Objective To established an analytical method for indirectdetermination of xylo-oligosaccharides in high gelatinization-bakedfoods by high performance liquid chromatography with refractive indexdetector. Methods After degreased with petroleum ether, the samples wereextracted with 20% ethanol solution, and macromolecular sugars such asdextrin and starch were precipitated with 68% ethanol solution. Thesugars in the extraction solution were hydrolyzed into monosaccharidesby diluted sulfuric acid, the solution before and after hydrolysis wasseparated by amide column, detected by refractive index detector, andquantified by external standard method. The product between thedifference of xylose before and after hydrolysis and the averageconversion coefficient of xylo-oligosaccharides and xylose was thexylo-oligosaccharides. Results When the xylose was ${0.25}\sim {5.00}\mathrm{{mg}}/\mathrm{{mL}}$ ,there was a good linear relationship between peak area and concentration $\left({r ={0.9998}}\right)$ . Therecovery rates ranged from 92.0% to 95.8%, the relative standarddeviations (RSDs) were ${1.8}\%\sim {4.0}\%$ in highgelatinization-baked foods. The limit of detection and limit ofquantization of xylo-oligosaccharides were ${0.03}\mathrm{\;g}/{100}\mathrm{\;g},{0.1}\mathrm{\;g}/{100}\mathrm{\;g}$ respectively. Conclusion The extraction method of low concentrationethanol solution is feasible and specific. The method avoided the impactof high gelatinization on the low extraction efficiency and measurementresults. It can determine xylo-oligosaccharides in highgelatinization-baked foods accurately and quickly.

The issue of food quality and safety is a major issue related to the national economy and people's livelihood, and it is also a livelihood issue that the Chinese people are increasingly concerned about. Grain collection and storage enterprises shall establish a quality and safety traceability system to record and save food quality and safety information objectively, effectively and truly. This article aimed to understand the construction and operation status of the traceability system for grain storage enterprises through on-site research, document review, and conference communication. On the basis of drawing on the experience of traceability systems for grain storage enterprises at home and abroad, and in accordance with relevant laws, regulations, and standards such as the Food safety law of the People's Republic of China, the Opinions of the General Office of the State Council on Accelerating the Construction of Important Product Traceability Systems (State Council Office [2015] No.95), and the Opinions of the Food and Drug Administration on Promoting the Improvement of Traceability Systems for Food and Drug Manufacturers and Operators (Food and Drug Administration [2016] No.122), a quality and safety traceability system for grain storage enterprises was formulated, so as torealize the traceability of grain quality and safety in the forward direction, traceability in the reverse direction, and risk control. When quality and safety problems occur, products could be recalled, the causes could be investigated, and responsibilities could be pursued. Intended to provide reference suggestions for improving the quality and safety traceability system construction of grain storage enterprises, to ensure grain quality and safety, and to provide support for supervision.

In view of the current situation of laboratory management in local colleges and universities, in order to realize the reform of informatization of laboratory management system in local colleges and universities, it is proposed to build a lightweight laboratory information sharing platform. This paper analyzes the operation characteristics and existing problems of laboratories in local colleges and universities, and discusses the application value of information technology in laboratory management. On this basis, a low-cost and lightweight information sharing platform is constructed. Combined with the current difficulties of laboratory management in institutions, the platform is divided into four basic modules of instruments and equipment, reagents and consumables, users and information push for construction, in order to enhance the transparency and sharing efficiency of laboratory information and improve the laboratory management mode in local institutions.

