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Objective To optimize the detection method and explore thedetection method with stable strength data and small relative standarddeviation of Curdlan. Method Using Curdlan as the material, compare theglue making methods, optimize the sampling amount, heating temperature,ice water bath cooling time, and parallel measurement times based onsingle factor experiments, and analyze the optimal combination of eachfactor condition through orthogonal experiments. Result The determineddetection method is to take a sample of ${0.30}- {0.31}\mathrm{\;g}$ , add ${15}\mathrm{\;{mL}}$ of freshly prepareddegassed water, stir with a trace sampling spoon to evenly disperse thesuspension, heat in a boiling water bath and maintain its uniformity,heat in a water bath for 10 minutes, then remove and cool in an icewater bath for 50 minutes, and conduct three parallel experiments. Conclusion This method has a small relative standard deviation andstable data. The column heads with diameters of ${0.4}\mathrm{\;{cm}},{0.5}\mathrm{\;{cm}}$ and ${0.6}\mathrm{\;{cm}}$ forproducts with gel strength greater than ${600}\mathrm{\;g}\cdot {\mathrm{{cm}}}^{-2}$ can be used for determination.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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