Article(id=1311349964631015863, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, articleNumber=null, orderNo=null, doi=10.20043/j.cnki.MPM.202512046, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764691200000, receivedDateStr=2025-12-03, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790579795271, onlineDateStr=2026-09-28, pubDate=1782316800000, pubDateStr=2026-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790579795271, onlineIssueDateStr=2026-09-28, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790579795271, creator=13701087609, updateTime=1790579795271, updator=13701087609, issue=Issue{id=1311349919793905738, tenantId=1146029695717560320, journalId=1227665162245664772, year='2026', volume='53', issue='12', pageStart='2113', pageEnd='2304', issueExtLink='null', onlineDate='null', pubDate='1782316800000', pubDateStr='2026-06-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790579784582, creator='13701087609', updateTime=1790588408144, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1311386089701597565, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1311386089701597566, tenantId=1146029695717560320, journalId=1227665162245664772, issueId=1311349919793905738, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2188, endPage=2194, ext={EN=ArticleExt(id=1311349964840731064, articleId=1311349964631015863, tenantId=1146029695717560320, journalId=1227665162245664772, language=EN, title=Residues of quinolone antibiotics in fresh and frozen aquatic products and exposure assessment among adult residents,Shanghai, 2017-2024, columnId=1228016572783063333, journalTitle=Modern Preventive Medicine, columnName=Nutrition and Food Hygiene, runingTitle=null, highlight=null, articleAbstract=
Objective

To investigate quinolone antibiotic residues in fresh/frozen aquatic animals in Shanghai and assess dietary intake and health risks for local adults.

Methods

A total of 8 857 samples were collected from breeding, circulation and catering links in Shanghai during 2017-2024. Fourteen quinolone antibiotics were detected by high performance liquid chromatography-tandem mass spectrometry. Combined with “2023 Shanghai Residents’Diet and Health Status Monitoring”data, point and probability estimation were used to evaluate health risks.

Results

The total detection rate of quinolone antibiotics was 26.40% with a total over-limit rate of 4.36%. Enrofloxacin (including ciprofloxacin) had the highest detection rate (26.21%) and over-limit rate (3.91%), while other quinolones had detection and over-limit rates <1%. Top 3 for highest average total quinolone antibiotic residues: freshwater fish, freshwater shrimp, marine fish. Point assessment showed food safety indexes <1 for both general and highly exposed populations, indicating low health risks. Probability assessment revealed P50 and P95 exposure levels of enrofloxacin (including ciprofloxacin) were 1.89 ng/(kg·bw·d) and 139.00 ng/(kg·bw·d), respectively.

Conclusion

Enrofloxacin (including ciprofloxacin) was the main detected quinolone in Shanghai’s fresh/frozen aquatic animals, with freshwater fish more contaminated. However, health risks of quinolone intake via these products for Shanghai adults were relatively low.

, authors=Jing-jin YANG, Dan-ping QIU, Hua CAI, Jia-jie ZANG, Wei LU, Hong LIU, Bao-zhang LUO, authorsList=Jing-jin YANG, Dan-ping QIU, Hua CAI, Jia-jie ZANG, Wei LU, Hong LIU, Bao-zhang LUO, authorCompany=null, correspAuthors=Bao-zhang LUO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1311349966245822917, articleId=1311349964631015863, tenantId=1146029695717560320, journalId=1227665162245664772, language=CN, title=2017—2024年上海市鲜冻水产动物中喹诺酮类抗生素残留及成年居民暴露评估, columnId=1228016573034721597, journalTitle=现代预防医学, columnName=营养与食品卫生, runingTitle=null, highlight=null, articleAbstract=
目的

了解上海市鲜冻水产动物中喹诺酮类抗生素残留水平,评估上海市成年居民膳食喹诺酮类抗生素的暴露水平及其健康风险。

方法

2017—2024年,在上海市养殖、流通、餐饮等环节共采集8 857份鲜冻水产动物样品,采用高效液相色谱-质谱/质谱法检测14种喹诺酮类抗生素的含量。结合“2023年上海市成年居民膳食与健康状况监测”消费量数据,采用点评估及概率评估的方法,估算上海市成年居民经鲜冻水产动物摄入喹诺酮类抗生素的健康风险。

结果

鲜冻水产动物中喹诺酮类抗生素总检出率为26.40%,总超标率为4.36%。其中,恩诺沙星(含环丙沙星)检出率和超标率最高,分别为26.21%和3.91%,其余喹诺酮类抗生素检出率和超标率均低于1%。喹诺酮类抗生素总残留量均值最高的前三位为淡水鱼、淡水虾、海水鱼。点评估结果显示,一般暴露人群、高暴露人群喹诺酮类抗生素的食品安全指数均小于1,健康风险较低。概率评估显示,上海市成年居民经鲜冻水产动物摄入恩诺沙星(含环丙沙星)P50暴露量为1.89 ng/(kg·bw·d)、P95暴露量为139.00 ng/(kg·bw·d)。

结论

上海市售鲜冻水产动物中喹诺酮类抗生素检出以恩诺沙星(含环丙沙星)为主,且淡水鱼污染较多,但上海市成年居民经鲜冻水产动物摄入喹诺酮类抗生素的健康风险较低。

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杨京津(1990—),女,硕士,主管医师,研究方向:食品安全风险监测与评估