Objective Explore the method of using gas-phase molecularabsorption spectroscopy to determine the ammonia content in unorganizedexhaust gas emissions. Methods Explore the detection method from theaspects of detection limit, calibration curve, precision and accuracy,and compare it with the national standard method. Results Theexperimental results showed that there was a good linear relationshipbetween ammonia concentration and absorbance within the range of $0 \sim 2\mathrm{{mg}}/\mathrm{L}$ , with acorrelation coefficient of 0.9999 . The detection limit of the methodwas ${0.1}\mathrm{{\mug}}/{10}\mathrm{\;{mL}}$ . By measuring the standard sample andconducting spiked recovery tests on the actual sample, the relativestandard deviation RSD of the experimental results was ${0.3}\%\sim {1.5}\%$ , the relative errorwas $-{0.1}\%\sim {0.6}\%$ , and thespiked recovery rate was ${97.1}\%\sim {99.5}\%$ . Compared with thenational standard method, there is no significant difference in theresults obtained by this method. Conclusion This method does not requirepre-treatment, does not use highly toxic reagents, has stronganti-interference ability, and can accurately detect the content ofammonia in the unorganized discharge sample. Suitable for determiningthe ammonia content in unorganized emission samples

Objective To investigate the accuracy of chemiluminescenceimmunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) in thedetection of serum markers in patients with HBV infection, and toanalyze the quantitative correlation between serum markers and hepatitisB virus deoxyribonucleic acid (HBV-DNA). Methods A total of 86 patientswith hepatitis B admitted to our hospital from January 2023 to January2024 were selected as subjects. Two immunological detection methods wereused to detect HBV serum markers, and the accuracy of the two detectionmethods was analyzed. Real-time fluorescent polymerase chain reaction(qRT-PCR) was used for quantitative analysis of HBV-DNA load. McNemar’stest was used to analyze the quantitative correlation between serummarkers and HBV-DNA. Results The positive rates of HBsAg, HBsAb andHBeAg were moderate, but the positive rates of HBeAb and HBcAb werestrong. With ELISA as the gold standard, the sensitivity and specificityof CLIA assay were 97.30% and 75.00%, while with CLIA as the goldstandard, the sensitivity and specificity of ELISA assay were 96.00% and81.82%. Among the 6 serum models, the positive rate of HBV-DNA was ${95.65}\%$ , and the mean quantity ofHBV-DNA was ${1.12}\times {10}^{8}$ copies/mL, which was significantly higher than that of the other 5models. The positive rate of HBV-DNA in Xiaosanyang was also relativelyhigh (65.63%). Conclusion The two methods have strong consistency indetecting serum markers of HBV infection, but the

Objective To study the preparation technology anddetermination of compound tetrahydropalmatine pain relief gel. MethodsOrthogonal test was performed to determine the prescription ratio of thegel. RP-HPLC method was adopted. The determination was performed onEclipce XDB-C18 ( ${200}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) column with mobile phase consisted ofmethanol-0.6% glacial acetic acid (pH 6.0) 30 : 70 . The detectionwavelength was ${280}\mathrm{\;{nm}}$ , the flow rate was $1\mathrm{\;{mL}}/\mathrm{{min}}$ .Results With $1\mathrm{\;g}$ carbomer ${940},{20}\mathrm{\;g}$ propyleneglycol, $8\mathrm{\;g}$ glycerol, ${0.5}\mathrm{\;g}$ triethanolamine as thegel matrix, the linear ranges of tetrahydropalmatine and imperatorinwere ${16.1}\sim {96.6}\mathrm{{\mug}}/\mathrm{{mL}}\left({r ={0.9999}}\right)$ and ${10.4}\sim {62.4}\mathrm{{\mug}}/\mathrm{{mL}}$ $\left({r={0.9999}}\right)$ , respectively. The average recoveries were99.13%(RSD was 0.79%) and 99.26%(RSD was 0.82%), respectively.

