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Article(id=1311350176028123871, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202602163, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1770998400000, receivedDateStr=2026-02-14, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790579845672, onlineDateStr=2026-09-28, pubDate=1782316800000, pubDateStr=2026-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790579845672, onlineIssueDateStr=2026-09-28, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790579845672, creator=13701087609, updateTime=1790579845672, updator=13701087609, issue=Issue{id=1311349919793905738, tenantId=1146029695717560320, journalId=1227665162245664772, year='2026', volume='53', issue='12', pageStart='2113', pageEnd='2304', issueExtLink='null', onlineDate='null', pubDate='1782316800000', pubDateStr='2026-06-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790579784582, creator='13701087609', updateTime=1790588408144, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1311386089701597565, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1311386089701597566, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2272, endPage=2277, ext={EN=ArticleExt(id=1311350176841818848, articleId=1311350176028123871, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Functional epitope characterization of the Artemisia vulgaris allergen Art v 4 and a preliminary investigation of its cross-reactive sensitization mechanism with peach Pru p 4, columnId=1228016572065837304, journalTitle=Modern Preventive Medicine, columnName=Experimental Technology and Applications, runingTitle=null, highlight=null, articleAbstract=
Objective

To characterize the functional epitope distribution of the Artemisia vulgaris allergen Art v 4 and to investigate its potential cross-reactive epitopes with the peach allergen Pru p 4.

Methods

Immunoinformatics approaches were used to predict B-cell and T-cell epitopes of Art v 4, followed by spatial localization analysis based on its three-dimensional structure. Potential shared cross-reactive epitopes between Art v 4 and Pru p 4 were screened by sequence alignment. Candidate peptides were synthesized, and their inhibitory effects on the binding of patients’ serum IgE to the natural allergen were evaluated by inhibitory Dot-Blot assay.

Results

Multiple potential linear B-cell epitopes were identified in Art v 4, among which the 13-20 aa and 108-116 aa regions showed high overlap across different prediction algorithms. Among the predicted dominant CD4＋ T-cell epitopes, the 101-115 aa region showed a high tendency for MHC II binding. Art v 4 and Pru p 4 exhibited high sequence homology in multiple regions, and some dominant T-cell epitopes were highly conserved. The 31-45 aa and 101-115 aa peptides significantly inhibited the binding of patients’ serum IgE to the natural allergen.

Conclusion

Art v 4 contains multiple candidate epitopes, and the regions 31-45 aa and 101-115 aa may represent cross-reactive epitope regions worthy of further investigation.

, authors=Ruo-lin SONG, Hui-lian CHE, authorsList=Ruo-lin SONG, Hui-lian CHE, authorCompany=null, correspAuthors=Hui-lian CHE, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1311350185326895853, articleId=1311350176028123871, tenantId=1146029695717560320, journalId=1227665162245664772, language=CN, title=艾蒿过敏原Art v 4功能表位及其与桃Pru p 4交叉致敏机制初探, columnId=1228016572288135432, journalTitle=现代预防医学, columnName=实验技术及其应用, runingTitle=null, highlight=null, articleAbstract=
目的

解析艾蒿过敏原Art v 4的功能表位分布特征，并探讨其与桃过敏原Pru p 4之间潜在交叉表位。

方法

通过免疫信息学方法预测Art v 4 B细胞表位及T细胞表位，并结合三维结构进行定位分析。通过序列比对筛选与Pru p 4共享的潜在交叉表位。合成候选肽段后，以抑制性Dot-Blot检测其与患者血清IgE的结合抑制作用。

结果

Art v 4蛋白中存在多个潜在线性B细胞表位，其中13~20 aa与108~116 aa区段在多种预测算法中重叠较高。预测获得的潜在优势CD4＋ T细胞表位中，101~115 aa表现出较高的MHC II结合倾向。Art v 4与Pru p 4在多个区段存在较高同源性，部分优势T细胞表位高度保守。31~45 aa与101~115 aa肽段可显著抑制患者血清IgE与天然过敏原结合。

结论

Art v 4具有多个候选表位，31~45 aa、101~115 aa区段可能是值得进一步关注的交叉表位区域。

, authors=宋若琳, 车会莲, authorsList=宋若琳, 车会莲, authorCompany=null, correspAuthors=车会莲, authorNote=

宋若琳（2001—），女，硕士在读，研究方向：食品安全及过敏机制研究

, correspAuthorsNote=
车会莲，E-mail:
, copyrightStatement=本刊刊出的所有文章不代表中华预防医学会和本刊编委会的观点，除非特别声明。, copyrightOwner=中华预防医学会和四川大学华西公共卫生学院, extLink=null, articleAbsUrl=null, sourceXml=u9+pz0PRJZ2TRRZqBldAXA==, magXml=tS09e8uMbfnIfR3FDBSRJw==, pdfUrl=null, pdf=qfi1u8Md7IUiVeorIF/ddA==, pdfFileSize=962350, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=y6abSALFjDAkJwZkHuHNgw==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=/49AvqHweyId06DA70iYbA==, mapNumber=null, fund=null)}, authors=[Author(id=1311350188615230194, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1311350189852549876, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, authorId=1311350188615230194, language=EN, stringName=Ruo-lin SONG, firstName=Ruo-lin, middleName=null, lastName=SONG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1311350190196482805, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, authorId=1311350188615230194, language=CN, stringName=宋若琳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国农业大学食品科学与营养工程学院，北京 100083, bio={"content":"