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(In Chinese), articleTitle=Research progress on quinolone antibiotic pollution in typical freshwater environments in China, refAbstract=null)], funds=[Fund(id=1311349973791375878, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, awardId=202340302, language=CN, fundingSource=上海市卫生健康委卫生行业临床研究专项(202340302), fundOrder=null, country=null), Fund(id=1311349973866873351, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, awardId=25HC2820704, language=CN, fundingSource=2025年度关键技术研发计划“合成生物学”项目(25HC2820704), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1311349966522646982, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, xref=null, ext=[AuthorCompanyExt(id=1311349966531035591, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, companyId=1311349966522646982, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Municipal Center for Disease Control and Prevention (Shanghai Academy of Preventive Medicine), Division of Health Risk Factors Monitoring and Control/NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, Shanghai 201107, China), AuthorCompanyExt(id=1311349966539424200, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, companyId=1311349966522646982, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海市疾病预防控制中心(上海市预防医学科学院)健康危害因素监测与控制所/国家卫生健康委员会食品安全风险评估与标准研制特色实验室,上海 201107)])], figs=[ArticleFig(id=1311349970645647860, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Fig.1, caption=The annual trends of the total detection rate and total over-limit rate of quinolone antibiotics in fresh and frozen aquatic animals from 2017 to 2024, figureFileSmall=9ISd2nVSypFseAVJelB8uQ==, figureFileBig=wB1jF6kzChrN00QnNUVk1g==, tableContent=null), ArticleFig(id=1311349970708562421, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=图1, caption=2017—2024年鲜冻水产动物中喹诺酮类抗生素总检出率和总超标率年度趋势, figureFileSmall=9ISd2nVSypFseAVJelB8uQ==, figureFileBig=wB1jF6kzChrN00QnNUVk1g==, tableContent=null), ArticleFig(id=1311349970893111798, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Fig.2, caption=Probability distribution map of enrofloxacin (including ciprofloxacin) intake via fresh and frozen aquatic animals among adult residents in shanghai, figureFileSmall=vjA/gqDrk9jGKok+OVr/OQ==, figureFileBig=NxdCHHb0nqJg4eifW6XrCw==, tableContent=null), ArticleFig(id=1311349970968609271, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=图2, caption=上海市成年居民经鲜冻水产动物摄入恩诺沙星(含环丙沙星)的概率分布图, figureFileSmall=vjA/gqDrk9jGKok+OVr/OQ==, figureFileBig=NxdCHHb0nqJg4eifW6XrCw==, tableContent=null), ArticleFig(id=1311349971048301048, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Fig.3, caption=Probability distribution map of enrofloxacin (including ciprofloxacin) intake via freshwater fish among adult residents in shanghai, figureFileSmall=U7kX+PA2TzTdI0wRhrOymQ==, figureFileBig=cJcw+zGl561Nvm5iJjogXw==, tableContent=null), ArticleFig(id=1311349971140575737, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=图3, caption=上海市成年居民经淡水鱼摄入恩诺沙星(含环丙沙星)的概率分布图, figureFileSmall=U7kX+PA2TzTdI0wRhrOymQ==, figureFileBig=cJcw+zGl561Nvm5iJjogXw==, tableContent=null), ArticleFig(id=1311349971211878906, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 1, caption=

Distribution and composition ratio of fresh and frozen aquatic products samples

, figureFileSmall=null, figureFileBig=null, tableContent=
分组频数构成比(%)分组频数构成比(%)
年份食品类别
2017年4805.42 淡水鱼3 50939.61
2018年7508.47 淡水虾1 97522.30
2019年8729.85 淡水蟹1 57817.82
2020年1 33615.08 软体动物1 46316.52
2021年1 47716.68 海水鱼2532.86
2022年1 25414.16 海水虾450.51
2023年1 48416.75 海水蟹340.38
2024年1 20413.59 采样环节
包装类型流通环节(农贸市场)4 44150.14
定型包装1071.21流通环节(商店)2 76431.21
散装8 75098.79流通环节(网店)1 50416.98
养殖环节810.91
餐饮环节670.76
), ArticleFig(id=1311349971291570683, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表1, caption=

鲜冻水产动物的样品分布及构成比

, figureFileSmall=null, figureFileBig=null, tableContent=
分组频数构成比(%)分组频数构成比(%)
年份食品类别
2017年4805.42 淡水鱼3 50939.61
2018年7508.47 淡水虾1 97522.30
2019年8729.85 淡水蟹1 57817.82
2020年1 33615.08 软体动物1 46316.52
2021年1 47716.68 海水鱼2532.86
2022年1 25414.16 海水虾450.51
2023年1 48416.75 海水蟹340.38
2024年1 20413.59 采样环节
包装类型流通环节(农贸市场)4 44150.14
定型包装1071.21流通环节(商店)2 76431.21
散装8 75098.79流通环节(网店)1 50416.98
养殖环节810.91
餐饮环节670.76
), ArticleFig(id=1311349971371262460, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 2, caption=

The residue levels, detection and over-limit situations of different quinolone antibiotics in fresh and frozen aquatic animals in Shanghai from 2017 to 2024