Objective To improve the precision and accuracy of atomicfluorescence spectrometry for the determination of total seleniumcontent. Methods This article analyzes the factors affecting thedetermination of total selenium in geochemical samples by hydridegeneration atomic fluorescence spectrometry, and explores threeinfluencing factors: sample digestion, instrument cleanliness, andcoexisting ion interference. Optimized the sample digestion process,summarized the issues that should be noted in the instrument during thesample determination process, as well as the common methods foridentifying and eliminating coexisting ion interference. Results Thefeasibility of the method has been verified through 13 national firstlevel standard reference materials, the detection limit of the method is ${0.006}\mathrm{{mg}}/\mathrm{{kg}}$ ,which is lower than the detection limit of DZ/T 0279.14-2016 (0.01mg/kg). The relative standard deviation of the test standard substancewere ${1.9}\%\sim {7.9}\%$ , and $\left|{\Delta\mathrm{{lg}}C}\right|$ were all less than 0.1 . The precisionand accuracy meet the requirements of DZ/T0011-2015 Geochemical CensusSpecification (1:50000). Conclusion Effectively solved the problem ofunstable quality in the determination of total selenium by atomicfluorescence spectroscopy, and improved precision and accuracy.

Objective To evaluate the uncertainty of determination oftotal cadmium (Cd), lead (Pb) and chromium (Cr) in soil by microwavedigestion and inductively coupled plasma mass spectrometry (ICP-MS).Methods To ensure the accuracy and reliability of the detection results,the uncertainty of total cadmium (Cd), lead (Pb) and chromium (Cr) insoil was determined by microwave digestion and inductively coupledplasma mass spectrometry (ICP-MS) and according to JJF1059.1-2012Evalution and Expression of Uncertainty in Measurement the syntheticuncertainty was calculated and the extended uncertainty was obtained.Results The main sources of uncertainty were the standard curvepreparation the repeatability measurement of samples and the standardcurve fitting the contribution rates to the uncertainty of $\mathrm{{Cd}},\mathrm{{Pb}}$ and Cr were50.4%~60.3%, 18.7%~26.1% and 12.1%~20.4%. The influence of sampleweighing and resolution volume for the uncertainty was small and thecontribution rate was between 1.5%~2%, which could be ignored.Conclusion The effective measures to control uncertainty were putforward, which ensured the reliability of the experimental data andprovided the reference for the accurate representation of the heavymetal content in soil

Objective To explore the clinical significance of thecombined detection of fasting blood glucose and glycosylated hemoglobinin the diagnosis and treatment of diabetes. Methods From January 2023 toJanuary 2024,50 healthy people in our hospital were selected as thecontrol group, and 50 diabetes patients in our hospital at the same timewere selected as the observation group. Compare the fasting bloodglucose (FPG), glycated hemoglobin (HbA1c) levels, HbA1c, FPG, and oralglucose tolerance test (OGTT) blood glucose test results, combined andsingle test results after 2 hours of OGTT glucose administration, aswell as the correlation between HbA1c and FPG in the observation group.Results The blood glucose levels, HbA1c, and FPG of the observationgroup after 2 hours of OGTT administration were higher than those of thecontrol group $\left({P<{0.05}}\right)$ . The observation group showed a positivecorrelation between HbA1c and FPG $(r={0.608}$ , $P <{0.05}$ ).The specificity, sensitivity, and accuracy of HbA1c and FPG combineddetection are superior to single detection $\left({P <{0.05}}\right)$ . Conclusion The combined detection of HbA1c and FPG has high diagnostic accuracy fordiabetes, reduces the rate of misdiagnosis and missed diagnosis, canspeed up the diagnosis of diabetes, is conducive to early treatment, andis worth promoting.

Listeria monocytogenes (LM) is a foodborne pathogen that causes diseases with high rates of lethality, and its contamination is particularly problematic in ready-to-eat meat products. With the accelerated pace of modern life and the rapid development of society, the demand for ready-to-eat food has been rising, and the risk of contamination of ready-to-eat food by LM has increased. This not only poses a potential threat to the health and safety of consumers, but also has the potential to trigger significant economic losses. This paper comprehensively analyzed the outbreak cases of LM in ready-to-eat meat products, the relevant laws and regulations of ready-to-eat foods in specific countries, and the current biological control measures. Through the in-depth discussion of relevant elements, the aim was to comprehensively describe the control strategy of LM in ready-to-eat foods, and identified potential risks and challenges, so as to provide scientific guidance for the control of LM in the food industry.