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In order to explore the structural and functional properties of the extracellular polysaccharide flippase (Wzx) from Lactobacillus fermentum A51, this paper systematically investigated the Wzx protein using bioinformatics technology. Firstly, the evolutionary characteristics of the Wzx protein were analyzed through multiple sequence alignment and the construction of a phylogenetic tree. Subsequently, the physicochemical properties, subcellular localization, functional domains, hydrophilicity/hydrophobicity, signal peptides, transmembrane regions, phosphorylation modification sites, glycosylation modification sites, advanced structure, and mode of action were predicted and analyzed. The results indicated that Wzx was composed of 472 amino acids, with a relative molecular weight of 53.31 kDa and a total atom count of 7651. The theoretical isoelectric point was 9.50, and the instability coefficient was 33.79, and it was an alkaline stable hydrophobic protein. Wzx lacked a signal peptide and functioned as a flipping enzyme within the cell membrane. Following transmembrane domain prediction, it was determined that Wzx contained 14 transmembrane helix structures. A total of 33 significant phosphorylation sites were identified, comprising 14 serine (Ser) sites, 12 threonine (Thr) sites, and 7 tyrosine (Tyr) sites. Regarding subcellular localization and functional domain prediction, Wzx was predominantly localized in the endoplasmic reticulum (55.6%), indicating its membership in the Multidrug and Toxic Compound Extrusion family. In terms of the secondary structure of Wzx, α-helix constituted 61.44%, random coil accounted for 22.25%, and extended chain represented 16.31%, Based on the “V” shape of the three-dimensional structure of Wzx, it was speculated that it realized the transport of extracellular polysaccharides through its own conformational reversal or its own conformational change. This study offers a theoretical foundation for a deeper understanding of the functional characteristics and mechanisms of action of Wzx, which is crucial for comprehending the transport processes of extracellular polysaccharides.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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