宋若琳（2001—），女，硕士在读，研究方向：食品安全及过敏机制研究

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Commun Biol, 2022, 5(1): 748., articleTitle=A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity, refAbstract=null), Reference(id=1311350231313244977, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, doi=null, pmid=null, pmcid=null, year=2023, volume=151, issue=5, pageStart=1178, pageEnd=1190, url=null, language=null, rfNumber=[13], rfOrder=14, authorNames=Kamath S, Bublin M, Kitamura K, journalName=Journal of Allergy and Clinical Immunology, refType=null, unstructuredReference=Kamath S, Bublin M, Kitamura K, et al. Cross-reactive epitopes and their role in food allergy ［J］. Journal of Allergy and Clinical Immunology, 2023, 151(5): 1178-1190., articleTitle=Cross-reactive epitopes and their role in food allergy, refAbstract=null), Reference(id=1311350231401325363, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, doi=null, pmid=null, pmcid=null, year=2025, volume=17, issue=1, pageStart=147, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=15, authorNames=Álvaro-benito M, Abualrous E, Lingel H, journalName=Genome Medicine, refType=null, unstructuredReference=Álvaro-benito M, Abualrous E, Lingel H, et al. Cut or bind? Antigen-specific processing mechanisms define CD4+ T cell immunodominant epitopes for SARS-CoV-2 S and N proteins ［J］. Genome Medicine, 2025, 17(1): 147., articleTitle=Cut or bind? 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Front Med, 2024, 11: 1388766., articleTitle=Profiles of apple allergen components and its diagnostic value in Northern China, refAbstract=null), Reference(id=1311350234303783735, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, doi=null, pmid=null, pmcid=null, year=2025, volume=11, issue=1, pageStart=3, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=18, authorNames=Liu S, Chu J, Yin X, journalName=NPJ Vaccines, refType=null, unstructuredReference=Liu S, Chu J, Yin X, et al. The adeno-associated viral vectored Dp12S vaccine effective alleviation of asthma symptoms in mice［J］. NPJ Vaccines, 2025, 11(1): 3., articleTitle=The adeno-associated viral vectored Dp12S vaccine effective alleviation of asthma symptoms in mice, refAbstract=null), Reference(id=1311350234698048312, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, doi=null, pmid=null, pmcid=null, year=2024, volume=13, issue=21, pageStart=3440, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=19, authorNames=Shwe YN, Ng HK, Zeng J, journalName=Foods, refType=null, unstructuredReference=Shwe YN, Ng HK, Zeng J, et al. Development of cashew and pistachio ladders through a food-processing approach［J］. Foods, 2024, 13(21): 3440., articleTitle=Development of cashew and pistachio ladders through a food-processing approach, refAbstract=null), Reference(id=1311350235239113529, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, doi=null, pmid=null, pmcid=null, year=2024, volume=24, issue=9, pageStart=471, pageEnd=475, url=null, language=null, rfNumber=[19], rfOrder=20, authorNames=Rabin RL, journalName=Current Allergy and Asthma Reports, refType=null, unstructuredReference=Rabin RL. The potential of human monoclonal IgE antibodies to establish biological potency and stability of allergen extract［J］. Current Allergy and Asthma Reports, 2024, 24(9): 471-475., articleTitle=The potential of human monoclonal IgE antibodies to establish biological potency and stability of allergen extract, refAbstract=null)], funds=[Fund(id=1311350221246915361, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, awardId=32541102, language=CN, fundingSource=国家自然科学基金专项项目(32541102), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1311350187361133294, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, xref=null, ext=[AuthorCompanyExt(id=1311350187369521903, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, companyId=1311350187361133294, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China), AuthorCompanyExt(id=1311350187377910512, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, companyId=1311350187361133294, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国农业大学食品科学与营养工程学院，北京 100083)])], figs=[ArticleFig(id=1311350201164587783, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Fig.1, caption=DNAstrar protean analysis of the amino acid sequence of Art v 4, figureFileSmall=heUYJ5XtVcKaHjeUGmyz2w==, figureFileBig=y6abSALFjDAkJwZkHuHNgw==, tableContent=null), ArticleFig(id=1311350202053780232, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=图1, caption=Art v 4的氨基酸序列的DNAstar Protean分析

注：Hydrophilicity plot：亲水区域；Flexible Regions：柔性区域；Antigen Index：抗原指数；Surface Probability：表面可及性。

, figureFileSmall=heUYJ5XtVcKaHjeUGmyz2w==, figureFileBig=y6abSALFjDAkJwZkHuHNgw==, tableContent=null), ArticleFig(id=1311350202473210633, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Fig.2, caption=Sequence homology analysis of Art v 4 and Pru p 4, figureFileSmall=vm3FpvUeGYqBYb8T0kVyAA==, figureFileBig=z9ZOWQCNA7U88vDYyDVkJg==, tableContent=null), ArticleFig(id=1311350203232379658, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=图2, caption=Art v 4与Pru p 4的序列同源性分析

注：若某一列残基完全相同，标记为“*”；若该列残基虽不完全相同，但分子大小和亲疏水性相似（无明显不相似替换），标记为“：”；若该列仅部分保留理化性质，存在相似与不相似残基混合替换，标记为“.”。

