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Article(id=1240651444378988602, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1240651438955754377, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202402040, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1706889600000, receivedDateStr=2024-02-03, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1773723954528, onlineDateStr=2026-03-17, pubDate=1719244800000, pubDateStr=2024-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1773723954528, onlineIssueDateStr=2026-03-17, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1773723954528, creator=13701087609, updateTime=1773723954528, updator=13701087609, issue=Issue{id=1240651438955754377, tenantId=1146029695717560320, journalId=1227665162245664772, year='2024', volume='51', issue='12', pageStart='2113', pageEnd='2912', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1773723953236, creator=13701087609, updateTime=1773723953236, updator=13701087609, preIssue=null, nextIssue=null, ext=null, issueFiles=null}, startPage=2248, endPage=2252, ext={EN=ArticleExt(id=1240651445461119137, articleId=1240651444378988602, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Expression and clinical significance of protein O-fucosyltransferase 1 in non-small cell lung cancer, columnId=1228016572065837304, journalTitle=Modern Preventive Medicine, columnName=Experimental Technology and Applications, runingTitle=null, highlight=null, articleAbstract=
Objective

To investigate the expression and clinical significance of protein-O-fucosyltransferase 1 in non-small cell lung cancer (NSCLC), so as to provide a reference for the diagnosis and treatment of NSCLC.

Methods

The expression of POFUT1in NSCLC was determined by TCGA, UALCAN database and immunohistochemistry. Based on the clinical data form TCGA database were extracted for survival analysis by Kaplan-Meier method, and analysis of the effect of POFUT1 mRNA expression level on prognosis of patients with NSCLC.

Results

POFUT1 was highly expression in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues (all P<0.05). The expression level of POFUT1 mRNA in LUAD was correlated with clinical stage (P<0.001) and that in LUSC was correlated with tumor size (P<0.001). COX regression analysis showed that high mRNA expression of POFUT1 (P=0.012), tumor size, clinical stage and lymph node metastasis were associated with the prognosis of LUAD patients (all P<0.001), and clinical stage III-IV was an independent risk factor for LUAD patients. Tumor size (P=0.001), clinical stage III-IV (P=0.006) and high expression of POFUT1 mRNA (P=0.049) were associated with the prognosis of LUSC patients, and tumor size greater than 5cm and high expression of POFUT1 were independent risk factors for LUSC patients. The survival analysis result showed that the survival rate of patients with high expression of POFUT1 was significantly lower than that of patients with low expression.

Conclusion

POFUT1 is highly expressed in NSCLC tissues, and high POFUT1 expression is correlated with poor prognosis in patients. POFUT1 can serve as a biomarker for prognostic evaluation of NSCLC.

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目的

探讨非小细胞肺癌中蛋白-O-岩藻糖基转移酶1的表达及其临床意义，为非小细胞肺癌的诊断和治疗提供参考依据。

方法

通过TCGA、UALCAN数据库和免疫组化技术探讨POFUT1 mRNA和POFUT1蛋白在非小细胞肺癌的表达情况。提取TCGA数据库的临床数据，采用Kaplan-Meier法进行生存分析，采用COX比例风险回归模型分析POFUT1 mRNA表达水平对非小细胞肺癌患者预后的影响。

结果

POFUT1 mRNA和POFUT1蛋白在肺腺癌和肺鳞癌组织中均高表达（均P<0.05）。肺腺癌中POFUT1 mRNA的表达水平与临床分期相关（P<0.001），肺鳞癌中POFUT1 mRNA的表达与肿瘤直径相关（P<0.001）。COX回归分析结果显示，POFUT1 mRNA高表达（P=0.012）、肿瘤直径、临床分期、淋巴结转移与肺腺癌患者预后相关（均P<0.001），临床III-IV期是肺腺癌患者独立危险因素。肿瘤直径（P=0.001）、临床III-IV期（P=0.006）和POFUT1 mRNA高表达（P=0.049）与肺鳞癌患者预后相关，肿瘤直径大于5cm和POFUT1高表达是肺鳞癌患者独立危险因素。生存分析结果显示POFUT1高表达的非小细胞肺癌患者生存率显著低于低表达患者。

结论

POFUT1在非小细胞肺癌组织中高表达，且其高表达与患者不良预后相关，可作为非小细胞肺癌预后评估的生物标志物。

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郭艳琳，E-mail：
, copyrightStatement=本刊刊出的所有文章不代表中华预防医学会和本刊编委会的观点，除非特别声明。, copyrightOwner=中华预防医学会和四川大学华西公共卫生学院, extLink=null, articleAbsUrl=null, sourceXml=km3feXJtA/cGs6rdlgOL9A==, magXml=LaBiltpqYR/1J3Nlj5pOmg==, pdfUrl=null, pdf=uMkApdCb5Yzo2s0O3yg8JA==, pdfFileSize=779444, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=uzHuOOlfxm+XhZIZK7j+EQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=/Fvx+VASaKXHRcP1fkBqig==, mapNumber=null, authorCompany=null, fund=null, authors=

