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This study investigated the effects of dynamic high-pressure microfluidization (DHPM) on the structural characteristics and bioactivity of polysaccharides extracted from Dictyophora indusiata (DIP). The physicochemical properties, molecular weight, monosaccharide composition, and Fourier transform infrared (FTIR) spectral features of DIP were systematically analyzed following DHPM treatment. Antioxidant and anti-inflammatory activities were evaluated using DPPH·, ABTS+·, and ·OH scavenging assays, ferric ion reducing antioxidant power (FRAP), and an lipopolysaccharide-induced RAW264.7 macrophage inflammation model. Compared with the group that did not undergo DHPM treatment, DHPM treatment significantly increased the polysaccharide yield and total polysaccharide content, by 4.87-fold and 1.23-fold, respectively (P<0.01). It also markedly reduced the weight-average molecular weight and extremely significantly increased the glucose content in the monosaccharide composition (P<0.001), without altering the primary structure, which remained pyranose ring-based. Additionally, DHPM-treated DIP demonstrated enhanced antioxidant activity, including improved scavenging ability of DPPH·, ABTS+·, and ·OH and greater ferric ion reducing power. The DHPM treatment also significantly downregulated IL-6 and TNF-α expression in LPS-stimulated RAW264.7 cells (P<0.05), indicating stronger anti-inflammatory activity. In conclusion, DHPM-assisted extraction effectively improves the yield and functional properties of DIP by reducing molecular weight and modifying monosaccharide composition. These findings provide valuable insights for optimizing the extraction and functional utilization of DIP.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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