, figureFileSmall=vm3FpvUeGYqBYb8T0kVyAA==, figureFileBig=z9ZOWQCNA7U88vDYyDVkJg==, tableContent=null), ArticleFig(id=1311350203693753099, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Fig.3, caption=Three-dimensional structural model of Art v 4 and the cross-reactive CD4+/CD8+ T-cell epitope regions, figureFileSmall=PfUQSerpD2HpHgy/UFanlQ==, figureFileBig=TIBnNgXX/RmvTh6Z6ScCKw==, tableContent=null), ArticleFig(id=1311350204100600588, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=图3, caption=Art v 4三维结构模型及交叉CD4＋/CD8＋ T细胞表位区段

注：深色为A31、A71、A101三个肽段。

, figureFileSmall=PfUQSerpD2HpHgy/UFanlQ==, figureFileBig=TIBnNgXX/RmvTh6Z6ScCKw==, tableContent=null), ArticleFig(id=1311350204549391117, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Fig.4, caption=IDot-Blot results and densitometric analysis of competitive binding of peach extract and Artemisia vulgaris allergen to specific IgE, figureFileSmall=XdaAzWaZACZsH6rZCsh6Kg==, figureFileBig=hNgiYY7YUWZZ0phovFWapQ==, tableContent=null), ArticleFig(id=1311350206596211470, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=图4, caption=桃提取物与艾蒿过敏原竞争性结合特异性IgE的iDot-Blot的结果及灰度值分析

注：上方为代表性iDot-Blot结果图，下方为灰度值定量分析结果。Y-B：健康对照血清与PBS预孵育组；A-B：艾蒿过敏患者混合血清与PBS预孵育组；A-M：艾蒿过敏患者混合血清与艾蒿提取物预孵育组；A-P：艾蒿过敏患者混合血清与桃提取物预孵育组。以膜上点加天然艾蒿提取物作为固相抗原。结果以均值±标准差表示。不同小写字母表示组间差异具有统计学意义（α=0.05），相同字母表示差异无统计学意义。

, figureFileSmall=XdaAzWaZACZsH6rZCsh6Kg==, figureFileBig=hNgiYY7YUWZZ0phovFWapQ==, tableContent=null), ArticleFig(id=1311350207003058959, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Fig.5, caption=IDot-Blot results and densitometric analysis of competitive binding of synthetic peptides and Artemisia vulgaris allergen to specific IgE, figureFileSmall=/RhSSyiZhW9iRuVjzo3LpQ==, figureFileBig=4c8qiXkLT+oGmwlge2yiCw==, tableContent=null), ArticleFig(id=1311350207892251408, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=图5, caption=合成多肽与艾蒿过敏原竞争性结合特异性IgE的iDot-Blot的结果及灰度值分析

注：Y-B：健康对照血清与PBS预孵育组；A-B：艾蒿过敏患者混合血清与PBS缓冲液预孵育组；A-M：艾蒿过敏患者混合血清与艾蒿提取物预孵育组；A-31：艾蒿过敏患者混合血清与A31肽段预孵育组；A-101：艾蒿过敏患者混合血清与A101肽段预孵育组。以膜上点加天然艾蒿提取物作为固相抗原。

, figureFileSmall=/RhSSyiZhW9iRuVjzo3LpQ==, figureFileBig=4c8qiXkLT+oGmwlge2yiCw==, tableContent=null), ArticleFig(id=1311350208408150801, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 1, caption=

Information of patients and healthy controls

, figureFileSmall=null, figureFileBig=null, tableContent=
序号性别年龄过敏原及等级
1男41~50狗毛皮屑（+++）；艾蒿（++++）
2女31~40牛奶（++）；粉尘螨（+++）；艾蒿（++++）
3男19~30艾蒿（++++）
4男19~30虾（++++）、尘螨（++++）、艾蒿（++++）
5女19~30无
6女41~50无
), ArticleFig(id=1311350208643031826, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表1, caption=

过敏患者及健康对照者信息

, figureFileSmall=null, figureFileBig=null, tableContent=
序号性别年龄过敏原及等级
1男41~50狗毛皮屑（+++）；艾蒿（++++）
2女31~40牛奶（++）；粉尘螨（+++）；艾蒿（++++）
3男19~30艾蒿（++++）
4男19~30虾（++++）、尘螨（++++）、艾蒿（++++）
5女19~30无
6女41~50无
), ArticleFig(id=1311350209486086931, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 2, caption=

Linear B-cell epitopes of Art v 4 predicted by two immunoinformatics tools

, figureFileSmall=null, figureFileBig=null, tableContent=
预测软件预测结果
ABCpred（https://webs.iiitd.edu.in/raghava/abcpred/）（threshold=0.50）4~19，12~27，19~34，26~41，33~48，40~55，50~65，57~72，70~85，81~96，91~106，99~114，105~120，113~128
BepiPred-3.0（https://services.healthtech.dtu.dk/service.php?BepiPred-3.0）（threshold=0.15）13~20，108~116
), ArticleFig(id=1311350211172197140, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表2, caption=

两种免疫信息学工具预测的Art v 4线性B细胞表位

, figureFileSmall=null, figureFileBig=null, tableContent=
预测软件预测结果
ABCpred（https://webs.iiitd.edu.in/raghava/abcpred/）（threshold=0.50）4~19，12~27，19~34，26~41，33~48，40~55，50~65，57~72，70~85，81~96，91~106，99~114，105~120，113~128
BepiPred-3.0（https://services.healthtech.dtu.dk/service.php?BepiPred-3.0）（threshold=0.15）13~20，108~116
), ArticleFig(id=1311350211625181973, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 3, caption=

