Full
Outline
This study aimed to construct a highly sensitive, reliable, and low-cost electrochemical sensor based on graphitic carbon nitride (g-C3N4) modified materials for efficient and convenient detection of purine content in edible mushrooms. The g-C3N4 material was fabricated through a high-temperature calcination method, and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). The results indicated that the g-C3N4 material exhibited a two-dimensional sheet-like nanostructure, characterized by a high degree of graphitization, abundant N-functional group content, and a specific surface area of 61.35 m2/g. An electrochemical sensor (g-C3N4/GCE) was fabricated by modifying a glassy carbon electrode (GCE) with the composite material. Under optimal conditions (pH5.5, enrichment potential of 0.3 V, and enrichment time of 5 min), the peak current of the sensor demonstrated a favorable linear relationship with the concentration of xanthine. Within the low concentration range of 0.5~5 μmol/L, a linear relationship was established as Ipa=0.310CXA+2.859 (where Ipa represented the peak current and CXA denoted the xanthine concentration), with a coefficient of determination R2=0.990. In the high concentration range of 5~200 μmol/L, the linear relationship was determined to be Ipa=0.051CXA+4.034, with R2=0.997. The minimum detection limit (LOD) was calculated to be 0.28 μmol/L. According to this sensor, the xanthine content in shiitake mushrooms, tea tree mushrooms, and wood ears were 307.50, 229.22, and 47.27 mg/100 g, respectively. Compared with the commonly used high-performance liquid chromatography method, the g-C3N4 electrochemical sensor exhibited good stability, with a relative standard deviation (RSD) of 1.45%~3.69%, and the operation was simple, which could be used for the detection of xanthine in edible mushrooms.
82
40
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