王晓慧（1997—），女，硕士在读，研究方向：肿瘤病理

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王晓慧（1997—），女，硕士在读，研究方向：肿瘤病理

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王晓慧（1997—），女，硕士在读，研究方向：肿瘤病理

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tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, orderNo=5, keyword=预后)], refs=[Reference(id=1240651457767207658, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2022, volume=135, issue=5, pageStart=584, pageEnd=590, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Xia CF, Dong XS, Li H, journalName=Chinese Medical Journal, refType=null, unstructuredReference=Xia CF, Dong XS, Li H, et al. Cancer statistics in China and United States, 2022: profiles, trends, and determinants[J]. Chinese Medical Journal, 2022, 135(5): 584-590., articleTitle=Cancer statistics in China and United States, 2022: profiles, trends, and determinants, refAbstract=null), Reference(id=1240651457851093741, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2022, volume=40, issue=6, pageStart=611, pageEnd=625, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=Tan AC, Tan DSW, journalName=Journal of Clinical Oncology, refType=null, unstructuredReference=Tan AC, Tan DSW. Targeted therapies for lung cancer patients with oncogenic driver molecular alterations[J]. Journal of Clinical Oncology, 2022, 40(6): 611-625., articleTitle=Targeted therapies for lung cancer patients with oncogenic driver molecular alterations, refAbstract=null), Reference(id=1240651457964339956, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2023, volume=66, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=Bangarh R, Khatana C, Kaur S, journalName=Biotechnology Advances, refType=null, unstructuredReference=Bangarh R, Khatana C, Kaur S, et al. Aberrant protein glycosylation: Implications on diagnosis and immunotherapy[J]. Biotechnology Advances, 2023, 66: 108149., articleTitle=Aberrant protein glycosylation: Implications on diagnosis and immunotherapy, refAbstract=null), Reference(id=1240651458102751992, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2023, volume=4, issue=3, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=Zhong Q, Xiao XN, Qiu YJ, journalName=MedComm, refType=null, unstructuredReference=Zhong Q, Xiao XN, Qiu YJ, et al. Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications[J]. MedComm, 2023, 4(3): e261., articleTitle=Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications, refAbstract=null), Reference(id=1240651458178249468, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2023, volume=28, issue=1, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=Lv YX, Zhang ZD, Tian S, journalName=Drug Discovery Today, refType=null, unstructuredReference=Lv YX, Zhang ZD, Tian S, et al. Therapeutic potential of fucosyltransferases in cancer and recent development of targeted inhibitors[J]. Drug Discovery Today, 2023, 28(1): 103394., articleTitle=Therapeutic potential of fucosyltransferases in cancer and recent development of targeted inhibitors, refAbstract=null), Reference(id=1240651458295689984, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2021, volume=53, issue=5, pageStart=621, pageEnd=632, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=Li Q, Wang J, Ma XD, journalName=Journal of Bioenergetics and Biomembranes, refType=null, unstructuredReference=Li Q, Wang J, Ma XD, et al. POFUT1 acts as a tumor promoter in glioblastoma by enhancing the activation of Notch signaling[J]. Journal of Bioenergetics and Biomembranes, 2021, 53(5): 621-632., articleTitle=POFUT1 acts as a tumor promoter in glioblastoma by enhancing the activation of Notch signaling, refAbstract=null), Reference(id=1240651458400547588, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2024, volume=27, issue=1, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=Long F, Tian BN, Li L, journalName=iScience, refType=null, unstructuredReference=Long F, Tian BN, Li L, et al. CircPOFUT1 fosters colorectal cancer metastasis and chemoresistance via decoying miR-653-5p/E2F7/WDR66 axis and stabilizing BMI1[J]. iScience, 2024, 27(1): 108729., articleTitle=CircPOFUT1 fosters colorectal cancer metastasis and chemoresistance via decoying miR-653-5p/E2F7/WDR66 axis and stabilizing BMI1, refAbstract=null), Reference(id=1240651458492822277, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2023, volume=86, issue=9, pageStart=806, pageEnd=817, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=Dong S, Wang ZR, Xiong WJ, journalName=Journal of the Chinese Medical Association: JCMA, refType=null, unstructuredReference=Dong S, Wang ZR, Xiong WJ. POFUT1 promotes gastric cancer progression through Notch/Wnt dual signaling pathways dependent on the parafibromin-NICD1-catenin complex[J]. Journal of the Chinese Medical Association: JCMA, 2023, 86(9): 806-817., articleTitle=POFUT1 promotes gastric cancer progression through Notch/Wnt dual signaling pathways dependent on the parafibromin-NICD1-catenin complex, refAbstract=null), Reference(id=1240651458618651399, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2014, volume=9, issue=5, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=Varghese F, Bukhari AB, Malhotra R, journalName=PLOS One, refType=null, unstructuredReference=Varghese F, Bukhari AB, Malhotra R, et al. IHC profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples[J]. PLOS One, 2014, 9(5): e96801., articleTitle=IHC profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples, refAbstract=null), Reference(id=1240651458723509003, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2021, volume=189, issue=null, pageStart=279, pageEnd=291, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=Gao GW, Li C, Fan WG, journalName=International Journal of Biological Macromolecules, refType=null, unstructuredReference=Gao GW, Li C, Fan WG, et al. Brilliant glycans and glycosylation: Seq and ye shall find[J]. International Journal of Biological Macromolecules, 2021, 189: 279-291., articleTitle=Brilliant glycans and glycosylation: Seq and ye shall find, refAbstract=null), Reference(id=1240651458824172300, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2018, volume=10, issue=11, pageStart=411, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=Chabanais J, Labrousse F, Chaunavel A, journalName=Cancers, refType=null, unstructuredReference=Chabanais J, Labrousse F, Chaunavel A, et al. POFUT1 as a promising novel biomarker of colorectal cancer[J]. Cancers, 2018, 10(11): 411., articleTitle=POFUT1 as a promising novel biomarker of colorectal cancer, refAbstract=null), Reference(id=1240651458882892557, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2018, volume=144, issue=11, pageStart=2109, pageEnd=2115, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=Leng QX, Tsou JH, Zhan M, journalName=Journal of Cancer Research and Clinical Oncology, refType=null, unstructuredReference=Leng QX, Tsou JH, Zhan M, et al. Fucosylation genes as circulating biomarkers for lung cancer[J]. Journal of Cancer Research and Clinical Oncology, 2018, 144(11): 2109-2115., articleTitle=Fucosylation genes as circulating biomarkers for lung cancer, refAbstract=null), Reference(id=1240651458983555857, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, doi=null, pmid=null, pmcid=null, year=2013, volume=43, issue=6, pageStart=1864, pageEnd=1870, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=Yokota S, Ogawara K, Kimura R, journalName=International Journal of Oncology, refType=null, unstructuredReference=Yokota S,Ogawara K, Kimura R, et al. Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer[J]. International Journal of Oncology, 2013, 43(6): 1864-1870., articleTitle=Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer, refAbstract=null)], funds=[Fund(id=1240651457536520924, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, awardId=20210302124185, language=CN, fundingSource=山西省基础研究计划(20210302124185), fundOrder=null, country=null), Fund(id=1240651457612018402, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, awardId=2021-082, language=CN, fundingSource=山西省回国留学人员科研资助项目(2021-082), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1240651449030472034, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, xref=1., ext=[AuthorCompanyExt(id=1240651449034666339, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, companyId=1240651449030472034, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Department of Pathology, Shanxi Medical University, Taiyuan, Shanxi 030001, China), AuthorCompanyExt(id=1240651449051443559, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, companyId=1240651449030472034, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西医科大学基础医学院病理教研室，山西 太原 030001)]), AuthorCompany(id=1240651449273741684, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, xref=2., ext=[AuthorCompanyExt(id=1240651449282130296, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, companyId=1240651449273741684, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西医科大学基础医学院解剖教研室)])], figs=[ArticleFig(id=1240651454629868174, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Fig.1, caption=The difference of POFUT1 mRNA expression between LUAD(A)and LUSC(B)and normal tissue by TCGA database, figureFileSmall=oNKBtMoQWlBNfxJW2XY4zg==, figureFileBig=uzHuOOlfxm+XhZIZK7j+EQ==, tableContent=null), ArticleFig(id=1240651454705365652, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=图1, caption=TCGA中比较LUAD（A）和LUSC（B）与正常肺组织中POFUT1 mRNA表达差异

