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Objective To establish a method for benzo[α]pyrenedetermination in prepared Radix Rehmanniae by molecularly imprintedsolid phase extraction coupled with ratio fluorescence detection. Methods The molecularly imprinted polymers (BaP-MMIPs) were prepared bysol-gel method using dendritic-grafting magnetic nanoparticle as thesupporting material and benzo $\left\lbrack \alpha \right\rbrack$ pyreneas the template. Benzo[ $\alpha$ ]pyrene in the prepared sample wasseparated and enriched by BaP-MMIPs as dispersive SPE materials, and wasdetected by ratio fluorescence determination. The calibration curve wasobtained by measuring the fluorescence intensity ratios $\left({{\mathrm{I}}_{436}/{\mathrm{I}}_{604}}\right)$ of benzo $\left\lbrack \alpha\right\rbrack$ pyrene in different concentrations. ResultsBaP-MMIPs showed remarkable selectivity, high efficiency and stable,which were successful applied as solid phase extraction materials forthe trace benzo[a]pyrene determination in prepared Radix Rehmanniae.Good linearity was achieved in range of 0.14~3.0 $\mu \mathrm{g}/\mathrm{{mL}}$ . The LOD was ${80}\mathrm{{ng}}/\mathrm{{mL}}\left({\mathrm{S}/\mathrm{N}=3}\right)$ and LOQ was ${112.5}\mathrm{{ng}}/\mathrm{{mL}}\left({\mathrm{S}/\mathrm{N}={10}}\right)$ . The average recoveries of benzo $\left\lbrack \alpha \right\rbrack$ pyrenewere 89.5%-106.8% with the RSD≤3.6%. Conclusion The method is accurate,selective, high sensitive, reproducible, economical, simple and fast.The research has profound significance for the benzo[a]pyrene monitoringin Traditional Chinese Medicine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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