Predicted conformational B-cell epitopes of Art v 4

, figureFileSmall=null, figureFileBig=null, tableContent=
序号表位残基范围残基数ElliPro评分
1S1，G0，S2，W3，Q4，T5，D8，D980.746
2T99，G100，Q101，D126，Y127，L129，D130，Q131，G132，M133100.723
3M12~L22区段110.701
4P42~A113多区域结构表位320.695
5Q78~A83区段60.566
6T30~T33区段40.554
7S38~S40区段30.517
), ArticleFig(id=1311350212011057942, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表3, caption=

Art v 4构象B细胞表位预测结果

, figureFileSmall=null, figureFileBig=null, tableContent=
序号表位残基范围残基数ElliPro评分
1S1，G0，S2，W3，Q4，T5，D8，D980.746
2T99，G100，Q101，D126，Y127，L129，D130，Q131，G132，M133100.723
3M12~L22区段110.701
4P42~A113多区域结构表位320.695
5Q78~A83区段60.566
6T30~T33区段40.554
7S38~S40区段30.517
), ArticleFig(id=1311350212426294039, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 4, caption=

Potential dominant CD4+ T-cell epitopes of Art v 4 predicted by NetMHCIIpan

, figureFileSmall=null, figureFileBig=null, tableContent=
序号起止位点氨基酸序列最佳HLA等位基因百分位值（%）核心序列
101~115QAMVFGIYDEPVAPGHLA-DQA101:01/DQB105:010.05VFGIYDEPV
271~85GAKYMVIQGEAGAVIHLA-DRB1*01:010.32YMVIQGEAG
331~45DGTVWAKSASFPEFKHLA-DRB1*09:010.34WAKSASFPE
441~55FPEFKPNEIDAIIKEHLA-DRB1*09:010.37PNEIDAIIK
566~80GLFLGGAKYMVIQGEHLA-DRB1*01:012.10GAKYMVIQG
61~15MSWQTYVDDHLMCDIHLA-DRB1*01:012.12TYVDDHLM
746~60PNEIDAIIKEFNEAGHLA-DRB1*09:012.18DAIIKEFNE
891~105AGGICIKKTGQAMVFHLA-DRB1*01:012.30TGQAMVFGI
), ArticleFig(id=1311350212879278872, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表4, caption=

NetMHCIIpan预测的Art v 4潜在优势CD4＋ T细胞表位

, figureFileSmall=null, figureFileBig=null, tableContent=
序号起止位点氨基酸序列最佳HLA等位基因百分位值（%）核心序列
101~115QAMVFGIYDEPVAPGHLA-DQA101:01/DQB105:010.05VFGIYDEPV
271~85GAKYMVIQGEAGAVIHLA-DRB1*01:010.32YMVIQGEAG
331~45DGTVWAKSASFPEFKHLA-DRB1*09:010.34WAKSASFPE
441~55FPEFKPNEIDAIIKEHLA-DRB1*09:010.37PNEIDAIIK
566~80GLFLGGAKYMVIQGEHLA-DRB1*01:012.10GAKYMVIQG
61~15MSWQTYVDDHLMCDIHLA-DRB1*01:012.12TYVDDHLM
746~60PNEIDAIIKEFNEAGHLA-DRB1*09:012.18DAIIKEFNE
891~105AGGICIKKTGQAMVFHLA-DRB1*01:012.30TGQAMVFGI
), ArticleFig(id=1311350213697168153, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 5, caption=

Potential dominant CD8+ T-cell epitopes of Art v 4 predicted by NetMHCpan

, figureFileSmall=null, figureFileBig=null, tableContent=
排名位置序列HLA-I等位基因百分位值（%）
111~19HLMCDIAAMHLA-A*02:010.20
287~95RRGGICIKKHLA-A*30:010.21
388~96RGGICIKKTHLA-A*30:010.21
490~98GICIKKTGQHLA-A*02:010.21
574~82VIQGEAGAVHLA-A*02:010.38
630~38GDGTVWAKSHLA-A*02:011.54
731~39DGTVWAKSAHLA-A*02:011.63
856~64PNEIDAIIKHLA-A*30:011.18
976~84IQGEAGAVIHLA-A*02:010.87
1077~85QGEAGAVIRHLA-A*30:011.41
), ArticleFig(id=1311350214506668826, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表5, caption=

NetMHCpan预测的Art v 4潜在优势CD8＋ T细胞表位

, figureFileSmall=null, figureFileBig=null, tableContent=
排名位置序列HLA-I等位基因百分位值（%）
111~19HLMCDIAAMHLA-A*02:010.20
287~95RRGGICIKKHLA-A*30:010.21
388~96RGGICIKKTHLA-A*30:010.21
490~98GICIKKTGQHLA-A*02:010.21
574~82VIQGEAGAVHLA-A*02:010.38
630~38GDGTVWAKSHLA-A*02:011.54
731~39DGTVWAKSAHLA-A*02:011.63
856~64PNEIDAIIKHLA-A*30:011.18
976~84IQGEAGAVIHLA-A*02:010.87
1077~85QGEAGAVIRHLA-A*30:011.41
), ArticleFig(id=1311350215823680283, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 6, caption=

BLAST alignment results of the amino acid sequences of Art v 4 and Pru p 4

, figureFileSmall=null, figureFileBig=null, tableContent=
比对序列覆盖率（%）E值一致性（%）序列长度（aa）
Art v 4 vs. Pru p 41002.00×10-7772.93133
), ArticleFig(id=1311350216280859420, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表6, caption=