注：***P<0.001。

, figureFileSmall=oNKBtMoQWlBNfxJW2XY4zg==, figureFileBig=uzHuOOlfxm+XhZIZK7j+EQ==, tableContent=null), ArticleFig(id=1240651454940246688, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Fig.2, caption=The difference of POFUT1 protein expression between LUAD(A)and LUSC(B)andnormaltissue by UALCAN database, figureFileSmall=PX9Yk+vfcoC2GhL9W27unQ==, figureFileBig=c9R2OauI7vARTNCb/RqCrw==, tableContent=null), ArticleFig(id=1240651455066075815, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=图2, caption=UALCAN数据库比较LUAD（A）和LUSC（B）与正常肺组织中POFUT1蛋白表达差异

注：**P<0.01；*P<0.05。

, figureFileSmall=PX9Yk+vfcoC2GhL9W27unQ==, figureFileBig=c9R2OauI7vARTNCb/RqCrw==, tableContent=null), ArticleFig(id=1240651455166739116, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Fig.3, caption=Immunohistochemical examination of POFUT1 expression in LUAD and LUSC (A)Representative immunohistochemical staining results of POFUT1; scale bar: 500 μm at low magnification, 50 μm at high magnification;(B)Quantitative analysis of POFUT1protein expression level, figureFileSmall=6Gcfmp8R7i/dM3Wp1HJ7yA==, figureFileBig=TDj9lvCBczGanBrIwJV+oQ==, tableContent=null), ArticleFig(id=1240651456701854385, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=图3, caption=免疫组织化学法检测POFUT1蛋白在LUAD、LUSC及正常肺组织的表达（A）POFUT1代表性免疫组织化学染色；比例尺：低倍500 μm，高倍50 μm;（B）POFUT1蛋白表达水平定量分析

注：***P<0.001。

, figureFileSmall=6Gcfmp8R7i/dM3Wp1HJ7yA==, figureFileBig=TDj9lvCBczGanBrIwJV+oQ==, tableContent=null), ArticleFig(id=1240651456794129078, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Fig.4, caption=Relationship assessment between POFUT1 expression and prognosis in LUAD(A) and LUSC(B) by TCGA database, figureFileSmall=6g2aeOlFbtYb6vt0ZQThHw==, figureFileBig=FvPQrlBVLBeqLQeyRV5O3A==, tableContent=null), ArticleFig(id=1240651456886403771, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=图4, caption=TCGA数据库评估LUAD（A）和LUSC（B）POFUT1表达与预后的相关性, figureFileSmall=6g2aeOlFbtYb6vt0ZQThHw==, figureFileBig=FvPQrlBVLBeqLQeyRV5O3A==, tableContent=null), ArticleFig(id=1240651456974484159, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Table 1, caption=

