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Objective To systematically control the quality of nucleic acid testing laboratories through the HACCP system, in order to improve the quality of nucleic acid testing laboratories and provide reference for effectively formulating quality control strategies for nucleic acid testing. Methods Preliminary exploration of quality control in nucleic acid testing laboratories based on the HACCP system, with a focus on analyzing the monitoring procedures, validation measures, and corrective measures developed by the HACCP system in the stages of nucleic acid sample reception, preparation of reagents, sample nucleic acid extraction, nucleic acid amplification, result interpretation, and nucleic acid testing result reporting. Results By analyzing the testing data December 2021 to December 2022, the results showed that all batches of nucleic acid positive quality control products met the judgment rules, and each batch of experiments was in a controlled state. Statistical analysis was conducted on laboratory data under the HACCP system, calculating their standard deviation, mean, and coefficient of variation. After plotting the box plot, outlier tests were conducted, and it was found that the mean did not produce outliers. Conclusion The application of HACCP system in nucleic acid testing laboratories can effectively control the quality of nucleic acid testing laboratories, comprehensively control important control points of nucleic acid testing laboratories, improve the level and quality of laboratory biosafety,
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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