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Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus bulgaricus and Streptococcus thermophilus
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Qingchao JIA1, 2, Huanhuan GU1, Huiyan ZHANG2, *, Yingying BAN2
Science and Technology of Food Industry | 2026, 47(3) : 232 - 243
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Science and Technology of Food Industry | 2026, 47(3): 232-243
• Processing Technology •
Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus bulgaricus and Streptococcus thermophilus
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Qingchao JIA1, 2, Huanhuan GU1, Huiyan ZHANG2, *, Yingying BAN2
Affiliations
  • 1School of Food Science and Engineering, Zhengzhou University of Science and Technology, Zhengzhou 450000, China
  • 2Zhengzhou Ruipu Biological Engineering Co., Ltd., Zhengzhou 450001, China
Published: 2026-02-01 doi: 10.13386/j.issn1002-0306.2025010286
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Objective: The preparation of liquid black garlic by lactic acid bacteria cooperative fermentation and the analysis of aroma components were studied. Methods: Fresh garlic was subjected to high-temperature enzyme inactivation at 180 ℃, then crushed and mixed with water. Different proportions of composite lactic acid bacteria were added for fermentation for a certain period of time. Liquid black garlic was prepared by high-temperature reaction at 80 ℃. The degree of browning, reducing sugar, total phenols, total acids, amino acid nitrogen content and DPPH·, ·OH scavenging rate were measured every two days using liquid black garlic (KB1、KB2) without bacterial fermentation as a reference, and the optimum conditions for preparing liquid black garlic were studied under the fermentation of complex lactic acid bacteria in different proportions, and the aroma components of liquid black garlic under these conditions were analyzed. Results: The best preparation conditions for liquid black garlic were determined as follows: The fermentation time was 9 min, the mass ratio of lactic acid bacteria S (Streptococcus thermophilus):B (Lactobacillus bulgaricus) was 3:1, and the maturation standard of black garlic was reached at 13 d. Under the same conditions, KB1 was used as the standard. At this time, the reducing sugar content was 1.20 times, the total phenol content was 1.82 times, the total acid content was 1.36 times, and the amino acid nitrogen was 1.21 times. DPPH· clearance and ·OH clearance were 1.37 times and 1.34 times, respectively. Compared with single lactic acid bacteria S and B, the thermal reaction was shortened by 2 d and 5 d, respectively. The analysis of aroma components showed that, compared with KB1, the content of thioether in liquid black garlic co-fermented by strains decreased by 54.17%, while the volatile aroma components of aldehydes and furan compounds increased by 47.98% and 6.18% respectively. The liquid black garlic basically had no garlic odor, and the aroma acceptability was better, with a sensory score of 91.38±0.98 points. Conclusion: The fermentation of liquid black garlic by complex lactic acid bacteria has a synergistic effect and can shorten the thermal reaction of black garlic, which has a good application prospect in the development and research of black garlic.

liquid black garlic  /  Lactobacillus bulgaricus  /  Streptococcus thermophilus  /  synergistic fermentation  /  quality indicators
贾庆超, 谷焕焕, 张辉艳, 班莹莹. 保加利亚乳杆菌和嗜热链球菌协同发酵制备液态黑蒜技术研究. 食品工业科技, 2026 , 47 (3) : 232 -243 . DOI: 10.13386/j.issn1002-0306.2025010286
Qingchao JIA, Huanhuan GU, Huiyan ZHANG, Yingying BAN. Preparation Technology of Liquid Black Garlic by Synergistic Fermentation of Lactobacillus bulgaricus and Streptococcus thermophilus[J]. Science and Technology of Food Industry, 2026 , 47 (3) : 232 -243 . DOI: 10.13386/j.issn1002-0306.2025010286
Year 2026 volume 47 Issue 3
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37
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doi: 10.13386/j.issn1002-0306.2025010286
  • Receive Date:2025-02-07
  • Online Date:2026-10-25
  • Published:2026-02-01
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  • Received:2025-02-07
Affiliations
    1School of Food Science and Engineering, Zhengzhou University of Science and Technology, Zhengzhou 450000, China
    2Zhengzhou Ruipu Biological Engineering Co., Ltd., Zhengzhou 450001, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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