Relationship between POFUT1 mRNA expression and clinical characteristics of patients with NSCLC

, figureFileSmall=null, figureFileBig=null, tableContent=
临床特征例数POFUT1在肺腺癌的表达例数POFUT1在肺鳞癌的表达
低表达(%)高表达(%)P值低表达(%)高表达(%)P值
年龄0.1370.784
<6013862(44.9)76(55.1)9077(85.6)13 (14.4)
≥60356134(37.6)222(62.4)397344(86.6)53(13.4)
性别0.9650.163
女276110(39.9)166(60.1)129116(89.9)13(10.1)
男23794(39.7)143(60.3)367312(85.0)55(15.0)
吸烟史0.5700.603
否322125(38.8)197(61.2)277241(87.0)36(13.0)
是19179(41.3)112(58.7)219187(85.4)32(14.6)
临床分期0.0000.359
Ⅰ期275157(57.1)118(42.9)241210(87.1)31(12.9)
Ⅱ期12127(22.3)94(77.7)160133(83.1)27(16.9)
Ⅲ、Ⅳ期11015(13.6)95 (86.4)9181(89.0)10(11.0)
肿瘤直径（厘米）0.8860.000
<5446177(39.7)269(60.3)402375(93.3)27(6.7)
≥56426(40.6)38(59.4)9431(33.0)63(67.0)
淋巴结转移0.2240.408
否329137(41.6)192(58.4)315269(85.4)46(14.6)
是17262(36.0)110(64.0)176155(88.1)21(11.9)
), ArticleFig(id=1240651457054175939, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=表1, caption=

TCGA数据中POFUT1 mRNA表达与NSCLC患者临床特征的关系

, figureFileSmall=null, figureFileBig=null, tableContent=
临床特征例数POFUT1在肺腺癌的表达例数POFUT1在肺鳞癌的表达
低表达(%)高表达(%)P值低表达(%)高表达(%)P值
年龄0.1370.784
<6013862(44.9)76(55.1)9077(85.6)13 (14.4)
≥60356134(37.6)222(62.4)397344(86.6)53(13.4)
性别0.9650.163
女276110(39.9)166(60.1)129116(89.9)13(10.1)
男23794(39.7)143(60.3)367312(85.0)55(15.0)
吸烟史0.5700.603
否322125(38.8)197(61.2)277241(87.0)36(13.0)
是19179(41.3)112(58.7)219187(85.4)32(14.6)
临床分期0.0000.359
Ⅰ期275157(57.1)118(42.9)241210(87.1)31(12.9)
Ⅱ期12127(22.3)94(77.7)160133(83.1)27(16.9)
Ⅲ、Ⅳ期11015(13.6)95 (86.4)9181(89.0)10(11.0)
肿瘤直径（厘米）0.8860.000
<5446177(39.7)269(60.3)402375(93.3)27(6.7)
≥56426(40.6)38(59.4)9431(33.0)63(67.0)
淋巴结转移0.2240.408
否329137(41.6)192(58.4)315269(85.4)46(14.6)
是17262(36.0)110(64.0)176155(88.1)21(11.9)
), ArticleFig(id=1240651457138062023, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Table 2, caption=

COX regression analysis of lung adenocarcinoma patients in TCGA

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变量单因素分析多因素分析
HR95%CIP值HR95%CIP值
年龄≥60vs<601.0560.766~1.4790.7091.1150.793~1.5700.534
性别男 vs 女1.0760.808~1.4340.6151.0300.759~1.3970.850
吸烟史是 vs 否1.1070.817~1.4980.5121.3230.957~1.8290.090
肿瘤直径（厘米）>5 vs≤52.3201.592~3.379<0.0011.5140.953~2.4050.079
临床分期ⅡvsⅠ2.2961.604~3.286<0.0011.5380.852~2.7780.153
Ⅲ-ⅣvsⅠ3.4842.467~4.920<0.0011.9561.000~3.8240.050
淋巴结转移是vs 否2.4901.861~3.332<0.0011.5890.932~2.7090.089
POFUT1高vs 低1.4741.087~1.9990.0121.3060.949~1.7960.101
), ArticleFig(id=1240651457247113934, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=表2, caption=

TCGA肺腺癌患者COX回归分析

, figureFileSmall=null, figureFileBig=null, tableContent=
变量单因素分析多因素分析
HR95%CIP值HR95%CIP值
年龄≥60vs<601.0560.766~1.4790.7091.1150.793~1.5700.534
性别男 vs 女1.0760.808~1.4340.6151.0300.759~1.3970.850
吸烟史是 vs 否1.1070.817~1.4980.5121.3230.957~1.8290.090
肿瘤直径（厘米）>5 vs≤52.3201.592~3.379<0.0011.5140.953~2.4050.079
临床分期ⅡvsⅠ2.2961.604~3.286<0.0011.5380.852~2.7780.153
Ⅲ-ⅣvsⅠ3.4842.467~4.920<0.0011.9561.000~3.8240.050
淋巴结转移是vs 否2.4901.861~3.332<0.0011.5890.932~2.7090.089
POFUT1高vs 低1.4741.087~1.9990.0121.3060.949~1.7960.101
), ArticleFig(id=1240651457339388626, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=EN, label=Table 3, caption=

