This study aimed to investigate the enrichment effect of cold atmospheric pressure plasma (CAPP) combined with VB6 and VC on gamma-aminobutyric acid (GABA) content enrichment of purple kidney bean germination. The purple kidney beans were used as raw materials. The effects of different concentrations of VB6 and VC on the GABA enrichment of germinated beans treated with cold atmospheric pressure plasma as well as the activities of related metabolic enzymes were investigated. The results demonstrated that the method of cold atmospheric pressure plasma treatment with VB6 and VC promoted the enrichment of GABA in sprouted purple kidney beans. The GABA enrichment content reached 10.05±0.93 mg/g at 72 h of germination when treated with the CAPP combined with 0.25 mg/mL VC. The GABA enrichment content reached 10.09±0.06 mg/g at 72 h germination when treated with CAPP combined with 0.5 mg/mL VB6. Analysis of enzyme activities of germinated purple kidney beans at 72 h revealed that CAPP, VB6 and VC treatments enhanced glutamate decarboxylase (GAD) activity while partially suppressing polyamine oxidase (PAO) activity. The combination of CAPP with VB6 and CAPP with VC for the germination of purple kidney beans both enriched GABA by enhancing the activity of GAD and suppressing the activity of GABA transaminase (GABA-T). The result showed that the treatment condition had a promotion effect on the enrichment of GABA in the germination of kidney beans, and established a theoretical foundation for the production of high GABA rich food.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |