Objective To establish a highly specific method for thedetermination of related substances in salbutamol sulfate injection. Methods HPLC was performed on a column packed with spherical end-cappedoctylsilyl silica gel (Hypersil BDS C8 ${4.6}\times {150}\mathrm{\;{mm}},{3\mu}\mathrm{m}$ ) at the flow rate of ${0.6}\mathrm{\;{mL}}/\mathrm{{min}}$ , thedetection wavelength of ${220}\mathrm{\;{nm}}$ and columntemperature of ${25}^{\circ }\mathrm{C}$ by gradientelution using ${3.45}\mathrm{\;g}/\mathrm{L}$ sodiumdihydrogen phosphate dihydrate solution (adjust to $\mathrm{{pH}}$ 3.0 with phosphoric acid)as mobile phase A, 10 volumes of methanol and 90 volumes of acetonitrileas mobile phase B. Results The chromatograph showed good specificity ofthe peaks of main components and 15 impurities, and the methodverification of 6 of the impurities showed good linear, repeatabilityand recovery. Conclusion The chromatographic peaks of the maincomponents in salbutamol can be completely separated from the 15impurities. The established method is highly specific, easy to operateand can be used for the inspection of related substances in salbutamolsulfate injection.

salbutamol sulfate  /  injection  /  methods for the detection of substances  /  HPLC