Art v 4与Pru p 4氨基酸序列BLAST比对结果

, figureFileSmall=null, figureFileBig=null, tableContent=
比对序列覆盖率（%）E值一致性（%）序列长度（aa）
Art v 4 vs. Pru p 41002.00×10-7772.93133
), ArticleFig(id=1311350217115525917, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=EN, label=Table 7, caption=

Cross-reactive CD4＋/CD8＋ T-Cell epitopes of Art v 4 and Pru p 4

, figureFileSmall=null, figureFileBig=null, tableContent=
序号Art v 4起止Art v 4表位核心序列百分位值（%）Pru p 4起止Pru p 4表位相似度交叉潜力
171~85GAKYMVIQGEAGAVIYMVIQGEAG0.3271~85KYMVIQGEAGAVIRG1.00高
266~80GLFLGGAKYMVIQGEGAKYMVIQG2.1071~85KYMVIQGEAGAVIRG~0.83高
31~15MSWQTYVDDHLMCDITYVDDHLM2.121~15MSWQAYVDDHLMCDI~0.87高
431~45DGTVWAKSASFPEFKWAKSASFPE0.3431~45SVWSQSATFPAFKPE~0.62中
5101~115QAMVFGIYDEPVAPGVFGIYDEPV0.0596~110/101~115KTNQALIIGIYDEPL/LIIGIYDEPLTPGQC~0.67中
691~105AGGICIKKTGQAMVFTGQAMVFGI2.3096~110KTNQALIIGIYDEPL~0.40低
), ArticleFig(id=1311350217908249374, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311350176028123871, language=CN, label=表7, caption=

Art v 4和Pru p 4的交叉CD4＋/CD8＋ T细胞表位

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序号Art v 4起止Art v 4表位核心序列百分位值（%）Pru p 4起止Pru p 4表位相似度交叉潜力
171~85GAKYMVIQGEAGAVIYMVIQGEAG0.3271~85KYMVIQGEAGAVIRG1.00高
266~80GLFLGGAKYMVIQGEGAKYMVIQG2.1071~85KYMVIQGEAGAVIRG~0.83高
31~15MSWQTYVDDHLMCDITYVDDHLM2.121~15MSWQAYVDDHLMCDI~0.87高
431~45DGTVWAKSASFPEFKWAKSASFPE0.3431~45SVWSQSATFPAFKPE~0.62中
5101~115QAMVFGIYDEPVAPGVFGIYDEPV0.0596~110/101~115KTNQALIIGIYDEPL/LIIGIYDEPLTPGQC~0.67中
691~105AGGICIKKTGQAMVFTGQAMVFGI2.3096~110KTNQALIIGIYDEPL~0.40低
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Quality identification results of epitope peptides A31 and A101

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肽段理论分子量（Da）实测分子量（Da）纯度（%）主要保留时间（min）
A311 669.861 669.898.713.992
A711 506.781 506.675.413.333
A1011 593.821 593.698.512.081
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表位肽A31与A101质量检测结果

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肽段理论分子量（Da）实测分子量（Da）纯度（%）主要保留时间（min）
A311 669.861 669.898.713.992
A711 506.781 506.675.413.333
A1011 593.821 593.698.512.081
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艾蒿过敏原Art v 4功能表位及其与桃Pru p 4交叉致敏机制初探
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宋若琳 , 车会莲
现代预防医学 | 实验技术及其应用 2026,53(12): 2272-2277
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现代预防医学 |实验技术及其应用 2026 , 53 (12) : 2272 -2277
艾蒿过敏原Art v 4功能表位及其与桃Pru p 4交叉致敏机制初探
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宋若琳（2001—），女，硕士在读，研究方向：食品安全及过敏机制研究

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宋若琳, 车会莲
作者信息
  • 中国农业大学食品科学与营养工程学院，北京 100083
通讯作者:
车会莲，E-mail:
作者简介:

宋若琳（2001—），女，硕士在读，研究方向：食品安全及过敏机制研究

Functional epitope characterization of the Artemisia vulgaris allergen Art v 4 and a preliminary investigation of its cross-reactive sensitization mechanism with peach Pru p 4
Ruo-lin SONG, Hui-lian CHE
Affiliations
  • College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
出版时间: 2026-06-25 doi: 10.20043/j.cnki.MPM.202602163
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摘要
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目的

解析艾蒿过敏原Art v 4的功能表位分布特征，并探讨其与桃过敏原Pru p 4之间潜在交叉表位。

方法

通过免疫信息学方法预测Art v 4 B细胞表位及T细胞表位，并结合三维结构进行定位分析。通过序列比对筛选与Pru p 4共享的潜在交叉表位。合成候选肽段后，以抑制性Dot-Blot检测其与患者血清IgE的结合抑制作用。

结果

Art v 4蛋白中存在多个潜在线性B细胞表位，其中13~20 aa与108~116 aa区段在多种预测算法中重叠较高。预测获得的潜在优势CD4＋ T细胞表位中，101~115 aa表现出较高的MHC II结合倾向。Art v 4与Pru p 4在多个区段存在较高同源性，部分优势T细胞表位高度保守。31~45 aa与101~115 aa肽段可显著抑制患者血清IgE与天然过敏原结合。

结论

Art v 4具有多个候选表位，31~45 aa、101~115 aa区段可能是值得进一步关注的交叉表位区域。

Art v 4  /  Profilin  /  交叉反应  /  T细胞表位  /  免疫信息学
Objective

To characterize the functional epitope distribution of the Artemisia vulgaris allergen Art v 4 and to investigate its potential cross-reactive epitopes with the peach allergen Pru p 4.