COX regression analysis of lung squamous cell carcinoma patients in TCGA

, figureFileSmall=null, figureFileBig=null, tableContent=
变量单因素分析多因素分析
HR95%CIP值HR95%CIP值
年龄≥60vs<601.2380.841~2.8230.2801.2290.831~1.8190.302
性别男 vs 女1.1880.865~1.6320.2881.1680.845~1.6140.347
吸烟史是 vs 否0.8780.669~1.1520.3480.9410.710~1.2460.669
肿瘤直径（厘米）>5 vs≤51.6851.223~2.3210.0011.7711.272~2.4650.001
临床分期ⅡvsⅠ1.1960.873~1.6390.2641.1830.724~1.9330.576
Ⅲ-ⅣvsⅠ1.6191.148~2.2840.0060.9290.637~1.3560.704
淋巴结转移是 vs 否1.1730.890~1.5470.2580.8420.629~1.1260.246
POFUT1高 vs 低1.4171.001~2.0060.0491.4911.041~2.1370.029
), ArticleFig(id=1240651457431663320, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1240651444378988602, language=CN, label=表3, caption=

TCGA肺鳞癌患者COX回归分析

, figureFileSmall=null, figureFileBig=null, tableContent=
变量单因素分析多因素分析
HR95%CIP值HR95%CIP值
年龄≥60vs<601.2380.841~2.8230.2801.2290.831~1.8190.302
性别男 vs 女1.1880.865~1.6320.2881.1680.845~1.6140.347
吸烟史是 vs 否0.8780.669~1.1520.3480.9410.710~1.2460.669
肿瘤直径（厘米）>5 vs≤51.6851.223~2.3210.0011.7711.272~2.4650.001
临床分期ⅡvsⅠ1.1960.873~1.6390.2641.1830.724~1.9330.576
Ⅲ-ⅣvsⅠ1.6191.148~2.2840.0060.9290.637~1.3560.704
淋巴结转移是 vs 否1.1730.890~1.5470.2580.8420.629~1.1260.246
POFUT1高 vs 低1.4171.001~2.0060.0491.4911.041~2.1370.029
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蛋白-O-岩藻糖基转移酶1在非小细胞肺癌中的表达及临床意义
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王晓慧 1 , 李明泰 1 , 高绿叶 1 , 郝艳龙 1 , 冯雪飞 1 , 翟元芳 2 , 郭艳琳 1
现代预防医学 | 实验技术及其应用 2024,51(12): 2248-2252
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现代预防医学 | 实验技术及其应用 2024, 51(12): 2248-2252
蛋白-O-岩藻糖基转移酶1在非小细胞肺癌中的表达及临床意义
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王晓慧1, 李明泰1, 高绿叶1, 郝艳龙1, 冯雪飞1, 翟元芳2, 郭艳琳1
作者信息
  • 1.山西医科大学基础医学院病理教研室，山西 太原 030001
  • 2.山西医科大学基础医学院解剖教研室

    • 王晓慧（1997—），女，硕士在读，研究方向：肿瘤病理

通讯作者:

郭艳琳，E-mail：
Expression and clinical significance of protein O-fucosyltransferase 1 in non-small cell lung cancer
Xiao-hui WANG1, Ming-tai LI1, Lv-ye GAO1, Yan-long HAO1, Xue-fei FENG1, Yuan-fang ZHAI2, Yan-lin GUO1
Affiliations
  • Department of Pathology, Shanxi Medical University, Taiyuan, Shanxi 030001, China
出版时间: 2024-06-25 doi: 10.20043/j.cnki.MPM.202402040
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摘要
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目的

探讨非小细胞肺癌中蛋白-O-岩藻糖基转移酶1的表达及其临床意义，为非小细胞肺癌的诊断和治疗提供参考依据。

方法

通过TCGA、UALCAN数据库和免疫组化技术探讨POFUT1 mRNA和POFUT1蛋白在非小细胞肺癌的表达情况。提取TCGA数据库的临床数据，采用Kaplan-Meier法进行生存分析，采用COX比例风险回归模型分析POFUT1 mRNA表达水平对非小细胞肺癌患者预后的影响。

结果

POFUT1 mRNA和POFUT1蛋白在肺腺癌和肺鳞癌组织中均高表达（均P<0.05）。肺腺癌中POFUT1 mRNA的表达水平与临床分期相关（P<0.001），肺鳞癌中POFUT1 mRNA的表达与肿瘤直径相关（P<0.001）。COX回归分析结果显示，POFUT1 mRNA高表达（P=0.012）、肿瘤直径、临床分期、淋巴结转移与肺腺癌患者预后相关（均P<0.001），临床III-IV期是肺腺癌患者独立危险因素。肿瘤直径（P=0.001）、临床III-IV期（P=0.006）和POFUT1 mRNA高表达（P=0.049）与肺鳞癌患者预后相关，肿瘤直径大于5cm和POFUT1高表达是肺鳞癌患者独立危险因素。生存分析结果显示POFUT1高表达的非小细胞肺癌患者生存率显著低于低表达患者。

结论

POFUT1在非小细胞肺癌组织中高表达，且其高表达与患者不良预后相关，可作为非小细胞肺癌预后评估的生物标志物。

非小细胞肺癌  /  POFUT1  /  生物信息学  /  免疫组织化学  /  预后
Objective

To investigate the expression and clinical significance of protein-O-fucosyltransferase 1 in non-small cell lung cancer (NSCLC), so as to provide a reference for the diagnosis and treatment of NSCLC.

