024, 42(5): 465-473.(In Chinese)
[11]
沈昌莹，苏骏敏，莫淑梅，等.QuEChERS结合GC-MS/MS测定腌腊肉制品中N-二甲基亚硝胺[J].食品工业，2023，44（7）：291-294.
Shen CY, Su JM, Mo SM, et al. Determination of N-Dimethylnitrosamine in preserved meat products by QuEChERS combined with GC-MS/MS[J]. The Food Industry, 2023, 44(7): 291-294.(In Chinese)
[12]
袁娅，周鸿，宋迎春，等.同位素稀释-气相色谱串联质谱法测定啤酒中4种亚硝胺类化合物[J].现代预防医学，2021，48（9）：1667-1671.
Yuan Y, Zhou H, Song YC, et al. Determination of four N - nitrosamines in beer by isotope dilutiongas Chromatography-tandem mass spectrometry[J].Modern Preventive Medicine, 2021, 48(9): 1667-1671.(In Chinese)
[13]
马兴，肖亚兵，陈颖，等.快速水蒸气蒸馏-气相色谱热能分析仪法测定肉制品和水产制品中13种N-亚硝胺[J].食品研究与开发，2020，41（20）：198-203.
Ma X, Xiao YB, Chen Y, et al. Determination of 13 N-Nitrosamines in meat and aquatic products by gas Chromatography-Thermal energy analyzer with rapid steam distillation[J]. Food Research and Development,2020, 41(20): 198-203.(In Chinese)
[14]
戴裕杰，关荣发，黄海智，等.改进的QuEChERS-气相色谱-质谱法测定中式腊肉中8种挥发性N-亚硝胺[J].食品科学，2021，42（20）：266-273.
Dai YJ, Guan RF, Huang HZ, et al. Determination of eight volatile N-Nitrosamines in Chinese bacon by modified QuEChERS and gas Chromatography-Mass spectrometry[J]. Food Science, 2021, 42(20): 266-273.(In Chinese)
[15]
张静，赖俊敏，李思源，等.气相色谱-三重四极杆质谱法测定染发剂中14种N-亚硝胺类化合物[J].日用化学工业，2023，53（11）：1334-1339.
Zhang J, Lai JM, Li SY, et al. Determination of 14 N-nitrosamines in hair dyes using gas chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1334-1339.(In Chinese)
[16]
李林竹，马凯，杨昌彪，等.QuEChERS-超高效液相色谱-串联质谱法检测腌腊肉中四种常用香料[J].食品与发酵工业，2021，47（16）：247-252.
Li LZ, Ma K, Yang CB, et al. Determination of four spices in cured meat samples by QuEChERS coupled with ultra-performance liquid chomatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021, 47(16): 247-252.(In Chinese)
[17]
夏仕青，吴桃丽，薛云，等.QuEChERs结合气相色谱-串联质谱法测定不同果蔬基质中33种有机磷类农药残留量[J].现代预防医学，2024，51（3）：523-528.
Xia SQ, Wu TL, Xue Y, et al. Determination of 33 organophosphorus pesticide residues in different fruit and vegetable substrates by QuEChERs combined with gas chromatogra-phy-tandem mass spectrometry[J].Modern Preventive Medicine, 2024, 51(3): 523-528.(In Chinese)
[18]
张艳，曲良娇，凌莉，等.UPLC-MS/MS法同时测定蔬菜中11种有机磷和10种新烟碱类杀虫剂[J].现代预防医学，2024，51（4）：722-727.
Zhang Y, Qu LJ, Ling L, et al. Simultaneous determination of eleven organophosphorus and ten neonicotinoid pesticide in vegetables by UPLC-MS/MS[J]. Modern Preventive Medicine, 2024, 51(4): 722-727.(In Chinese)
[19]
李四生，张维，章剑，等.QuEChERS-超高效液相色谱-三重四级杆质谱法同时测定尿液和血液中22种植物毒素[J].现代预防医学，2024，51（4）：713-721.
Li SS, Zhang W, Zhang J, et al. Simultaneous determination of 22 phytotoxins in urine/blood by QuEChERS-Ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. Modern Preventive Medicine, 2024, 51(4): 713-721.(In Chinese)
[20]
范小宁，师希雄，包晓明，等.陇西腊肉加工过程中亚硝胺与理化指标的相关性分析[J].食品与发酵工业，2024，50（5）：226-233.
Fan XN, Shi XX, Bao XM, et al. Correlation analysis of nitrosamines and physical and chemical indexes during Longxi bacon processing[J]. Food and Fermentation Industries, 2024, 50(5): 226-233.(In Chinese)
2025年第52卷第19期
PDF下载
110
51
引用本文
BibTeX
文章信息
doi: 10.20043/j.cnki.MPM.202505059
  • 接收时间：2025-05-05
  • 首发时间：2026-03-17
  • 出版时间：2025-10-10
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2025-05-05
基金
贵州省卫生健康委科学技术基金项目(gzwkj2024-224)
贵州疾控博士工作室(bsgzs2025-01)
贵州省卫生健康委省级重点建设学科项目
作者信息
    1.贵州省疾病预防控制中心，贵州 贵阳 550004
    2.铜仁市疾病预防控制中心

通讯作者:

刘利亚，E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/xdyfyx/CN/10.20043/j.cnki.MPM.202505059
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持