Objective To establish a high-performance liquidchromatography method for the detection of dibenzothiazole disulfide(MBTS) in soil. Methods Extract using acetonitrile ultrasound for 30minutes, filter through an organic membrane filter, and purify through aC18 column. Use an HPLC analyzer with set flow rate of ${0.7}\mathrm{\;{mL}}/\mathrm{{min}}$ andcolumn temperature of ${40}^{\circ }\mathrm{C}$ , and the soilsample is separated for qualitative analysis and quantitative analysisusing external standard method at a detection wavelength of ${275}\mathrm{\;{nm}}$ . Results Byoptimizing the pre-treatment and chromatographic conditions for MBTSdetection, acetonitrile was used as the extraction solvent, C18 columnserves as the purification column. An appropriate gradient elutionprogram has a good effect on reducing matrix effects in soil andanalyzing target compounds. Through methodological validation, it wasfound that, the relative standard deviation of MBTS spiked precisionranges from 0.070% to 0.201%. The relative standard deviation of MBTSspiking accuracy ranges from 0.071% to 0.646%, and the spiking recoveryrate ranges from 91.0% to 97.1%, the detection limit of MBTS method is0.003 mg/kg, and the lower limit of determination is ${0.013}\mathrm{{mg}}/\mathrm{{kg}}$ . Conclusion This method has the advantages of simple operation, lowdetection limit, short testing time, low interference, high precisionand accuracy, and can provide technical support for the MBTS insoil.

high performance liquid chromatography  /  MBTS  /  soil