, figureFileSmall=null, figureFileBig=null, tableContent=
污染物名称件数 (μg/kg)中位数(μg/kg)P95(μg/kg)最大值(μg/kg)检出率(%)超标率(%)
恩诺沙星(含环丙沙星)8 85728.97±189.390.5076.005 323.0026.213.91
氧氟沙星8 2380.71±7.540.151.50453.000.580.50
诺氟沙星8 2380.78±13.250.151.501 200.000.080.08
培氟沙星8 2380.63±0.720.151.5022.100.060.06
沙拉沙星8 4380.65±1.510.151.50101.000.120.00
达氟沙星7 9980.41±0.480.151.5018.000.010.00
依诺沙星1 0840.19±1.380.150.1545.700.09-
西诺沙星1 0840.16±0.350.150.1511.700.09-
合计8 85733.84±190.565.0082.955 357.1026.404.36
), ArticleFig(id=1311349972918960637, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表2, caption=

2017—2024年上海市鲜冻水产动物中不同喹诺酮类抗生素残留量及检出、超标情况

, figureFileSmall=null, figureFileBig=null, tableContent=
污染物名称件数 (μg/kg)中位数(μg/kg)P95(μg/kg)最大值(μg/kg)检出率(%)超标率(%)
恩诺沙星(含环丙沙星)8 85728.97±189.390.5076.005 323.0026.213.91
氧氟沙星8 2380.71±7.540.151.50453.000.580.50
诺氟沙星8 2380.78±13.250.151.501 200.000.080.08
培氟沙星8 2380.63±0.720.151.5022.100.060.06
沙拉沙星8 4380.65±1.510.151.50101.000.120.00
达氟沙星7 9980.41±0.480.151.5018.000.010.00
依诺沙星1 0840.19±1.380.150.1545.700.09-
西诺沙星1 0840.16±0.350.150.1511.700.09-
合计8 85733.84±190.565.0082.955 357.1026.404.36
), ArticleFig(id=1311349973023818238, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 3, caption=

The total residues, detection and over-limit situations of quinolone antibiotics in different food categories, sampling stages and packaging types of fresh and frozen aquatic animals in Shanghai from 2017 to 2024

, figureFileSmall=null, figureFileBig=null, tableContent=
组别件数χ2(μg/kg)P95(μg/kg)最大值(μg/kg) χ2(P值)
食物类别626.153(P<0.001)
淡水鱼3 50963.69244.355 357.10
淡水虾1 97527.0661.303 598.35
淡水蟹1 5785.2215.0045.20
海水鱼25314.0121.491 940.35
海水虾452.162.6017.25
海水蟹341.771.801.80
软体动物1 4637.4315.00614.35
采样环节137.940(P<0.001)
农贸市场4 44139.5394.304 340.90
商店2 76436.9895.205 357.10
网店1 50414.0732.452 076.85
餐饮环节673.8515.0019.85
养殖环节817.0512.7545.20
包装类型6.643(P=0.010)
定型包装1075.7215.0046.18
散装8 75034.1983.785 357.10
组别检出数检出率(%)χ2(P值)超标数超标率(%)χ2(P值)
食物类别2 216.252(P<0.001)279.097(P<0.001)
淡水鱼1 84052.443018.58
淡水虾28014.18713.59
淡水蟹392.4730.19
海水鱼9838.7431.19
海水虾36.6700.00
海水蟹00.0000.00
软体动物785.3380.55
采样环节89.632(P<0.001)26.221(P<0.001)
农贸市场1 24027.922184.91
商店81129.341334.81
网店26917.89352.33
餐饮环节1014.9300.00
养殖环节89.8800.00
包装类型19.954(P<0.001)3.934(P=0.047)
定型包装87.4800.00
散装2 33026.633864.41
), ArticleFig(id=1311349973116092927, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表3, caption=

2017—2024年上海市鲜冻水产动物不同食物类别、采样环节、包装类型中喹诺酮类抗生素总残留量及检出、超标情况

, figureFileSmall=null, figureFileBig=null, tableContent=
组别件数χ2(μg/kg)P95(μg/kg)最大值(μg/kg) χ2(P值)
食物类别626.153(P<0.001)
淡水鱼3 50963.69244.355 357.10
淡水虾1 97527.0661.303 598.35
淡水蟹1 5785.2215.0045.20
海水鱼25314.0121.491 940.35
海水虾452.162.6017.25
海水蟹341.771.801.80
软体动物1 4637.4315.00614.35
采样环节137.940(P<0.001)
农贸市场4 44139.5394.304 340.90
商店2 76436.9895.205 357.10
网店1 50414.0732.452 076.85
餐饮环节673.8515.0019.85
养殖环节817.0512.7545.20
包装类型6.643(P=0.010)
定型包装1075.7215.0046.18
散装8 75034.1983.785 357.10
组别检出数检出率(%)χ2(P值)超标数超标率(%)χ2(P值)
食物类别2 216.252(P<0.001)279.097(P<0.001)
淡水鱼1 84052.443018.58
淡水虾28014.18713.59
淡水蟹392.4730.19
海水鱼9838.7431.19
海水虾36.6700.00
海水蟹00.0000.00
软体动物785.3380.55
采样环节89.632(P<0.001)26.221(P<0.001)
农贸市场1 24027.922184.91
商店81129.341334.81
网店26917.89352.33
餐饮环节1014.9300.00
养殖环节89.8800.00
包装类型19.954(P<0.001)3.934(P=0.047)
定型包装87.4800.00
散装2 33026.633864.41
), ArticleFig(id=1311349973208367616, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 4, caption=