Methods

Immunoinformatics approaches were used to predict B-cell and T-cell epitopes of Art v 4, followed by spatial localization analysis based on its three-dimensional structure. Potential shared cross-reactive epitopes between Art v 4 and Pru p 4 were screened by sequence alignment. Candidate peptides were synthesized, and their inhibitory effects on the binding of patients’ serum IgE to the natural allergen were evaluated by inhibitory Dot-Blot assay.

Results

Multiple potential linear B-cell epitopes were identified in Art v 4, among which the 13-20 aa and 108-116 aa regions showed high overlap across different prediction algorithms. Among the predicted dominant CD4＋ T-cell epitopes, the 101-115 aa region showed a high tendency for MHC II binding. Art v 4 and Pru p 4 exhibited high sequence homology in multiple regions, and some dominant T-cell epitopes were highly conserved. The 31-45 aa and 101-115 aa peptides significantly inhibited the binding of patients’ serum IgE to the natural allergen.

Conclusion

Art v 4 contains multiple candidate epitopes, and the regions 31-45 aa and 101-115 aa may represent cross-reactive epitope regions worthy of further investigation.

Art v 4  /  Profilin  /  Cross-reactivity  /  T-cell epitope  /  Immunoinformatics
宋若琳, 车会莲. 艾蒿过敏原Art v 4功能表位及其与桃Pru p 4交叉致敏机制初探. 现代预防医学, 2026 , 53 (12) : 2272 -2277 . DOI: 10.20043/j.cnki.MPM.202602163
Ruo-lin SONG, Hui-lian CHE. Functional epitope characterization of the Artemisia vulgaris allergen Art v 4 and a preliminary investigation of its cross-reactive sensitization mechanism with peach Pru p 4[J]. Modern Preventive Medicine, 2026 , 53 (12) : 2272 -2277 . DOI: 10.20043/j.cnki.MPM.202602163
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艾蒿（Artemisia vulgaris）花粉是我国重要的吸入性过敏原之一，是夏秋季季节性过敏性疾病的主要诱因［1-3］。近年来，花粉-食物过敏综合征（pollen-food allergy syndrome, PFAS）受到越来越多关注，主要由花粉过敏原与植物性食物中过敏原之间的交叉反应引起。目前，肌动蛋白结合蛋白（profilin）、病原相关蛋白10（pathogenesis-related protein 10, PR-10）和脂质转移蛋白（lipid transfer protein, LTP）被认为是是介导花粉-食物交叉反应的重要过敏原家族。
艾蒿花粉含有多种分子过敏原，Art v 4属于profilin家族，虽然并非蒿属花粉中最主要的致敏组分，但由于其具有较高的序列和结构保守性，常被认为参与到花粉与多种植物性食物之间的广泛交叉识别［4］。既往文献已将Art v 4与桃过敏原Pru p 4作为艾蒿-桃关联中可能涉及的profilin通路之一加以报道［5-6］。然而，目前针对Art v 4优势表位、其与Pru p 4的共享表位及分子基础研究仍相对有限。
基于此，本研究以Art v 4为对象，系统预测并筛选优势功能区域，与Pru p 4进行同源性比对，筛选潜在交叉表位，并通过体外实验进行初步验证，以期为艾蒿-桃相关profilin通路的机制研究提供依据。
1　材料与方法
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1.1　艾蒿过敏原Art v 4及桃过敏原Pru p 4序列的获取
从NCBI数据库获取Art v 4及Pru p 4氨基酸序列的FASTA格式文件。采用ExPASy ProtParam在线工具其分子量、理论等电点及不稳定指数等理化性质进行预测。
1.2　线性与构象B细胞表位的预测与筛选
在线性B细胞表位预测中，先采用ABCpred对候选区段进行初筛；再采用BepiPred-3.0进行进一步筛选，保留重叠的候选区段；结合DNASTAR Protean综合分析蛋白柔性、表面可及性等，筛选潜在优势B细胞表位区域。构象B细胞表位通过ElliPro进行预测。
1.3　T细胞表位的预测与筛选