Methods

The expression of POFUT1in NSCLC was determined by TCGA, UALCAN database and immunohistochemistry. Based on the clinical data form TCGA database were extracted for survival analysis by Kaplan-Meier method, and analysis of the effect of POFUT1 mRNA expression level on prognosis of patients with NSCLC.

Results

POFUT1 was highly expression in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues (all P<0.05). The expression level of POFUT1 mRNA in LUAD was correlated with clinical stage (P<0.001) and that in LUSC was correlated with tumor size (P<0.001). COX regression analysis showed that high mRNA expression of POFUT1 (P=0.012), tumor size, clinical stage and lymph node metastasis were associated with the prognosis of LUAD patients (all P<0.001), and clinical stage III-IV was an independent risk factor for LUAD patients. Tumor size (P=0.001), clinical stage III-IV (P=0.006) and high expression of POFUT1 mRNA (P=0.049) were associated with the prognosis of LUSC patients, and tumor size greater than 5cm and high expression of POFUT1 were independent risk factors for LUSC patients. The survival analysis result showed that the survival rate of patients with high expression of POFUT1 was significantly lower than that of patients with low expression.

Conclusion

POFUT1 is highly expressed in NSCLC tissues, and high POFUT1 expression is correlated with poor prognosis in patients. POFUT1 can serve as a biomarker for prognostic evaluation of NSCLC.

