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Objective To establish a new liquid chromatographic methodfor caffeine content in milk tea, to detect the caffeine content ofinstant milk tea in the region, and to make recommendations on healtheffects. Methods The determination was carried out on a Waters highperformance liquid chromatography Symmetry C18 column $\left({{5\mu}\mathrm{m}\times {150}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}}}\right)$ ,the mobile phase was methanol-water $\left({{30}+ {70}}\right)$ at a flow rateof $1\mathrm{\;{mL}}/\mathrm{{min}}$ ,the detection wavelength of ${272}\mathrm{\;{nm}}$ , and the columntemperature was ${35}^{\circ }\mathrm{C}$ . Results Thecorrelation coefficient was $\mathrm{r}={0.9999}$ over the linear range of 1-30 μg/mL, the recoverieswere 97.2%-99.5%, and the method precision was ${0.54}\%$ . Conclusion The method adoptspotassiun ferrocyanide and zinc acetate precipitation centrifugation,which can rapidly extract the effective components of caffeine in milktea and remove the interference of proteins, shorten the pretreatmenttime, optimize the chromatographic conditions, and accelerate thedetection of caffeine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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