Objective This study aims to compare and evaluate theperformance and applicability of two ammonia $\left({\mathrm{{NH}}}_{3}\right)$ detection methods in the field and laboratory. Methods The C16 portable ${\mathrm{{NH}}}_{3}$ gas detectorand GBZ/T 160.29-2004 ammonia Nessler’s reagent spectrophotometricmethod were used to perform 10 repeated measurements of 5 differentconcentrations $\left({{9.3}\sim {148.8}\mathrm{{mg}}/{\mathrm{m}}^{3}}\right)$ of ${\mathrm{{NH}}}_{3}$ standardgases. Results The results showed that the C16 portable detectorexhibited a highly linear relationship $\left({r ={0.9998}}\right)$ in fieldapplications, which was highly correlated with the concentration ofstandard gases; The GBZ/T 160.29-2004 method provides higher precisionmeasurement values in laboratory analysis. The relative standarddeviation and relative error of both methods are less than ±5%,demonstrating good precision and accuracy. The research conclusionindicates that the C16 portable detector is suitable for rapid on-sitedetection and emergency response, while the GBZ/T 160.29-2004 method ismore suitable for laboratory analysis that requires high precision. Conclusion This study provides a scientific basis for selectingappropriate ${\mathrm{{NH}}}_{3}$ detection methods, which is of great significance for improving thequality of environmental monitoring and occupational healthassessment.

Objective A 4-mercaptobenzoic acid/polypeptide-modifiedgold nanoparticle modified surface enhanced Raman spectroscopy (SERS)sensor was developed for rapid detection ofAg ${}^{+ }$ in water. Methods $\;\mathrm{A}{\mathrm{{Ag}}}^{+ }$ surfaceenhanced Raman spectroscopy sensor was constructed by combining aspecificAg ${}^{+ }$ peptiderecognition sequence (RFPRGGDD) with 4-mercaptobenzoic acid, and datawas read using a portable Raman spectrometer. Results In theconcentration range of ${10}\sim {850}\mathrm{{nmol}}/\mathrm{L}$ ,the linear relationship was good, the correlation coefficient $\left({r}^{2}\right)$ was 0.98345, thelimit of detection was ${36}\mathrm{{pmol}}/\mathrm{L}$ , thestandard recoveries were ${95.28}\%\sim {102.9}\%$ , and therelative standard deviations were 0.79%~3.73%. Conclusion The method has high response speed, good selectivity and low detection limit, and is suitable for rapiddetection of Ag+ in water.

Objective This study aims to conduct a compatibility testbetween human fibrinogen and inner packaging materials throughaccelerated testing, explore the stability of drugs, determine whetherinner packaging materials have an impact on product quality, and providenecessary information for formulation design, packaging, transportation,and storage. Methods Conduct accelerated testing using 3 batches of testsamples, and place them upright and inverted at a temperature of $\left({{25}\pm 2}\right){}^{\circ }\mathrm{C}$ for6months. During the experimental period, samples were taken at theend of the third and sixth months respectively, and tested according tothe accelerated stability key inspection items (appearance, vacuumdegree, reconstitution time, visible foreign matter, osmotic pressuremolar concentration, stability test, moisture, $\mathrm{{pH}}$ value, purity, total amountof human fibrinogen, coagulation activity, arginine hydrochloride,citrate ions, sterility test), and compared with the results of Month 0. Results After being stored at ${\left({25}\pm 2\right)}^{\circ }\mathrm{C}$ for6months, the relevant migration products met the requirements, andthere were no significant changes in the activity indicators. Otherbiochemical detection indicators met the requirements of the ChinesePharmacopoeia (2020 edition, Part III), and there were no significantdifferences in the changes of the detection results of each indicator. Conclusion The selected packaging system has no adverse effects on thequality of human fibrinogen, and the drug quality in the selectedpackaging container is stable and controllable, which can maintain itssafety and effectiveness in use. The inner packaging material has nosignificant impact on the quality of the formulation and has goodcompatibility.

