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Deficiency of the ABC transporter permease-like protein DppC2 facilitates the survival of Yersinia pestis in macrophages
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Leiming SHEN1, Yumeng WEI1, 2, Yiming CUI1, 3, Yanting ZHAO1, 4, Wenwu YAO1, Jinping LIU1, 4, Yangyun GUO1, 5, Jiamin LI1, Jiali SHI1, 2, Kai SONG1, Yajun SONG1
Acta Microbiologica Sinica | 2025, 65(9) : 4060 - 4074
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Acta Microbiologica Sinica | 2025, 65(9): 4060-4074
• Research Article •
Deficiency of the ABC transporter permease-like protein DppC2 facilitates the survival of Yersinia pestis in macrophages
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Leiming SHEN1, Yumeng WEI1, 2, Yiming CUI1, 3, Yanting ZHAO1, 4, Wenwu YAO1, Jinping LIU1, 4, Yangyun GUO1, 5, Jiamin LI1, Jiali SHI1, 2, Kai SONG1, Yajun SONG1
Affiliations
  • 1 State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
  • 2 College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China
  • 3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
  • 4 College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
  • 5 School of Basic Medical Sciences, Mudanjiang Medical University, Mudanjiang, Heilongjiang, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250150
Outline
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[Objective] To investigate the role of dppC2 in the survival of Yersinia pestis in macrophages. [Methods] The strain (201-ΔdppC2) with traceless knockout of dppC2 was constructed with a suicide plasmid via homologous recombination based on Y. pestis biovar Microtus strain 201. Phenotypes were compared between 201-ΔdppC2 and the wild type (201-WT) by the acid survival assay, hydrogen peroxide survival assay, macrophage intracellular survival assay, reactive oxygen species (ROS) detection, and cytotoxicity and mouse challenge assays. The gene expression was compared between 201-ΔdppC2 and 201-WT by transcriptomics analysis and RT-qPCR. [Results] Compared with 201-WT, 201-ΔdppC2 exhibited multiple phenotypic alterations, including significantly increases in intracellular survival rates in RAW264.7 and THP-1 cells and under acidic and hydrogen peroxide conditions, upregulation of the acid resistance gene hdeD and the catalase-related genes katA and katG, enhancement of catalase and peroxidase activities, and declines in intracellular ROS levels in 201-ΔdppC2 and RAW264.7 cells infected with the mutant. Furthermore, 201-ΔdppC2 showed reduced cytotoxicity to HeLa cells but no change in the virulence in mice. [Conclusion] The deletion of dppC2 has been demonstrated to enhance the fitness of Y. pestis to acidic and hydrogen peroxide environments, which promote the survival and replication of Y. pestis in macrophages.

Yersinia pestis  /  macrophage  /  dppC2  /  environmental stress tolerance
申雷鸣, 魏雨萌, 崔一鸣, 赵彦婷, 姚文武, 刘瑾萍, 郭阳云, 李嘉敏, 石佳丽, 宋凯, 宋亚军. ABC转运透性酶DppC2的缺失有利于鼠疫耶尔森氏菌在巨噬细胞内的生存. 微生物学报, 2025 , 65 (9) : 4060 -4074 . DOI: 10.13343/j.cnki.wsxb.20250150
Leiming SHEN, Yumeng WEI, Yiming CUI, Yanting ZHAO, Wenwu YAO, Jinping LIU, Yangyun GUO, Jiamin LI, Jiali SHI, Kai SONG, Yajun SONG. Deficiency of the ABC transporter permease-like protein DppC2 facilitates the survival of Yersinia pestis in macrophages[J]. Acta Microbiologica Sinica, 2025 , 65 (9) : 4060 -4074 . DOI: 10.13343/j.cnki.wsxb.20250150
Funding
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  • National Natural Science Foundation of China(U22A20526)
Appendix
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Year 2025 volume 65 Issue 9
617
284
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Article Info
doi: 10.13343/j.cnki.wsxb.20250150
  • Receive Date：2025-02-27
  • Online Date：2026-02-07
  • Published：2025-09-04
Article Data
Affiliations
History
  • Received：2025-02-27
  • Accepted：2025-04-10
Funding
National Natural Science Foundation of China(U22A20526)
Affiliations
    1 State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China
    2 College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China
    3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
    4 College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
    5 School of Basic Medical Sciences, Mudanjiang Medical University, Mudanjiang, Heilongjiang, China

Corresponding:

*E-mail: SONG Kai,
SONG Yajun,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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