Full
Outline
To investigate the changes in bioactive compounds and the hypoglycemic action mechanisms of chickpea milk fermented by probiotics, Lacticaseibacillus rhamnosus FMBL L23004 CNN that capable of enhancing the α-amylase and α-glucosidase inhibitory activities was selected in this study. The changes of flavor profiles and functional components were characterized in basis of optimizing the fermentation process of chickpea milk fermented by L. rhamnosus FMBL L23004 CNN, and untargeted metabolomics, network pharmacology and molecular docking techniques were combined to explore the potential hypoglycemic components and their mechanisms in fermented chickpea milk. The results showed that the optimal process for the fermentation of chickpea milk were involved following parameters: 3% of the inoculation amount of L. rhamnosus FMBL L23004 CNN, fermentation temperature of 36.5 ℃, and fermentation duration of 17 h. L. rhamnosus FMBL L23004 CNN not only significantly enhanced the α-amylase and α-glucosidase inhibitory activity (30.96%), antioxidant capacity (ABTS+ radical scavenging activity: 72.39%, DPPH radical scavenging activity: 83.55%) and the contents of total phenols (30.46 mg/100 mL) and flavonoids (54.69 mg/100 mL) in chickpea milk through fermentation, but also improved the flavor by decomposing beany flavor substances such as 2-pentylfuran, octenal, and hexanal. Untargeted metabolomics revealed that the fermented chickpea milk was mainly composed of amino acids, peptides and analogues, fatty acids and conjugates, carbohydrate derivatives, terpenoids, and flavonoids. Fermentation of chickpea milk with L. rhamnosus FMBL L23004 CNN contributed to a significant increase in 73 pre-existing compounds and the formation of 775 novel compounds. The potential hypoglycemic effects of 10 key compounds were analyzed using network pharmacology and molecular docking. The results indicated that bioactive constituents, such as Diosgenin, Lepidium terpenoid, and Inulobiose, may synergistically exert hypoglycemic effects through the involvement of the PI3K-Akt signaling pathway, the lipid metabolism and atherosclerosis pathways, as well as the Ras signaling pathway. Notably, Diosgenin exhibited high docking stability with target proteins through hydrogen bonding interactions: STAT3 (−8.9 kcal/mol), AKT1 (−8.3 kcal/mol), α-amylase (−9.7 kcal/mol), and α-glucosidase (−8.8 kcal/mol). This findings provided valuable technical insights into chickpea resource utilization, while also establishing a theoretical foundation for the application of L. rhamnosus FMBL L23004 CNN in food and medicine.
126
54
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