The consumption of fresh and frozen aquatic animals by different food categories and different groups of residents in Shanghai in 2023

, figureFileSmall=null, figureFileBig=null, tableContent=
组别人数 (g/d)±s(g/d)P95(g/d)F值P值
食物类别444.240<0.001
淡水鱼2 92032.97±51.03a112.86
海水鱼2 92015.84±35.03b57.14
虾2 92019.50±31.62c64.29
蟹2 9206.36±15.79d28.57
软体动物-贝壳类2 9204.94±15.37d24.64
软体动物-其他2 9201.84±7.35e7.14
年龄3.7900.023
18~4473888.87±112.94a275.64
45~5972283.90±98.95a,c223.60
≥601 46076.54±99.34c233.97
性别0.1600.690
男1 45882.24±101.46234.00
女1 46280.71±104.44255.16
地区15.380<0.001
城市中心区1 48091.84±113.73a288.31
城郊结合区72070.25±85.67c190.22
远郊地区72071.39±92.90c215.79
), ArticleFig(id=1311349973313225217, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表4, caption=

上海市不同食物类别和不同人群居民鲜冻水产动物消费量

, figureFileSmall=null, figureFileBig=null, tableContent=
组别人数 (g/d)±s(g/d)P95(g/d)F值P值
食物类别444.240<0.001
淡水鱼2 92032.97±51.03a112.86
海水鱼2 92015.84±35.03b57.14
虾2 92019.50±31.62c64.29
蟹2 9206.36±15.79d28.57
软体动物-贝壳类2 9204.94±15.37d24.64
软体动物-其他2 9201.84±7.35e7.14
年龄3.7900.023
18~4473888.87±112.94a275.64
45~5972283.90±98.95a,c223.60
≥601 46076.54±99.34c233.97
性别0.1600.690
男1 45882.24±101.46234.00
女1 46280.71±104.44255.16
地区15.380<0.001
城市中心区1 48091.84±113.73a288.31
城郊结合区72070.25±85.67c190.22
远郊地区72071.39±92.90c215.79
), ArticleFig(id=1311349973392916994, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 5, caption=

Dietary exposure point assessment of quinolone antibiotics in fresh and frozen aquatic animals

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抗生素ADI
μg/(kg·bw·d)
EDI1
ng/( kg·bw·d)
IFS1EDI2
ng/( kg·bw·d)
IFS2
恩诺沙星(含环丙沙星)6.2108.550.017 5136.490.005 89
沙拉沙星0.32.440.008 130.820.002 73
氧氟沙星5.02.660.000 530.890.000 18
诺氟沙星14.02.920.000 210.980.000 07
达氟沙星20.01.540.000 080.520.000 03
培氟沙星-2.36-0.79-
依诺沙星-0.71-0.24-
西诺沙星-0.60-0.20-
), ArticleFig(id=1311349973485191683, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表5, caption=

鲜冻水产动物中喹诺酮类抗生素的膳食暴露点评估

, figureFileSmall=null, figureFileBig=null, tableContent=
抗生素ADI
μg/(kg·bw·d)
EDI1
ng/( kg·bw·d)
IFS1EDI2
ng/( kg·bw·d)
IFS2
恩诺沙星(含环丙沙星)6.2108.550.017 5136.490.005 89
沙拉沙星0.32.440.008 130.820.002 73
氧氟沙星5.02.660.000 530.890.000 18
诺氟沙星14.02.920.000 210.980.000 07
达氟沙星20.01.540.000 080.520.000 03
培氟沙星-2.36-0.79-
依诺沙星-0.71-0.24-
西诺沙星-0.60-0.20-
), ArticleFig(id=1311349973581660676, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=EN, label=Table 6, caption=

Probability assessment parameters for the consumption of various fresh and frozen aquatic animals

, figureFileSmall=null, figureFileBig=null, tableContent=
水产动物类别分布模型参数
淡水鱼RiskPareto2(1.185,3.316)
海水鱼RiskGamma(0.774,0.391)
虾RiskExpon(0.301)
蟹RiskExpon(0.098)
软体动物RiskExpon(0.102)
), ArticleFig(id=1311349973669741061, tenantId=1146029695717560320, journalId=1227665162245664772, articleId=1311349964631015863, language=CN, label=表6, caption=

