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Objective The aim of this study is to investigate the variation of SERS effect intensity with the distance between probe molecules and the enhanced surface using atomic layer deposition (ALD) technology. Methods The study used ALD technology to deposit two-dimensional materials with different thicknesses on the surface of SERS to block raman probe molecules from the surface, exploring the weakening effect of different interaction distances on the SERS effect of the surface, and studying the changes in raman spectral intensity of probe molecules;the study used a silver-silicon combination as the SERS substrate, and a crystal violet aqueous solution with a concentration of ${10}^{-4}\mathrm{\;{mol}}/\mathrm{L}$ as a raman probe to conduct SERS effect exploration experiments on two-dimensional materials with different thicknesses. Results The final experimental results prove that the liquid SERS spectral intensity at the ${532}\mathrm{\;{nm}}$ laser wavelength generally weakens with the increase in the thickness of the two-dimensional material. Conclusion 5 nm thickness is its limiting distance.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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