Objective To understand the monitoring situation of environmental conditions in inspection and testing institutions, analyze existing problems, and provide corresponding countermeasures. Method A self-designed questionnaire was used to conduct a survey and analysis of laboratory environmental monitoring factors in testing institutions. Result 183 inspection and testing institutions were surveyed, and the environmental conditions involved in the laboratories were mainly concentrated in temperature, humidity, microbial pollution, acidity and alkalinity, toxic corrosion, toxic and harmful gases, etc. 96.17% of the laboratories were involved in temperature and humidity, and 83.06% of the laboratories had already carried out environmental condition monitoring. The main environmental conditions monitored were temperature and humidity, accounting for 86.34%, and less than 25% were monitored for current, voltage, light, pressure, noise, vibration, harmful and toxic gases. Only 18.03% of inspection and testing institutions have carried out information monitoring, while other institutions still carry out monitoring and registration through traditional manual recording. 9.84% of inspection and testing institutions have established data models and trend warnings for inspection and testing environmental conditions. Conclusion Inspection and testing institutions involve a wide variety of environmental conditions during the testing process and have complex monitoring requirements. There are still certain shortcomings in the integration of monitoring technology, dynamic monitoring, and monitoring coverage. It is necessary to establish an environmental condition monitoring information platform, establish a hazard trend warning mechanism through data models, and achieve environmental condition monitoring optimization mechanism and intervention management.

In order to accurately and efficiently determine the purityof ethyl acetate refined products, the separation was carried outaccording to the programmed heating method based on the analysiscondition of gas chromatograph. The initial temperature was set at ${80}^{\circ }\mathrm{C}$ , and after $4\mathrm{\;{min}}$ , the heating ratestarted at ${20}^{\circ }\mathrm{C}/\mathrm{{min}}$ ,and the termination temperature was ${140}^{\circ }\mathrm{C}$ . The analysisshowed that the separation degree of each peak was greater than 2.0, andthe peak shape was symmetrical, sharp and the baseline was stable.Effective separation is possible. After the quantitative analysis ofarea normalization method, correction factor method and internalstandard curve method, the correction factor method has thecharacteristics of high accuracy, strong stability and economy, and itis more suitable for the determination of ethyl acetate purity than thecorrection factor method.

Objective To verify the experimental method of erythromycin ointment. Methods Three batch numbers of erythromycin ointment (S1~S3) were selected and divided into three washing methods: test group and positive and negative control group (test article control group and bacterial solution control group). The microbial limit detection method of erythromycin ointment was analyzed by test. Results The recovery ratio of oxygen bacteria, yeast, mold and added bacteria required for different batches of erythromycin ointment in the test is between 0.5 and 2, and the inhibition of microbial strains of erythromycin ointment test can be adopted by membrane filtration method. Therefore, all the methods can effectively eliminate the antibacterial components of erythromycin ointment, and the experimental results can truly reflect the microbial contamination of erythromycin dye ointment. Conclusion The application of centrifugal method in antibacterial removal will not affect the microbial limit test of erythromycin ointment.

Objective To establish a method for benzo[α]pyrenedetermination in prepared Radix Rehmanniae by molecularly imprintedsolid phase extraction coupled with ratio fluorescence detection. Methods The molecularly imprinted polymers (BaP-MMIPs) were prepared bysol-gel method using dendritic-grafting magnetic nanoparticle as thesupporting material and benzo $\left\lbrack \alpha \right\rbrack$ pyreneas the template. Benzo[ $\alpha$ ]pyrene in the prepared sample wasseparated and enriched by BaP-MMIPs as dispersive SPE materials, and wasdetected by ratio fluorescence determination. The calibration curve wasobtained by measuring the fluorescence intensity ratios $\left({{\mathrm{I}}_{436}/{\mathrm{I}}_{604}}\right)$ of benzo $\left\lbrack \alpha\right\rbrack$ pyrene in different concentrations. ResultsBaP-MMIPs showed remarkable selectivity, high efficiency and stable,which were successful applied as solid phase extraction materials forthe trace benzo[a]pyrene determination in prepared Radix Rehmanniae.Good linearity was achieved in range of 0.14~3.0 $\mu \mathrm{g}/\mathrm{{mL}}$ . The LOD was ${80}\mathrm{{ng}}/\mathrm{{mL}}\left({\mathrm{S}/\mathrm{N}=3}\right)$ and LOQ was ${112.5}\mathrm{{ng}}/\mathrm{{mL}}\left({\mathrm{S}/\mathrm{N}={10}}\right)$ . The average recoveries of benzo $\left\lbrack \alpha \right\rbrack$ pyrenewere 89.5%-106.8% with the RSD≤3.6%. Conclusion The method is accurate,selective, high sensitive, reproducible, economical, simple and fast.The research has profound significance for the benzo[a]pyrene monitoringin Traditional Chinese Medicine.

In response to the issues of inconsistent safety risk management standards, specialized level of safety management, and differentiated control in university laboratories, based on the concept of "full staff education, full process education, and all-round education", this paper proposes the use of information technology to promote laboratory safety classification and grading management measures. Multiple methods such as quantitative, qualitative, or comprehensive evaluation are used to evaluate the degree of risk that may be caused by hazards. Based on the practice of integrating the "Three Comprehensive Education" into the laboratory culture education system of our university, explore the information management mode of safety classification and classification in university laboratories.