各类鲜冻水产动物消费量概率评估参数

, figureFileSmall=null, figureFileBig=null, tableContent=
水产动物类别分布模型参数
淡水鱼RiskPareto2(1.185,3.316)
海水鱼RiskGamma(0.774,0.391)
虾RiskExpon(0.301)
蟹RiskExpon(0.098)
软体动物RiskExpon(0.102)
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2017—2024年上海市鲜冻水产动物中喹诺酮类抗生素残留及成年居民暴露评估
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杨京津 , 邱丹萍 , 蔡华 , 臧嘉捷 , 陆伟 , 刘弘 , 罗宝章
现代预防医学 | 营养与食品卫生 2026,53(12): 2188-2194
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现代预防医学 |营养与食品卫生 2026 , 53 (12) : 2188 -2194
2017—2024年上海市鲜冻水产动物中喹诺酮类抗生素残留及成年居民暴露评估
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杨京津, 邱丹萍, 蔡华, 臧嘉捷, 陆伟, 刘弘, 罗宝章
作者信息
  • 上海市疾病预防控制中心(上海市预防医学科学院)健康危害因素监测与控制所/国家卫生健康委员会食品安全风险评估与标准研制特色实验室,上海 201107
通讯作者:
罗宝章,Email:
作者简介:

杨京津(1990—),女,硕士,主管医师,研究方向:食品安全风险监测与评估

Residues of quinolone antibiotics in fresh and frozen aquatic products and exposure assessment among adult residents,Shanghai, 2017-2024
Jing-jin YANG, Dan-ping QIU, Hua CAI, Jia-jie ZANG, Wei LU, Hong LIU, Bao-zhang LUO
Affiliations
  • Shanghai Municipal Center for Disease Control and Prevention (Shanghai Academy of Preventive Medicine), Division of Health Risk Factors Monitoring and Control/NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, Shanghai 201107, China
出版时间: 2026-06-25 doi: 10.20043/j.cnki.MPM.202512046
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目的

了解上海市鲜冻水产动物中喹诺酮类抗生素残留水平,评估上海市成年居民膳食喹诺酮类抗生素的暴露水平及其健康风险。

方法

2017—2024年,在上海市养殖、流通、餐饮等环节共采集8 857份鲜冻水产动物样品,采用高效液相色谱-质谱/质谱法检测14种喹诺酮类抗生素的含量。结合“2023年上海市成年居民膳食与健康状况监测”消费量数据,采用点评估及概率评估的方法,估算上海市成年居民经鲜冻水产动物摄入喹诺酮类抗生素的健康风险。

结果

鲜冻水产动物中喹诺酮类抗生素总检出率为26.40%,总超标率为4.36%。其中,恩诺沙星(含环丙沙星)检出率和超标率最高,分别为26.21%和3.91%,其余喹诺酮类抗生素检出率和超标率均低于1%。喹诺酮类抗生素总残留量均值最高的前三位为淡水鱼、淡水虾、海水鱼。点评估结果显示,一般暴露人群、高暴露人群喹诺酮类抗生素的食品安全指数均小于1,健康风险较低。概率评估显示,上海市成年居民经鲜冻水产动物摄入恩诺沙星(含环丙沙星)P50暴露量为1.89 ng/(kg·bw·d)、P95暴露量为139.00 ng/(kg·bw·d)。

结论

上海市售鲜冻水产动物中喹诺酮类抗生素检出以恩诺沙星(含环丙沙星)为主,且淡水鱼污染较多,但上海市成年居民经鲜冻水产动物摄入喹诺酮类抗生素的健康风险较低。

水产动物  /  喹诺酮类  /  膳食暴露  /  风险评估
Objective

To investigate quinolone antibiotic residues in fresh/frozen aquatic animals in Shanghai and assess dietary intake and health risks for local adults.

Methods

A total of 8 857 samples were collected from breeding, circulation and catering links in Shanghai during 2017-2024. Fourteen quinolone antibiotics were detected by high performance liquid chromatography-tandem mass spectrometry. Combined with “2023 Shanghai Residents’Diet and Health Status Monitoring”data, point and probability estimation were used to evaluate health risks.

Results

The total detection rate of quinolone antibiotics was 26.40% with a total over-limit rate of 4.36%. Enrofloxacin (including ciprofloxacin) had the highest detection rate (26.21%) and over-limit rate (3.91%), while other quinolones had detection and over-limit rates <1%. Top 3 for highest average total quinolone antibiotic residues: freshwater fish, freshwater shrimp, marine fish. Point assessment showed food safety indexes <1 for both general and highly exposed populations, indicating low health risks. Probability assessment revealed P50 and P95 exposure levels of enrofloxacin (including ciprofloxacin) were 1.89 ng/(kg·bw·d) and 139.00 ng/(kg·bw·d), respectively.

Conclusion

Enrofloxacin (including ciprofloxacin) was the main detected quinolone in Shanghai’s fresh/frozen aquatic animals, with freshwater fish more contaminated. However, health risks of quinolone intake via these products for Shanghai adults were relatively low.