采用IEDB数据库中的NetMHCIIpan及NetMHCpan工具，分别用于CD4＋和CD8＋ T细胞表位筛选。选取常见HLA等位基因进行预测，以percentile值≤10%或rank值≤2%作为候选筛选标准。
1.4　Art v 4与Pru p 4的同源序列比对与潜在交叉表位空间定位
采用Clustal Omega进行序列比对，分析同源性及保守区段分布情况。Art v 4三维结构基于AlphaFold数据库模型进行评价，对候选表位在空间结构中的位置进行定位，分析其表面暴露特征及结构分布。
1.5　艾蒿过敏患者血清与桃提取物竞争抑制实验
采用抑制性斑点免疫印迹（inhibitory Dot-Blot，iDot-Blot）评价桃提取物在艾蒿过敏患者血清水平的竞争抑制作用。过敏患者及健康对照者血清样本的采集和使用经中国农业大学人类研究伦理委员会批准（批准号：CAUHR-20230606），所有受试者均签署知情同意书。受试者通过校内招募及社交平台问卷招募的方式征集，并结合临床病史、过敏原检测结果及问卷信息进行筛选，最终纳入艾蒿过敏患者及健康对照者，其中健康对照者均无明确过敏病史且过敏原检测结果均为阴性。采集静脉血后分离血清，分装并于-80 ℃保存备用，患者基本信息见表1。
将4例艾蒿过敏患者血清等体积混合作为混合血清，以PBS稀释后备用。分别以桃提取物、艾蒿提取物与血清预孵育，其中桃提取物组为交叉抑制组，艾蒿提取物组为阳性抑制对照组，PBS组为空白对照组；健康对照血清经相同处理后作为阴性对照。
以艾蒿提取物为固相抗原进行iDot-Blot检测。NC膜封闭后分别加入各组预孵育血清孵育，洗膜后加入HRP标记抗人IgE抗体，采用化学发光法显色并成像，比较各组显色强度以评价桃提取物的竞争抑制作用。
1.6　表位肽的合成、质量鉴定与IgE抑制性验证
根据筛选结果选取3段潜在交叉T细胞表位肽，即31~45 aa、71~85 aa和101~115 aa，分别命名为A31、A71和A101，由南京金斯瑞生物科技股份有限公司采用Fmoc固相合成法合成。采用高效液相色谱（HPLC）检测合成肽纯度，选择纯度大于95%的肽段进行后续研究。合成肽的分子质量通过电喷雾电离-质谱（ESI-MS）确认。合成多肽于-20 ℃保存备用。采用iDot-Blot方法评价表位肽与艾蒿过敏患者血清IgE的结合能力。
1.7　图像灰度值分析与统计学处理
采用ImageJ软件对化学发光成像所得Dot-Blot图像进行灰度值分析，并以PBS预孵育组归一化为100%，得到相对IgE结合强度。所有实验均独立重复至少3次，结果以表示。采用GraphPad Prism软件10.0进行统计学分析，两组间比较采用独立样本t检验，多组间比较采用单因素方差分析。以α=0.05为检验水准，P<0.05表示差异有统计学意义。
2　结果
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2.1　Art v 4表位分析
ExPASy ProtParam分析结果显示，Art v 4由133个氨基酸组成，预测分子量为14.21 kDa，符合profilin家族小分子蛋白的序列特征。GRAVY值为-0.002，提示Art v 4整体呈亲水性特征。结合DNAstar Protean分析结果，Art v 4在42~48 aa，85~102 aa，108~116 aa表现出良好的亲水性、柔性及抗原性特征，可能为潜在表位分布区域，如图1所示。
线性B细胞表位预测结果显示，ABCpred预测共筛选获得14个潜在线性B细胞表位区段。进一步地，BepiPred-3.0预测获得2个评分较高的候选表位区段，位于13~20 aa和108~116 aa，如表2所示。构象B细胞表位预测共获得7组候选表位，评分较高的表位主要分布于蛋白N端和C端，如表3所示。
T细胞表位预测结果显示，Art v 4可筛得若干潜在CD4＋和CD8＋ T细胞表位，其中NetMHCIIpan 4.1预测获得8条潜在优势CD4＋ T细胞表位，如表4；NetMHCpan 4.1预测获得10条潜在优势CD8＋ T细胞表位，如表5。
综合线性B细胞表位、构象B细胞表位、CD4＋/CD8＋ T细胞表位，优先保留在多种预测结果中重叠的候选区段，得到11~20 aa，31~45 aa，71~85 aa，87~98 aa，101~115 aa 5条潜在表位。
2.2　Art v 4与Pru p 4交叉表位分析
为探讨桃过敏原Pru p 4与艾蒿过敏原Art v 4之间潜在的交叉反应基础，采用BLAST对两者氨基酸序列进行整体比对。结果显示，Art v 4与Pru p 4的序列覆盖率为100%，一致性为72.93%，具有较高同源性，如表6所示。进一步采用Clustal Omega精细比对结果显示，两者在1~15 aa，31~94 aa，101~133 aa存在连续高度保守区段，如图2所示，与前述B细胞及T细胞表位预测结果具有明显重叠，提示这些区域可能构成Pru p 4与Art v 4交叉反应的潜在结构基础。
比对Art v 4和Pru p 4的交叉CD4＋/CD8＋ T细胞表位如表7所示，综合序列保守性及表位预测结果，筛选得到Art v 4的31~45 aa、71~85 aa和101~115 aa作为潜在核心交叉表位区段。三维结构分析结果显示，上述区段均位于蛋白表面，主要分布于环区及短α-螺旋区域，具有较好的空间可及性，符合表位识别与抗原呈递的结构特征，如图3所示。
进一步采用抑制性iDot-Blot评价桃提取物对艾蒿过敏患者血清IgE识别艾蒿提取物的竞争抑制作用。结果显示，与空白对照组相比，桃提取物预孵育后患者血清IgE与膜上艾蒿提取物的结合信号明显减弱，且相对IgE结合强度显著降低（α=0.05）；其抑制效果与艾蒿提取物阳性抑制组差异不显著，如图4所示。上述结果表明，桃提取物可与艾蒿过敏患者血清中的部分特异性IgE发生竞争结合，提示Pru p 4与Art v 4之间可能存在交叉反应。
2.3　表位肽质量鉴定及iDot-Blot实验
为评价合成表位肽的质量，采用高效液相色谱和质谱对候选肽段进行检测。结果显示，A31和A101的实测分子量与理论值基本一致，纯度均大于98%，符合后续免疫学功能实验要求；A71纯度为75.4%，未纳入后续IgE抑制实验，仅用于候选区段结构分析参考，如表8所示。