NSCLC  /  POFUT1  /  Bioinformatics  /  Immunohistochemistry  /  Prognosis
王晓慧, 李明泰, 高绿叶, 郝艳龙, 冯雪飞, 翟元芳, 郭艳琳. 蛋白-O-岩藻糖基转移酶1在非小细胞肺癌中的表达及临床意义. 现代预防医学, 2024 , 51 (12) : 2248 -2252 . DOI: 10.20043/j.cnki.MPM.202402040
Xiao-hui WANG, Ming-tai LI, Lv-ye GAO, Yan-long HAO, Xue-fei FENG, Yuan-fang ZHAI, Yan-lin GUO. Expression and clinical significance of protein O-fucosyltransferase 1 in non-small cell lung cancer[J]. Modern Preventive Medicine, 2024 , 51 (12) : 2248 -2252 . DOI: 10.20043/j.cnki.MPM.202402040
正文
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肺癌（lung cancer）是常见的恶性肿瘤之一，在我国和全球范围内其发病率和死亡率均占首位。2022年我国新发肺癌病例106.06万，因肺癌死亡病例73.33万[1]。非小细胞肺癌（non-small cell lung cancer，NSCLC）患者约占肺癌患者总数的85%[2]，其中主要包括肺腺癌（lung adenocarcinoma，LUAD）和肺鳞癌（lung squamous cell carcinoma，LUSC）。临床中NSCLC的诊治虽取得一定进展，但其死亡率仍居高不下。因此，探明NSCLC的分子发病机理，对NSCLC的诊断、治疗和预后均有重要作用。
糖基化是蛋白质的一种翻译后修饰，在细胞生长、分化、黏附和肿瘤免疫监视等过程中发挥作用[3]。据报道，异常的糖基化修饰与恶性肿瘤的发生发展密切相关[4]。岩藻糖基化是糖基化修饰的重要方式，主要依靠岩藻糖化转移酶、岩藻糖化合成酶和岩藻糖转运蛋白的作用将岩藻糖添加到底物中形成寡糖。岩藻糖基转移酶是通过蛋白质的岩藻糖基化作用参与人类癌症分化、发展和恶性化的关键酶[5]。
蛋白-O-岩藻糖基转移酶1（protein O-fucosyltransferase 1，POFUT1）是O-岩藻糖基转移酶的重要成员之一。研究发现，POFUT1在神经胶质瘤、结直肠癌、胃癌等多种实体瘤中高表达，并促进肿瘤细胞的恶性行为[6-8]。但是POFUT1在NSCLC中的表达情况及对患者预后的影响鲜有报道。本研究通过生物信息学分析结合免疫组织化学技术探讨POFUT1在NSCLC中的表达和临床意义，为明确NSCLC诊断和治疗的新靶点提供参考依据。
1　材料与方法
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1.1　数据来源
通过TCGA数据库（https://portal.gdc.cancer.gov/）提取肺腺癌和肺鳞癌RNA-Seq转录组数据和相关临床特征信息。剔除无POFUT1 mRNA表达量和临床特征信息的样本9例。TCGA-LUAD数据集包括58例正常样本，513例LUAD病例；TCGA-LUSC数据集包括51例正常样本，496例LUSC病例。
1.2　生物信息学分析
根据约登指数（敏感度+特异度-1）最大值定义POFUT1 mRNA表达量的最佳临界值。根据TCGA-LUAD中POFUT1 mRNA表达量的最佳临界值4.946 4，将513例LUAD样本分为POFUT1 mRNA高表达组（309例）和POFUT1 mRNA低表达组（204例）；根据TCGA-LUSC中POFUT1 mRNA表达量的最佳临界值6.4301，将496例LUSC样本分为POFUT1 mRNA高表达组（67例）和POFUT1 mRNA低表达组（429例）。利用χ2检验分析POFUT1 mRNA表达量与患者临床特征的关系；应用COX回归分析影响NSCLC患者预后的危险因素。使用R包“ggplot2”绘制POFUT1 mRNA差异表达箱形图，R包“survival”绘制生存曲线。应用UALCAN数据库（http://ualcan.path.uab.edu/analysis-prot.html）CPTAC板块在线分析POFUT1蛋白在NSCLC组织与正常肺组织的差异表达。
1.3　免疫组织化学检测
1.3.1　标本收集
肺组织标本取自山西省肿瘤医院2013年6月至2014年6月呼吸科经组织病理学确诊的NSCLC组织样本128例（71例LUAD和57例LUSC），正常肺组织样本47例。所有病例术前均未接受任何治疗。该研究经山西省肿瘤医院伦理委员会评审通过（伦理编号：201845）。
1.3.2　实验室检测
取4 μm石蜡切片。65℃烤片4 h；用二甲苯去蜡，乙醇脱水处理；高压抗原修复；3%过氧化氢孵育10 min；5%山羊血清37℃封闭30 min；POFUT1一抗（1：200；武汉三鹰公司）4℃过夜孵育；加50 μL二抗，室温孵育1 h；50 μL辣根酶标记链霉卵白素工作液，37℃孵育1 h；DAB呈色；脱水透明处理后，中性树胶封片。
用NanoZoomer-SQ数字切片扫描仪器对组织切片进行扫描，在NDPView 2软件截取5张代表性图片，用ImageJ软件IHC profiler插件分析。根据公式H-score=[1×（低阳性细胞占比）+2×（阳性细胞占比）+3×（高阳性细胞占比）]×100计算每个样本POFUT1蛋白的表达量，分数范围为0到300分[9]。
1.4　统计分析
数据分析使用SPSS 26.0软件完成。计量资料采用均数±标准差表示，独立样本t检验对两组间表达量进行分析。多组样本均数的比较采用单因素方差分析。用χ2检验分析POFUT1的表达与临床特征的关系，其中计数资料用频数（%）表示。检验水准α=0.05。
2　结果
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2.1　POFUT1 mRNA和POFUT1蛋白在LUAD和LUSC中的表达情况
2.1.1　POFUT1 mRNA在LUAD和LUSC中的表达情况
TCGA数据库分析结果显示，POFUT1 mRNA在LUAD（图1A）和LUSC（图1B）中的表达量均显著高于正常肺组织（均P<0.001）。
2.1.2　POFUT1蛋白在LUAD和LUSC中的表达情况
UALCAN数据库分析显示，与正常肺组织相比，POFUT1蛋白在LUAD和LUSC组织中均呈高表达（均P<0.05）（图2A、图2B）。
2.1.3　免疫组化检测POFUT1蛋白在LUAD和LUSC中的表达情况
免疫组化检测结果显示，与正常肺组织相比，POFUT1蛋白在LUAD和LUSC组织中的阳性表达增加（图3A）。对免疫组化结果进行定量分析，POFUT1蛋白在LUAD的表达水平为（100.89±21.67），LUSC为（107.75±31.55），正常肺组织为（71.41±21.62）。单因素方差分析结果显示，POFUT1在LUAD和LUSC中的蛋白表达水平显著高于正常肺组织（F=24.90，P<0.001）（图3B）。
2.2　POFUT1 mRNA表达与LUAD和LUSC患者临床特征的关系
NSCLC患者POFUT1 mRNA表达量与临床特征相关分析结果显示，LUAD患者POFUT1 mRNA的表达与临床分期有关（P<0.001），与性别、肿瘤直径、吸烟史、年龄和淋巴结转移无关（均P>0.05）；LUSC患者POFUT1 mRNA的表达与肿瘤直径相关（P<0.001），与性别、年龄、吸烟史，分期以及淋巴结转移均无关（均P>0.05）（表1）。
2.3　POFUT1 mRNA表达对LUAD和LUSC患者预后的影响
生存分析结果显示，POFUT1 mRNA高表达的LUAD和LUSC患者生存率显著低于POFUT1低表达患者（图4A、图4B），差异有统计学意义（均P<0.05）。单因素COX回归分析结果显示，POFUT1 mRNA高表达（P=0.012）、肿瘤直径、临床分期、淋巴结转移与肺腺癌患者预后相关（均P<0.001）；多因素COX回归结果显示，临床III-IV期肺腺癌患者死亡风险比临床I期患者高1.956倍（95%CI:1.000～3.824，P=0.050）（表2）。单因素回归结果显示，肿瘤直径（P=0.001）、临床III-IV期（P=0.006）和POFUT1 mRNA高表达（P=0.049）与肺鳞癌患者预后相关；多因素结果显示，肿瘤直径大于5cm的肺鳞癌患者死亡风险是肿瘤直径小于5cm患者的1.771倍（95%CI:1.272～2.465，P=0.001），且POFUT1高表达的肺鳞癌患者的死亡风险比POFUT1低表达患者高1.491倍（95%CI:1.041～2.137，P=0.029）（表3）。
3　讨论
