Objective The accuracy of the test results was evaluated by the interlaboratory comparison of the blind samples, and the test quality and test ability were improved. Methods According to the “National Standard for Food microbiology inspection of food safety” and other Methods for the 2012-2023 assessment of blind samples for testing. Results During the 2012-2023 Blind examination in Yizheng’s CDC Microbiology Laboratory, 3 strains of Enterocoliform Yersinia, 5 strains of Bacillus cereus, 4 strains of Klebsiella (E. sakazakii) and 2 strains of Enterobacter cloacae were detected, 3 strains of Enterobacter aerogenes, 3 strains of Streptococcus hemolyticus, 1 strain of Streptococcus $A,2$ strains of Streptococcus $B,3$ strains of salmonella,1 strain of salmonella enteritidis, 2 strains of Salmonella enterica, two strains of Liszt. Escherichia coli, Shigella, Citrobacter freundii, Pseudomonas aeruginosa, Vibrio alginolyticus, Vibrio vulnificus, Vibrio parahaemolyticus, Staphylococcus epidermidis. Conclusion From 2012 to 2023, all the results were correct, and the combination of manual and instrument can improve the detection capability and quality of the laboratory.