Objective The color analysis equipment "plasma colorimeter" developed by customization was used to analyze the color of plasma prepared by whole blood composition, summarize the color parameter range of normal, reddish and chyle plasma, and explore the feasibility of formulating quantitative standards for plasma appearance. Methods The collected 2U whole blood was randomly selected, the same amount of normal, reddish and chyle plasma was separated and prepared, the proportion of parameters of red, green and blue in the appearance of plasma was collected by plasma colorimeter, the laws of each parameter were summarized by statistical methods, and the differences between the three plasma parameters were compared. Results Between normal plasma and reddish plasma, the proportion of parameter red was significant different (P>0.05), while those of parameter green and parameter blue were not significant different (P<0.05); Between normal plasma and chyle plasma, the difference of proportion of parameter green was significant (P>0.05), those of parameter red and blue are not significant (P<0.05). Between normal plasma and reddish plasma, the proportions of red, green and blue in reddish plasma and chyle plasma were all significantly different (P<0.05) Conclusions The quantitative values of color in plasma appearance can be obtained by using plasma colorimeter, and these values can provide a basis for digital and accurate judgment of plasma appearance, further to effectively improving the efficiency of blood station work.