Objective To explore the pathogenic microbial detection results and drug susceptibility results of children with bacterial diarrhea after the collection of stool samples. Methods From April 2020 to June 2023,170 children with bacterial diarrhea participated in the study. The family members were informed about the study, and assisted the children to complete the collection of stool specimens. All the specimens were sent to the laboratory for pathogen culture, counted the distribution of pathogens, and analyzed the drug resistance of pathogens. Results 170 stool samples isolated 122 pathogenic strains, The detection rate of gram-negative bacteria was 76.23%, mainly Salmonella (27.87%), diarrheagenic Escherichia coli (21.31%), Shigella (21.31%); The detection rate of gram-positive bacteria was 23.77%, mainly Staphylococcus aureus (22.95%); Most resistance to tetracycline (41.18%), ciprofloxacin (38.24%), ampicillin (35.29%), The greatest resistance to ciprofloxacin (46.15%), tetracycline (38.46%), of ciprofloxacin (53.85%), tetracycline (46.15%), Staphylococcus aureus had to clindamycin (53.57%) and penicillin (53.57%). Conclusion Salmonella, Escherichia coli, Shigella and Staphylococcus aureus are the main causes of bacterial diarrhea in children. The drug resistance and the choice of sensitive drugs are conducive to the early recovery of children.

Objective To compare the application effects of different influenza virus detection methods. Methods 100 influenza patients admitted to a certain hospital from January 2021 to June 2023 were randomly divided into an observation group (fluorescence RT-PCR test) and a control group (colloidal gold immunochromatographic test), with 50 patients in each group. Results Compared with the control group, the observation group had a higher positive rate of testing (P<0.05). Conclusion Using fluorescence RT-PCR to detect influenza virus can achieve higher detection results and improve the positivity rate of the test.

Objective To determine the inhibitory effect of skin resident bacteria under the action of different preservatives. Methods Combined with the collected real-time data, the minimum inhibitory concentration value (MIC) was calculated first, the initial constraint conditions were set, and then the bacteria were cultured. The change state of the number of colonies was compared in multiple cycles, and the average value of colony change was calculated to determine and analyze the treatment effect of the bacteria at this time. The test solution was diluted batch by batch, and the inhibition and killing pairs of bacteria were calculated according to the comparison of four test targets: aqueous solution, paste cosmetics, powder cosmetics and liquid cosmetics. Results Under different sample concentration, the resident bacteria of skin were killed and inhibited. After multi-level calculation, the final killing pair value was higher, which could reach more than 7.5. Conclusion The inhibitory effect of increasing the concentration of preservatives on resident bacteria in the skin is basically in a positive change relationship. The higher the concentration of preservatives, the better the inhibitory effect will be formed; conversely, the lower the concentration of preservatives, the relatively poor inhibitory effect will be formed.

Objective By analyzing the original detection results of urine organic acids in Changchun, Jilin Province, this study aims to explore the incidence of genetic metabolic diseases related to urine organic acid detection projects in Changchun, Jilin Province, and analyze the detection data. Methods Gas chromatography-mass spectrometry (GC-MS) was used to analyze the results of the urine organic acid detection project submitted by Jilin Jincheng Medical Co., Ltd. in Changchun, Jilin Province from January 2019 to May 2023. Results there were 83 cases of positive diseases in the initial screening, with a positive rate of 3.93%. Among them, there were 43 cases of methylmalonic acidemia with a positive rate of 51.81%, 10 cases of hyperphenylalaninemia with a positive rate of 12.05%, 6 cases of glutaric acidemia type 1 with a positive rate of 7.23%, 5 cases of Citrin protein deficiency with a positive rate of 6.02%, 5 cases of propionaemia with a positive rate of 6.02%, 4 cases of maple diabetes with a positive rate of 4.82%, and 10 cases of other diseases with a positive rate of 12.05%. Conclusion Organic acid metabolism disease, also known as organic aciduria, is the most common genetic metabolic disease. Urine organic acid screening is an effective method for preventing and treating certain genetic metabolic diseases after birth, among which methylmalonic acidemia is the most common genetic metabolic disease with abnormal organic acid metabolism. In order to improve the survival rate and quality of life of children, people must raise awareness, detect, diagnose and treat them early.

Objective To investigate the accuracy of chemiluminescenceimmunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) in thedetection of serum markers in patients with HBV infection, and toanalyze the quantitative correlation between serum markers and hepatitisB virus deoxyribonucleic acid (HBV-DNA). Methods A total of 86 patientswith hepatitis B admitted to our hospital from January 2023 to January2024 were selected as subjects. Two immunological detection methods wereused to detect HBV serum markers, and the accuracy of the two detectionmethods was analyzed. Real-time fluorescent polymerase chain reaction(qRT-PCR) was used for quantitative analysis of HBV-DNA load. McNemar’stest was used to analyze the quantitative correlation between serummarkers and HBV-DNA. Results The positive rates of HBsAg, HBsAb andHBeAg were moderate, but the positive rates of HBeAb and HBcAb werestrong. With ELISA as the gold standard, the sensitivity and specificityof CLIA assay were 97.30% and 75.00%, while with CLIA as the goldstandard, the sensitivity and specificity of ELISA assay were 96.00% and81.82%. Among the 6 serum models, the positive rate of HBV-DNA was ${95.65}\%$ , and the mean quantity ofHBV-DNA was ${1.12}\times {10}^{8}$ copies/mL, which was significantly higher than that of the other 5models. The positive rate of HBV-DNA in Xiaosanyang was also relativelyhigh (65.63%). Conclusion The two methods have strong consistency indetecting serum markers of HBV infection, but the