Objective To supervise and evaluate the rapid testing laboratory of edible agricultural products, and to check the implementation of local rapid testing. Methods On the basis of the relevant evaluation rules of Guangdong administration for market regulation, combined with the relevant requirements of the government's rapid inspection laboratory operation and management, a comprehensive evaluation of the rapid inspection laboratory was conducted from the aspects of laboratory site, rapid inspection system establishment, completion situation, sampling situation, original records, follow-up treatment, quick inspection products and equipment management. Results The supervision and evaluation of 12 government rapid inspection laboratories for edible agricultural products was carried out, and the comprehensive scores were all above 89 points. Each government rapid inspection laboratory assessed 6 blind samples, only E government rapid inspection laboratory blind sample assessment accuracy rate did not reach 100%. The results of supervision and evaluation were satisfactory. Conclusion The rapid inspection laboratory of local edible agricultural products has achieved certain results in giving play to the 'firewall' of rapid inspection and screening, but there are still some problems and risk points. It is suggested to start with personnel training, original records, rapid inspection products and equipment management, rapid inspection quality control, supervision and assessment, so as to promote the continuous improvement of the detection ability of the rapid inspection laboratory.

Objective To clarify the detection status and antimicrobial sensitivity of clinically isolated Pseudomonas aeruginosa, assist clinical doctors in using antibiotics based on evidence, delay the rate of bacterial resistance, and intervene purposefully in hospital acquired infections. Methods Pseudomonas aeruginosa cultured, isolated, and identified from clinical samples submitted by our company from January 1, 2023 to December 31, 2023 were collected. According to the latest standard CLSI-M100-ED33, the bacteria were identified using a time-of-flight mass spectrometer, and a fully automated identification and drug susceptibility analysis system was used for drug susceptibility testing. Results Pseudomonas aeruginosa was mainly isolated from elderly men over 60 years old. Sputum was the main source of infection specimens of Pseudomonas aeruginosa. The infected patients were mainly distributed in respiratory and internal medicine departments. The isolated Pseudomonas aeruginosa had high resistance to antibiotics such as amtraxam and ciprofloxacin, and high sensitivity to amikacin and tobramycin, and the overall antibiotic resistance showed a downward trend. Conclusion Strict detection of bacterial resistance and drug resistance genes should be carried out in clinical medicine, so that antibiotics can be used more rationally.

University laboratory is an important place for scientific research, and the safety management of laboratory is directly related to the life safety of teachers and students, and will affect the smooth progress of scientific research. However, the traditional management mode has a certain lag, especially in the current situation of more and more types of various tests and more complex, it is very important to actively adopt efficient management to strengthen the management of university laboratory. Capability maturity model is an advanced management concept, which has been gradually introduced into the management of many organizational structures in recent years, and gradually introduced into the management of university laboratories. Therefore, this paper makes an in-depth analysis of university laboratory security management under the guidance of capability maturity model.

Objective To explore the implementation effect of thequality improvement model based on the backtracking system in thehospital inspection department. Methods 102 patients who implemented theconventional quality management model in the inspection department ofthe hospital from September 2021 to September 2022 were selected as thecontrol group, and 102 patients who implemented the quality improvementmodel based on the quality management system in the inspectiondepartment of the hospital from October 2022 to October 2023 wereselected as the observation group. The two groups of inspectiondepartment personnel (24) are all members of the same group.Compare theassessment and scoring of the two groups of inspection departmentpersonnel, the quality of the inspection and the patient satisfaction. Results Compared with the control group, the data obtained showed thatthe theoretical knowledge, operating skills, service quality, equipmentmaintenance and maintenance, equipment use process, and equipmentmanagement ability scores of the inspection department personnel in theobservation group were higher, and the differences were meaningful froma statistical point of view $\left({P<{0.05}}\right)$ ; compared with the control group, thequality inspection effect, safety management, ward management andworking environment scores of the inspection department personnel in theobservation group were higher, and the differences were meaningful froma statistical point of view $\left({P<{0.05}}\right)$ ; Compared with the control group, the dataobtained showed that the patient satisfaction of the observation groupwas higher, and the differences were meaningful from a statistical pointof view $\left({P<{0.05}}\right)$ ; Compared with the control group, thepatient satisfaction of the observation group was higher, and thedifferences were meaningful from a statistical point of view $\left({P <{0.05}}\right)$ ; The dataobtained compared with the control group, the patient satisfaction ofthe observation group was higher, and THE differences were meaningfulfrom a statistical point of view $\left({P<{0.05}}\right)$ . Conclusion The application of the qualityimprovement model based on the backtracking system to the hospitalinspection department can improve the assessment and scoring of itsinspectors and the quality of inspections, and the patient satisfactionis higher and the implementation effect is better.

