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To develop a method for the preparation of selenium exopolysaccharide (Se-EPS) with high yield, the heat-resistant Weizmannia coagulans P-L1 was selected to produce Se-EPS through fermentation, with the addition of sodium selenite at a concentration of 20 mg/L, using anion exchange resins. In addition, Se-EPS was characterized using infrared spectroscopy, and in vitro hypoglycemic activity of Se-EPS was investigated. The results showed that the initial bacterial concentration, the employment of anion exchange resins and the fermentation time all had a significant effect on the yield of Se-EPS. With an initial bacterial concentration of 1×106 CFU/mL, the yield of Se-EPS achieved up to 19.1 g/L after 8 days of fermentation, which was increased by 5.4-fold as compared to the yield of Se-EPS obtained with an initial bacterial concentration of 1×105 CFU/mL, and was increased by 19.5-fold as compared to the yield of exopolysaccharide (EPS) obtained by conventional fermentation (without using anion-exchange resin). The selenium content of the Se-EPS reached up to 213 μg/g. Infrared spectroscopy analysis revealed that selenium was probably incorporated into the EPS via Se-O and Se=O bonds. Both EPS and Se-EPS derived from Weizmannia coagulans P-L1 exhibited good in vitro hypoglycemic activity, with Se-EPS showing significantly higher activity than EPS. At a concentration of 1 mg/mL, the inhibition of Se-EPS against α-glucosidase and α-amylase were 28% and 16% higher than those of EPS, respectively. The study provides theoretical support and technical references for the development and application of Se-EPS products derived from Weizmannia coagulans.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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