Aquatic animals  /  Quinolone  /  Dietary exposure  /  Risk assessment
杨京津, 邱丹萍, 蔡华, 臧嘉捷, 陆伟, 刘弘, 罗宝章. 2017—2024年上海市鲜冻水产动物中喹诺酮类抗生素残留及成年居民暴露评估. 现代预防医学, 2026 , 53 (12) : 2188 -2194 . DOI: 10.20043/j.cnki.MPM.202512046
Jing-jin YANG, Dan-ping QIU, Hua CAI, Jia-jie ZANG, Wei LU, Hong LIU, Bao-zhang LUO. Residues of quinolone antibiotics in fresh and frozen aquatic products and exposure assessment among adult residents,Shanghai, 2017-2024[J]. Modern Preventive Medicine, 2026 , 53 (12) : 2188 -2194 . DOI: 10.20043/j.cnki.MPM.202512046
过去三十年,中国水产养殖业快速发展,鱼类产量已占全球供应量的三分之一以上[1]。渔业资源有限,集约化、高密度养殖模式虽能提高产量与利润,却也导致水产动物疾病和死亡率上升。因此,养殖户使用抗生素来预防、治疗疾病以及促进生长。而抗生素的长期、广泛、不规范使用易增加水产动物中抗生素的残留风险,最终通过饮食摄入蓄积在人体中;此外,环境中抗生素通过食物链传递、富集作用重新进入动物和人体,形成恶性循环,对人类健康产生不良影响,例如恶心、呕吐等胃肠道刺激、头晕、头痛、过敏反应以及潜在的特殊毒性风险[2-3]。
喹诺酮类抗生素因其广谱、强效、使用方便、不良反应少的特点广泛用于水产养殖,尽管GB 31650-2019《食品安全国家标准食品中兽药最大残留限量》对水产动物中部分喹诺酮类抗生素的残留限量作出规定,但此类抗生素在水产动物中检出率仍处于较高水平[4-5]。
为了解上海市市售鲜冻水产动物中喹诺酮类抗生素的污染状况及膳食暴露风险,本研究对2017—2024年上海市养殖、流通、餐饮等环节共8 857件鲜冻水产动物样品中喹诺酮类抗生素的残留状况进行分析,同时评估上海市成年居民经鲜冻水产动物摄入喹诺酮类抗生素的膳食暴露风险,为抗生素污染控制和合理用药指导提供科学依据和数据支持。
2017—2024年,共采集上海市16个区市售鲜冻水产动物(包括鲜活、冷鲜、冷冻水产动物)样品共8 857份,样品主要来源于流通环节,样品类别包括淡水鱼、虾、蟹、软体动物及少部分海水动物产品(见表1)。样品采集后取可食部分进行匀浆,-20 ℃冷冻保存。
按照《国家食品污染物和有害因素风险监测工作手册》中《动物源性食品中喹诺酮药物残留量测定的标准操作程序》开展样品处理和检测(高效液相色谱-质谱/质谱测定),用阴性样品基质加标外标法定量。检出限为0.30 μg/kg,定量限为1.00 μg/kg。检测指标包括恩诺沙星、环丙沙星、氧氟沙星、诺氟沙星、培氟沙星、沙拉沙星、依诺沙星、西诺沙星、达氟沙星、二氟沙星、噁喹酸、氟甲喹、洛美沙星、萘啶酸共14种喹诺酮类抗生素。其中,恩诺沙星在人体内会转化为环丙沙星,国标以两者之和制定了残留限量和抗生素每日允许摄入量(Acceptable Daily Intake,ADI),在分析时以两者之和计,以下简称恩诺沙星(含环丙沙星)。
根据GB 31650-2019《食品安全国家标准 食品中兽药最大残留限量》、GB 31650.1-2022《食品安全国家标准 食品中41种兽药最大残留限量》中最大残留限量开展超标判定。此外,依诺沙星、西诺沙星、萘啶酸尚无相关残留限量标准,不做超标判定。总检出率=喹诺酮类抗生素检出总件数/水产动物样品总数;按照抗生素种类、水产类别、环节、包装类型分组分析时,检出率=每组检出件数/每组样品件数。
本次评估采用的食物消费量数据来自“2023年上海市成年居民膳食与健康状况监测”的食物频率表,成年居民膳食调查均以街道/乡镇为基本抽样单元,根据非农业人口和外来人口比例按城乡[城市中心区(非农人口>70%且外来人口≤35%)、城郊结合区(非农人口>70%且外来人口>35%)、远郊地区(非农人口≤70%)]、年龄(18~44岁、45~59岁、60岁及以上)、性别分层,采用多阶段分层随机抽样方法进行抽样,采用相同的食物频率问卷开展调查,获得有效样本2 920份。
采用联合国粮农组织/世界卫生组织《食品中化学物风险评估的原则和方法》中的评估模型,根据所检测鲜冻水产动物中抗生素残留量和居民消费量计算得到每日抗生素的膳食暴露量。
膳食暴露量(Estimated Daily Intak,EDI)计算公式如下:
式中,EDI为每日抗生素的膳食暴露量,ng/(kg·bw·d);Ci为不同鲜冻水产动物的消费量,g/d;Ri为鲜冻水产动物中某种抗生素残留量,μg/kg;BW为个体的体重,kg。
高暴露主要用于评估高消费量人群长期摄入污染水平一般的食物带来的风险;一般暴露主要用于评估消费水平一般人群长期摄入污染水平一般的食物带来的风险。EDI1(高暴露人群)=膳食消费量P95*抗生素残留量均值/平均体重;EDI2(一般暴露人群)=膳食消费量均值*抗生素残留量均值/平均体重。