Spinning disk confocal microscope isa widely used optical instrument. This article take the live cell spinning disk confocal system of the large-scale instrument sharing platform of Wenzhou Medical University as an example, to introduce the basic technical principles and open sharing status of this instrument. Based on actual operating conditions, thisarticle explores the content and methods of training to further improve the effectiveness and feasibility of the training mode, providing a reference for relevant technical personnel.

Objective To investigate the effect of laboratoryinformation management system applied in the quality management oflaboratory department and its impact on the accuracy of specimen testreport. Methods The 1876 specimens that were tested in the laboratorydepartment of our hospital from January 2022 to December 2023 wereincluded in the study, and were divided into two teams according to thedifferent management modes chosen for different time periods; theroutine management was implemented in the laboratory department fromJanuary 2022 to December 2022 ( $n={938}$ , included in the routine team), and the routinemanagement measures were carried out on the basis of the routinemanagement measures in the laboratory department from January 2023 toDecember 2023 ( $n ={938}$ , includedin the surveyteam), to observe the impact of adverse events, test reportsituation and management effect under different management modes. Results Comparing the incidence of adverse events (mislabeling, specimenloss, test failure) between the two teams, the surveyteam (1.81%) waslower $\left({{\chi }^{2}= {7.627}, P<{0.05}}\right)$ ; comparing the specimen test reports betweenthe two teams, the surveyteam had a higher rate of return of criticalvalues, qualified rate of test returns, accurate rate of test reports,and timely rate of critical value reports $\left({P <{0.05}}\right)$ ; Comparisonof the management effect scores of the two teams under differentmanagement modes, the management effect scores of the inspectionpersonnel during the management period were higher in the surveyteam(P<0.05).

Objective To explore and analyze the value of seruminflammatory indexes, D-dimer (D-D) and bone biochemical indexes inwound healing of patients with fractures. Methods The clinical data of92 patients with fracturesin the hospitalwere retrospectively analyzedfrom March 2021 to February 2023. The above patients were divided intodelayed group $(n ={47}$ , delayedfracture healing) and normal group $(n={47}$ , normal fracture healing). The levels of high-sensitivityC-reactive protein (hs-CRP), procalcitonin (PCT), D-D and bonebiochemical indexes (bone gla protein (BGP), type I procollagen aminoterminal peptide (PINP), type I collagen cross-linked carboxy terminalpeptide (CTX), bone alkaline phosphatase (BALP) were compared betweengroups. Pearson analysis was used to analyze the correlation between theabove indexes and delayed fracture healing. Receiver operatingcharacteristic curve (ROC) was adopted to evaluate the predictive valueof the above indexeson predicting delayed fracture healing. Results Thelevels of hs-CRP, PCT, D-D and CTX in delayed group were higher thanthose in normal group $\left({P<{0.05}}\right)$ while the levels of BGP, PINP and BALP werelower than those in normal group $\left({P<{0.05}}\right)$ . Serum hs-CRP, PCT, D-D and CTX in patientswith fractures were positively correlated with delayed fracture healing $\left({P <{0.05}}\right)$ , andBGP, PINP and BALP were negatively correlated with delayed fracturehealing $\left({P<{0.05}}\right)$ . Under the ROC curve, the areas under thecurves (AUCs) of hs-CRP, PCT, D-D, CTX, BGP, PINP, and BALP inpredicting delayed fracture healing were 0.693, 0.810, 0.722, 0.763,0.746, 0.868 and 0.839, the sensitivities were 59.57%, ${59.57}\%,{72.34}\%,{59.57}\%,{85.11}\%,{93.62}\%$ and 76.60%, and the specificities were 78.72%,97.87%,65.96%,89.36%,59.57%, 72.34% and 80.85% respectively. Conclusion hs-CRP, PCT, D-D andbone biochemical indexes are closely related to delayed fracture healingin patients with fractures, which should be paid attention to inclinical practice.