Objective To analyze the microbial detection results of drinking water in a certain area. Methods 77 water samples submitted for inspection in 2022 (including 60 for agricultural drinking water and 17 for urban drinking water) and 88 water samples submitted for inspection in 2023 (including 74 for agricultural drinking water and 14 for urban drinking water) were studied. The total number of bacterial colonies, total coliforms and thermostable coliforms were detected according to the Standard Inspection Method for Drinking water. Compare the relevant differences. Results (1) The qualified rate of microbial indexes in 2022 and 2023 was 73.94%; In 2022, 77 water samples were collected, and the total number of bacteria, total coliform, heat-resistant coliform and three comprehensive passrates were 62.34%, 58.44%, 58.44%, 58.44%, respectively. The total number of bacteria, total coliform, thermostable coliform and the comprehensive pass rates of 88 water samples in 2023 were 87.50%, 87.50%, 88.63% and 87.50%, respectively. The total qualified rate of agricultural drinking water in 2022 and 2023 is 56.67% and 60.81%. The total qualified rate of urban drinking water in 2022 and 2023 is 94.12% and 92.86%; In 2022 and 2023, the total number of bacteria, total coliform, heat-resistant coliform and three total pass rates of agricultural drinking water and urban drinking water were compared, and the difference was statistically significant (P＜0.05). Conclusion According to the results of sampling inspection, the qualified rate of urban drinking water is still not 100%, the qualified rateof rural drinking water is lower than that of urban drinking water, there are serious water pollution problems, relevant departments should strengthen the supervision of water quality in the future, and actively improve the disinfection work.

Objective To establish an HPLC method for the simultaneousdetermination of 12 common bacteriostatic agents (benzoic acid, benzylalcohol, sorbic acid, methylparaben, thimerosal, ethylparaben,chlorhexidine acetate, propyl hydroxybenzoate, butyl p-hydroxybenzoate,benzalkonium bromide, domiphen bromide, benzalkonium chloride ) inbrimonidine tartrate eye drops. The 48 batches of samples collected from4 domestic manufacturers were tested and evaluated. Methods ShiseidoCAPCELL PAK C18 MGII column ( ${4.6}\mathrm{\;{mm}}\times {250}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) was used, ${0.02}\mathrm{\;{mol}}/\mathrm{L}$ ammonium acetate(pH adjusted to 4.5 by glacial acetic acid)(mobile phaseA) and methyl alcohol(mobile phase B) were used for gradient elution,and the detection wavelength was ${262}\mathrm{\;{nm}}$ . Results Theseparation degree of 12 bacteriostatic agents reached the requirements,and the blank excipients did not interfere with the determination ofbacteriostatic agents, the linear relationship of bacteriostatic agentsin their linear range was good, the average recoveries were in the rangeof 92.8% to 105.7% and the RSDs were ${0.3}\%\sim {1.9}\%\left({\mathrm{n}=9}\right)$ . The qualitative analysis showed that the types ofbacterial inhibitors in 48 batches were all in agreement with theformulation. The quantitative determination showed that the contents ofbacterial inhibitors were between ${24}\%\sim {102}\%$ of the formulation. Conclusion The method is sensitive, rapid and specific, and can be usedfor the accurate quantitative determination of multiple bacteriostaticagents in brimonidine tartrate eye drops. This study can providetechnical support for the standardized use of bacteriostatic agents inthis variety.

Objective In chemical analysis, the focus used to be on theprecision and accuracy of detection results. However, many testingactivities currently require establishing the credibility of theresults. Especially by measuring the credibility of the results to provetheir suitability, this generally includes the degree to which oneoutcome is expected to match other outcomes, which is also thesignificance of exploring uncertainty. Methods According to theCNAS-GL06 Guidelines for Evaluation of Uncertainty in Chemical Analysis,this article detected the content of phosphate ions in a certainindustrial wastewater and studied and analyzed the components thataffect measurement uncertainty during the experimental process. ResultsThrough calculation, it can be concluded that when the result ofphosphate ion is ${3.00}\mathrm{{mg}}/\mathrm{L}$ , theconfidence interval is ${2.86}-{3.14}\mathrm{{mg}}/\mathrm{L}\left({\mathrm{k}= 2}\right)$ . Conclusion The main factor affecting its measurement uncertainty is theuncertainty introduced by the calibration curve fitting. In order tomake the detection results more scientific and accurate, it is necessaryto pay attention to the maintenance and upkeep of the ion chromatographin daily work, minimize the background noise of the instrument, andimprove the sensitivity and accuracy of the instrument.

In order to further explore the application value of enterprise environmental monitoring laboratories and enhance the feasibility of using laboratory data for self monitoring work, this article analyzes the development status, quality status, and influencing factors of enterprise environmental monitoring laboratories in Dongguan City one by one. At the same time, combined with field research data, real cases are analyzed, and specific reference opinions and suggestions are proposed to promote the continuous improvement of the value of enterprise environmental monitoring laboratories.

In the process of investigating ecological and environmental pollution cases, problems such as difficulty in identifying the attributes of unknown solid waste, complex identification processes, and slow identification time have always affected the efficiency of case investigation. Through personnel interviews, on-site investigations, and initial screening of on-site rapid detection equipment, comprehensive analysis is conducted to determine the detection indicators for the hazardous characteristics of unknown solid volatile organic compounds. The leaching toxicity and toxic substance content of volatile organic compounds are analyzed to identify unknown solid waste as hazardous waste. Accurate identification of hazardous characteristics greatly saves emergency inspection time and provides scientific basis for emergency disposal of unknown solid waste, improves case handling efficiency, and has guiding significance for similar ecological environment damage cases. At the same time, this article also studies the toxicity detection method of volatile organic compounds in solid waste.