Objective Gray mold is an important disease in thestrawberry production process, which seriously affects its yield andquality. This article aims to study the resistance of gray moldpathogens in different strawberry production areas in Zhejiang Provinceto fludioxonil, in order to clarify the current use status and futuremedication strategies of this fungicide. Methods This article uses themycelial growth rate method to conduct indoor toxicity testing on 184strains isolated from strawberry gray mold disease samples in 7 regionsof Zhejiang Province, in order to determine their sensitivity tofludioxonitril and count the frequency of occurrence of resistant andsensitive strains. Results The results showed that the strawberry graymold strain in Quzhou area had the highest sensitivity to fludioxonil,with an average ${\mathrm{{EC}}}_{50}$ value of ${0.014\mu }\mathrm{g}/\mathrm{{mL}}$ ,while the strawberry gray mold strain in Huzhou area had the lowestsensitivity to fludioxonil, with an average ${\mathrm{{EC}}}_{50}$ value of ${0.032\mu }\mathrm{g}/\mathrm{{mL}}$ and asensitivity baseline of ${0.018\mu}\mathrm{g}/\mathrm{{mL}}$ and the sensitive baseline value forthe whole province is ${0.018\mu}\mathrm{g}/\mathrm{{mL}}$ . Conclusion At present, a few lowresistant strains have appeared in the Zhejiang region, indicating thatthe strawberry gray mold fungus has developed certain resistance tofludioxonitril. The sensitivity baseline value has increased compared toprevious research results, but the overall level of drug resistance isstill controllable.

gray mold  /  Botrytis cinerea  /  fludioxonil  /  indoor toxicity