进一步采用iDot-Blot方法评价候选表位肽对艾蒿过敏患者血清IgE与天然艾蒿过敏原结合的抑制作用。结果如图5所示，PBS预孵育组显色明显，而A31和A101预孵育后显色均显著减弱，其抑制效果与阳性对照组差异不显著，表明A31和A101均可与患者血清IgE发生竞争结合，并有效抑制其与天然过敏原的结合，具有较强的IgE抑制能力。
3　讨论
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本研究综合免疫信息学预测、序列比对、结构定位及血清学实验等多方面手段，对Art v 4的候选功能表位及其与桃来源过敏原潜在的共享识别基础展开初步探究。
在线性B细胞表位筛选中，本研究综合运用了ABCpred与BepiPred-3.0。这两种工具算法原理和输出方式不同，ABCpred基于神经网络对固定长度肽段进行预测，更适合作为候选区段的初筛工具，因此通常给出较多表位区段；BepiPred-3.0则基于蛋白语言模型对单个氨基酸残基进行表位倾向评分，并依据连续高分残基形成候选区段，因此结果相对更保守。目前，使用ABCpred初步筛选、BepiPred-3.0进一步收敛，并结合理化性质综合判断，已被表位研究所广泛选择［7-9］。
在B细胞表位层面，本研究结合免疫信息学预测和抑制性iDot-Blot实验，提示13~20 aa，108~116 aa区段可能是Art v 4上较重要的线性IgE识别区域。这一结果与既往关于profilin的表面暴露区域更易形成抗体结合位点的认识基本一致［10-11］，与既往关于profilin蛋白的N端及C端区域常包含较重要的抗体识别位点的报道也相符［12-13］。
在T细胞表位层面，本研究筛得若干候选CD4＋ T细胞表位，其中101~115 aa区段在预测中表现出较高的MHC II结合倾向，提示其可能是值得进一步关注的候选Th细胞表位。既往关于profilin的研究更多集中于IgE交叉识别现象，缺乏对其交叉表位的深入探究［14］。本研究的结构定位结果可见，候选CD4＋ T细胞表位大多位于相对稳定且具有一定可及性的结构区段，与其他相关研究一致，其原因可能与抗原加工及MHC II呈递通常偏好相对稳定片段的规律相关［15-16］。
从序列比对结果来看，Art v 4与Pru p 4之间呈现出较高的序列保守性，且候选区段存在重叠现象，提示二者可能存在共享的免疫识别基础。这一发现与profilin型过敏原的已有认知相契合。已有研究表明，profilin参与多种花粉-食物交叉反应组合，如Bet v 2（桦树）-Mal d 4（苹果）、Bet v 2（桦树）-Pru p 4（桃）、 Phl p 12（禾本科）-Mal d 4（苹果）等［17］。本研究对Art v 4-Pru p 4的分析，有助于从候选共享表位角度补充艾蒿-桃相关profilin通路的分子层面证据。
血清学实验为上述推测提供了初步支持。桃提取物竞争抑制实验结果表明，桃提取物中存在能够与天然艾蒿提取物竞争患者IgE结合的成分，支持二者在患者血清水平上具有一定程度的交叉反应性。在血清学策略方面，本研究采用混合血清进行初筛，主要目的是提高共有IgE识别位点的检出效率。已有研究在过敏原初筛、IgE immunoblot和dot blot分析中使用混合血清池进行初步血清学评价［18-19］，其优势在于整合多位过敏患者的IgE反应谱，用于比较总体IgE反应性或初筛共有识别趋势，但同时，其难以反映患者之间异质性的不足。这提示后续研究需结合个体血清进行更细致的验证，以全面深入了解表位与个体免疫反应之间的关系。
本研究也存在一些局限性。目前得到的T细胞表位结论仍属于预测性结果，后续若要明确其在实际免疫应答中的作用，还需结合外周血单个核细胞（Peripheral Blood Mononuclear Cells，PBMC）增殖实验、酶联免疫斑点技术（Enzyme Linked Immunospot Assay，ELISPOT）等进行进一步验证。其次，本研究的血清学实验样本量较小且获取范围有限，可能导致研究结果在推广应用时存在一定偏差，未来还需纳入全国不同地区过敏患者的数据。最后，A71区段虽在预测和结构分析中表现出一定候选意义，但由于肽段纯度不足，未能纳入后续免疫学验证，其功能相关性仍需后续研究进一步确认。
综上，本研究初步预测并筛选了Art v 4的候选线性B细胞表位及T细胞表位，并结合血清学实验对部分候选IgE识别区段进行了初步验证。序列比对与结构分析提示，Art v 4与Pru p 4之间具有较高保守性，31~45 aa和101~115 aa区段可能构成二者共享识别的候选基础。结合桃提取物竞争抑制实验，本研究进一步提示艾蒿与桃来源profilin之间可能存在一定交叉反应性。上述结果为进一步解析艾蒿-桃相关profilin通路的分子机制提供了候选区段和实验线索，也可为相关分子诊断及后续干预研究提供参考。
基金
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  • 国家自然科学基金专项项目(32541102)
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2026年第53卷第12期
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doi: 10.20043/j.cnki.MPM.202602163
  • 接收时间：2026-02-14
  • 首发时间：2026-09-28
  • 出版时间：2026-06-25
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  • 收稿日期：2026-02-14
基金
国家自然科学基金专项项目(32541102)
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    中国农业大学食品科学与营养工程学院，北京 100083

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车会莲，E-mail:
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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