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[Objective] The conversion of upland to paddy fields and increased fertilizer application have significantly altered soil properties. However, the dynamic evolutionary characteristics and response mechanisms of microbial communities during habitat evolution different years after conversion remain unclear. [Methods] Soil samples were collected from the paddy fields converted from upland fields for different years (0, 3, 8, 15, 20, and 30). Soil physicochemical analysis, real-time quantitative PCR, and high-throughput sequencing were employed to investigate the dynamic changes in soil chemical and biological properties, microbial community composition and asynchrony characteristics, and the interrelationships among these indicators during the habitat evolution following conversion. [Results] As the years after conversion increased, soil organic carbon, total nitrogen, total phosphorus, ammonium nitrogen, and microbial biomass carbon content gradually increased (by 3 to 4 folds), while pH (decreased by up to 0.80) and nitrate content gradually decreased. However, soil potassium content, microbial abundance, and microbial diversity showed no consistent trends. Microbial community analysis revealed that as the years after conversion increased, stress-tolerant genera (Balneola, Flavobacterium, Myxococcus, and Nitrospira) presented enhanced asynchrony and divergence. This optimized interspecies interactions and functional division, thereby improving ecosystem stability. Conversely, increased convergence in genera such as Liberibacter and Variovorax weakened soil functions such as plant growth promotion and pathogen suppression. Correlation analysis indicated that soil pH, organic carbon, and total nitrogen acted as key environmental drivers. Through synergistic and antagonistic interactions, they governed microbial community succession and exerted decisive influences on changes in community asynchrony. [Conclusion] As the years after upland-to-paddy conversion increased, the microbial community asynchrony became enhanced, which improved system stability and reduced carbon losses while compromising soil capacities of plant growth promotion and disease suppression. In the future, strategies such as water management, organic amendment regulation, precision fertilization, and application of synthetic microbial consortia could be employed to directionally enhance microbial divergence and improve ecosystem functional stability.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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