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This study investigated the impact of a combination of dynamic high pressure microfluidization (DHPM) and cellulase treatment on the structural and physicochemical properties of pea dietary fiber-caffeic acid (PDF-CA) complex. The experimental model involved subjecting PDF-CA to DHPM, followed by the addition of cellulase, with the objective of examining how the combination of DHPM and cellulase influenced the structure and physicochemical properties of PDF-CA. Scanning electron microscopy (SEM) and particle size analysis revealed that the application of DHPM in conjunction with enzyme treatment led to a reduction in the size of the particles in the samples. The surface of the samples exhibited a rough and loose texture, and agglomeration occurred at an enzyme concentration of 3%. Fourier infrared (FT-IR) and X-ray diffraction (XRD) spectroscopy indicated that the modification did not induce any alterations in the crystal structure of PDF-CA. Additionally, no novel functional groups were detected, although the degree of crystalline order diminished. Compared to untreated samples, modified PDF-CA exhibited improved water- and oil-holding capacities, as well as improved glucose and cholesterol adsorption capacities. Water and oil retention significantly increased at a treatment pressure of 60 MPa with two cycles and an enzyme concentration of 1%, rising from 6.4 g/g and 7.9 g/g to 8.9 g/g and 11.1 g/g respectively, representing increases of 39.1% and 40.5%. The glucose adsorption capacity showed the most pronounced increase after a single treatment at 60 MPa with 3% enzyme concentration, rising from 3.9 mg/g to 13.6 mg/g with an increase of 248.7%. The most significant improvement in cholesterol adsorption capacity was observed after three 60 MPa treatments at a 1% enzyme concentration, with increases from 6.6 g/g (pH2) and 6.7 g/g (pH7) to 14.5 g/g and 13.2 g/g representing respective increases of 119.7% and 97.0%. In summary, the combined use of DHPM and enzymatic modification effectively improved the physicochemical properties of PDF-CA, providing theoretical support for its application in modification and processing of dietary fibre-polyphenol complexes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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