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随着基因检测的深入和诊疗技术的提高，肺癌的死亡率逐步下降，但三分之一肺癌患者被确诊时已是中晚期，导致五年生存率偏低，因此肺癌的早期诊断至关重要。目前，蛋白糖基化修饰作为癌症潜在诊断标志物被广泛研究[10]。异常蛋白糖基化修饰与癌症的发展有关[4]。岩藻糖基转移酶的高表达是恶性细胞转化的标志，被认为是实体瘤的潜在治疗靶点[5]。POFUT1作为O-岩藻糖转移酶之一，定位于20q11.21，可通过O-糖苷键将岩藻糖添加到许多细胞表面和分泌蛋白的表皮生长因子样结构域的共识序列中的丝氨酸或苏氨酸残基上，在O-岩藻糖基化中发挥作用[11]。研究发现POFUT1在多种实体瘤组织（如结直肠癌、胃癌等）中的表达水平均显著高于正常组织，提示POFUT1与肿瘤进展相关[6-8]。
Leng团队[12]研究发现与对照组相比，肺癌患者中POFUT1的水平更高，对POFUT1的检测分析有助于肺癌的诊断。本研究的实验结果表明POFUT1 mRNA和蛋白在非小细胞肺癌组织的表达显著高于正常肺组织，提示POFUT1高表达可能与非小细胞肺癌的发生发展有关。研究发现POFUT1高表达与口腔癌组织肿瘤直径正相关[13]，与结直肠癌病理分期密切相关[7]。本研究结果显示，POFUT1高表达与LUAD患者临床分期正相关（P=0.000），与LUSC患者肿瘤直径正相关（P=0.000），提示POFUT1有望作为判定非小细胞肺癌病理分期的一种新的参考指标。
本研究的COX回归结果显示POFUT1高表达和肿瘤直径大于5cm是LUSC患者的独立危险因素；POFUT1高表达和临床分期是影响LUAD患者预后的重要临床指标。多项研究表明POFUT1高表达的肿瘤患者比 POFUT1低表达的患者总生存率更低[6-8]。本研究结果也发现POFUT1表达高的非小细胞肺癌患者的生存期更短（P<0.05）。上述研究结果提示POFUT1可能参与非小细胞肺癌的发生发展，可作为判断患者预后的生物标志物。
本研究通过生物信息学结合免疫组化方法发现POFUT1在非小细胞肺癌中高表达，其高表达与LUAD患者临床分期和LUSC患者肿瘤直径相关，是肺鳞癌患者的独立预后因素，提示POFUT1有助于非小细胞肺癌患者的辅助诊断和预后评估，进一步研究POFUT1为阐明非小细胞肺癌发生发展提供新思路。
基金
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  • 山西省基础研究计划(20210302124185)
  • 山西省回国留学人员科研资助项目(2021-082)
文献
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参考文献 引证文献
排序方式：
[1]
Xia CF, Dong XS, Li H, et al. Cancer statistics in China and United States, 2022: profiles, trends, and determinants[J]. Chinese Medical Journal, 2022, 135(5): 584-590.
[2]
Tan AC, Tan DSW. Targeted therapies for lung cancer patients with oncogenic driver molecular alterations[J]. Journal of Clinical Oncology, 2022, 40(6): 611-625.
[3]
Bangarh R, Khatana C, Kaur S, et al. Aberrant protein glycosylation: Implications on diagnosis and immunotherapy[J]. Biotechnology Advances, 2023, 66: 108149.
[4]
Zhong Q, Xiao XN, Qiu YJ, et al. Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications[J]. MedComm, 2023, 4(3): e261.
[5]
Lv YX, Zhang ZD, Tian S, et al. Therapeutic potential of fucosyltransferases in cancer and recent development of targeted inhibitors[J]. Drug Discovery Today, 2023, 28(1): 103394.
[6]
Li Q, Wang J, Ma XD, et al. POFUT1 acts as a tumor promoter in glioblastoma by enhancing the activation of Notch signaling[J]. Journal of Bioenergetics and Biomembranes, 2021, 53(5): 621-632.
[7]
Long F, Tian BN, Li L, et al. CircPOFUT1 fosters colorectal cancer metastasis and chemoresistance via decoying miR-653-5p/E2F7/WDR66 axis and stabilizing BMI1[J]. iScience, 2024, 27(1): 108729.
[8]
Dong S, Wang ZR, Xiong WJ. POFUT1 promotes gastric cancer progression through Notch/Wnt dual signaling pathways dependent on the parafibromin-NICD1-catenin complex[J]. Journal of the Chinese Medical Association: JCMA, 2023, 86(9): 806-817.
[9]
Varghese F, Bukhari AB, Malhotra R, et al. IHC profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples[J]. PLOS One, 2014, 9(5): e96801.
[10]
Gao GW, Li C, Fan WG, et al. Brilliant glycans and glycosylation: Seq and ye shall find[J]. International Journal of Biological Macromolecules, 2021, 189: 279-291.
[11]
Chabanais J, Labrousse F, Chaunavel A, et al. POFUT1 as a promising novel biomarker of colorectal cancer[J]. Cancers, 2018, 10(11): 411.
[12]
Leng QX, Tsou JH, Zhan M, et al. Fucosylation genes as circulating biomarkers for lung cancer[J]. Journal of Cancer Research and Clinical Oncology, 2018, 144(11): 2109-2115.
[13]
Yokota S,Ogawara K, Kimura R, et al. Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer[J]. International Journal of Oncology, 2013, 43(6): 1864-1870.
2024年第51卷第12期
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文章信息
doi: 10.20043/j.cnki.MPM.202402040
  • 接收时间：2024-02-03
  • 首发时间：2026-03-17
  • 出版时间：2024-06-25
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  • 收稿日期：2024-02-03
基金
山西省基础研究计划(20210302124185)
山西省回国留学人员科研资助项目(2021-082)
作者信息
    1.山西医科大学基础医学院病理教研室，山西 太原 030001
    2.山西医科大学基础医学院解剖教研室

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郭艳琳，E-mail：
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2种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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