Lactic acid and its derivatives are important flavor substances in Nongxiang Baijiu which play a vital role in the aroma and taste of the wine. In order to screen lactic acid bacteria, this study tooke a Nongxiang Baijiu fermented grains as the research object. The isolation conditions of lactic acid bacteria were optimized, the effects of different DNA extraction methods were compared, and the species of lactic acid bacteria were identified by enzyme digestion and DNA sequencing. The results showed that 95 strains of lactic acid bacteria were preliminarily screened in MRS broth medium under anaerobic culture at 37°C for 24 h. The optimal method for DNA extraction of lactic acid bacteria was determined to be boiling method, and the strains were identified as Lactobacillus panis, which had the effects of producing lactic acid and n-propanol. This study has important reference value for the production and quality improvement of Nongxiang Baijiu.

Objective To compare the role of enzyme-linkedimmunosorbent assay and toluidine red unheated serum test in thediagnosis of syphilis. Methods 78 suspected syphilis patients in ourhospital were analyzed from January 1st, 2023 to December 31st, 2023,enzyme linked immunosorbent assay and toluidine red unheated serum testwere performed on all patients, and the testing procedures were strictlyfollowed according to the instructions. Compare the diagnosticsensitivity, specificity, and accuracy of enzyme-linked immunosorbentassay (ELISA) and toluidine red unheated serum test based on the resultsof syphilis Treponema pallidum gelatin particle agglutination test.Results The sensitivity, specificity, and accuracy of the enzyme-linkedimmunosorbent assay were 93.24%, 75%, and 92.31%, respectively. Thesensitivity, specificity, and accuracy of the toluene red unheated serumtest were 82.43%, 50%, and 80.77%, respectively. The toluene redunheated serum test was lower than the enzyme-linked immunosorbentassay, with a difference of $P<{0.05}$ . Conclusion Enzyme linked immunosorbent assay(ELISA) has higher sensitivity and accuracy in syphilis testing comparedto the non heated serum test with toluidine red, and is worthy ofattention and promotion.

Objective To study the preparation technology anddetermination of compound tetrahydropalmatine pain relief gel. MethodsOrthogonal test was performed to determine the prescription ratio of thegel. RP-HPLC method was adopted. The determination was performed onEclipce XDB-C18 ( ${200}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) column with mobile phase consisted ofmethanol-0.6% glacial acetic acid (pH 6.0) 30 : 70 . The detectionwavelength was ${280}\mathrm{\;{nm}}$ , the flow rate was $1\mathrm{\;{mL}}/\mathrm{{min}}$ .Results With $1\mathrm{\;g}$ carbomer ${940},{20}\mathrm{\;g}$ propyleneglycol, $8\mathrm{\;g}$ glycerol, ${0.5}\mathrm{\;g}$ triethanolamine as thegel matrix, the linear ranges of tetrahydropalmatine and imperatorinwere ${16.1}\sim {96.6}\mathrm{{\mug}}/\mathrm{{mL}}\left({r ={0.9999}}\right)$ and ${10.4}\sim {62.4}\mathrm{{\mug}}/\mathrm{{mL}}$ $\left({r={0.9999}}\right)$ , respectively. The average recoveries were99.13%(RSD was 0.79%) and 99.26%(RSD was 0.82%), respectively.

Objective To add data output and processing functions to ordinary models of UV visible spectrophotometers. Methods Connect the spectrophotometer to an Android system phone using a serial port to USB cable. The phone uses serial software to receive and process the measurement data sent by the spectrophotometer. Results The connection between the spectrophotometer and the mobile phone was successfully achieved. The mobile phone can smoothly receive and store the output data of the spectrophotometer, and perform graphical processing, synchronously drawing the wavelength absorbance scanning spectrum of the sample. The obtained absorption spectrum is basically consistent with the commercial instrument. Conclusion A simple modification scheme for the output and processing of ordinary spectrophotometer data based on Android system smartphones has been proposed. The modification method is low-cost, easy to operate, and ordinary UV visible spectrophotometers with serial interfaces can be upgraded and modified.

Objective To determine the content of phosphoric ester flame retardants in samples and provide a scientific basis for optimizing the flame retardancy of plastic products. Methods The phosphoric ester flame retardants were extracted from plastic samples with suitable solvent, and the content of phosphoric ester flame retardants was determined by GC-MS. Results The method is fine and can effectively remove impurity interference, can effectively remove the interference of impurities, has a good linearity with a correlation coefficient of more than 0.995; has a good precision with a relative standard deviation of less than 10%; a spiked recovery test was carried out throughout the process, and the recovery rate was 80%~120%. Conclusion This method is accurate and reliable, and can be used for the determination of the content of phosphate ester flame retardants in samples.