通过Excel软件(V 16.0)建立消费量和抗生素污染含量数据库,采用概率评估软件@Risk 7.5(美国Palisade公司)进行分布拟合,用赤池检验方法进行拟合度检验,并确定最佳拟合分布。应用膳食暴露量公式计算,采用拉丁超立方抽样,以蒙特卡洛原理进行10 000次模拟,最终获得上海市成年居民经鲜冻水产动物(淡水鱼)摄入恩诺沙星(含环丙沙星)的暴露分布。
采用食品安全指数(Index of Food Safety,IFS)法[6-7]来评估鲜冻水产动物中喹诺酮类抗生素的健康风险。IFS1=EDI1/(1 000*ADI)、IFS2=EDI2/(1 000*ADI),EDI单位为ng/(kg·bw·d),ADI单位为μg/(kg·bw·d)。IFS≤1时,表明通过膳食途径摄入抗生素对人体健康影响的风险是可以接受的;当IFS>1时,表明存在发生对健康不利影响的风险,并且这种风险随着数值的增大而增大。
本研究数据采用SAS OA软件进行统计分析,污染物以均数、P95、最大值进行描述,未检出值以1/2 LOD计。不同食品类别、环节、包装类型中抗生素残留量比较采用Kruskal-Wallis非参数检验,检出率和超标率比较采用列联表χ2检验。不同食物类别、年龄、性别、城乡之间的消费量比较采用方差分析,再用SNK-q检验进行两两比较,检验水准为α=0.05。
通过2017—2024年连续监测,鲜冻水产动物中喹诺酮类抗生素总检出率为26.40%(2 338/8 857),总超标率为4.36%(386/8 857),其变化趋势见图1。其中,总检出率总体呈上升趋势,2023年总检出率最高为34.50%,2017年总检出率最低为12.50%。总超标率总体趋势较稳定,2021年总超标率最高为6.30%,2017年总超标率最低为2.90%。
鲜冻水产动物中各种喹诺酮类抗生素残留情况见表2。14种喹诺酮类抗生素中,恩诺沙星(含环丙沙星)检出率最高为26.21%,检出浓度均值为28.97 μg/kg,最大值为5 323.00 μg/kg。其余喹诺酮类抗生素检出率均低于1%。此外,二氟沙星、噁喹酸、氟甲喹、洛美沙星、萘啶酸在鲜冻水产动物中均未检出,后续不做分析与评估。恩诺沙星(含环丙沙星)超标率最高为3.91%,其余喹诺酮类抗生素超标率均低于1%。
不同类别鲜冻水产动物中喹诺酮类抗生素总残留量(χ2=626.153,P<0.001)、检出率(χ2=2 216.252,P<0.001)、超标率均有显著差异(χ2=279.097,P<0.001,见表3)。淡水鱼中总残留量均值最高为63.69 μg/kg。淡水鱼中检出率和超标率最高,分别为52.44%、8.58%。
不同采样环节采集的鲜冻水产动物中喹诺酮类抗生素总残留量(χ2=137.940,P<0.001)、检出率(χ2=89.632,P<0.001)、超标率(χ2=26.221,P<0.001)均有显著差异(见表3)。其中,在农贸市场采集的水产动物样品总残留量均值及超标率最高,分别为39.53 μg/kg、4.91%,在商店采集的水产动物样品检出率最高为29.34%。
散装样品中喹诺酮类抗生素总残留量(χ2=6.643,P=0.010)、检出率(χ2=19.954,P<0.001)、超标率(χ2=3.934,P=0.047)均显著高于定型包装样品(见表3)。散装样品中总残留量均值为34.19 μg/kg、检出率为26.63%、超标率为4.41%。
上海市成年居民对不同类别鲜冻水产动物消费量情况见表4。消费量排序为淡水鱼>虾>海水鱼>蟹≈贝壳类>其他软体动物,成年居民对不同类别鲜冻水产动物消费量差异具有统计学意义(F=444.240,P<0.001)。
上海市不同年龄、性别、地区人群鲜冻水产动物的消费量情况见表4。18~44岁人群鲜冻水产动物消费量显著高于60岁及以上人群(F=3.790,P=0.023);不同性别间鲜冻水产动物的消费量没有显著差异(F=0.160,P=0.690);城市中心区人群鲜冻水产动物消费量显著高于城郊结合区和远郊地区人群(F=15.380,P<0.001)。
本研究分别模拟高暴露人群、一般暴露人群计算各种喹诺酮类抗生素的EDI和IFS,结果见表5。在检出的9种抗生素中,培氟沙星、依诺沙星、西诺沙星因无ADI值,无法计算IFS。不同暴露情况的人群中恩诺沙星(含环丙沙星)的EDI和IFS始终是最高的,恩诺沙星(含环丙沙星)在高暴露人群的IFS为0.017 51,一般人群为0.005 89。表明高暴露人群和一般暴露人群通过鲜冻水产动物摄入喹诺酮类抗生素对人体健康影响的风险是可以接受的。
根据点评估结果,恩诺沙星(含环丙沙星)的膳食暴露量最高,因此选择对恩诺沙星(含环丙沙星)开展概率评估。
对各类鲜冻水产动物中恩诺沙星(含环丙沙星)残留量以及2023年上海市成年居民各类鲜冻水产动物消费量进行分布拟合,恩诺沙星(含环丙沙星)残留量采用离散均匀分布,随机抽取数据。将消费量数据进行拟合度检验,分布模型参数见表6。抽样方法设定为拉丁超立方抽样,以Monte Carlo原理进行10 000次模拟,最终获得上海市成年居民经鲜冻水产动物、淡水鱼摄入恩诺沙星(含环丙沙星)每日暴露量的概率分布。
图2显示,上海市成年居民经鲜冻水产动物摄入恩诺沙星(含环丙沙星)P50暴露量为1.89 ng/(kg·bw·d)、P95暴露量为139.00 ng/(kg·bw·d)。图3显示,上海市成年居民经淡水鱼摄入恩诺沙星(含环丙沙星)P50暴露量为0.41 ng/(kg·bw·d)、P95暴露量为82.40 ng/(kg·bw·d),淡水鱼对经鲜冻水产动物摄入恩诺沙星(含环丙沙星)暴露量的贡献率约为21.69%(根据P50暴露量计算)。
本研究表明,上海市售鲜冻水产动物领域,喹诺酮类抗生素污染问题虽然持续存在,但评估结果表明,一般暴露人群、高暴露人群通过鲜冻水产动物摄入喹诺酮类抗生素对人体的健康风险很低。
本研究中鲜冻水产动物中喹诺酮类抗生素超标率呈现先升后降的变化趋势:2017年起逐步上升,至2021年达到阶段性高点后开始持续回落。这一变化趋势与国家七部委于2021年联合印发的《食用农产品“治违禁控药残 促提升”三年行动方案》(农质发〔2021〕6号)实施时间高度契合,提示该专项整治行动在规范水产养殖用药行为、降低喹诺酮类抗生素残留方面取得了显著成效,为食用农产品质量安全保障提供了有力支撑。
上海市市售鲜冻水产动物中喹诺酮类抗生素检出率处于我国较低水平,低于河南省(30.0%)[8]、山东省(36%)[9]、湖北省(65.0%)[10],稍高于浙江省(20.73%)[5]。我国不同区域水产品抗生素残留水平的差异很大,可能是养殖环境、政策执行力度、养殖模式以及每个城市水产品进货来源不同等多重因素协同作用的结果。此外,不同水产品亚类的抗生素检出率不同,不同研究的水产品种类构成不同可导致抗生素检出率差异。本研究中,喹诺酮类抗生素检出率前三个水产品亚类为淡水鱼(52.44%)>海水鱼(38.74%)>淡水虾(14.18%),与相关研究[5,11]的检出率排序结果一致。
本研究中,喹诺酮类抗生素中恩诺沙星(含环丙沙星)检出率最高,为26.21%,高于江苏省(18.52%)[12]、浙江省(16.64%)[5]、宁波市(13.30%)[13]等地,低于山东省(34.8%)[9]、广州市(45%)[14]、湖北省(62.5%)[10]等地水产品中恩诺沙星的检出率。通过与其他地区的相关研究进行横向对比,能全面了解恩诺沙星在不同区域水产中的残留现状。恩诺沙星作为新一代喹诺酮类抗生素,相比传统抗菌药物,具有抗菌谱广、抑菌能力强、药代动力学特性佳、安全性好、作用靶点独特等显著优势[15]。恩诺沙星在多地水产动物中普遍检出的特征,结合其优良的药剂学特性,凸显了该药物在水产养殖业的应用普遍性及残留管控的必要性。
本研究还存在一定的局限性:喹诺酮类抗生素作为一类常用兽药,广泛存在于水产类、肉类、蛋类、奶类等动物性食品中。本研究在开展暴露评估时,仅将鲜冻水产动物纳入考量范围,导致对喹诺酮类抗生素暴露风险的低估。消费量调查数据显示水产动物消费量在动物性食品(含肉类、蛋类、水产动物类、乳类)消费总量中占比22.10%。经过相应换算后发现,一般暴露和高暴露情况下,经由动物性食品摄入喹诺酮类抗生素的风险仍是可接受的(IFS<1)。此外,软体动物、虾、蟹未按细分类别展开消费量调查,以上不确定性均可能对本次评估结果带来一定影响。
本研究抗生素残留量连续监测时间长、样本量较大,具有较好的代表性;同时采用了具有省级代表性的水产动物消费量,并结合了点评估和概率评估两种方法开展分析。研究结果为食品安全标准优化、养殖环节用药管控及精准化风险干预提供科学依据,进而保障居民饮食安全与健康。
  • 上海市卫生健康委卫生行业临床研究专项(202340302)
  • 2025年度关键技术研发计划“合成生物学”项目(25HC2820704)
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2026年第53卷第12期
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doi: 10.20043/j.cnki.MPM.202512046
  • 接收时间:2025-12-03
  • 首发时间:2026-09-28
  • 出版时间:2026-06-25
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  • 收稿日期:2025-12-03
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上海市卫生健康委卫生行业临床研究专项(202340302)
2025年度关键技术研发计划“合成生物学”项目(25HC2820704)
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    上海市疾病预防控制中心(上海市预防医学科学院)健康危害因素监测与控制所/国家卫生健康委员会食品安全风险评估与标准研制特色实验室,上海 201107

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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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